Molecular genetic analysis of human cystic hydatid cases from Poland: identification of a new genotypic group (G9) of Echinococcus granulosus

Parasitology ◽  
1997 ◽  
Vol 114 (1) ◽  
pp. 37-43 ◽  
Author(s):  
J. C. SCOTT ◽  
J. STEFANIAK ◽  
Z. S. PAWLOWSKI ◽  
D. P. McMANUS
Keyword(s):  
Echinococcus Granulosus ◽  
Fine Needle Aspiration Biopsy ◽  
Molecular Genetic ◽  
Needle Aspiration ◽  
Molecular Genetic Analysis ◽  
Genotypic Group ◽  
The Common ◽  
Mitochondrial Nd1 ◽  
Cystic Hydatid ◽  
Sheep Strain

We have used nuclear (ribosomal ITS1) and mitochondrial (ND1) sequences to characterize human and pig isolates of Echinococcus granulosus collected by fine needle aspiration biopsy (FNAB) in Poland. The data indicate clearly that the Polish patients were not infected with the common sheep strain (G1 genotype) of E. granulosus, normally associated with human cystic hydatid infection. Instead, the hydatid parasite infecting the Polish patients shares very similar ND1 sequence with the previously characterized pig (G7) genotype but it also exhibits some clear differences. In particular, E. granulosus DNA from the Polish patients amplified a single ITS1 fragment in PCR and distinct ITS1–RFLP patterns were obtained after restriction digestion. The form of hydatid isolated from the Polish patients appears, therefore, to represent a distinct, previously undescribed genotype (designated G9) of E. granulosus.

Genetic variation and epidemiology of Echinococcus granulosus in Argentina

Parasitology ◽  
1999 ◽  
Vol 118 (5) ◽  
pp. 523-530 ◽  
Author(s):  
M. C. ROSENZVIT ◽  
L.-H. ZHANG ◽  
L. KAMENETZKY ◽  
S. G. CANOVA ◽  
E. A. GUARNERA ◽  
...  
Keyword(s):  
Echinococcus Granulosus ◽  
Rflp Analysis ◽  
Santa Fe ◽  
Cystic Hydatid Disease ◽  
Rio Negro ◽  
Pcr Rflp ◽  
Control Programmes ◽  
The Common ◽  
Cystic Hydatid ◽  
Sheep Strain

Polymerase chain reaction–ribosomal ITS-1 DNA (rDNA) restriction fragment length polymorphism (PCR–RFLP) analysis and sequencing of the mitochondrial cytochrome c oxidase subunit 1 (CO1) and NADH dehydrogenase 1 (ND1) genes were used to characterize 33 Echinococcus granulosus isolates collected from different regions and hosts in Argentina, and to determine which genotypes occurred in humans with cystic hydatid disease. The results of the study demonstrated the presence of at least 4 distinct genotypes; the common sheep strain (G1) in sheep from Chubut Province and in humans from Río Negro Province, the Tasmanian sheep strain (G2) in sheep and 1 human from Tucumán Province, the pig strain (G7) in pigs from Santa Fe Province and the camel strain (G6) in humans from Río Negro and Buenos Aires Provinces. The finding that pigs harboured the pig strain and the occurrence of the Tasmanian sheep strain has considerable implications for the implementation of hydatid control programmes due to the shorter maturation time of both strains in dogs compared with the common sheep strain. Furthermore, this is the first report of the presence of the G2 and G6 genotypes in humans which may also have important consequences for human health.

scholarly journals Regularities of Cholera Spread in the Volga Basin in 1970-1973

2010 ◽  
pp. 22-27 ◽  
Author(s):  
A. I. Kologorov ◽  
O. V. Kedrova ◽  
D. A. Pakhomov ◽  
N. V. Piskunova ◽  
A. I. Kovtunov ◽  
...  
Keyword(s):  
Genetic Analysis ◽  
Retrospective Analysis ◽  
Water Transport ◽  
Molecular Genetic ◽  
Early Period ◽  
Molecular Genetic Analysis ◽  
Common Origin ◽  
Main Role ◽  
Permanent Residence ◽  
The Common

Retrospective analysis of cholera spread in the early period of the seventh pandemic in Russia (1970-1973) has been carried out. It was elucidated that importation and further spread of cholera in the Astrakhan region in 1970 had been realized through the water way. The development of a large cholera focus in the territory of this region determined the unfavorable situation as regards this infection in the whole country in the period of 1970-1972. Water transport was shown to play the main role in cholera spread. The risk cohorts were the population of the Astrakhan region, shipboard personnel of the Volga steamship company, fishermen, persons without permanent residence. Molecular-genetic analysis of cholera agent strains isolated from the patients (vibriocarriers) and the environment showed that they belonged to one clone, thus the common origin of cholera cases in the basin of the Volga was confirmed.

scholarly journals Employing NADH Dehydrogenase Subunit 1 in the Determination of Echinococcus granulosus Strain in Sheep, Cattle and Human in Thi-Qar Province, Iraq

2021 ◽  
Vol 18 (2) ◽  
pp. 0238
Author(s):  
Sarmad Awad Mozan AL-Asadi ◽  
Wesam Jasim Hansh ◽  
Abdul-Hussien Habash Awad
Keyword(s):  
Echinococcus Granulosus ◽  
Molecular Study ◽  
Molecular Techniques ◽  
Hydatid Cysts ◽  
Intermediate Hosts ◽  
Pcr Products ◽  
Nad1 Gene ◽  
The Common ◽  
Sheep Strain

Echinococcosis is a zoonotic disease caused by the larval stage of the tapeworm Echinococcus granulosus. This disease is an important public health and a significant economic issue in Iraq, where the lungs and livers are the popular places of infection. The aim of the current study focused on using the molecular techniques in the detection of an E. granulosus strain that causes cystic echinococcosis to human, sheep and cattle in Thi-Qar province, Iraq. In the current study, thirty isolates of E. granulosus were collected from 10 human hydatid cysts through surgery done at Al-Hussein Imam Teaching Hospital in Thi-Qar province and 10 sheep with 10 cattle hydatid cysts were obtained from the slaughterhouse in Thi-Qar province, Southern of Iraq to identify strains of E. granulosus which infect human and other intermediate hosts  (sheep and cattle). The molecular study was carried out on the isolates and a specific primer set for the mitochondrial dehydrogenase NADH subunit 1 (NAD1) gene was used. This primer set was amplified 400 bp of the NAD1 gene in all selected isolates. The PCR products for the twelve selected isolates of E. granulosus (4 isolates per intermediate host) were sequenced and the results for these twelve isolates showed that all sequenced isolates, except one isolate Eg_5, belonged to the sheep strain G1 and a slight genetic diversity was observed with the reference sequences of the strain G1. The exception was in the isolate Eg_5 isolated from a cattle liver, which was similar to the buffalo strain G3. This study concludes that the common E. granulosus strain in Thi-Qar province is G1.  

Echinococcus granulosus: intraspecific genetic variation assessed by a DNA repetitive element

Parasitology ◽  
2001 ◽  
Vol 123 (4) ◽  
pp. 381-388 ◽  
Author(s):  
M. C. ROSENZVIT ◽  
S. G. CANOVA ◽  
L. KAMENETZKY ◽  
E. A. GUARNERA
Keyword(s):  
Genetic Variation ◽  
Echinococcus Granulosus ◽  
Great Degree ◽  
Copy Number ◽  
Repetitive Element ◽  
Cystic Hydatid Disease ◽  
And Control ◽  
Cystic Hydatid ◽  
Cattle Strain ◽  
Sheep Strain

A 186 bp Echinococcus granulosus-specific repetitive element, TREg, was used to assess genetic variation between strains. In G7 genotype (pig strain) it has the characteristics of a satellite DNA element with a copy number of 23000 per haploid genome. Analysis, by sequencing of TREg monomers, showed a great degree of identity within them. In the G1 genotype (common sheep strain) TREg-like repetitive elements were found in an interspersed distribution throughout the genome and in only 120 copies. The sequences of these monomers showed a great degree of variation between them and with TREg of G7 origin. The G6 genotype (camel strain) showed a pattern of distribution and copy number similar to the G7 genotype, and the G2 genotype (Tasmanian sheep strain) similar to the G1 genotype. Isolates from the G5 (cattle strain) and G4 (horse strain) genotypes also showed unique hybridization patterns in Southern blot experiments. The genomic plasticity of E. granulosus, which may have important consequences in the epidemiology and control of cystic hydatid disease is reflected in the results of this work.

scholarly journals Morphological and molecular genetic analysis of Synhimantus (Synhimantus) laticeps (Rudolphi, 1819) (Nematoda, Acuariidae) from the barn owl (Tyto alba) and the common kestrel (Falco tinnunculus) in Austria

2017 ◽  
Vol 54 (3) ◽  
pp. 262-269 ◽  
Author(s):  
D. Ebmer ◽  
H.-P. Fuehrer ◽  
B. Eigner ◽  
H. Sattmann ◽  
A. Joachim
Keyword(s):  
Molecular Genetic ◽  
First Record ◽  
Barn Owl ◽  
Molecular Genetic Analysis ◽  
Genetic Identification ◽  
Coi Gene ◽  
Tyto Alba ◽  
Electron Microscopic ◽  
Barn Owls ◽  
The Common

SummaryIn the framework of the biodiversity initiative and barcoding project “Austrian Barcode of Life” (ABOL) post mortem examinations of the gastro-intestinal tracts of different species of wild birds were carried out and several adult helminths were retrieved. In the gizzard of two barn owls (Tyto alba) and one common kestrel (Falco tinnuculus) acuariid nematodes belonging to the species Synhimantus (Synhimantus) laticeps (Rudolphi, 1819) were discovered. This report illustrates the identification of this parasitic nematode by morphometric comparison and scanning electron microscopic photographs. Furthermore, genetic identification of individual parasites based on a fragment of the mitochondrial cytochrome c oxidase subunit I (COI) gene and the nuclear 18S ribosomal RNA gene was carried out. This report constitutes the first COI-based DNA barcoding of S. (S.) laticeps and its first record in the barn owl (Tyto alba) in Austria.

Relative expression of the 14-3-3 gene in different morphotypes of cysts of Echinococcus granulosus isolated from the Indian buffalo

2010 ◽  
Vol 84 (4) ◽  
pp. 394-397 ◽  
Author(s):  
D. Pan ◽  
A.K. Bera ◽  
S. De ◽  
S. Bandyopadhyay ◽  
S.K. Das ◽  
...  
Keyword(s):  
Amino Acid ◽  
Amino Acid Sequence ◽  
Echinococcus Granulosus ◽  
Predicted Amino Acid Sequence ◽  
Germinal Layer ◽  
Relative Expression ◽  
The Common ◽  
Sheep Strain

AbstractThe metacestode stage of Echinococcus granulosus is of zoonotic importance. In general, the 14-3-3 protein is involved in multiplication and survival of eukaryotes. Therefore, this communication presents succinct information on relative expression of the 14-3-3 protein in six different morphotypes of cysts of E. granulosus. All isolated E. granulosus belonged to the common sheep strain (G1). Relative expression of the 14-3-3 protein was higher in fertile cysts when compared to sterile cysts. The predicted amino acid sequence of the 14-3-3 protein was closely clustered with zeta-type isoforms 1 and 2 of the 14-3-3 protein. In addition, the present study demonstrates the presence of the 14-3-3 protein which until now had not been detected in the germinal layer. Our findings indicate that the expression of this biomolecule in the germinal layer of sterile organisms may contribute to the development and survival of the parasite in the host. The uniform expression of actin II conclusively proves the survivability of the harvested organisms.

Molecular classification of Echinococcus granulosus strains from livestock animals in Libya

2016 ◽  
Vol 3 (4) ◽  
pp. 462-470
Author(s):  
MF Wajdii ◽  
M. F. Wajdii ◽  
W Rahman ◽  
W. Rahman ◽  
O Elmajdoub ◽  
...  
Keyword(s):  
Microsatellite Markers ◽  
Cryptic Species ◽  
Echinococcus Granulosus ◽  
Molecular Classification ◽  
Final Host ◽  
Molecular Tools ◽  
Intermediate Hosts ◽  
The Common ◽  
Sheep Strain

This study demonstrates the distribution of various E. granulosus strains in sheep, cattle and camel. The common sheep strain G1 is mainly found in sheep and cattle, but also parasitized camels. In contrast, the camel strain G6 is found mainly in camels and rarely in sheep and cattle. However, the study also revealed the possible presence of cryptic species that are closely related to both genotypes in livestock of Libya as evident by high mutations in several specimens. Based on the occurrence of overlapping hosts of E. granulosus in Libya, more research on the transmission cycles and genotypes of E. granulosus in Libya is required. In addition, it is suggested that surveys on potential intermediate hosts, including in humans with dogs as the major final host in Libya using higher resolution molecular tools such as microsatellite markers is recommended.

scholarly journals Identification of Echinococcus granulosus strains using polymerase chain reaction–restriction fragment length polymorphism amongst livestock in Moroto district, Uganda

2016 ◽  
Vol 83 (1) ◽  
Author(s):  
Martin Chamai ◽  
Leonard Omadang ◽  
Joseph Erume ◽  
Michael Ocaido ◽  
Peter Oba ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Restriction Fragment Length Polymorphism ◽  
Echinococcus Granulosus ◽  
Length Polymorphism ◽  
Fragment Length Polymorphism ◽  
Chain Reaction ◽  
The Common ◽  
Polymerase Chain ◽  
Sheep Strain ◽  
Restriction Fragment Length

A descriptive study was conducted to identify the different strains of Echinococcus granulosus occurring in livestock in Moroto district, Uganda. Echinococcus cysts from 104 domestic animals, including cattle, sheep, goats and camels, were taken and examined by microscopy, polymerase chain reaction with restriction fragment length polymorphism and Sanger DNA sequencing. Echinococcus granulosus genotypes or strains were identified through use of Bioinformatics tools: BioEdit, BLAST and MEGA6. The major finding of this study was the existence of a limited number of E. granulosus genotypes from cattle, goats, sheep and camels. The most predominant genotype was G1 (96.05%), corresponding to the common sheep strain. To a limited extent (3.95%), the study revealed the existence of Echinococcus canadensis G6/7 in three (n = 3) of the E. granulosus–positive samples. No other strains of E. granulosus were identified. It was concluded that the common sheep strain of Echinococcus sensu stricto and G6/7 of E. canadensis were responsible for echinococcal disease in Moroto district, Uganda.

scholarly journals Ancient variants of the Epstein–Barr virus (Herpesviridae, Lymphocryptovirus, HHV-4): hypotheses and facts

2020 ◽  
Vol 65 (2) ◽  
pp. 77-86
Author(s):  
Kseniya V. Smirnova ◽  
Natalya B. Senyuta ◽  
Alexandra K. Lubenskaya ◽  
Tatyana E. Dushenkina ◽  
Vladimir E. Gurtsevich
Keyword(s):  
Oral Cavity ◽  
Ethnic Groups ◽  
Epstein Barr Virus ◽  
Genetic Relationships ◽  
Molecular Genetic ◽  
Molecular Genetic Analysis ◽  
Barr Virus ◽  
Evolution Of Life ◽  
The Common ◽  
Epstein Barr

Introduction. Molecular studies have shown that viruses appeared in the early stages of the evolution of life. For millions of years, viruses have evolved by changing old and acquiring new sequences in their RNA or DNA. It is assumed that most viruses have common ancestors. Such an ancestor, an ancient strain, probably existed for Epstein-Barr virus (EBV) as well.Aim. To find out whether ancient strains of EBV persist in modern Russian ethnic groups today.Material and methods. The object of the study was the EBV LMP1oncogene, which is most suitable for molecular genetic analysis. LMP1 was amplified from the oral cavity washings obtained from representatives of two ancient ethnic groups of Russia - Tatars and Slavs. The LMP1 amplicons were sequenced in both directions; their nucleotide sequences translated into amino acid (LMP1) were evaluated using the classification suggested by Edwards et al. 1999. To establish genetic relationships between LMP1 variants, a phylogenetic tree was constructed by the neighbor-joining method using the MEGA software package.Results and discussion. Analysis of LMP1 sequences from washings of the Slavs oral cavity demonstrated the presence of LMP1 variants with varying degrees of transforming potential: B98.5/A, China1, Med-, and NC. The analysis of LMP1 sequences from washings of Tatar oral cavity also made it possible to identify oncoprotein variants such as B95.8/A, China1, Med-, as well as a group of variants out of classifications (LMP1-OK). An important finding was the identification of 7 variants of LMP1 from Tatars, designated as LMP1-TatK, that contained two unique deletions of 5 aa in codons 312-316 and 382-386, which were absent in the LMP1 variants from Slavs and from previously examined cancer patients and healthy individuals, as well as in sequences from open computer databases. The uniqueness of the LMP1-TatK variant is confirmed both by phylogenetic analysis of LMP1 sequences of Tatar origin and by the analysis of 11 aa repeats and 5 aa insertions in the C-terminal region of the oncoprotein. The morbidity and mortality rates from neoplasms, including EBV-associated pathologies, did not differ significantly between two studied ethnic groups infected with different EBV strains.Conclusion. The data obtained allowed us to suggest that: 1) LMP1-TatK could be refered to an evolutionarily ancient EBV strain that persists among Tatars and; 2) LMP1-TatK belongs to the so-called “Volga” EBV virus strain, the common strain among the population of the Volga region. Extended studies of EBV isolates from residents of this region may probably shed the light on the origin of LMP1-TatK.

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