Serotype and surface protein gene distribution of colonizing group B streptococcus in women in Egypt

2013 ◽  
Vol 142 (1) ◽  
pp. 208-210 ◽  
Author(s):  
S. SHABAYEK ◽  
S. ABDALLA ◽  
A. MH. ABOUZEID
Keyword(s):  
High Frequency ◽  
Protein Gene ◽  
Group B Streptococcus ◽  
Surface Protein ◽  
Surface Proteins ◽  
Protein Encoding ◽  
Vaginal Swabs ◽  
Surface Protein Gene ◽  
Group B ◽  
Encoding Genes

SUMMARYGroup B streptococcus (GBS) is a leading cause of neonatal sepsis and meningitis. We determined the distribution of serotypes and surface protein encoding genes of GBS strains from pregnant and non-pregnant women in Egypt. Vaginal swabs from 364 women were screened by culture and 100 (27·4%) yielded GBS. Serotype V was the most predominant (33%), followed by serotypes II (17%), III (15%), Ia (14%), VI (12%), Ib (8%) and IV (1%). The most common surface protein genes were epsilon (27%), alp3 (26%), bca (18%), rib (16%) and alp2 (10%). Two isolates were negative for surface protein genes. The distribution of serotypes and surface proteins was similar to reports from other parts of the world but the relatively high frequency of serotype VI was a notable feature of the strains from women in Egypt.

Reproducible and variable rearrangements of a Tetrahymena thermophila surface protein gene family occur during macronuclear development

1988 ◽  
Vol 8 (11) ◽  
pp. 5043-5046
Author(s):  
J P Kile ◽  
H D Love ◽  
C A Hubach ◽  
G A Bannon
Keyword(s):  
Environmental Regulation ◽  
Cdna Clone ◽  
Multigene Family ◽  
Protein Gene ◽  
Tetrahymena Thermophila ◽  
Surface Protein ◽  
Surface Proteins ◽  
Hybridization Probe ◽  
Macronuclear Development ◽  
Surface Protein Gene

The expression of Tetrahymena surface proteins serotype H3 (SerH3) and serotype T (SerT) is under environmental regulation. SerH3 is expressed when cells are incubated between the temperatures of 20 and 35 degrees C, while SerT is expressed when cells are grown at temperatures above 35 degrees C. Using a SerH3 cDNA clone as a hybridization probe, we determined that (i) the SerH3 gene is a member of a multigene family; (ii) most members of this multigene family are variably rearranged during macronuclear development; and (iii) the gene which produces the SerH3 mRNA is reproducibly rearranged during macronuclear development.

scholarly journals Reproducible and variable rearrangements of a Tetrahymena thermophila surface protein gene family occur during macronuclear development.

1988 ◽  
Vol 8 (11) ◽  
pp. 5043-5046 ◽  
Author(s):  
J P Kile ◽  
H D Love ◽  
C A Hubach ◽  
G A Bannon
Keyword(s):  
Environmental Regulation ◽  
Cdna Clone ◽  
Multigene Family ◽  
Protein Gene ◽  
Tetrahymena Thermophila ◽  
Surface Protein ◽  
Surface Proteins ◽  
Hybridization Probe ◽  
Macronuclear Development ◽  
Surface Protein Gene

The expression of Tetrahymena surface proteins serotype H3 (SerH3) and serotype T (SerT) is under environmental regulation. SerH3 is expressed when cells are incubated between the temperatures of 20 and 35 degrees C, while SerT is expressed when cells are grown at temperatures above 35 degrees C. Using a SerH3 cDNA clone as a hybridization probe, we determined that (i) the SerH3 gene is a member of a multigene family; (ii) most members of this multigene family are variably rearranged during macronuclear development; and (iii) the gene which produces the SerH3 mRNA is reproducibly rearranged during macronuclear development.

scholarly journals Group B Streptococcal Surface Proteins as Targets for Protective Antibodies: Identification of Two Novel Proteins in Strains of Serotype V

1999 ◽  
Vol 67 (12) ◽  
pp. 6350-6357 ◽  
Author(s):  
Thomas Areschoug ◽  
Margaretha Stålhammar-Carlemalm ◽  
Charlotte Larsson ◽  
Gunnar Lindahl
Keyword(s):  
Vaccine Development ◽  
Group B Streptococcus ◽  
Surface Protein ◽  
Cross Reactivity ◽  
Active Immunization ◽  
Surface Proteins ◽  
Protective Antibodies ◽  
Group B ◽  
Different Strains ◽  
Type V

ABSTRACT Strains of group B streptococcus (GBS) express surface proteins that confer protective immunity. In particular, most strains of the four classical capsular serotypes (Ia, Ib, II, and III) express either of the Rib and α proteins, two members of the same protein family. Here, we report a study of surface proteins expressed by strains of serotype V, which has recently emerged as an important serotype among GBS strains causing serious disease. Two novel GBS proteins were identified, purified, and characterized. One of these proteins, designated Fbs, was immunologically unrelated to other GBS surface proteins. This ∼110-kDa protein was found in 15 of 49 (31%) type V isolates but in few strains of other serotypes. The Fbs proteins expressed by different strains showed limited variation in size. The most common surface protein among type V strains, found in 29 of 49 (59%) isolates, was designated Rib-like, since it cross-reacted with Rib but was not immunologically identical to Rib. Characterization of this Rib-like protein showed that the N-terminal sequence (12 residues) was identical to that of α, although these two proteins lacked cross-reactivity. The biochemical and immunological properties of the Rib-like GBS protein indicate that it is closely related to the R28 protein of Streptococcus pyogenes. Importantly, passive and active immunization experiments with mice showed that the Fbs and Rib-like proteins are targets for protective antibodies. These two proteins are therefore of interest for analysis of pathogenic mechanisms and for vaccine development.

scholarly journals High prevalence of group B streptococcus ST17 hypervirulent clone among non-pregnant patients from a Hungarian venereology clinic

2019 ◽  
Vol 19 (1) ◽  
Author(s):  
Szilvia Kardos ◽  
Adrienn Tóthpál ◽  
Krisztina Laub ◽  
Katalin Kristóf ◽  
Eszter Ostorházi ◽  
...  
Keyword(s):  
Genetic Relatedness ◽  
Clinical Symptoms ◽  
Strong Association ◽  
Group B Streptococcus ◽  
Surface Protein ◽  
Surface Proteins ◽  
High Prevalence ◽  
Intrapartum Antibiotic ◽  
Group B ◽  
Open Question

Abstract Background Although Streptococcus agalactiae is the leading causative agent of neonatal sepsis and meningitis, recently it is increasingly isolated from non-pregnant adults. The relation between its presence in the genitourinary tract and manifested clinical symptoms of STD patients remains an open question. In this study, a complex epidemiological investigation of GBS isolates from a venerology clinic was performed. Methods Ninety-six GBS isolates were serotyped and their genetic relatedness determined by PFGE. MLST was also performed for a subset of 20 isolates. The antibiotic susceptibility was tested with agar dilution. Surface proteins and the ST-17 hypervirulent clone was detected by PCR. Results The serotype prevalence was the following: V (29.2%), III (27.1%), Ia (22.9%), IV (10.4%), II (5.2%) and Ib (4.2%). A strong association was demonstrated between surface protein genes and serotypes. All isolates were fully susceptible to penicillin, but erythromycin and clindamycin resistance was high (41.7 and 35.4%, respectively), and 8 phenotypically macrolide sensitive isolates carried the ermB gene. 21.9% of all strains belonged to the hypervirulent ST17 clone, most being of serotype III and all were rib +. We found a few serotype IV isolates belonging to several STs and one serotype V/ST110 strain, containing a 44-bp deletion in the atr allele. Conclusions The presence of silent ermB genes is of worry, as their expression upon macrolide exposure could lead to unforeseen therapeutic failure, while clindamycin is used for intrapartum antibiotic prophylaxis, in case of penicillin allergy. The other alarming result is the high prevalence of ST17 among these strains from STD patients, who could be sources of further infections. This is the first report from Hungary providing both serotyping and genotyping data of GBS isolates. These results could be helpful for vaccine production as the major vaccine candidates are capsular antigens or surface proteins.

scholarly journals Genetic characterization and diversity of Streptococcus agalactiae isolates with macrolide resistance

2010 ◽  
Vol 59 (7) ◽  
pp. 780-786 ◽  
Author(s):  
Monika Brzychczy-Włoch ◽  
Tomasz Gosiewski ◽  
Małgorzata Bodaszewska ◽  
Wojciech Pabian ◽  
Małgorzata Bulanda ◽  
...  
Keyword(s):  
Streptococcus Agalactiae ◽  
Group B Streptococcus ◽  
Surface Protein ◽  
Macrolide Resistance ◽  
Surface Proteins ◽  
Erythromycin Resistance ◽  
High Genetic Diversity ◽  
Genes Encoding ◽  
Resistant Strains ◽  
Group B

Macrolide resistance in 169 Streptococcus agalactiae [group B streptococcus (GBS)] isolates originating from pregnant carriers was investigated. Using multiplex PCR the presence of genes encoding erythromycin resistance and capsular polysaccharides, as well as surface proteins, was determined. Random amplification of polymorphic DNA (RAPD) and PFGE were used to characterize specific clones among the isolates. In the examined population of women, erythromycin-resistant strains were found in 4.5 % of patients, whereas clindamycin-resistant strains were found in 3 % of patients, which was 16 % of strains resistant to erythromycin and 10 % of strains resistant to clindamycin among GBS isolates, respectively. Among the isolates, the largest percentage was represented by the constitutive macrolide–lincosamide–streptogramin B (cMLSB) phenotype (63 %), then the inductive macrolide–lincosamide–streptogramin B (iMLSB) phenotype (26 %) and the macrolide resistance (M) phenotype (11 %). The ermB gene was indicated in all isolates with the cMLSB phenotype and V serotype, whereas mefA/mefE genes were found in isolates with the M phenotype and Ia serotype. Among resistance isolates, serotype V was predominant (67 %), followed by serotypes II (15 %), Ia (11 %) and III (7 %). The most common surface protein encoding genes were alp3 (70 %), then rib (11 %), epsilon (7.5 %), bca (7.5 %) and alp2 (4 %). A statistically significant relationship between macrolide resistance, serotype V and the alp3 gene was demonstrated. PFGE, in comparison to the RAPD method, gave better genetic discrimination of GBS isolates. A relatively high genetic diversity among investigated strains was shown. In addition, the largest genetic homogeneity was found in serotype V.

scholarly journals Identification of novel cps locus polymorphisms in nontypable group B Streptococcus

2006 ◽  
Vol 55 (6) ◽  
pp. 775-783 ◽  
Author(s):  
Srinivas V. Ramaswamy ◽  
Patricia Ferrieri ◽  
Lawrence C. Madoff ◽  
Aurea E. Flores ◽  
Nikhil Kumar ◽  
...  
Keyword(s):  
Capsular Polysaccharide ◽  
Group B Streptococcus ◽  
Surface Protein ◽  
The Elderly ◽  
Surface Proteins ◽  
Pcr Analysis ◽  
Genetic Profile ◽  
Gene Encoding ◽  
Group B ◽  
Type V

Group B Streptococcus (GBS) is an important pathogen responsible for a variety of diseases in newborns and the elderly. A clinical GBS isolate is considered nontypable (NT) when serological methods fail to identify it as one of nine known GBS serotypes. Eight clinical isolates (designated A1–A4, B1–B4) showed PFGE profiles similar to that of a GBS serotype V strain expressing R1, R4 surface proteins. These unique isolates were further characterized by immunologic and genetic methods. Rabbit sera to isolates A1 and A2 reacted weakly with concentrated HCl extracts of A1–A4 isolates, but not with those of B1–B4 isolates. In addition, a type V capsular polysaccharide (CPS) inhibition ELISA revealed that cell wall extracts from isolates A1–A4, but not from B1–B4, expressed low but measurable amounts of type V CPS. Molecular serotyping with PCR analysis showed that all eight isolates contained a type V-specific CPS gene (cpsO) and harboured the gene encoding the surface protein Alp3. Multilocus sequence typing identified isolate A1 as belonging to a new sequence type (ST) designated ST-173, whereas the other seven isolates keyed to ST-1. Sequencing of the 18 genes (17 736 bp) in the cps locus showed that each NT isolate harboured one to three unique polymorphisms, and also identified an IS1381 element in cpsE of the B4 isolate. Collectively, genetic and immunologic analyses revealed that these NT isolates expressing R1, R4 proteins have a genetic profile consistent with that of type V, an emergent, antigenically diverse and increasingly prevalent GBS serotype.

scholarly journals CRISPR activation screen in mice identifies novel membrane proteins enhancing pulmonary metastatic colonisation

2021 ◽  
Vol 4 (1) ◽  
Author(s):  
Louise van der Weyden ◽  
Victoria Harle ◽  
Gemma Turner ◽  
Victoria Offord ◽  
Vivek Iyer ◽  
...  
Keyword(s):  
Cell Surface ◽  
Surface Protein ◽  
Surface Proteins ◽  
Guide Rnas ◽  
Protein Encoding ◽  
Target Molecules ◽  
Novel Target ◽  
Encoding Genes ◽  
Crispr Activation

AbstractMelanoma represents ~5% of all cutaneous malignancies, yet accounts for the majority of skin cancer deaths due to its propensity to metastasise. To develop new therapies, novel target molecules must to be identified and the accessibility of cell surface proteins makes them attractive targets. Using CRISPR activation technology, we screened a library of guide RNAs targeting membrane protein-encoding genes to identify cell surface molecules whose upregulation enhances the metastatic pulmonary colonisation capabilities of tumour cells in vivo. We show that upregulated expression of the cell surface protein LRRN4CL led to increased pulmonary metastases in mice. Critically, LRRN4CL expression was elevated in melanoma patient samples, with high expression levels correlating with decreased survival. Collectively, our findings uncover an unappreciated role for LRRN4CL in the outcome of melanoma patients and identifies a potential therapeutic target and biomarker.

scholarly journals Performance of PhenoMatrix for the detection of Group B Streptococcus from recto-vaginal swabs

2021 ◽  
pp. 115427
Author(s):  
Claudio Foschi ◽  
Gabriele Turello ◽  
Tiziana Lazzarotto ◽  
Simone Ambretti
Keyword(s):  
Group B Streptococcus ◽  
Vaginal Swabs ◽  
Group B

Three strains of Wolbachia pipientis and high rates of infection in Iranian sandfly species

2014 ◽  
Vol 104 (2) ◽  
pp. 195-202 ◽  
Author(s):  
A. Bordbar ◽  
S. Soleimani ◽  
F. Fardid ◽  
M.R. Zolfaghari ◽  
P. Parvizi
Keyword(s):  
Protein Gene ◽  
Surface Protein ◽  
Male And Female ◽  
Wolbachia Pipientis ◽  
Major Surface Protein ◽  
Zoonotic Cutaneous Leishmaniasis ◽  
Geographical Regions ◽  
Surface Protein Gene ◽  
Major Surface ◽  
Zoonotic Visceral Leishmaniasis

AbstractIndividual wild-caught sandflies from Iran were examined for infections of Wolbachia pipientis by targeting the major surface protein gene wsp of this intracellular α-proteobacterium. In total, 638 male and female sandflies were screened, of which 241 were found to be positive for one of three wsp haplotypes. Regardless of geographical origins and habitats, Phlebotomus (Phlebotomus) papatasi and other sandfly species were found to be infected with one common, widespread strain of A-group W. pipientis (Turk 54, GenBank accession EU780683; AY288297). In addition, a new A-group haplotype (Turk07, GenBank accession KC576916) was isolated from Phlebotomus (Paraphlebotomus) mongolensis and Phlebotomus (Pa.) caucasicus, and a new B-group haplotype (AZ2331, GenBank accession JX488735) was isolated from Phlebotomus (Larroussius) perfiliewi. Therefore, Wolbachia was found to occur in at least three of the incriminated vectors of zoonotic cutaneous leishmaniasis and zoonotic visceral leishmaniasis in different geographical regions of Iran. It may provide a new tool for the future control of leishmaniasis.

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