scholarly journals 3D structure of nearby groups of galaxies

2014 ◽  
Vol 11 (S308) ◽  
pp. 207-208
Author(s):  
L. Makarova ◽  
D. Makarov ◽  
A. Klypin ◽  
S. Gottlöber
Keyword(s):  
Density Profile ◽  
3D Structure ◽  
Three Dimensional ◽  
Morphological Transformation ◽  
Groups Of Galaxies ◽  
Star Forming ◽  
Galaxy Groups ◽  
Dimensional Distribution ◽  
Dwarf Elliptical Galaxies ◽  
Absolute Magnitudes

AbstractUsing high accuracy distance estimates, we study the three-dimensional distribution of galaxies in five galaxy groups at a distance less than 5 Mpc from the Milky Way. Due to proximity of these groups our sample of galaxies is nearly complete down to extremely small dwarf galaxies with absolute magnitudes MB = -12. We find that the average number-density profile of the groups shows a steep power-law decline dn/dV ∼ R-3 at distances R=(100–500) kpc consistent with predictions of the standard cosmological model. We also find that there is no indication of a truncation or a cutoff in the density at the expected virial radius: the density profile extends at least to 1.5 Mpc. Vast majority of galaxies within 1.5 Mpc radius around group centres are gas-rich star-forming galaxies. Early-type galaxies are found only in the central ∼ 300 kpc region. Lack of dwarf spheroidal and dwarf elliptical galaxies in the field and in the outskirts of large groups is a clear indication that these galaxies experienced morphological transformation when they came close to the central region of forming galaxy group.

scholarly journals Exploring the properties of low-frequency radio emission and magnetic fields in a sample of compact galaxy groups using the LOFAR Two-Metre Sky Survey (LoTSS)

2019 ◽  
Vol 622 ◽  
pp. A23 ◽  
Author(s):  
B. Nikiel-Wroczyński ◽  
A. Berger ◽  
N. Herrera Ruiz ◽  
D. J. Bomans ◽  
S. Blex ◽  
...  
Keyword(s):  
Magnetic Field ◽  
Radio Emission ◽  
Low Frequency ◽  
Compact Groups ◽  
Groups Of Galaxies ◽  
Star Forming ◽  
Galaxy Groups ◽  
Sky Survey ◽  
Radio Studies ◽  
The Magnetic Field

We use the LOFAR Two-metre Sky Survey (LoTSS) Data Release I to identify the groups of galaxies (and individual galaxies) from the Hickson compact groups (HCG) and magnitude-limited compact groups (MLCG) samples that emit at the frequency of 150 MHz, characterise their radio emission (extended or limited to the galaxies), and compare new results to earlier observations and theoretical predictions. The detection of 73 systems (and 7 more – probably) out of 120, of which as many as 17 show the presence of extended radio structures, confirms the previous hypothesis of the common character of the magnetic field inside galaxy groups and its detectability. In order to investigate the future potential of low-frequency radio studies of galaxy groups, we also present a more detailed insight into four radio-emitting systems, for which the strength of the magnetic field inside their intergalactic medium (IGM) is calculated. The estimated values are comparable to that found inside star-forming galaxies, suggesting a dynamical and evolutionary importance of the magnetic field in galaxy groups.

scholarly journals The intergalactic medium in the cosmic web

2014 ◽  
Vol 11 (S308) ◽  
pp. 364-367
Author(s):  
Nicolas Tejos
Keyword(s):  
Intergalactic Medium ◽  
Three Dimensional ◽  
Baryonic Matter ◽  
One Dimensional ◽  
Star Forming ◽  
Galaxy Surveys ◽  
Dimensional Distribution ◽  
Formation And Evolution ◽  
The Universe ◽  
Cross Matching

AbstractThe intergalactic medium (IGM) accounts for ≳ 90% of baryons at all epochs and yet its three dimensional distribution in the cosmic web remains mostly unknown. This is so because the only feasible way to observe the bulk of the IGM is through intervening absorption line systems in the spectra of bright background sources, which limits its characterization to being one-dimensional. Still, an averaged three dimensional picture can be obtained by combining and cross-matching multiple one-dimensional IGM information with three-dimensional galaxy surveys. Here, we present our recent and current efforts to map and characterize the IGM in the cosmic web using galaxies as tracers of the underlying mass distribution. In particular, we summarize our results on: (i) IGM around star-forming and non-star-forming galaxies; (ii) IGM within and around galaxy voids; and (iii) IGM in intercluster filaments. With these datasets, we can directly test the modern paradigm of structure formation and evolution of baryonic matter in the Universe.

Methods for three-dimensional reconstruction of cellular components within a thick section

1986 ◽  
Vol 44 ◽  
pp. 18-21
Author(s):  
J. Frank ◽  
B. F. McEwen ◽  
M. Radermacher ◽  
C. L. Rieder
Keyword(s):  
Tilt Angle ◽  
Tomographic Reconstruction ◽  
3D Structure ◽  
Three Dimensional ◽  
Thick Section ◽  
Three Dimensional Reconstruction ◽  
Axis Ratio ◽  
Dimensional Reconstruction ◽  
Cellular Components

The tomographic reconstruction from multiple projections of cellular components, within a thick section, offers a way of visualizing and quantifying their three-dimensional (3D) structure. However, asymmetric objects require as many views from the widest tilt range as possible; otherwise the reconstruction may be uninterpretable. Even if not for geometric obstructions, the increasing pathway of electrons, as the tilt angle is increased, poses the ultimate upper limitation to the projection range. With the maximum tilt angle being fixed, the only way to improve the faithfulness of the reconstruction is by changing the mode of the tilting from single-axis to conical; a point within the object projected with a tilt angle of 60° and a full 360° azimuthal range is then reconstructed as a slightly elliptic (axis ratio 1.2 : 1) sphere.

Cryomicroscopy of crotoxin complex crystals at 400 KV

1989 ◽  
Vol 47 ◽  
pp. 826-827
Author(s):  
Jaap Brink ◽  
Wah Chiu
Keyword(s):  
Signal To Noise Ratio ◽  
3D Structure ◽  
Three Dimensional ◽  
Carbon Films ◽  
Depth Of Field ◽  
Basic Subunit ◽  
Ewald Sphere ◽  
Diffraction Patterns ◽  
Complex Crystals ◽  
Acidic Subunit

The crotoxin complex is a potent neurotoxin composed of a basic subunit (Mr = 12,000) and an acidic subunit (M = 10,000). The basic subunit possesses phospholipase activity whereas the acidic subunit shows no enzymatic activity at all. The complex's toxocity is expressed both pre- and post-synaptically. The crotoxin complex forms thin crystals suitable for electron crystallography. The crystals diffract up to 0.16 nm in the microscope, whereas images show reflections out to 0.39 nm2. Ultimate goal in this study is to obtain a three-dimensional (3D-) structure map of the protein around 0.3 nm resolution. Use of 100 keV electrons in this is limited; the unit cell's height c of 25.6 nm causes problems associated with multiple scattering, radiation damage, limited depth of field and a more pronounced Ewald sphere curvature. In general, they lead to projections of the unit cell, which at the desired resolution, cannot be interpreted following the weak-phase approximation. Circumventing this problem is possible through the use of 400 keV electrons. Although the overall contrast is lowered due to a smaller scattering cross-section, the signal-to-noise ratio of especially higher order reflections will improve due to a smaller contribution of inelastic scattering. We report here our preliminary results demonstrating the feasability of the data collection procedure at 400 kV.Crystals of crotoxin complex were prepared on carbon-covered holey-carbon films, quench frozen in liquid ethane, inserted into a Gatan 626 holder, transferred into a JEOL 4000EX electron microscope equipped with a pair of anticontaminators operating at −184°C and examined under low-dose conditions. Selected area electron diffraction patterns (EDP's) and images of the crystals were recorded at 400 kV and −167°C with dose levels of 5 and 9.5 electrons/Å, respectively.

Three-dimensional distribution of ribulose-1, 5-bisphosphate carboxylase/oxygemase during the early development of proplastids in dark-grown Euglena cells transferred to an inorganic medium

1990 ◽  
Vol 48 (3) ◽  
pp. 906-907
Author(s):  
Tomoko Ehara ◽  
Shuji Sumida ◽  
Tetsuaki Osafune ◽  
Eiji Hase
Keyword(s):  
Cell Suspension ◽  
Euglena Gracilis ◽  
Carbon Materials ◽  
Three Dimensional ◽  
Peripheral Region ◽  
Plastid Development ◽  
Bisphosphate Carboxylase ◽  
Inorganic Medium ◽  
Ribulose 1 ◽  
Dimensional Distribution

As shown previously, Euglena cells grown in Hutner’s medium in the dark without agitation accumulate wax as well as paramylum, and contain proplastids showing no internal structure except for a single prothylakoid existing close to the envelope. When the cells are transferred to an inorganic medium containing ammonium salt and the cell suspension is aerated in the dark, the wax was oxidatively metabolized, providing carbon materials and energy 23 for some dark processes of plastid development. Under these conditions, pyrenoid-like structures (called “pro-pyrenoids”) are formed at the sites adjacent to the prolamel larbodies (PLB) localized in the peripheral region of the proplastid. The single prothylakoid becomes paired with a newly formed prothylakoid, and a part of the paired prothylakoids is extended, with foldings, in to the “propyrenoid”. In this study, we observed a concentration of RuBisCO in the “propyrenoid” of Euglena gracilis strain Z using immunoelectron microscopy.

Detection, Averaging, and 3D Reconstruction of Biological Specimens on Hypercubes (Transputer-based) Computers

1990 ◽  
Vol 48 (1) ◽  
pp. 454-455
Author(s):  
Jose-Maria Carazo ◽  
I. Benavides ◽  
S. Marco ◽  
J.L. Carrascosa ◽  
E.L. Zapata
Keyword(s):  
3D Reconstruction ◽  
3D Structure ◽  
Three Dimensional ◽  
Software Tools ◽  
Mapping Method ◽  
Computer Architectures ◽  
Parallel Computer ◽  
Reconstruction Process ◽  
Computing Power ◽  
Order Of Magnitude

Obtaining the three-dimensional (3D) structure of negatively stained biological specimens at a resolution of, typically, 2 - 4 nm is becoming a relatively common practice in an increasing number of laboratories. A combination of new conceptual approaches, new software tools, and faster computers have made this situation possible. However, all these 3D reconstruction processes are quite computer intensive, and the middle term future is full of suggestions entailing an even greater need of computing power. Up to now all published 3D reconstructions in this field have been performed on conventional (sequential) computers, but it is a fact that new parallel computer architectures represent the potential of order-of-magnitude increases in computing power and should, therefore, be considered for their possible application in the most computing intensive tasks.We have studied both shared-memory-based computer architectures, like the BBN Butterfly, and local-memory-based architectures, mainly hypercubes implemented on transputers, where we have used the algorithmic mapping method proposed by Zapata el at. In this work we have developed the basic software tools needed to obtain a 3D reconstruction from non-crystalline specimens (“single particles”) using the so-called Random Conical Tilt Series Method. We start from a pair of images presenting the same field, first tilted (by ≃55°) and then untilted. It is then assumed that we can supply the system with the image of the particle we are looking for (ideally, a 2D average from a previous study) and with a matrix describing the geometrical relationships between the tilted and untilted fields (this step is now accomplished by interactively marking a few pairs of corresponding features in the two fields). From here on the 3D reconstruction process may be run automatically.

scholarly journals A Novel Pseudomonas geniculata AGE Family Epimerase/Isomerase and Its Application in d-Mannose Synthesis

Foods ◽  
2020 ◽  
Vol 9 (12) ◽  
pp. 1809
Author(s):  
Zhanzhi Liu ◽  
Ying Li ◽  
Jing Wu ◽  
Sheng Chen
Keyword(s):  
3D Structure ◽  
Three Dimensional ◽  
Optimal Temperature ◽  
Reaction Conditions ◽  
Enzymatic Production ◽  
Physiological Properties ◽  
Potential Applications ◽  
Kinetics Of ◽  
Optimal Reaction ◽  
Gene Age

d-mannose has exhibited excellent physiological properties in the food, pharmaceutical, and feed industries. Therefore, emerging attention has been applied to enzymatic production of d-mannose due to its advantage over chemical synthesis. The gene age of N-acetyl-d-glucosamine 2-epimerase family epimerase/isomerase (AGEase) derived from Pseudomonas geniculata was amplified, and the recombinant P. geniculata AGEase was characterized. The optimal temperature and pH of P. geniculata AGEase were 60 °C and 7.5, respectively. The Km, kcat, and kcat/Km of P. geniculata AGEase for d-mannose were 49.2 ± 8.5 mM, 476.3 ± 4.0 s−1, and 9.7 ± 0.5 s−1·mM−1, respectively. The recombinant P. geniculata AGEase was classified into the YihS enzyme subfamily in the AGE enzyme family by analyzing its substrate specificity and active center of the three-dimensional (3D) structure. Further studies on the kinetics of different substrates showed that the P. geniculata AGEase belongs to the d-mannose isomerase of the YihS enzyme. The P. geniculata AGEase catalyzed the synthesis of d-mannose with d-fructose as a substrate, and the conversion rate was as high as 39.3% with the d-mannose yield of 78.6 g·L−1 under optimal reaction conditions of 200 g·L−1d-fructose and 2.5 U·mL−1P. geniculata AGEase. This novel P. geniculata AGEase has potential applications in the industrial production of d-mannose.

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