Morphological Aspects of the Traumatic Chronic Subdural Hematoma Capsule: SEM Studies

2007 ◽  
Vol 13 (3) ◽  
pp. 211-219 ◽  
Author(s):  
Marek Moskała ◽  
Igor Gościński ◽  
Józef Kałuża ◽  
Jarosław Polak ◽  
Mariusz Krupa ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Blood Vessels ◽  
Morphological Changes ◽  
Chronic Subdural Hematoma ◽  
Dynamic Changes ◽  
Inflammatory Reactions ◽  
Morphological Aspects ◽  
Thin Walled ◽  
Scanning Electron

The morphology of the outer and inner membranes of traumatic chronic subdural hematomas (CSDHs) surgically removed from eight patients was investigated by scanning electron microscopy (SEM). Hematomas were divided into three groups based on time that had passed from the initiation of trauma to surgery. Structure of the CSDHs showed gradual morphological changes of the developing hematoma capsule. They initially included angiogenic and aseptic inflammatory reactions followed by progressive involvement of fibroblasts—proliferating and producing collagen fibrils. Numerous capillaries suggesting formation of new blood vessels were observed mainly in young hematomas removed between 15 and 21 days after trauma. In “older” hematomas (40 days after trauma), more numerous capillaries and thin-walled sinusoids were accompanied by patent, larger diameter blood vessels. Within the fibrotic outer membrane of the “oldest” hematoma capsules (60 or more days after trauma), especially in the area over the hematoma cavity, blood vessels were frequently occluded by clots. The results suggest dynamic changes in cellular and vascular organization of traumatic CSDH capsules paralleling the progression in hematoma age.

scholarly journals Morphological aspects of marine monogeneans (Platyhelminthes) parasitic on the gills of Auxis thazard (Lacépède) (Scombridae) from Rio de Janeiro, Brazil

2004 ◽  
Vol 21 (2) ◽  
pp. 201-206 ◽  
Author(s):  
Cristina Dias Mogrovejo ◽  
Herman Lent ◽  
Cláudia Portes Santos
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Rio De Janeiro ◽  
Morphological Changes ◽  
Morphological Aspects ◽  
Auxis Thazard ◽  
Scanning Electron

Allopseudaxine macrova (Unnithan, 1957), Churavera triangula (Mamaev, 1967), Hexostoma auxisi Palombi, 1943 and H. keokeo Yamaguti, 1968 are studied based on light and scanning electron microscopy. The previously proposed synonymy of H. auxisi and H. keokeo is not accepted. Morphological changes between juvenile and adult hexostomatids are highlighted.

scholarly journals Three-dimensional relationships between tumor cells and microcirculation with double cyanine immunolabeling, laser scanning confocal microscopy, and computer-assisted reconstruction: an alternative to cast corrosion preparations.

1994 ◽  
Vol 42 (5) ◽  
pp. 681-686 ◽  
Author(s):  
V Rummelt ◽  
L M Gardner ◽  
R Folberg ◽  
S Beck ◽  
B Knosp ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Blood Vessels ◽  
Tumor Cells ◽  
Laser Scanning ◽  
Three Dimensional ◽  
Melanoma Cells ◽  
Tumor Blood Vessels ◽  
The Relationship ◽  
Scanning Electron

The morphology of the microcirculation of uveal melanomas is a reliable market of tumor progression. Scanning electron microscopy of cast corrosion preparations can generate three-dimensional views of these vascular patterns, but this technique sacrifices the tumor parenchyma. Formalin-fixed wet tissue sections 100-150 microns thick from uveal melanomas were stained with the lectin Ulex europaeus agglutinin I (UEAI) and proliferating cell nuclear antigen (PCNA) to demonstrate simultaneously the tumor blood vessels and proliferating tumor cells. Indocarbocyanine (Cy3) was used as a fluorophore for UEAI and indodicarbocyanine (Cy5) was used for PCNA. Double labeled sections were examined with a laser scanning confocal microscope. Images of both stains were digitized at the same 5-microns intervals and each of the two images per interval was combined digitally to form one image. These combined images were visualized through voxel processing to study the relationship between melanoma cells expressing PCNA and various microcirculatory patterns. This technique produces images comparable to scanning electron microscopy of cast corrosion preparations while permitting simultaneous localization of melanoma cells expressing PCNA. The microcirculatory tree can be viewed from any perspective and the relationship between tumor cells and the tumor blood vessels can be studied concurrently in three dimensions. This technique is an alternative to cast corrosion preparations.

Field-Emission Scanning Electron Microscopy and Energy-Dispersive X-Ray Analysis to Understand the Role of Tannin-Based Dyes in the Degradation of Historical Wool Textiles

2014 ◽  
Vol 20 (5) ◽  
pp. 1534-1543 ◽  
Author(s):  
Annalaura Restivo ◽  
Ilaria Degano ◽  
Erika Ribechini ◽  
Josefina Pérez-Arantegui ◽  
Maria Perla Colombini
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Elemental Composition ◽  
Field Emission ◽  
Cross Sections ◽  
Morphological Changes ◽  
Energy Dispersive ◽  
X Ray ◽  
Edx Analysis ◽  
Scanning Electron

Abstract:An innovative approach, combining field-emission scanning electron microscopy (FESEM) with energy dispersive X-ray spectroscopy (EDX) analysis, is presented to investigate the degradation mechanisms affecting tannin-dyed wool. In fact, tannin-dyed textiles are more sensitive to degradation then those dyed with other dyestuffs, even in the same conservation conditions.FESEM-EDX was first used to study a set of 48 wool specimens (artificially aged) dyed with several raw materials and mordants, and prepared according to historical dyeing recipes. EDX analysis was performed on the surface of wool threads and on their cross-sections. In addition, in order to validate the model formulated by the analysis of reference materials, several samples collected from historical and archaeological textiles were subjected to FESEM-EDX analysis.FESEM-EDX investigations enabled us to reveal the correlation between elemental composition and morphological changes. In addition, aging processes were clarified by studying changes in the elemental composition of wool from the protective cuticle to the fiber core in cross-sections. Morphological and elemental analysis of wool specimens and of archaeological and historical textiles showed that the presence of tannins increases wool damage, primarily by causing a sulfur decrease and fiber oxidation.

Light and Scanning Electron Microscopy of Human Spinal Ligaments

1985 ◽  
Vol 55 ◽  
Author(s):  
L.-H. Yahia ◽  
G. Drouin ◽  
C.-H. Rivard
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Connective Tissue ◽  
Idiopathic Scoliosis ◽  
Blood Vessels ◽  
Structural Changes ◽  
Loose Connective Tissue ◽  
Morphological Studies ◽  
Spinal Ligaments ◽  
Scanning Electron

ABSTRACTSpinal ligaments were obtained from normal and scoliotic individuals. Detailed morphological studies were carried out on the yellow, interspinous and supraspinous ligaments by light and scanning electron microscopy. Normal yellow ligaments are mostly constituted of dense elastin fibers with only a few collagen fibers and sparse blood vessels, while in normal interspinous and supraspinous ligaments, the presence of collagen is highly dominant. In the latter structures, the collagen fascicles are characterized by a regular waviness morphology. The fibrils constituting the fascicles appear either parallel or helical with respect to the fascicle axis. Structural changes are observed in the spinal ligaments of patients with congenital as well as idiopathic scoliosis. For yellow ligaments, only slight differences are found between normal and scoliotic specimens. However, alterations in collagen waviness and architecture are observed mainly in the supraspinous ligaments and to a lesser extent in the interspinous ligaments. In addition, increases in the cellularity, loose connective tissue and vessels are found in both forgoing ligaments. These results indicate that the more pronounced scoliosis-related changes occur in ligaments having the farthest distance from the axis of flexion-rotation.

scholarly journals Characterization of monoclonal IgG cryoglobulins: fine-structural and morphological analysis

Blood ◽  
1987 ◽  
Vol 69 (2) ◽  
pp. 677-681 ◽  
Author(s):  
DN Podell ◽  
CH Packman ◽  
J Maniloff ◽  
GN Abraham
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Morphological Analysis ◽  
Vascular Occlusion ◽  
Molecular Clusters ◽  
Morphological Properties ◽  
Transmission Electron ◽  
Thin Walled ◽  
Scanning Electron

Abstract The morphology of the amorphous, gelatinous, and crystalline varieties of monoclonal IgG cryoglobulins was analyzed by light and transmission and scanning electron microscopy. Each cryoglobulin had a characteristic fine structure that correlated with its gross morphology. Transmission electron microscopy showed that the amorphous precipitates were random and disorganized molecular clumps. In contrast, cryogels were thin-walled, well-organized, and hydrated strawlike clusters, whereas cryocrystals formed tightly compacted, highly structured molecular clusters. Crystals that formed in blood produced rouleaux, and analysis by scanning electron microscopy indicated that the crystals could form thick-walled, branching, macromolecular nets that could physically trap cells. The morphological properties provided visual impressions by which cryoglobulins could cause clinical disease secondary to vascular occlusion produced by self- associated IgG cryoglobulin molecules.

scholarly journals Rapid induction of morphological changes in human carcinoma cells A-431 by epidermal growth factors.

1979 ◽  
Vol 83 (1) ◽  
pp. 260-265 ◽  
Author(s):  
M Chinkers ◽  
J A McKanna ◽  
S Cohen
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Growth Factors ◽  
Morphological Changes ◽  
Carcinoma Cells ◽  
Human Carcinoma ◽  
Rapid Induction ◽  
Epidermal Growth ◽  
Scanning Electron

The morphological effects of epidermal growth factor (EGF) on human carcinoma cells A-431 have been examined by scanning electron microscopy. These flat polygonal cells normally exhibit only small membrane folds, but show extensive ruffling and extension of filopodia within 5 min of exposure to EGF at 37 degrees C. This ruffling activity is transient, subsiding within another 5--15 min, but several other changes in surface morphology follow. Within the first hour of exposure to the hormone, the cell surface becomes exceedingly smooth and the nuclei seem to protrude above the plane of the otherwise thin monolayer, giving the cells a "fried egg" appearance. Cells at the edges of colonies gradually retract from the substrate, leading to reorganization, by 12 h, of the monolayer into multilayered colonies. EGF thus induces both rapid and long-term alterations in the morphology of these epidermoid cells.

A comparison of scolex morphology between the plerocercoid and the adult of Calliobothrium verticillatum (Tetraphyllidea: Onchobothriidae)

1991 ◽  
Vol 69 (6) ◽  
pp. 1484-1488 ◽  
Author(s):  
J. N. Caira ◽  
T. R. Ruhnke
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Hermit Crab ◽  
Morphological Changes ◽  
Complex Surface ◽  
Apical Region ◽  
Apical Sucker ◽  
Mustelus Canis ◽  
Scanning Electron ◽  
Surface Changes

The morphological changes associated with the ontogenetic transformation of the scolex from larva to adult were investigated in the tapeworm Calliobothrium verticillatum (Rudolphi, 1819) van Beneden, 1850 by comparing the morphology of plerocercoids collected from the hermit crab Pagurus pollicaris Say, 1817 with that of adult worms collected from the shark Mustelus canis (Mitchell, 1815), using scanning electron microscopy. The apex of the scolex in young plerocercoids bore a conspicuous sucker. Each bothridium was flat and divided into four regions: an anterior oval region with two marginal bumps, and three posterior loculi clearly marked by two costae. The microthrix pattern was not determined for very young plerocercoids. The most conspicuous feature of older plerocercoids was the sucker on the apex of the scolex. The bothridia were concave, and each bore an apical region subdivided into three shallow suckers and three conspicuous loculi. Densely packed blade-like microtriches intermingled with filiform microtriches were visible on all surfaces of the scolex except the surface directly adjacent to the opening of the apical sucker of the scolex, which was entirely devoid of microtriches, and the proximal surfaces of the bothridia, which had only blade-like microtriches. The apical sucker was absent from the scolex adult specimens. The only remnant of this structure was a small knob on the apex of the scolex. The bothridia were concave and bore, from anterior to posterior, three conspicuous apical suckers arranged in a horizontal row adjacent to one another, two pairs of single-pronged hooks, and three conspicuous loculi. Densely packed blade-like microtriches were present on all proximal surfaces of the scolex. Microtriches were not present on the distal surfaces of the apical suckers of the bothridia, on the hooks, or on the distal surfaces of the loculi of the bothridia. The latter regions were covered with very small, round structures. The differences in the microthrix components of the various surfaces of the scolex between the plerocercoid and the adult suggest that complex surface changes occur during this phase of ontogeny. Comparison of the development of this species with that of other onchobothriids suggests that, in general, the number of loculi to be expected in the plerocercoid of an onchobothriid is one plus the number of posthook loculi in the adult.

Lower extremity peripheral vascular disease. Part 1: Scanning electron microscopy

1989 ◽  
Vol 79 (11) ◽  
pp. 527-535
Author(s):  
DJ McCarthy ◽  
N Abell ◽  
T Reed
Keyword(s):  
Electron Microscopy ◽  
At Risk ◽  
Scanning Electron Microscopy ◽  
Lower Extremity ◽  
Vascular Disease ◽  
Blood Vessels ◽  
Peripheral Vascular Disease ◽  
Peripheral Vascular ◽  
Tissue Alterations ◽  
Scanning Electron

The surgical loss of the foot or leg is vigorously resisted by podiatrists who are committed to the conservation of limbs at risk because of peripheral vascular disease. Pathologic changes in blood vessels may, however, progress to a point where no other option is available to the patient. This study assesses amputation of the extremities and investigates tissue alterations that can be identified in diseased blood vessels implicated in these circumstances. Gross pathology and scanning electron microscopy are examined in this, the first of a two-part study.

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