Expression of concern Genetically modified soya bean in rabbit feeding: detection of DNA fragments and evaluation of metabolic effects by enzymatic analysis – EXPRESSION OF CONCERN (UPDATE)

AbstractThe presence of DNA fragments in tissues from rabbits given genetically modified (GM) soya-bean meal (solvent extracted) was investigated by using the polymerase chain reaction (PCR) approach. Moreover, the possible effects on cell metabolism were evaluated by determination of several specific enzymes in serum, heart, skeletal muscle, liver and kidney. The chloroplast sequence for tRNA Leu by using the Clor1/Clor2 primers designed on chloroplast trnL sequence was clearly detected. On the contrary, two couples of species specific primers for conventional (Le1-5/Le 1-3 which amplifies the soya bean lectin gene) and genetically modified (35S1/35S2 which amplifies the 35S CMV promoter that is present in the genomic structure of GM soya bean) soya bean were not found in all samples. No differences in enzyme levels were detected in serum, but a significant increase of lactic dehydrogenase, mainly concerning the LDH1 isoenzyme was found in particular in kidney and heart but not in the muscle, thus suggesting a potential alteration in the local production of the enzyme. Finally, no significant differences were detected concerning body weight, fresh organ weights and no sexual differences were detected.

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Expression of concernGenetically modified soya bean in rabbit feeding: detection of DNA fragments and evaluation of metabolic effects by enzymatic analysis - EXPRESSION OF CONCERN

animal ◽

10.1017/s1751731117000027 ◽

2017 ◽

Vol 11 (5) ◽

pp. 910 ◽

Cited By ~ 1

Author(s):

R. Tudisco ◽

P. Lombardi ◽

F. Bovera ◽

D. dˇAngelo ◽

M.I. Cutrignelli ◽

...

Keyword(s):

Soya Bean ◽

Metabolic Effects ◽

Dna Fragments ◽

Enzymatic Analysis

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Highly reproducible capillary gel electrophoresis (CGE) of DNA fragments using uncoated columns. Detection of genetically modified maize by PCR-cGE

Journal of Separation Science ◽

10.1002/1615-9314(20020601)25:9<577::aid-jssc577>3.0.co;2-7 ◽

2002 ◽

Vol 25 (9) ◽

pp. 577-583 ◽

Cited By ~ 30

Author(s):

Virginia García-Cañas ◽

Ramón González ◽

Alejandro Cifuentes

Keyword(s):

Gel Electrophoresis ◽

Genetically Modified ◽

Dna Fragments ◽

Genetically Modified Maize ◽

Capillary Gel Electrophoresis

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Detection of corn intrinsic and recombinant DNA fragments and Cry1Ab protein in the gastrointestinal contents of pigs fed genetically modified corn Bt11 1

Journal of Animal Science ◽

10.2527/2003.81102546x ◽

2003 ◽

Vol 81 (10) ◽

pp. 2546-2551 ◽

Cited By ~ 75

Author(s):

E. H. Chowdhury ◽

H. Kuribara ◽

A. Hino ◽

P. Sultana ◽

O. Mikami ◽

...

Keyword(s):

Recombinant Dna ◽

Genetically Modified ◽

Dna Fragments ◽

Cry1ab Protein

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The presence or absence of transgenic and endogenous plant DNA fragments in the blood, tissues and digesta of broilers consuming genetically modified dietary ingredients

Proceedings of the British Society of Animal Science ◽

10.1017/s1752756200009984 ◽

2005 ◽

Vol 2005 ◽

pp. 87-87

Author(s):

E. R. Deaville ◽

B. C. Maddison

Keyword(s):

Genetically Modified ◽

Gm Crops ◽

Human Consumption ◽

Dna Fragments ◽

Safety Concerns ◽

Plant Dna ◽

Soyabean Meal ◽

Maize Grain ◽

Endogenous Plant

The global area sown to genetically modified (GM) crops has increased rapidly from 1.7 million ha in 1996 to 67.7 million ha in 2003 (James, 2003). While GM crops have been shown to be substantially equivalent (e.g. Clarke and Ipharraguerre, 2000), the use of GM crops (e.g. maize grain) or components/products from them (e.g. soyabean meal) in livestock diets has raised a number of safety concerns including, the potential for transgenic DNA to transfer to animal-derived products intended for human consumption. Therefore, the aim was to determine the presence or absence of transgenic and endogenous plant DNA fragments in the blood, tissues and digesta of broilers consuming GM-based diets.

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Fate of transgenic DNA and evaluation of metabolic effects in goats fed genetically modified soybean and in their offsprings

animal ◽

10.1017/s1751731110000728 ◽

2010 ◽

Vol 4 (10) ◽

pp. 1662-1671 ◽

Cited By ~ 28

Author(s):

R. Tudisco ◽

V. Mastellone ◽

M.I. Cutrignelli ◽

P. Lombardi ◽

F. Bovera ◽

...

Keyword(s):

Genetically Modified ◽

Metabolic Effects ◽

Genetically Modified Soybean

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Monitoring and traceability of genetically modified soya bean event GTS 40-3-2 during soya bean protein concentrate and isolate preparation

Royal Society Open Science ◽

10.1098/rsos.201147 ◽

2020 ◽

Vol 7 (10) ◽

pp. 201147

Author(s):

Yan Du ◽

Fusheng Chen ◽

Chen Chen ◽

Kunlun Liu

Keyword(s):

Raw Materials ◽

Genetically Modified ◽

Soya Bean ◽

Practical Significance ◽

Protein Concentrate ◽

Consumer Rights ◽

By Products ◽

Actual Mass ◽

Cp4 Epsps ◽

Bean Protein

To evaluate DNA fragmentation and GMO quantification during soya bean protein concentrate and isolate preparation, genetically modified soya bean event GTS 40-3-2 (Roundup Ready TM soya bean, RRS) was blended with conventional soya beans at mass percentages of 0.9%, 2%, 3%, 5% and 10%. Qualitative PCR and real-time PCR were used to monitor the taxon-specific lectin and exogenous cp4 epsps target levels in all of the main products and by-products, which has practical significance for RRS labelling threshold and traceability. Along the preparation chain, the majority of DNA was distributed in main products, and the DNA degradation was noticed. From a holistic perspective, the lectin target degraded more than cp4 epsps target during both of the two soya bean proteins preparations. Therefore, the transgenic contents in the final protein products were higher than the actual mass percentages of RRS in raw materials. Our results are beneficial to the improvement of GMO labelling legislation and the protection of consumer rights.

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