Improving TEM Fixation with Additives
Attempts to fix the gregarine Pterospora floridiensis (a protozoan parasite found in marine bristle worms) for TEM using a conventional glutaraldehyde and osmium protocol produced poor preservation of the outer pellicular membranes and internal membranous structures. However, fixation success was achieved using the well-known fixative additives tannic acid and potassium ferrocyanide, which improved membrane preservation, cytoplasmic integrity, and contrast in both the unencysted feeding stage of P. floridiensis as well as the difficult-to-fix cyst stage. The longer fixation times (24 hours) needed for the cysts were compatible with the modified fixatives. Interestingly, both additives were needed to improve fixation.
1973 ◽
Vol 31
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pp. 698-699
Keyword(s):
Structural preservation and absolute contrast of catalase crystal sections prepared with tannic acid
1983 ◽
Vol 41
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pp. 448-449
Keyword(s):
X Rays
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1981 ◽
Vol 39
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pp. 622-623
Keyword(s):
1992 ◽
Vol 50
(1)
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pp. 880-881
1987 ◽
Vol 45
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pp. 784-785
Keyword(s):
1962 ◽
Vol 07
(02)
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pp. 230-238
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Keyword(s):
2007 ◽
Vol 26
(1)
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pp. 9-20
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