Electron Microscopic Studies of Gerbil Testis After Vasectomy

1976 ◽  
Vol 34 ◽  
pp. 154-155
Author(s):  
S. K. MAJUMDAR ◽  
FRED KALENSCHER
Keyword(s):  
Electron Microscope ◽  
Normal Structure ◽  
Uranyl Acetate ◽  
Meriones Unguiculatus ◽  
Mongolian Gerbils ◽  
Electron Microscopic ◽  
Transmission Electron ◽  
Acetate Lead ◽  
Microscopic Studies ◽  
Ultrathin Sections

Ultrathin sections made from bilaterally vasectomized as well as bilaterally sham-operated Mongolian gerbils (Meriones unguiculatus) were examined and compared under an electron microscope in order to determine whether vasectomy has any effect upon the fine structure of the testis. The whole testes were removed and placed in Karnovsky's fixative for one hour. After this period the testes were diced into small pieces and fixed for an additional hour in the same fixative. After rinsing in distilled water and postfixed for one hour in OsO4, the tissues were embedded in Epon 812. The sections were stained with uranyl acetate-lead citrate and examined on a Philips Model 201 transmission electron microscope. Shamoperated testis exhibited normal structure of germ cells.

Electron-Microscopic localization of biotinylated gastrin bound to eosinophils in the rat stomach

1994 ◽  
Vol 52 ◽  
pp. 300-301
Author(s):  
Caroline A. Miller ◽  
David H. Nichols ◽  
Richard F. Murphy
Keyword(s):  
Electron Microscope ◽  
Phosphate Buffer ◽  
Uranyl Acetate ◽  
List Type ◽  
Cerium Chloride ◽  
Rat Stomach ◽  
Electron Microscopic ◽  
Human Sequence ◽  
Acetate Lead ◽  
Microscope Slides

Gastrin is a small peptide capable of both stimulating gastric acid secretion and acting as an enteric growth factor. Known functions of eosinophils in the rat stomach are related to immunological defense. Here we demonstrate the binding of biotinylated gastrin to rat stomach eosinophils in the electron microscope. Small pieces of stomach were fixed by immersion in 4% paraformaldehyde/0.1% glutaraldehyde in 0.1 M phosphate buffer, pH 7.4 for 1 hour. The tissue was then cryoprotected in 30% sucrose/0.1 M phosphate buffer, transferred to Tissue Tek OCT compound and frozen in isopentane cooled with liquid nitrogen. Transverse cryostat sections were cut at 25 μm, thawed in PBS and free floating sections exposed to 10−5 M biotinylated 1-17 gastrin (human sequence; Peninsula Labs) for 1 hour. Controls omitted the biotinylated gastrin from this step. Sections were then rinsed 3X in PBS and exposed to either:1).a 1:50 dilution of 10 nm Extravidin colloidal gold (Sigma) for 2 hours, or2).an avidin-biotin-alkaline phosphatase complex (ABC-AP;Vector) for 1 hour. A substrate solution containing cerium chloride was used to generate an electron dense reaction product.Sections from both procedures were postfixed in 1% OsO4 in 0.1 M phosphate buffer, rinsed and dehydrated. These were then flat embedded in EMbed 812 between two microscope slides coated with Liquid Release (both from Electron Microscopy Sciences).Polymerized sections were adhered to resin blocks using super glue, cut at 70-90 nm, stained with uranyl acetate/lead citrate and observed in a Philips CM-10 electron microscope.

Ultrastructural Changes of Gerbil Epididymis after Vasectomy

1977 ◽  
Vol 35 ◽  
pp. 672-673
Author(s):  
Shyamal K. Majumdar ◽  
Marion Shapiro ◽  
J. Gary Caputi
Keyword(s):  
Ventral Side ◽  
Uranyl Acetate ◽  
Meriones Unguiculatus ◽  
Ultrastructural Changes ◽  
Sham Operation ◽  
Mongolian Gerbils ◽  
Additional Information ◽  
Ultrastructural Studies ◽  
Transmission Electron ◽  
One Year

Ultrastructural studies of gerbil testes after vasectomy have been described (2S3). The present study was undertaken to obtain additional information on the effects of vasectomy in the fine structures of the epididymis of the Mongolian gerbils (Meriones unguiculatus). The animals were subjected to vasectomy or sham operation at intervals up to one year. An incision was made on the ventral side of the animal just above the scrotum, and about one centimeter of each vas deferens was removed and the ends were firmly ligated. The cauda epididymis of vasectomized and sham operated animals was fixed for two hours in Karnovsky's fixative. Following a rinse in buffer, the tissue was post fixed for one hour in osmium tetroxide, dehydrated, and embedded in Epon 812. The sections were stained with uranyl acetate followed by Reynolds' lead citrate, and examined with a Philips 201 transmission electron microscope operating at 60 kV.

Organs of a Baikal seal affected by a morbillivirus

1990 ◽  
Vol 48 (3) ◽  
pp. 968-969
Author(s):  
O.I. Belykh ◽  
Ye.V. Likhoshway ◽  
Yu.V. Solodun ◽  
O.A. Goldberg ◽  
V.P. Kumarev
Keyword(s):  
Electron Microscopy ◽  
Measles Virus ◽  
Present Report ◽  
Protein A ◽  
Uranyl Acetate ◽  
Kidney Cells ◽  
Electron Microscopic ◽  
Microscopic Studies ◽  
Liver And Kidney ◽  
Ultrathin Sections

The population of Baikal seals Phoca sibirica has been plagued in 1987-88 by an unknown disease. Oligonucleotide probing of nucleic acids isolated from tissues of ill and dead animals, as well as immunological evidence and clinical data suggested that seals were infected by a morbillivirus. Morbillivirus antigen has been vizualized in dead seal tissues by immunoelectron microscopy (preembedding technique).The present report gives outline of electron microscopic studies of the tissues of infected Baikal seals. Morbillivirus antigens were vizualized as clusters of gold spheres by postembedding technique with monoclonal antibodies against measles virus and protein A-colloid gold conjugates in nuclei and cytoplasm of liver and kidney cells. Some clusters were associated with virus-like particles having a diameter of 80-100 nm. Electron microscopy of ultrathin sections stained with uranyl acetate revealed nucleocapsides having length of up to 1400 nm, and a diameter of 13-17 nm, morphologically similar to measles and seals distemper virus.

Ultrastructural observations of the ciliated cyst in the human uterine tube epithelium

1990 ◽  
Vol 48 (3) ◽  
pp. 104-105
Author(s):  
Haruo Hagiwara ◽  
Susumu Shibasaki ◽  
Nobuo Ohwada
Keyword(s):  
Phosphate Buffer Solution ◽  
Uranyl Acetate ◽  
Buffer Solution ◽  
Solution Ph ◽  
Uterine Tube ◽  
Ultrastructural Studies ◽  
Transmission Electron ◽  
Gynecological Diseases ◽  
Microscopic Studies ◽  
Ultrathin Sections

The ciliated cysts have been identified as a common manner of development of ciliated cells through light microscopic studies, however recent ultrastructural studies have Reported that they should be regarded as an expression of atypical ciliogenesis. In the present study we attempt to elucidate the detailed structure of the ciliated cysts and their significance in ciliogenesis in the human uterine tube epithelium.Human uterine tubes were obtained from 20 women who underwent total hysterectomies due to gynecological diseases. The ampullar segments were cut into small pieces, and fixed in 2.5% glutaraldehyde-2% paraformaldehyde (1/2 Kamovsky) in a 0.1M phosphate buffer solution (pH 7.4). These specimens were postfixed in a 1% Osmium tetroxide solution (Millonig), dehydrated with a graded series of ethanol and embedded in Epon 812. Ultrathin sections were stained with uranyl acetate and lead citrate, and examined in a transmission electron microscope.The ciliated cysts were detected as a round or oval intracytoplasmic cavity provided with many ciliary apparatuses and microvilli (Fig. 1).

Adrenal gland ultrastructural pathology in plasmodium berghei parasitized mice

1996 ◽  
Vol 54 ◽  
pp. 748-749
Author(s):  
M. Pulido-Méndez ◽  
H.J. Finol ◽  
A. Rodríguez-Acosta ◽  
A. Márquez ◽  
I. Aguilar ◽  
...  
Keyword(s):  
Electron Microscope ◽  
Adrenal Gland ◽  
Adrenal Cortex ◽  
Plasmodium Berghei ◽  
Liver Pathology ◽  
Male Mice ◽  
Electron Microscopic ◽  
Malaria Research ◽  
Transmission Electron ◽  
Microscopic Studies

The use of animal models is widely accepted in malaria research. Plasmodium berghei has been extensively studied because it produces a mortal malaria in rodents. Electron microscopic studies on brain and liver pathology have been performed in this infection. In a previous ultrastructural work we reported the capillary alterations observed in adrenal cortex in P. berghei infected mice. In this work we describe the cell and capillary changes we found in adrenal cortex and medulla in this infection.Male mice weighing 18-22 g were inoculated intraperitoneally with P. berghei infected erythrocytes. At the ninth day animals were sacrificed when parasitemia ranged between 80-90%. Adrenal gland samples were processed by routine techniques and observed in a Hitachi H-500 transmission electron microscope.

Electron microscopic study on the phagocytic lining cells in the cavernous body of the lamprey gill

1978 ◽  
Vol 36 (2) ◽  
pp. 448-449
Author(s):  
Kazuhito Yamaguchi ◽  
Kazuhiko Awaya
Keyword(s):  
Electron Microscope ◽  
Uranyl Acetate ◽  
Osmic Acid ◽  
Lead Citrate ◽  
Electron Microscopic ◽  
Embedded Blocks ◽  
Ultrathin Sections ◽  
Branchial Artery ◽  
Scanning Electron ◽  
Cavernous Body

The lining cells in the cavernous body, which is a branch of the afferent branchial artery, of lamprey gills were studied with transmission (TEM) and scanning electron microscope (SEM).Adult and larval lampreys, Lampertra planeri, were used in this study. Lamprey gills removed after perfusion were fixed in 2 % glutaraldehyde/formaldehyde (pH 7. 4) for 1 hour and postfixed in 1 % osmic acid. For TEM, the gills were dehydrated in ethanol and embedded in epon 812. Ultrathin sections were doubly stained with uranyl acetate and lead citrate. For SEM, most of the gills were dehydrated in ethanol and isoamylacetate and cracked in liquid nitrogen. Some of the gills dehydrated were embedded in stylene monomer. Stylene embedded blocks were cracked with a hummer and dissolved in propylene oxide. All the specimens were dried in a critical point dryer and coated 100 Å thickness with gold-paladium.The cavernous body is composed of trabeculae, between which are blood spaces, the lacunae.

Ultrastructure of Developing Granulosa and Theca Cells

1970 ◽  
Vol 28 ◽  
pp. 92-93
Author(s):  
Iracema M. Baccarini
Keyword(s):  
Electron Microscope ◽  
Osmium Tetroxide ◽  
Uranyl Acetate ◽  
Lead Citrate ◽  
Electron Microscopic ◽  
Ether Anesthesia ◽  
Rat Fetuses ◽  
Theca Cells ◽  
The Past ◽  
Microscopic Studies

The embryology of granulosa and theca cells is not understood thoroughly. Electron microscopic studies in the past have been concerned mainly with mature granulosa cells and less with their development.Material and Methods. Rat fetuses were removed surgically under ether anesthesia at 16-17, 17-18 and 18-19 days of gestation. Their abdominal cavities were opened, and the fetuses were placed immediately into 3% glutaraldehyde (pH 7.2) for 3 hours. During this time, the fetal ovaries were dissected under a microscope. The tissue was washed in phosphatebuffer for 24 hours, post-fixed in 1% phosphate buffered osmium tetroxide for 1-2 hours at 4°C, and embedded in Durcupan ACM (Fluka). Sections were double stained with uranyl acetate and lead citrate, and viewed in an RCA-EMU-3D electron microscope.

Electron microscopic studies on the postnatal growth of mouse iridocorneal angle

1978 ◽  
Vol 36 (2) ◽  
pp. 606-607
Author(s):  
K. Yoshida ◽  
F. Murata ◽  
S. Ohno ◽  
T. Nagata
Keyword(s):  
Developmental Process ◽  
Postnatal Growth ◽  
Uranyl Acetate ◽  
Electron Microscopic ◽  
Iridocorneal Angle ◽  
Microscopic Studies ◽  
Ultrathin Sections ◽  
Important Position ◽  
Flat Embedding ◽  
Outflow Pathway

The iridocorneal angle(ICA) occupies an important position in the eye from the viewpoint of the aqueous outflow pathway. There are several studies on the iridocorneal angle formation of the mammalian eyes, such as the rat, monkey and man. However, there is no paper available on that of the mouse. The purpose of this study is to report the developmental process of the iridocorneal angle formation in the mouse eye.Albino mice of various ages from newborn to adult were used in this study. The ages of mice were 3, 5, 7, 9, 10, 11, 14 days old, 1, 6 and 12 months old. At least three individual animals were studied at each developing stage. After decapitation, the eyes were quickly enucleated, and were prefixed in 2. 5% glutaraldehyde in 0. 1 M phosphate buffer (pH 7. 4) for three hours. They were then measured at their diameters before postfixation in 1% osmium tetroxide for two hours, dehydrated in a graded series of ethanol and embedded in Epon with a flat embedding mold to ensure their direction. Ultrathin sections were cut with an LKB Ultrotome, placed on the collodion coated grids and stained with uranyl acetate and lead citrate before observation with a Hitachi HS-9 electron microscope.

Electron microscopy of the changes in the sperm head curvature of the palm squirrel (Funambulus Penanti)

1993 ◽  
Vol 51 ◽  
pp. 334-335
Author(s):  
S. R. Bawa ◽  
R. Bawa ◽  
H. K. Bains
Keyword(s):  
Electron Microscopy ◽  
Plasma Membrane ◽  
Electron Microscope ◽  
Osmium Tetroxide ◽  
Uranyl Acetate ◽  
Sperm Head ◽  
Lead Citrate ◽  
Transmission Electron ◽  
Ultrathin Sections ◽  
Epididymal Spermatozoa

Examination of ultrathin sections of the spermatozoa recovered from the epididymis of the Indian palm squirrel (Funambulus penanti) indicates that the sperm head undergoes changes in its curvature during epididymal transit.Testis and epididymis of an adult male squirrel were dissected and small pieces of tissue fixed in 2.0% glutaraldehyde in 0.1 M phosphate buffer and post-fixed in osmium tetroxide. After dehydration in graded acetone the material was embedded in Araldite. Ultrathin sections were cut on a Reichert Jung Ultracut, picked-up on copper grids, stained with Reynold’s lead citrate-uranyl acetate and examined with a JEOL 1200 EX transmission electron microscope.Ultrathin sections of the caput epididymal spermatozoa reveal that their plasma membrane is adherent to the underlying acrosome (Figure 1). When these spermatozoa reach the corpus epididymis the plasma membrane surrounding the head becomes ruffled (Figure 2). The lifting-up of the plasma membrane around the head is restricted to the posterior bend of the acrosome.

A Reproducible Selective Stain for Cardiac Sarcoplasmic Reticulum

1974 ◽  
Vol 32 ◽  
pp. 214-215
Author(s):  
R. A. Waugh ◽  
J. R. Sommer
Keyword(s):  
Complex System ◽  
Electron Microscope ◽  
Sarcoplasmic Reticulum ◽  
Transmission Electron Microscope ◽  
Electron Microscopic ◽  
Cardiac Sarcoplasmic Reticulum ◽  
Transmission Electron

Cardiac sarcoplasmic reticulum (SR) is a complex system of intracellular tubules that, due to their small size and juxtaposition to such electron-dense structures as mitochondria and myofibrils, are often inconspicuous in conventionally prepared electron microscopic material. This study reports a method with which the SR is selectively “stained” which facilitates visualizationwith the transmission electron microscope.

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