scholarly journals Serological and virological evidence of non-sexual transmission of human herpesvirus type 8 (HHV8)

2000 ◽  
Vol 125 (3) ◽  
pp. 671-675 ◽  
Author(s):  
F. VITALE ◽  
E. VIVIANO ◽  
A. M. PERNA ◽  
F. BONURA ◽  
G. MAZZOLA ◽  
...  
Keyword(s):  
General Population ◽  
Dna Sequences ◽  
Mononuclear Cells ◽  
Sexual Transmission ◽  
Human Herpesvirus ◽  
Seroprevalence Rate ◽  
Nested Polymerase Chain Reaction ◽  
Peripheral Blood Mononuclear ◽  
Herpesvirus Type ◽  
Human Herpesvirus Type

To evaluate whether or not human herpesvirus 8 (HHV8) can be transmitted through a non-sexual route a serological survey was carried out in a group of 51 catholic nuns. The seroprevalence rate and the geometrical mean antibody titre to anti-latent HHV8 antigen were similar in nuns and in a group of 60 women, matched by age, in the general population (27 vs. 24%; 1028 vs. 1575, respectively). Moreover, by using nested polymerase chain reaction (PCR), HHV8 DNA sequences were detected in 7 of 16 (43·8%) saliva and peripheral blood mononuclear cells (PBMC) from patients with classical Kaposi's sarcoma (KS) and in 3 out of 7 (42%) AIDS-KS patients. None of 5 HIV positive persons who did not have KS tested positive for HHV8 DNA. HHV8 DNA sequences were also detected in 2 of 12 (17%) saliva and 1 PBMC sample out of 12 healthy HHV8 positive individuals (age range: 30–80 years old). This paper suggests that non-sexual transmission of HHV8 is operating in our geographical setting and saliva may be a potential source of HHV8 spreading in the general population.

scholarly journals Human Herpesvirus Type 8 DNA Sequences in Cell-Free Plasma and Mononuclear Cells of Kaposi's Sarcoma Patients

1996 ◽  
Vol 174 (5) ◽  
pp. 1101-1104 ◽  
Author(s):  
W. J. Harrington ◽  
O. Bagasra ◽  
C. E. Sosa ◽  
L. E. Bobroski ◽  
M. Baum ◽  
...  
Keyword(s):  
Dna Sequences ◽  
Kaposi's Sarcoma ◽  
Mononuclear Cells ◽  
Human Herpesvirus ◽  
Kaposi’S Sarcoma ◽  
Herpesvirus Type ◽  
Human Herpesvirus Type ◽  
Free Plasma

Human herpesvirus type 8 DNA sequences in biological samples of HIV-positive and negative individuals in Sicily

AIDS ◽  
1997 ◽  
Vol 11 (5) ◽  
pp. 607-612 ◽  
Author(s):  
Enza Viviano ◽  
Francesco Vitale ◽  
Francesca Ajello ◽  
Anna Maria Perna ◽  
Maria Rosaria Villafrate ◽  
...  
Keyword(s):  
Dna Sequences ◽  
Biological Samples ◽  
Human Herpesvirus ◽  
Hiv Positive ◽  
Herpesvirus Type ◽  
Human Herpesvirus Type

Clinical Features and Viral Excretion in An Infant with Primary Human Herpesvirus 7 Infection

PEDIATRICS ◽  
1995 ◽  
Vol 95 (2) ◽  
pp. 187-190
Author(s):  
Yoshizo Asano ◽  
Sadao Suga ◽  
Tetsushi Yoshikawa ◽  
Takehiko Yazaki ◽  
Takashi Uchikawa
Keyword(s):  
Clinical Features ◽  
Dna Sequences ◽  
Mononuclear Cells ◽  
Human Herpesvirus ◽  
Cervical Lymphadenopathy ◽  
Prior History ◽  
Antibody Activity ◽  
Nested Polymerase Chain Reaction ◽  
Viral Excretion ◽  
Convalescent Stage

Objective. To find clinical features of a virologically-confirmed patient with primary human herpesvirus 7 (HHV-7) infection and a relationship of the excretion of viruses between HHV-7 and human herpesvirus 6 (HHV-6). Patient and Methods. A 13-month-old boy who had a known prior history of exanthem subitum at 6 months of age developed fever for 3 days and a skin rash appeared as the fever was resolving. The course was accompanying with nonspecific signs and symptoms such as anorexia, irritability, mild diarrhea, palpebral edema, mild inflammation of pharynx, and mild occipital and cervical lymphadenopathy. Heparinized blood samples were used for isolation of HHV-6 and HHV-7 and detection of both virus DNA sequences by a nested polymerase chain reaction (PCR) amplification. Samples from other body sites were also tested for their DNA sequences using the PCR. Both virus antibody activity was measured by an indirect immunofluorescent assay or a neutralization test. Results. Cultured mononuclear cells from the patient at the acute stage of the disease produced morphologic changes, which reacted only with the monoclonal antibody to HHV-7 but not with the antibody to HHV-6. Both viruses were not isolated from blood obtained at the convalescent stage. An antibody response of the patient indicated a seroconversion to HHV-7 but not to other microbial agents including HHV-6 and Mycoplasma pneumoniae. Both virus DNA sequences were detected in peripheral blood mononuclear cells at acute and convalescent stages. HHV-7 DNA was excreted into saliva and transiently into stool at an early convalescent stage followed by HHV-6 excretion into saliva. No HHV-7 and HHV-6 was excreted into urine. Conclusions. Clinical features of a virologically confirmed patient with primary HHV-7 infection were comparable with those of primary HHV-6 infection and HHV-7 infection may reactivate HHV-6.

scholarly journals The Kaposi sarcoma-associated herpesvirus (KSHV) is present as an intact latent genome in KS tissue but replicates in the peripheral blood mononuclear cells of KS patients.

1996 ◽  
Vol 184 (1) ◽  
pp. 283-288 ◽  
Author(s):  
L L Decker ◽  
P Shankar ◽  
G Khan ◽  
R B Freeman ◽  
B J Dezube ◽  
...  
Keyword(s):  
Dna Sequences ◽  
Peripheral Blood ◽  
Viral Genome ◽  
Mononuclear Cells ◽  
Human Herpesvirus ◽  
Kaposi Sarcoma ◽  
Body Cavity ◽  
Apparent Size ◽  
Peripheral Blood Mononuclear ◽  
Barr Virus

Short DNA sequences have been identified, originally in association with Kaposi's sarcoma (KS) biopsies, that are highly homologous to oncogenic, lymphotropic herpesviruses. Recently a virus, Kaposi sarcoma associated herpesvirus (KSHV) or human herpesvirus-8 (HHV-8), bearing these sequences has been identified in a cell line derived from a body cavity-based lymphoma. In this report, we show that the same sequences are present in KS biopsies as DNA molecules of a form and size characteristic of latent herpesviruses-large, covalently closed, circular episomes. The genomes migrate with an apparent size larger than the herpesvirus Epstein-Barr virus (172 kb). This form of the viral genome was found in four of four biopsies and three of five peripheral blood samples from KS patients. Linear forms of the viral genome, characteristic of viral replication, were not detected in the biopsies, but were present in the peripheral blood of three out of five patients. The sequences for KSHV/HHV-8 were also detected in the blood of four of five allograft patients and three of five healthy donors without KS suggesting that the virus is widespread throughout the human population.

scholarly journals Seroprevalence of Human Herpesvirus Type 2 in a Reference Center for Pregnant Women in Rio de Janeiro, Brazil

2017 ◽  
Vol 22 ◽  
pp. 20 ◽  
Author(s):  
Lyana Lima ◽  
Guilherme Padalecki ◽  
Carlos Castro ◽  
Jânio Cordeiro ◽  
Vanessa De Paula
Keyword(s):  
Pregnant Woman ◽  
Pregnant Women ◽  
Primary Infection ◽  
Care Center ◽  
Sexual Transmission ◽  
Human Herpesvirus ◽  
Herpesvirus Type ◽  
High Prevalence ◽  
Human Herpesvirus Type ◽  
Relevant Group

Introduction: Pregnant women stand as an relevant group for research about Human Herpesvirus (HHV-2) infection owing to the risk of mother-to-child transmission. Methods: Women attending in a prenatal care center were tested for HHV-2 IgM and IgG by ELISA. Quantitative PCR test was the chosen method to ascertain viremia. Results: The seroprevalence of IgG and IgM anti-HHV-2 was 20.6% and 2.2% respectively. HHV-2   viremia was found in one pregnant  woman with HHV-2 IgM, leading to the assumption of primary infection.  Conclusion: The significantly high prevalence of HHV-2 found and the ascertainment of primary infection in a pregnant woman underline the need for constant HHV-2 follow-up and diagnosis in order to avoid sexual transmission.

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