Background:
Moringa oleifera is currently and rapidly gaining superfood status.
Objective:
This study evaluated the effect of Moringa oleifera leaves (MOLM) on haematological,
biochemical and histopathology indices of rats fed with Vitamins and Minerals Deficient (VMD) diets.
Methods:
Twenty-five male Wistar rats (220-290g) were randomly assigned into 5 groups of 5 rats
each. The control (normal rat chow), remaining four diets were VMD plus MOLM at 0, 3, 5 and
10%. Haematological, biochemical and histopathological statuses were evaluated after 28 days.
Results:
Non-significant increase in RBC was observed in rats fed with the MOLM diets compared
to control. Rats on VMD diet had the lowest haemoglobin levels, whereas haemoglobin increased
with MOLM. No significant differences were observed in haematocrit, MCH, MCHC, platelets,
RDW and MPV in all the treatments and control group. However, VMD rats showed a decrease in
haemoglobin, and an increase in WBC and platelets. Electrolytes and glucose for all the treatments
and control were not significantly different, but, control and VMD10 diets rats showed significantly
higher values for creatinine compared to MOLM diets; total protein and albumin increased with
MOLM and were higher than in VMD and control rats. Serum activities of hepatic, cardiac and adrenal
marker enzymes were observed to be significantly lower in MOLM rats compared to VMD and
control. Mild congestion in VMD and VMD3 rats’ liver and diffused congestion in the kidney of
VMD diet rats were observed.
Conclusion:
The significantly high increase in platelet count in the VMD treated rats, indicative of
anemia and other physiological imbalances, highlights the effect of micronutrient (mineral and vitamins)
deficiencies in the diet. There is also the possibility that the anti-nutrients factors present in
Moringa oleifera prevented uptake and bioavailability of nutrients, since the MOLM did not undergo
any processing that could have removed or reduced these antinutrients. Further studies are still needed
on Moringa oleifera leaves to validate the bioavailability of nutrients from the plant.
Effects of aqueous extract of Moringa oleifera seed on cadmium-induced reproductive toxicity in male Wistar rats
