Transmission Electron Microscopy of Endometrium in Women Bearing Progesterone- Releasing Intrauterine Devices

1975 ◽  
Vol 33 ◽  
pp. 604-605
Author(s):  
A. González-Angulo ◽  
R. Aznar-Ramos ◽  
I. Ruiz de Chavez
Keyword(s):  
Release Rate ◽  
Ultrastructural Level ◽  
Intrauterine Devices ◽  
Cell Organelles ◽  
Continuous Release ◽  
Reduced Frequency ◽  
Transmission Electron ◽  
Proliferative Endometrium ◽  
Secretory Endometrium ◽  
Natural Progesterone

The addition of natural progesterone with a continuous daily release rate to inert intrauterine devices has resulted in a lower pregnancy rate and a reduced frequency of bleeding and expulsions. Light microscopy studies on these endometria have shown a suppressed or inactive pattern in addition to inflammatory infiltrate as well as a diffuse predecidual reaction with degenerative changes in most cases. The aim of the present study is to characterize at ultrastructural level the possible modifications seen in cell organelles in both epithelium and stroma resulting from a continuous release rate of progesterone.Twenty endometrial biopsies were taken; ten at day 10 ± 2 and ten at 20 ± 2 days of the menstrual cycle. Proliferative endometrium disclosed a paucity of cell organelles and it was similar to what has been described for inert devices (2). Secretory endometrium disclosed accumulation of light and dark glycogen in the cytoplasm (Fig. 1).

Migration of human neutrophils in hydrated collagen lattices

1982 ◽  
Vol 58 (1) ◽  
pp. 95-108
Author(s):  
F. Grinnell
Keyword(s):  
Ultrastructural Level ◽  
Human Neutrophils ◽  
Inert Material ◽  
Collagen Fibres ◽  
Cell Organelles ◽  
Forward Movement ◽  
Cytoskeletal Network ◽  
Transmission Electron ◽  
Plastic Surfaces ◽  
Collagen Lattices

The migration of human neutrophilic granulocytes in hydrated collagen lattices was studied by a combination of cinemicroscopy, and scanning and transmission electron microscopy. The basic pattern of cell migration in collagen was similar to that observed previously for these cells on inert material surfaces; i.e., a cycle of cell extension and cytoplasmic flow into the leading extension. In general, however, neutrophils in collagen were less spread than on glass or plastic surfaces. Thin lamellipodia were absent and the leading extension of the cells was often an elaborately folded pseudopodium. In addition, neutrophils migrating in collagen were never observed to have retraction fibres at the tail end of the cells, although a uropod was usually seen. In the region of the uropod, extensive blebbing of the cells often occurred, and when this happened, forward movement of the cells ceased. At the ultrastructural level, both the leading pseudopodium and the blebs at the tail of the cell were found to contain a dense cytoskeletal network from which cell organelles were excluded. Finally, the cells were found to be coated with an extensive glycocalyx, and individual collagen fibres were sometimes observed within the glycocalyx.

Routine Syndecan-1 Immunohistochemistry Aids in the Diagnosis of Chronic Endometritis

2004 ◽  
Vol 128 (9) ◽  
pp. 1000-1003 ◽  
Author(s):  
Ilene B. Bayer-Garner ◽  
Jennifer A. Nickell ◽  
Soheila Korourian
Keyword(s):  
Plasma Cells ◽  
Abnormal Uterine Bleeding ◽  
Endometrial Biopsy ◽  
Uterine Bleeding ◽  
Endometrial Polyp ◽  
Chronic Endometritis ◽  
Underlying Malignancy ◽  
Proliferative Endometrium ◽  
Exogenous Progesterone ◽  
Secretory Endometrium

Abstract Context.—Chronic endometritis is reportedly observed in 3% to 10% of women undergoing endometrial biopsy for abnormal uterine bleeding. The diagnosis of chronic endometritis rests on the identification of the plasma cells. Their identification may be obscured by a mononuclear cell infiltrate, plasmacytoid stromal cells, abundant stromal mitoses, a pronounced predecidual reaction in late secretory endometrium, menstrual features, or secondary changes due to exogenous progesterone treatment prior to the biopsy. Syndecan-1 is a proteoglycan that is found on the cell surface of plasma cells and keratinocytes. Immunohistochemistry stains for this antibody may facilitate diagnosis of chronic endometritis. Objective.—To determine whether or not routine syndecan-1 immunohistochemistry will aid in the diagnosis of chronic endometritis. Design.—Immunohistochemistry stains for syndecan-1 were performed on 3 levels of 47 endometrial biopsies from patients with abnormal uterine bleeding. None of the patients had endometrial hyperplasia or an underlying malignancy. Clinical correlation and follow-up was attempted in 20 cases that showed evidence of plasma cells by syndecan-1 by immunohistochemistry. Results.—Plasma cells were identified in 20 cases, 7 of which were initially diagnosed as chronic endometritis. The remaining 13 positive cases were diagnosed as tubal metaplasia (1), secretory endometrium (4), proliferative endometrium (4), menstrual endometrium (1), endometrial polyp (1), secretory endometrium with endometrial polyp (1), and endometrial polyp with exogenous hormone effect (1) based on the original hematoxylin-eosin section. Conclusions.—Syndecan-1 may be a useful adjunct in the diagnosis of chronic endometritis. Approximately half of the cases of chronic endometritis responded to an antibiotic regime; thus, this diagnosis is important and may potentially obviate the need for surgical intervention.

The Ultrastructure and Ontogeny of Pollen in Helleborus Foetidus L

1968 ◽  
Vol 3 (2) ◽  
pp. 161-174
Author(s):  
P. ECHLIN ◽  
H. GODWIN
Keyword(s):  
Cell Wall ◽  
Tapetal Cell ◽  
Pollen Grains ◽  
Ultrastructural Level ◽  
Low Molecular Weight ◽  
Cell Organelles ◽  
Helleborus Foetidus ◽  
Ubisch Bodies ◽  
Ubisch Body ◽  
Development Proceeds

The ontogeny of the tapetum and Ubisch bodies in Helleborus foetidus L. has been examined at the ultrastructural level, and their development has been closely linked with that of the sporogenous cell and pollen grains. During development the tapetum passes through successive phases of synthesis, maturity and senescence, ending in complete dissolution. During the anabolic phase of growth, precursors of the Ubisch bodies are formed as spheroidal vesicles of medium electron density within the tapetal cytoplasm; they are associated with a zone of radiating ribosomes, which, as development proceeds, can clearly be seen to be situated on strands of endoplasmic reticulum. The callose special wall round the microspores and the tapetal cell wall now disintegrate and the pro-Ubisch bodies are extruded through the cell membrance of the tapetal cells, where they remain on the surface of the anther cavity and soon become irregularly coated with sporopollenin. Deposition of sporopollenin continues on the Ubisch bodies at the same time as upon the exines of the developing pollen grains. In both cases, the later stages of sporopollenin deposition are associated with electron-transparent layers of unit-membrane dimensions appearing in section as white lines of uniform thickness. Continuing deposition of sporopollenin leads to the formation of compound or aggregate Ubisch bodies. It is conjectured that the sporopollenin is synthesized from the compounds of low molecular weight released into the anther loculus by the breakdown of the callose special wall and the tapetal cell wall. The final stages of tapetal autolysis involve the disappearance of all the cell organelles. An attempt is made to relate the findings to those described in other recent studies on Ubisch body formation and to combine them in a common ontogenetic pattern.

A simple technique for staining of cell membranes with imidazole and osmium tetroxide.

1995 ◽  
Vol 43 (10) ◽  
pp. 1079-1084 ◽  
Author(s):  
G Thiéry ◽  
J Bernier ◽  
M Bergeron
Keyword(s):  
Osmium Tetroxide ◽  
Cell Membranes ◽  
Cell Organelles ◽  
Unsaturated Lipids ◽  
Transmission Electron ◽  
Glutaraldehyde Solution ◽  
Kidney Liver ◽  
The Relationship ◽  
Resin Penetration

We describe a simple new technique based on the affinity of imidazole and osmium tetroxide for unsaturated lipids. Organs (e.g., kidney, liver, intestine) were perfused in vivo with a glutaraldehyde solution. Tissue fragments were then immersed in a solution containing imidazole and OsO4 and are further stained with a double lead and copper citrate solution. Ultra-thin (0.06 microns) or thick (0.1-0.3 microns) sections were observed with transmission electron microscopy (80-100 kV). The method presented permits excellent visualization of cell membranes (e.g., endoplasmic reticulum, endocytotic apparatus) because it favors good resin penetration and the alkaline pH preserves cell volume. A better stereomicroscopic analysis of the relationship between cell organelles can be carried out with thick sections. The imidazole/osmium can be used routinely because the technical steps are easy and simple to follow. Furthermore, it can complement other cytochemical methods.

scholarly journals Histopathological evaluation of endometrial sampling in perimenopausal women with abnormal uterine bleeding

2021 ◽  
Vol 10 (8) ◽  
pp. 3180
Author(s):  
Bhavani L. Nair ◽  
Lency S. Kuriakose
Keyword(s):  
Endometrial Cancer ◽  
Abnormal Uterine Bleeding ◽  
Uterine Bleeding ◽  
Menstrual Bleeding ◽  
Endometrial Sampling ◽  
Medical College ◽  
Perimenopausal Women ◽  
Proliferative Endometrium ◽  
Secretory Endometrium

Background: Abnormal uterine bleeding (AUB) is one of the common symptoms in the gynaecology outpatient department. About one third of women are affected at some time in their lives. The perimenopausal women show significant number of underlying organic pathology. The evaluation of endometrium and/or organ histopathology has the dual advantage of finding the cause of AUB and to rule out endometrial cancer or the potential for cancer in future like endometrial hyperplasia with atypia. The aim of the study was to determine the histopathological pattern of endometrial sampling in perimenopausal women with AUB and to follow them up for a period of six months after the procedure.Methods: The prospective observational study was conducted at the department of obstetrics and gynaecology at Sree Gokulam Medical College and Research Foundation, Venjaramood, Thiruvananthapuram, Kerala, for a period of one year from December 2019 to December 2020. The study was conducted on 116 perimenopausal women 41-52 years who presented with AUB and had undergone endometrial sampling. These ladies were subsequently followed up for six months post procedure to assess the response to medical treatment or the need for any surgical intervention like hysterectomy.Results: A total 39.65% patients had heavy and prolonged menstrual bleeding and 18.16% patients had irregular bleeding. 14.65% patients had prolonged flow, 8.6% had heavy flow, 6.8% had infrequent with prolonged flow, 6% had prolonged, infrequent with heavy bleeding. Non-structural (COEIN) causes contributed to about 60.4% of AUB in perimenopausal women and 39.6% had structural (PALM) causes. 49% cases were secretory endometrium. 29.3% had disordered proliferative endometrium, 4.3% had proliferative endometrium, 5.2% each had polyp or hyperplasia without atypia. 18 (15.5%) cases underwent hysterectomy, 3 patients who had adenocarcinoma underwent staging laparotomy, 2 patients had LNG IUS insertion and 40 patients were on follow up requiring either no treatment and 53 (45.68%) patients were given antifibrinolytics or hormonal therapy.Conclusions: Heavy and prolonged menstrual bleeding was the most common presenting symptom. COEIN contributed to about 60.4% of cases. Evaluation of the endometrium showed that, secretory endometrium was commonest (49%) followed by disordered proliferative endometrium (29.3%). On follow up for six months, 15.5% patients underwent hysterectomy, 2 patients had insertion of levonorgestrel IUD, 45.68% patients had medical management with antifibrinolytics or hormones and were on follow up. The responsibility of gynaecologist in the management of AUB in perimenopausal women is to exclude hyperplasia of endometrium and endometrial cancer.

Plasma membrane-associate filament systems in cultured cells visualized by dry-cleaving

1983 ◽  
Vol 64 (1) ◽  
pp. 351-364
Author(s):  
D.A. Mesland ◽  
H. Spiele
Keyword(s):  
Cultured Cells ◽  
Hepatoma Cell ◽  
Cell Types ◽  
Membrane Skeleton ◽  
Adhesive Tape ◽  
Cell Organelles ◽  
Red Cell Membrane ◽  
Transmission Electron ◽  
General Network ◽  
Dry Cleaving

Substrate-attached critical-point-dried cells cleave along the level of the substrate-adherent membrane if removed by means of adhesive tape. The remaining membrane fragments on grids can be visualized three-dimensionally by means of stereo transmission electron microscopy. Attachment of cells may be achieved by active spreading of the cell, or artificially by poly-L-lysine adherence of prefixed cells. In 11 different cell types a filamentous network appears to remain associated with the cytoplasmic face of the membrane. In one hepatoma cell type virtually no filamentous network could be detected. Two general network morphologies are described: the hepatocytic network and the lymphoid network. Since no correspondence could be found between cytoplasmic structure and the structure of the membrane-associated network, and since cells generally cleave along the level of this network, excluding cell organelles, we conclude that it comprises a distinct structural system, analogous to the membrane skeleton of the red cell membrane.

scholarly journals Inhibition of Estrogenic Response of Yeast Screen Assay by Exposure to Non-Lethal Levels of Metallic Nanoparticles

2020 ◽  
Vol 10 (11) ◽  
pp. 3796
Author(s):  
Byoung-cheun Lee ◽  
Cuong N. Duong ◽  
Jungkon Kim ◽  
Suejin Kim ◽  
Ig-chun Eom ◽  
...  
Keyword(s):  
Metallic Nanoparticles ◽  
Estrogenic Activity ◽  
Endocrine Disrupting Compounds ◽  
Cell Organelles ◽  
Yeast Saccharomyces Cerevisiae ◽  
Tem Analysis ◽  
Environmental Media ◽  
Transmission Electron ◽  
17Β Estradiol

In order to investigate the effects of metallic nanoparticles (NPs) on the performance of in vitro bioassay, zinc oxide NP (ZnO NP), aluminum oxide NP (Al2O3 NP), bare silver NP (Ag NP), and Ag NP capped with citrate (Agcit NP) were evaluated with yeast (Saccharomyces cerevisiae Y190) two-hybrid system (YES assay), carrying Japanese medaka estrogen receptors (mERs) in the presence of 17β-estradiol (E2, 10−6 M), a reference chemical for estrogenic activity. The distribution of NPs in the yeast was also examined by field-emission transmission electron microscopy (FE-TEM). The results show that TEM analysis revealed that NPs were present inside the yeast and accumulated deep inside the cell organelles, suggesting that cell death was caused by NPs. However, despite no significant change of mortality, the E2 estrogenic activities in yeast exposed to ZnO NP and Al2O3 NP were dose-dependently reduced. For Ag NP and Agcit NP, such phenomenon observed in the exposure of ZnO NP and Al2O3 NP did not occur. From the observations, we found that ZnO NP and Al2O3 NP in the environmental media could result in underestimated estrogenicity of endocrine-disrupting compounds when evaluated by YES assay.

Survival and ultrastructure of gene-microinjected rabbit embryos after vitrification

Zygote ◽  
2005 ◽  
Vol 13 (4) ◽  
pp. 283-293 ◽  
Author(s):  
M. Popelková ◽  
P. Chrenek ◽  
J. Pivko ◽  
A.V. Makarevich ◽  
E. Kubovičová ◽  
...  
Keyword(s):  
Ultrastructural Level ◽  
Blastocyst Stage ◽  
Embryo Morphology ◽  
Hatching Rate ◽  
Transmission Electron ◽  
Cellular Debris ◽  
Or Gene ◽  
Rabbit Gene

Morphological signs of injury and subsequent regeneration following vitrification of either rabbit gene microinjected (Gene-Mi) or intact in vitro cultured embryos derived from in vivo fertilized eggs were evaluated by post-warming recovery in culture and analysed by transmission electron microscopy (TEM). The percentages of vitrified/warmed Gene-Mi embryos that reached the blastocyst stage (69%) and hatched (57%) did not differ significantly from those of intact embryos (78% and 56%, respectively). In contrast, in vitro development of embryos to the blastocyst stage among non-vitrified intact (96%) and Gene-Mi (90%) embryos compared with both the intact vitrified (78%) and Gene-Mi vitrified (69%) groups, as well as hatching rate (94%, 90% vs 56%, 57%, respectively) varied significantly (p<0.001). Observations by TEM showed that the vitrified/warmed intact or Gene-Mi embryos without post-culture displayed severe degenerative changes among their cells. During 24 h of culture a proportion of the embryos were able to regenerate and complete the compaction process. Nevertheless the signs of previous injury were retained, such as swollen cytoplasmic organelles and remaining cellular debris in the perivitelline space. These observations indicate that the procedure of gene Mi does not siginificantly compromise embryo tolerance to cryopreservation and post-warming developmental ability. Severe changes in embryo morphology, observed at the ultrastructural level, can be attributed to a direct influence of the vitrification process rather than to the Mi procedure itself.

Interrelations between the Parasitophorous Vacuole ofToxoplasma gondiiand Host Cell Organelles

2005 ◽  
Vol 11 (2) ◽  
pp. 166-174 ◽  
Author(s):  
Rodrigo Cardoso Magno ◽  
Lorian Cobra Straker ◽  
Wanderley de Souza ◽  
Marcia Attias
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Endoplasmic Reticulum ◽  
Host Cell ◽  
Fluorescent Probes ◽  
Parasitophorous Vacuole ◽  
Laser Scanning Microscopy ◽  
Cell Organelles ◽  
Apical Side ◽  
Transmission Electron

Toxoplasma gondii, the causative agent of toxoplasmosis, is capable of actively penetrating and multiplying in any nucleated cell of warm-blooded animals. Its survival strategies include escape from fusion of the parasitophorous vacuole with host cell lysosomes and rearrangement of host cell organelles in relation to the parasitophorous vacuole. In this article we report the rearrangement of host cell organelles and elements of the cytoskeleton of LLCMK2 cells, a lineage derived from green monkey kidney epithelial cells, in response to infection byT. gondiitachyzoites. Transmission electron microscopy made on flat embedded monolayers cut horizontally to the apical side of the cells or field emission scanning electron microscopy of monolayers scraped with scotch tape before sputtering showed that association of mitochondria to the vacuole is much less frequent than previously described. On the other hand, all parasitophorous vacuoles were surrounded by elements of the endoplasmic reticulum. These data were complemented by observations by laser scanning microscopy using fluorescent probes from mitochondria and endoplasmic reticulum and reinforced by three-dimensional reconstruction from serial sections observed by transmission electron microscopy and labeling of mitochondria and endoplasmic reticulum by fluorescent probes.

Fine structure of ocelli of the larval black fly Simulium vittatum (Diptera: Simuliidae)

1987 ◽  
Vol 65 (1) ◽  
pp. 142-150 ◽  
Author(s):  
Joyce M. Nyhof ◽  
Susan B. McIver
Keyword(s):  
Fine Structure ◽  
Polarized Light ◽  
Cell Organelles ◽  
Golgi Bodies ◽  
Simulium Vittatum ◽  
Pigment Granules ◽  
Transmission Electron ◽  
Structural Differences ◽  
Numerous Cell ◽  
Retinular Cells

The fine structure of light- and dark-adapted ocelli of last instar larval Simulium vittatum Zetterstedt was described using scanning and transmission electron microscopy. Larvae have six ocelli arranged in groups of three on each side of the head. The larger two ocelli of each group are externally visible as two darkly pigmented eyespots. The third, smaller ocellus lacks pigmentation and, therefore, is not externally visible. Each ocellus has its long axis oriented dorso-ventrally, has 13 retinular cells, and lacks an expanded cuticular lens. Conspicuous rhabdoms occur in the three ocelli. The rhabdoms of the pigmented ocelli are centrally located and enveloped by pigment granules. The microvilli of the rhabdoms are oriented primarily in one plane, an indication of a possible sensitivity to polarized light. The rhabdom of the unpigmented ocellus is eccentrically located and its microvilli are not uniplanar. Each ocellus has numerous cell organelles, including mitochondria, ribosomes, endoplasmic reticulum, and Golgi bodies. Especially conspicuous are membranous figures, which are associated with the nuclei and vary in size and complexity from simple stacks to lamellar whorls. These latter organelles are probably involved in the turnover processes of the rhabdomeric membranes. In light- and dark-adapted ocelli the only structural differences were associated with the microvilli and multivesicular bodies. Differences in location of pigment granules and in size of rhabdomeres and membranous figures were not observed.

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