Trypanosoma cruzi genotyping supports a common source of infection in a school-related oral outbreak of acute Chagas disease in Venezuela

2013 ◽  
Vol 142 (1) ◽  
pp. 156-162 ◽  
Author(s):  
Z. DÍAZ-BELLO ◽  
M. C. THOMAS ◽  
M. C. LÓPEZ ◽  
R. ZAVALA-JASPE ◽  
O. NOYA ◽  
...  
Keyword(s):  
Trypanosoma Cruzi ◽  
Chagas Disease ◽  
Clinical Manifestations ◽  
Northern Region ◽  
Common Source ◽  
Endemic Region ◽  
Spliced Leader ◽  
Source Of Infection ◽  
Human Infections ◽  
Acute Chagas Disease

SUMMARYTrypanosoma cruzi I, a discrete typing unit (DTU) found in human infections in Venezuela and other countries of the northern region of South America and in Central America, has been recently classified into five intra-DTU genotypes (Ia, Ib, Ic, Id, Ie) based on sequence polymorphisms found in the spliced leader intergenic region. In this paper we report the genotype identification of T. cruzi human isolates from one outbreak of acute orally acquired Chagas disease that occurred in a non-endemic region of Venezuela and from T. cruzi triatomine and rat isolates captured at a guava juice preparation site which was identified as the presumptive source of infection. The genotyping of all these isolates as TcId supports the view of a common source of infection in this oral Chagas disease outbreak through the ingestion of guava juice. Implications for clinical manifestations and dynamics of transmission cycles are discussed.

scholarly journals Simultaneous identification of Trypanosoma cruzi surface and internal antigens reactive to different immunoglobulin classes (radio-immunoblotting)

1990 ◽  
Vol 32 (5) ◽  
pp. 379-383 ◽  
Author(s):  
Anna Maria Simonsen Stolf ◽  
Eufrosina Setsu Umezawa ◽  
Bianca Zingales
Keyword(s):  
Trypanosoma Cruzi ◽  
Western Blot ◽  
Chagas Disease ◽  
Western Blotting ◽  
Specific Antibodies ◽  
Internal Parasite ◽  
Sds Page ◽  
Blotting Technique ◽  
Simultaneous Identification ◽  
Acute Chagas Disease

A radioactive Western-blotting technique was developed by which the reactivity of Immunoglobulins (Igs) from different classes to both membrane radiolabelled and internal parasite antigens is simultaneously identified. The method includes radioiodination of parasites, polypeptide fractionation by SDS-PAGE, Western-blot transfer and autoradiography of the immunoblots developed with anti-Igs conjugates labelled with enzymes. The analysis is then performed by the comparison of common bands on the autoradiograms and the respective substrate stained nitrocellulose blots. This technique was used to analyse T. cruzi trypomastigote surface labelled antigens reactive to IgM, IgA and IgG specific antibodies. A different pattern of reactivity with acute Chagas' disease patients sera was thus obtained.

scholarly journals Fatal acute Chagas disease by Trypanosoma cruzi DTU TcI, Ecuador

2020 ◽  
Vol 20 (1) ◽  
Author(s):  
Manuel Calvopina ◽  
Gabriela Segovia ◽  
William Cevallos ◽  
Yosselin Vicuña ◽  
Jaime A. Costales ◽  
...  
Keyword(s):  
Trypanosoma Cruzi ◽  
Chagas Disease ◽  
Acute Chagas Disease

scholarly journals Trypanosoma cruzi infection associated with atypical clinical manifestation during the acute phase of the Chagas disease

2019 ◽  
Vol 12 (1) ◽  
Author(s):  
Lucia Rangel-Gamboa ◽  
Lirio López-García ◽  
Francisco Moreno-Sánchez ◽  
Irma Hoyo-Ulloa ◽  
María Elisa Vega-Mémije ◽  
...  
Keyword(s):  
Trypanosoma Cruzi ◽  
Chagas Disease ◽  
Acute Phase ◽  
Clinical Manifestations ◽  
Chronic Phase ◽  
Clinical History ◽  
Skin Lesions ◽  
Small Subunit ◽  
Serological Tests ◽  
Cutaneous Manifestations

Abstract Background Chagas disease (CD) is caused by the protozoan parasite Trypanosoma cruzi and is transmitted by triatomine insects. Clinical manifestations vary according to the phase of the disease. Cutaneous manifestations are usually observed in the acute phase (chagoma and Romaña’s sign) or after reactivation of the chronic phase by immunosuppression; however, a disseminated infection in the acute phase without immunosuppression has not been reported for CD. Here, we report an unusual case of disseminated cutaneous infection during the acute phase of CD in a Mexican woman. Methods Evaluation of the patient included a complete clinical history, a physical exam, and an exhaustive evaluation by laboratory tests, including ELISA, Western blot and PCR. Results Skin biopsies of a 50-year-old female revealed intracellular parasites affecting the lower extremities with lymphangitic spread in both legs. The PCR tests evaluated biopsy samples obtained from the lesions and blood samples, which showed a positive diagnosis for T. cruzi. Partial sequencing of the small subunit ribosomal DNA correlated with the genetic variant DTU II; however, serological tests were negative. Conclusions We present a case of CD with disseminated skin lesions that was detected by PCR and showed negative serological results. In Mexico, an endemic CD area, there are no records of this type of manifestation, which demonstrates the ability of the parasite to initiate and maintain infections in atypical tissues.

scholarly journals Treg Cells Induced by rSSP4 Derived fromT. cruziAmastigotes Increase Parasitemia in an Experimental Chagas Disease Model

2013 ◽  
Vol 2013 ◽  
pp. 1-10 ◽  
Author(s):  
Y. Flores-García ◽  
J. L. Rosales-Encina ◽  
V. H. Rosales-García ◽  
A. R. Satoskar ◽  
P. Talamás-Rohana
Keyword(s):  
T Cells ◽  
Trypanosoma Cruzi ◽  
Chagas Disease ◽  
Disease Model ◽  
Treg Cells ◽  
Immunosuppressive Activity ◽  
Positive Role ◽  
Cardiac Inflammation ◽  
Considerable Controversy ◽  
Acute Chagas Disease

Currently, there is a considerable controversy over the participation of Treg cells duringTrypanosoma cruziinfection, the main point being whether these cells play a negative or a positive role. In this work, we found that the adoptive transfer of CD4+CD25+FOXP3+T cells from rSSP4- (a recombinantTrypanosoma cruziamastigote derived protein, previously shown to have immunomodulatory properties on macrophages) immunized BALB/c donors into syngenic recipients simultaneously withT. cruzichallenge reduces cardiac inflammation and prolongs hosts’ survival but increases blood parasitemia and parasite loads in the heart. These CD4+CD25+FOXP3+Treg cells from immunized mice have a relatively TGF-β-dependent suppressive activity on CD4+T cells. Therefore, regulatory CD4+CD25+T cells play a positive role in the development of acuteT. cruziinfection by inducing immunosuppressive activity that controls early cardiac inflammation during acute Chagas disease, prolonging survival, but at the same time promoting parasite growth.

BloodstreamTrypanosoma cruziparasites from mice simultaneously express antigens that are markers of acute and chronic human Chagas disease

Parasitology ◽  
1991 ◽  
Vol 102 (3) ◽  
pp. 379-385 ◽  
Author(s):  
M. S. Leguizamon ◽  
O. E. Campetella ◽  
M. B. Reyes ◽  
C. F. Ibañez ◽  
M. A. Basombrio ◽  
...  
Keyword(s):  
Trypanosoma Cruzi ◽  
Chagas Disease ◽  
Acute Phase ◽  
Early Period ◽  
Chronic Phase ◽  
Blood Parasites ◽  
Expression Library ◽  
Antibody Specificities ◽  
Human Infections ◽  
Parasite Strains

Several recombinantTrypanosoma cruziproteins previously isolated were used as antigens to analyse antibody specificities present in sera from human infections. Some parasite proteins such as SAPA (Shed Acute Phase Antigen) are antigenic early after infection. Others, like antigens 1 and 30, are antigenic mainly during the chronic phase of the infection. To understand why different proteins are antigenic at different periods of infection, specificities of antibodies present in the sera of infected mice were compared with the antigens expressed by parasites collected directly from blood. Parasites collected during the acute parasitaemia peak expressed not only antigen SAPA, but also antigens 1 and 30. However, only antibodies against SAPA were frequently observed during the early period and also in the chronic phase of murine infection. Long-lasting antibodies against SAPA were detected regardless of the mouse and parasite strains used. Furthermore, all 8 recombinant clones detected in aT. cruziexpression library with pooled sera from acutely infected mice were homologous to the SAPA gene. These results show that even though parasites from the acute parasitaemia peak in mice may express simultaneously several proteins known to be antigenic, only antibodies against SAPA were consistently detected.

scholarly journals A ascendência da doença de Chagas aguda como uma doença veiculada por alimentos na região Norte do Brasil / The ancestry of acute Chagas disease as a foodborne illness in the northern region of Brazil

2021 ◽  
Vol 7 (12) ◽  
pp. 117507-117524
Author(s):  
Laisy Nazaré Araújo Da Cunha ◽  
Rodrigo Pereira Pamplona Rodrigues ◽  
Francisco das Chagas Alves Do Nascimento ◽  
Andrea das Graças Ferreira Frazão ◽  
Ana Lúcia da Silva Rezende ◽  
...  
Keyword(s):  
Chagas Disease ◽  
Northern Region ◽  
Foodborne Illness ◽  
Acute Chagas Disease

scholarly journals NATURAL INFECTION BY Trypanosoma cruzi IN ONE DOG IN CENTRAL WESTERN BRAZIL: A CASE REPORT

2013 ◽  
Vol 55 (4) ◽  
pp. 287-289 ◽  
Author(s):  
Arleana do Bom Parto Ferreira de Almeida ◽  
Daphine Ariadne Jesus de Paula ◽  
Maria Luisa Paro Otton ◽  
Felipe Wolf Jaune ◽  
Raquel Aparecida Sales da Cruz ◽  
...  
Keyword(s):  
Trypanosoma Cruzi ◽  
Chagas Disease ◽  
Natural Infection ◽  
Human Populations ◽  
Direct Examination ◽  
Chain Reaction ◽  
Mato Grosso ◽  
Blood Smears ◽  
Polymerase Chain ◽  
Acute Chagas Disease

SUMMARY It is estimated that about 10 million people are infected with Trypanosoma cruzi worldwide, mostly in Latin America and more than 25 million are at risk of acquiring this infection in endemic areas. Dogs are an important reservoir for this pathogen and thus, considered a risk factor for human populations. This report describes one case of Chagas disease in a dog from Cuiabá, Mato Grosso State, Brazil. The diagnosis was obtained by direct examination of trypomastigote forms in blood smears. Amastigotes forms were visualized in microscopy of the bone marrow, lymph nodes, kidneys, liver and brain. The T. cruzi (ZIII) infection was confirmed by Polymerase Chain Reaction, and sequencing. The animal presented multisystemic failure and died. Although acute Chagas disease in humans is not reported in Cuiabá, this is the first report of a canine case in this region. This case represents a warning, to health professionals and authorities, to the possibility of transmission of this zoonosis in Cuiabá.

scholarly journals Disagreement between PCR and serological diagnosis of Trypanosoma cruzi infection in blood donors from a Colombian endemic region

Biomédica ◽  
2021 ◽  
Vol 41 (Supl. 1) ◽  
pp. 47-59
Author(s):  
Liliana Torcoroma García Sánchez ◽  
Jhancy Rocío Aguilar Jiménez ◽  
Marly Yojhana Bueno ◽  
Erika Marcela Moreno Moreno ◽  
Herminia Ramírez ◽  
...  
Keyword(s):  
Trypanosoma Cruzi ◽  
Chagas Disease ◽  
Blood Donors ◽  
False Negative ◽  
Chronic Phase ◽  
Antibody Detection ◽  
Low Grade ◽  
Endemic Region ◽  
Infected People ◽  
Cruzi Infection

Introduction: Chagas’ disease is the leading cause of infectious myocarditis worldwide. This infection caused by Trypanosoma cruzi is usually life-long and asymptomatic; however, the third part of infected people can develop severe or even fatal cardiomyopathy. As the parasitemia in the chronic phase is both low-grade and intermittent, T. cruzi infection is principally detected by serology, although this method has sensitivity and specificity limitations.Objective: To determine the level of agreement between serologic and molecular tests in 658 voluntary blood donors from six provinces in the Colombian department of Santander.Materials and methods: We evaluated an array of diagnostic technologies by cross-section sampling performing a serological double diagnostic test for T. cruzi antibody detection (Chagas III ELISA™, BiosChile Group, and ARCHITECT Chagas CMIA™, Abbott), and DNA detection by polymerase chain reaction (PCR). We collected the demographic, clinical, and epidemiological information of participants. The sample size was calculated using Epidat™ and the statistical analysis was done with Stata 12.1™.Results: PCR was six times more sensitive in detecting T. cruzi infection than ELISA/CMIA with prevalence values of 1.8% (12/658) and 0.3% (2/658), respectively, and kappa=0.28 (95%CI: -0.03 - 0.59). In contrast, serology showed a sensitivity of 16.7% (95%CI: 2.09 - 48.4) and a specificity of 100% (95%CI: 99.4 - 100). All seropositive samples were found to be positive by PCR.Conclusions: The implementation of PCR as a complementary method for screening donors could reduce the probability of false negative and the consequent risk of transfusional-transmission of Chagas’ disease, especially in endemic regions.

scholarly journals Trypanosoma cruzi defined antigens in the serological evaluation of an outbreak of acute Chagas disease in Brazil (Catolé do Rocha, Paraíba)

1996 ◽  
Vol 91 (1) ◽  
pp. 87-93 ◽  
Author(s):  
Eufrosina S Umezawa ◽  
Maria Aparecida Shikanai-Yasuda ◽  
Arthur Gruber ◽  
Vera L Pereira-Chioccola ◽  
Bianca Zingales
Keyword(s):  
Trypanosoma Cruzi ◽  
Chagas Disease ◽  
Acute Chagas Disease
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