Interleukin 1 induction of a serine esterase in a murine T cell line is inhibited by fetal calf serum

1989 ◽  
Vol 20 (1) ◽  
pp. 35-39 ◽  
Author(s):  
Susanna E. Schlegel-Haueter ◽  
Florence Aebischer
Keyword(s):  
T Cell ◽  
Cell Line ◽  
Fetal Calf Serum ◽  
Interleukin 1 ◽  
Calf Serum ◽  
Serine Esterase

Fish kidney cell line in response to heat shock

1992 ◽  
Vol 50 (1) ◽  
pp. 704-705
Author(s):  
Li-Chu Tung ◽  
Yung-Reui Chen ◽  
Shiu-Nan Chen ◽  
Guang-Hsiung Kuo
Keyword(s):  
Heat Shock ◽  
Cell Line ◽  
Fetal Calf Serum ◽  
Calf Serum ◽  
Uranyl Acetate ◽  
Ultrastructural Changes ◽  
Heat Shock Treatment ◽  
Acanthopagrus Schlegeli ◽  
Cacodylate Buffer ◽  
Fish Kidney

In the present study, the ultrastructural changes of BPK cells, a fibroblast-like cell line, derived from the kidney of juvenile black porgy Acanthopagrus schlegeli, under heat shock treatment are described.The BPK cells were maintained in L-15 medium supplemented with 10% fetal calf serum and 0.15 M NaCl at 28|C2. The heating was carried out in precalibrated water baths. Monolayers of cells, grown on coverslips in parafilm-sealed petri dishes were submerged under water for 30 min at 40|C treatments. Cells were fixed in 2.5% glutaraldehyde in 0.1 M cacodylate buffer supplemented with 6.6% sucrose, postfixed in 1% OsO4 and flat embedded in Spurr’s resin. Silver section were cut parallel to the substratum, stained with uranyl acetate and Reynold’s lead citrate, and examined in a Hitachi H-600 electron microscope at 75 KV.

scholarly journals High-titer lentiviral vectors stimulate fetal calf serum-specific human CD4 T-cell responses: implications in human gene therapy

Gene Therapy ◽  
2009 ◽  
Vol 16 (6) ◽  
pp. 788-795 ◽  
Author(s):  
L Bao ◽  
H Guo ◽  
X Huang ◽  
S Tammana ◽  
M Wong ◽  
...  
Keyword(s):  
Gene Therapy ◽  
T Cell ◽  
Fetal Calf Serum ◽  
Human Gene ◽  
High Titer ◽  
Calf Serum ◽  
T Cell Responses ◽  
Cd4 T Cell ◽  
Human Gene Therapy ◽  
Cell Responses

scholarly journals Activity and interleukin 1 responsiveness of SV40 enhancer motifs in a rodent immature T cell line.

1990 ◽  
Vol 9 (3) ◽  
pp. 929-937 ◽  
Author(s):  
E. Espel ◽  
C. Fromental ◽  
P. Reichenbach ◽  
M. Nabholz
Keyword(s):  
T Cell ◽  
Cell Line ◽  
Interleukin 1 ◽  
Sv40 Enhancer

Immune Response to Fetal Calf Serum by Two Adenosine Deaminase-Deficient Patients After T Cell Gene Therapy

2002 ◽  
Vol 13 (13) ◽  
pp. 1605-1610 ◽  
Author(s):  
Laura Tuschong ◽  
Sherry L. Soenen ◽  
R. Michael Blaese ◽  
Fabio Candotti ◽  
Linda Mesler Muul
Keyword(s):  
Gene Therapy ◽  
Immune Response ◽  
T Cell ◽  
Adenosine Deaminase ◽  
Fetal Calf Serum ◽  
Calf Serum ◽  
Cell Gene

scholarly journals Helper signals in the plaque-forming cell response to protein-bound haptens.

1983 ◽  
Vol 158 (2) ◽  
pp. 317-333 ◽  
Author(s):  
N W Roehm ◽  
P Marrack ◽  
J W Kappler
Keyword(s):  
T Cell ◽  
B Cell ◽  
Cell Line ◽  
Interleukin 1 ◽  
Interleukin 2 ◽  
Tumor Cell Line ◽  
Con A ◽  
B Cell Growth Factor ◽  
Nonspecific Factors ◽  
Culture Supernatants

We have demonstrated the ability of a series of murine T cell hybridomas to deliver an antigen-specific, B cell I-region-restricted helper signal in the generation of specific PFC responses to protein-bound haptens. With some hybridomas the elicitation of optimal PFC responses required the addition of nonspecific factors provided by culture supernatants of concanavalin A-stimulated (Con A SN) spleen cells. Using hapten-primed B cells depleted of both T cells and macrophages (Mphi) we have now demonstrated a requirement for three nonspecific factor preparations to substitute for spleen Con A SN in the elicitation of optimal PFC responses. The first preparation was the interleukin 1 containing culture supernatant of the Mphi tumor cell line P388D1, the second the interleukin 2 (IL-2) and B cell growth factor containing Con A SN of the T cell hybridoma FS6-14.13, and the third, the gamma interferon containing Con A SN of the T cell hybridoma FS7-20.6.18. The P388D1 and FS6-14.13 factor preparations were most effective when added at the initiation of culture, while the FS7-20.6.18 factor preparation was most effective when added at 24 h of culture. The activity of FS6-14.13 Con A SN was depleted by incubation with the IL-2-dependent T cell line HT-2. The activity of FS7-20.6.18 Con A SN was abrogated by incubation at pH 2. The results suggest that the generation of PFC responses to protein-bound haptens require at least three nonspecific factors in addition to an antigen/Ia specific helper signal.

scholarly journals Modulation of in vitro eosinophil progenitors by hydrocortisone: role of accessory cells and interleukins

Blood ◽  
1985 ◽  
Vol 66 (5) ◽  
pp. 1072-1079
Author(s):  
FT Slovick ◽  
CN Abboud ◽  
JK Brennan ◽  
MA Lichtman
Keyword(s):  
T Cell ◽  
Cell Line ◽  
Conditioned Medium ◽  
Mononuclear Cells ◽  
Interleukin 1 ◽  
Interleukin 2 ◽  
Accessory Cell ◽  
Direct Function ◽  
Human Eosinophil ◽  
Accessory Cells

The growth of human eosinophil progenitors (CFU-Eo) and the modulation of growth by hydrocortisone were studied as functions of the presence of lymphocytes and monocytes in marrow cells under study; and the source of colony-stimulating factors, specifically, media conditioned by macrophage-like cell line, GCT; phytohemagglutinin-stimulated mononuclear cells (PHA-LCM); or the T cell line, MO. CFU-Eo growth was greatest in marrow containing accessory cells as compared to marrow depleted of accessory cells; and in marrow treated with phytohemagglutinin-stimulated leukocyte conditioned media (PHA-LCM) or MO (T cell line)-conditioned medium (MO-CM) as compared with GCT cell- conditioned medium (GCT-CM). Hydrocortisone reproducibly inhibited eosinophil progenitor growth in unfractionated marrow stimulated by GCT- CM. This effect was abrogated by admixing irradiated mononuclear cells or T lymphocytes with the target marrow or by adding interleukin 1 or interleukin 2 (IL-1, IL-2). Inhibition by hydrocortisone did not occur when monocyte and T lymphocyte depleted marrow was studied. Unlike GCT- CM, MO-CM and PHA-LCM stimulated equal proportions of eosinophil progenitors in nondepleted and accessory cell-depleted marrow and demonstrated less hydrocortisone inhibition. However, both GCT-CM and PHA-LCM produced in the presence of hydrocortisone stimulated significantly fewer CFU-Eos in both unfractionated and accessory cell- depleted marrow target populations. These results indicate that the growth of CFU-Eo and inhibition of growth by hydrocortisone is a direct function of a monocyte-T cell interaction and probably is mediated through effects on the production/release of eosinophil colony stimulating factor (Eo-CSF).

Sign in / Sign up

Export Citation Format

Share Document