Emergence of group B Streptococcus serotype IV in women of child-bearing age in Ireland

2010 ◽  
Vol 139 (2) ◽  
pp. 236-238 ◽  
Author(s):  
R. A. KIELY ◽  
L. COTTER ◽  
A. M. MOLLAGHAN ◽  
B. CRYAN ◽  
A. COFFEY ◽  
...  
Keyword(s):  
Genetic Heterogeneity ◽  
Dna Analysis ◽  
Random Amplified Polymorphic Dna ◽  
Group B Streptococcus ◽  
Serotype Distribution ◽  
Carriage Rate ◽  
Child Bearing ◽  
High Prevalence ◽  
Vaginal Swabs ◽  
Group B

SUMMARYThis study determined the carriage rate and serotype distribution of group B Streptococcus (GBS) in women of child-bearing age in the southern region of Ireland. A total of 2000 vaginal swabs collected in two periods in 2004 and 2006 were examined and revealed a GBS carriage rate of 16·1%. Serotyping of isolates showed that serotypes Ia, II, III, IV, and V were the most prevalent. A high prevalence of serotype IV was found, increasing from 7·6% to 15·2% between 2004 and 2006. Random amplified polymorphic DNA analysis demonstrated considerable genetic heterogeneity in the serotype IV isolates. This serotype should be considered for inclusion in potential vaccines for use in Ireland.

scholarly journals Genomic and phenotypic characterisation of invasive neonatal and colonising group B Streptococcus isolates from Slovenia, 2001–2018

2020 ◽  
Vol 20 (1) ◽  
Author(s):  
Tina Perme ◽  
Daniel Golparian ◽  
Maja Bombek Ihan ◽  
Andrej Rojnik ◽  
Miha Lučovnik ◽  
...  
Keyword(s):  
Pregnant Women ◽  
Virulence Factors ◽  
Late Onset ◽  
Group B Streptococcus ◽  
Genomic Diversity ◽  
Neonatal Disease ◽  
Phenotypic Characterisation ◽  
High Prevalence ◽  
Group B ◽  
The Impact

Abstract Background Group B Streptococcus (GBS) is the leading cause of invasive neonatal disease in the industrialized world. We aimed to genomically and phenotypically characterise invasive GBS isolates in Slovenia from 2001 to 2018 and contemporary colonising GBS isolates from screening cultures in 2018. Methods GBS isolates from 101 patients (invasive isolates) and 70 pregnant women (colonising isolates) were analysed. Basic clinical characteristics of the patients were collected from medical records. Antimicrobial susceptibility and phenotypic capsular serotype were determined. Whole-genome sequencing was performed to assign multilocus sequence types (STs), clonal complexes (CCs), pathogenicity/virulence factors, including capsular genotypes, and genome-based phylogeny. Results Among invasive neonatal disease patients, 42.6% (n = 43) were females, 41.5% (n = 39/94) were from preterm deliveries (< 37 weeks gestation), and 41.6% (n = 42) had early-onset disease (EOD). All isolates were susceptible to benzylpenicillin with low minimum inhibitory concentrations (MICs; ≤0.125 mg/L). Overall, 7 serotypes were identified (Ia, Ib, II-V and VIII); serotype III being the most prevalent (59.6%). Twenty-eight MLST STs were detected that clustered into 6 CCs. CC-17 was the most common CC overall (53.2%), as well as among invasive (67.3%) and non-invasive (32.9%) isolates (p < 0.001). CC-17 was more common among patients with late-onset disease (LOD) (81.4%) compared to EOD (47.6%) (p < 0.001). The prevalence of other CCs was 12.9% (CC-23), 11.1% (CC-12), 10.5% (CC-1), 8.2% (CC-19), and 1.8% (CC-498). Of all isolates, 2.3% were singletons. Conclusions A high prevalence of hypervirulent CC-17 isolates, with low genomic diversity and characteristic profile of pathogenicity/virulence factors, was detected among invasive neonatal and colonising GBS isolates from pregnant women in Slovenia. This is the first genomic characterisation of GBS isolates in Slovenia and provides valuable microbiological and genomic baseline data regarding the invasive and colonising GBS population nationally. Continuous genomic surveillance of GBS infections is crucial to analyse the impact of IND prevention strategies on the population structure of GBS locally, nationally, and internationally.

scholarly journals Genital tract group B streptococcal colonization in pregnant women: a South Indian perspective

2011 ◽  
Vol 5 (08) ◽  
pp. 592-595 ◽  
Author(s):  
Vijayan Sharmila ◽  
Noyal Mariya Joseph ◽  
Thirunavukkarasu Arun Babu ◽  
Latha Chaturvedula ◽  
Sujatha Sistla
Keyword(s):  
Pregnant Women ◽  
Group B Streptococcus ◽  
Colonization Rate ◽  
Antibiotic Susceptibility Pattern ◽  
South Indian ◽  
Vaginal Swabs ◽  
Rectal Swabs ◽  
Resistance To Penicillin ◽  
Group B ◽  
A Prospective Study

Introduction: During the last few decades, group B Streptococcus (GBS) has emerged as an important pathogen. The major reservoirs for GBS are the vagina and the peri-anal regions/rectum, and the colonization of these regions is a risk factor for subsequent infection in pregnant women and newborns. Methodology: A prospective study was performed to determine the prevalence of GBS colonization in the vagina and rectum of pregnant women and the antibiotic susceptibility pattern of the isolates. We also aimed to identify risk factors associated with GBS colonization. The vaginal and rectal swabs were inoculated in Todd-Hewitt broth and later subcultured on blood agar for isolation of GBS. Results: A total of 300 pregnant women were enrolled in the study. GBS strains were isolated from seven out of 300 patients, corresponding to a colonization rate of 2.3%. Of the seven patients carrying GBS, isolates were cultured only from vaginal swabs in two cases (28.6%), only from rectal swabs in two cases (28.6%) from both vaginal and rectal swabs in three cases (42.9%). Heavy colonization was present only in 42.9% (3/7) of antenatal women. None of the seven isolates were resistant to penicillin or clindamycin, while one isolate (14.3%) was resistant to erythromycin and five isolates (71.4%) were resistant to tetracycline. Multigravid women and those with previous spontaneous abortion were more frequently colonized by GBS. Conclusion: The GBS colonization rate in our study was low. No resistance to penicillin or clindamycin was seen, while the majority of the isolates were resistant to tetracycline.

scholarly journals Group B Streptococcus serotypes associated with different clinical syndromes: Asymptomatic carriage in pregnant women, intrauterine fetal death, and early onset disease in the newborn

PLoS ONE ◽  
2020 ◽  
Vol 15 (12) ◽  
pp. e0244450
Author(s):  
Yulia Schindler ◽  
Galia Rahav ◽  
Israel Nissan ◽  
Liora Madar-Shapiro ◽  
Julia Abtibol ◽  
...  
Keyword(s):  
Pregnant Women ◽  
Early Onset ◽  
Fetal Death ◽  
Group B Streptococcus ◽  
Intrauterine Fetal Death ◽  
Carriage Rate ◽  
Asymptomatic Carriage ◽  
Clinical Syndromes ◽  
Group B ◽  
Dominant Serotype

Objectives To study Group B Streptococcus (GBS) isolates associated with different clinical syndromes: asymptomatic carriage in pregnant women, intrauterine fetal death (IUFD), and early onset disease (EOD) in the newborn. Methods GBS isolates were collected from asymptomatic pregnant women admitted for labor, IUFD cases, and neonates with EOD. Serotypes and antibiotic susceptibilities were determined. Multilocus sequence typing (MLST) was performed to assess genetic epidemiology. Results GBS carriage rate was 26.1% (280/1074). The dominant serotype among asymptomatic pregnant women was VI [98/240 women (40.8%)], followed by serotypes III, V and IV in 42/240 (17.5%), 30/240 (12.5%) and 28/240 (11.7%) women, respectively. The dominant serotype in IUFD cases was serotype VI [10/13 (76.9%)]. In contrast the prevalent serotype among EOD cases was III [16/19 (84.2%)]. ST-1 was associated with IUFD [7/13 (53.8%)], ST-17 was associated with serotype III and EOD in the newborn 14/19 (73.7%)]. Erythromycin and clindamycin resistance reached 36.8%, 7.7% and 20.0%among EOD, vaginal carriage and IUFD, respectively. Conclusions Serotypes VI and ST-1 were dominant among asymptomatic pregnant women and in IUFD cases while EOD was associated with serotype III and ST-17. Invasive mechanisms thus may differ between IUFD and EOD in the newborn and virulence may be related to capsule serotype. Resistance rates to erythromycin and clindamycin were high in EOD cases.

scholarly journals A composite transposon associated with erythromycin and clindamycin resistance in group B Streptococcus

2007 ◽  
Vol 56 (7) ◽  
pp. 947-955 ◽  
Author(s):  
Karen M. Puopolo ◽  
David C. Klinzing ◽  
Michelle P. Lin ◽  
Derek L. Yesucevitz ◽  
Michael J. Cieslewicz
Keyword(s):  
Genetic Basis ◽  
Southern Hybridization ◽  
Rapd Analysis ◽  
Random Amplified Polymorphic Dna ◽  
Group B Streptococcus ◽  
Macrolide Resistance ◽  
Chromosomal Site ◽  
Streptococcal Species ◽  
Group B ◽  
Us Cities

Group B Streptococcus (GBS) resistant to erythromycin and clindamycin has been isolated with increasing frequency since the mid-1990s. This work studied GBS isolates from three US cities to determine the genetic basis of the macrolide resistance phenotype. ermB genes were amplified from five isolates collected in Boston, Pittsburgh and Seattle from infant and adult sources. Gene-walking methods were used to determine the chromosomal location of ermB and to identify associated genes. Southern mapping and random amplified polymorphic DNA (RAPD) analyses were used to distinguish the isolates. The ermB gene was present on the chromosome within a composite Tn917/Tn916-like transposon similar to one identified in Streptococcus pneumoniae. Four strains from Boston and Pittsburgh were serotype V and identical by Southern hybridization and RAPD analysis. The Seattle isolate was serotype Ib, with different patterns on RAPD analysis and Southern mapping. The composite transposon was integrated at an identical chromosomal site in all five isolates. The presence of this composite transposon in both GBS and pneumococci suggests that ermB-mediated macrolide resistance in streptococci may be due to the horizontal transfer of a mobile transposable element, and raises concern for further dissemination of high-grade erythromycin and clindamycin resistance among streptococcal species.

scholarly journals Group B streptococcus colonization, antibiotic susceptibility, and serotype distribution among Saudi pregnant women

2020 ◽  
Vol 52 (1) ◽  
pp. 70
Author(s):  
Amr Mohamed Mohamed ◽  
Mubashir Ahmad Khan ◽  
Aftab Faiz ◽  
Jawwad Ahmad ◽  
Elsheikh Babiker Khidir ◽  
...  
Keyword(s):  
Pregnant Women ◽  
Antibiotic Susceptibility ◽  
Group B Streptococcus ◽  
Serotype Distribution ◽  
Group B
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