Novel Second Generation Platinum Containing Antineoplastic Agents Ssp, Sap, and Poly-Plat and Their Effect on Glucose 6 Phosphate Dehydrogenase (Ec 1.1.1.49) in the Liver and Kidney of Male Wistar Rats.

1997 ◽  
Vol 3 (S2) ◽  
pp. 57-58
Author(s):  
T. P. Multhaupt ◽  
S.K. Aggarwal
Keyword(s):  
Antineoplastic Agents ◽  
Wistar Rats ◽  
Second Generation ◽  
Normal Kidney ◽  
Male Wistar Rats ◽  
Liver And Kidney ◽  
New Compounds ◽  
Glucose 6 Phosphate Dehydrogenase ◽  
Liver Tissues

Poly-(trans-l,2-diaminocyclohexane) platinumj-carboxyamylose (Poly-Plat); 5-SuIfosalicylato-trans-(l,2-diaminocyclohexane) platinum (SSP); and 4-Hydroxy-a-sulfonylphenylacetato (trans 1,2-diaminocyclohexane) platinum (II) (SAP) (Andrulis Pharmaceuticals, Bethesda, MD) are three novel second generation platinum containing antineoplastic compounds. Initial studies indicate that these agents are more effective in the treatment of cancer while at the same time less toxic to the organism as a whole than cisplatin (CDDP). The present study was undertaken to examine the effects of these new compounds on glucose-6-phosphate dehydrogenase (G-6-PDH) as compared to CDDP treated and normal kidney and liver tissues.Wistar rats (100-120g) were given intraperitoneal injections of CDDP (9 mg/ kg) and Poly-Plat, SSP and SAP (10 mg/ kg) over a 5 day period. On day 6 the animals were sacrificed and tissues (kidney and liver) were freeze sectioned (7 μm). Sections were incubated in media according to the accepted method specific for the G-6-PDH localization at a pH of 7.46 for 30 min.

scholarly journals Microscopic and biochemical changes on liver and kidney of Wistar rats on combination antiretroviral therapy: the impact of naringenin and quercetin

2020 ◽  
Vol 9 (5) ◽  
pp. 601-608
Author(s):  
Edidiong Nnamso Akang ◽  
Olufunke O Dosumu ◽  
Ini-ibehe Essien Okoko ◽  
Oluwatomisin Faniyan ◽  
Ademola A Oremosu ◽  
...  
Keyword(s):  
Antiretroviral Therapy ◽  
Wistar Rats ◽  
Combination Antiretroviral Therapy ◽  
Necrosis Factor Alpha ◽  
Immunodeficiency Virus ◽  
Male Wistar Rats ◽  
Liver And Kidney ◽  
Factor Alpha ◽  
Continuous Use ◽  
The Impact

Abstract Combination antiretroviral therapy (cART), which is a lifelong therapy for people living with human immunodeficiency virus, has been associated with nephrotoxicity and hepatotoxicity leading to its discontinuation. This study aimed at investigating the ameliorative potential of naringenin and quercetin on cART-induced hepatotoxicity and nephrotoxicity. Seventy male Wistar rats (225–260 g) were divided into seven groups as control, cART, naringenin, quercetin, dimethyl sulfoxide (DMSO), naringenin/cART (CN) and quercetin/cART (CQ). cART (24 mg/kg), naringenin (50 mg/kg) and quercetin (50 mg/kg) were dissolved in 1% v/v DMSO and administered orally for 56 days. Combination of cART and bioflavonoids had significant increase in superoxide dismutase (P < 0.05), catalase (P < 0.01), reduced glutathione (P < 0.001) and decreased malondialdehyde (P < 0.001) compared to cART only. Tumor necrosis factor Alpha (TNFα) level increased significantly in cART and CQ (P < 0.01) groups, while others showed no significant changes compared to control. TNFα also significantly decreased in CQ level compared to cART (P < 0.001). In addition, significant increase in creatinine level in cART only indicated progressive renal toxicity. Also, progressive pathological changes including congested blood vessels and hepatocellular necrosis were found in the liver, while the kidney had glomerular atrophy, and tubular distortion in cART-only group. Control, naringenin- and quercetin-treated groups showed normal renal and hepatic cytoarchitecture. These findings elucidate that progressive renal and hepatic toxicity is associated with the continuous use of cART; however, a combination of quercetin and naringenin with cART showed possible potential of ameliorating the damages posed by cART.

scholarly journals Comparisons of Curative Effects of Chlorophyll fromSauropus androgynus(L) Merr Leaf Extract and Cu-Chlorophyllin on Sodium Nitrate-Induced Oxidative Stress in Rats

2016 ◽  
Vol 2016 ◽  
pp. 1-7 ◽  
Author(s):  
Suparmi Suparmi ◽  
Minidian Fasitasari ◽  
Martanto Martosupono ◽  
Jubhar Christian Mangimbulude
Keyword(s):  
Oxidative Stress ◽  
Sodium Nitrate ◽  
Wistar Rats ◽  
Histological Evaluation ◽  
Female Wistar Rats ◽  
Male Wistar Rats ◽  
Liver And Kidney ◽  
Serum Biochemical ◽  
Hb Concentration ◽  
Rbc Count

Sodium nitrate (NaNO2) widely used as food additive for coloring and preserving meat has been reported to induce oxidative stress and cause histopathologic changes, nitrosative tissue damage, and lipid peroxidation in liver and kidney. Therefore, the present study compared the curative effect of chlorophyll fromSauropus androgynus(L) Merr and Cu-chlorophyllin as antioxidant in NaNO2-induced female Wistar rats based on haematological, serum biochemical, and histological evaluation. Thirty male Wistar rats were randomly assigned into six groups of five rats each. NaNO2were given at a subacute dose of 50 mg/kg bw intraperitoneally for 10 days. Chlorophyll fromS. androgynusand Cu-chlorophyllin from K-Liquid™ were given in the following 14 days at the two doses: 0,016 mg/mL and 0.008 mg/mL. NaNO2exposure resulted in significant reductions (p<0.05) in values of packed cell volume (PCV), haemoglobin (Hb) concentration and red blood cell (RBC) count, transferrin, and ferritin and elevation in malondialdehyde (MDA) level and schistocytes percentage with insignificant reductions in serum albumin and transferrin levels. Histology of kidney and liver were changed insignificantly (p>0.05) to normal values. Chlorophyll fromS. androgynusand Cu-chlorophyllin possess antioxidant potentials to protect against toxicities induced by sodium nitrate.

scholarly journals The Green Bitter Weed (Hymenoxys odorato) Plant Extract Is Toxic to the Liver and Kidney of Wistar Rats

2018 ◽  
Author(s):  
John Juma Ochieng ◽  
Isaac Echoru ◽  
Musa Ajibola Iyiola
Keyword(s):  
Body Weight ◽  
Wistar Rats ◽  
Complete Blood Count ◽  
Medical Personnel ◽  
Control Group ◽  
Dependent Manner ◽  
Snake Bites ◽  
Male Wistar Rats ◽  
Liver And Kidney ◽  
High Doses

Background: Medicinal plants are of great importance to health of individual and communities. About 80% of the population in Uganda relies on traditional medicine because western-trained medical personnel are limited especially in villages. Most Ugandans use Hymenoxys odorato for medicinal purposes e.g. to treat colds, fever, coughs, anti-helminthes, locally used as tea, anti-allergy and also as an anti-venom to relieve snake bites. Method: A group of 25 male wistar rats of 150 g&ndash;210 g were kept for 14 days while being fed and treated with the extract. At 14th day, anesthesia was given and blood samples collected by cardiac puncture for hematological and biochemical investigations. Serum was analyzed for Alkaline Phosphatase, Aspartate Transaminase and Alanine Transaminase while whole blood was used for complete blood count. The liver and kidney were removed and placed in 10% formalin to prepare for histology staining using haematoxylin and eosin technique. Results: The extract elevated hepatic biomarker enzymes i.e. ALP, ALT and AST. The increase was found to be significantly different (P &gt; 0.05) at 400 and 500 mg/kg doses as compared to the control group. Histological sections of the liver showed distortion of liver cytoarchitecture, steatosis, necrosis of hepatocytes and congestion of the sinusoids at high doses 300, 400 and 500 mg/kg body weight. In the sections of the kidney, there was mild distortion of the integrity of the kidney with glomerular hypercellularity at high doses (400 and 500 mg/kg per body weight). Conclusion: Hymenoxys odorato aqueous extract has toxic effects on the liver and kidney of wistar rats. The effects were observed to be in a dose dependent manner.

Toxicological evaluations of methanolic extract of Moringa oleifera leaves in liver and kidney of male Wistar rats

2013 ◽  
Vol 24 (4) ◽  
Author(s):  
Ademola A. Oyagbemi ◽  
Temitayo O. Omobowale ◽  
Ibraheem O. Azeez ◽  
John O. Abiola ◽  
Rahamon A.M. Adedokun ◽  
...  
Keyword(s):  
Wistar Rats ◽  
Moringa Oleifera ◽  
Methanolic Extract ◽  
Male Wistar Rats ◽  
Liver And Kidney ◽  
Moringa Oleifera Leaves
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