Tannin bioassay using semi-automated gas production technique

2005 ◽  
Vol 2005 ◽  
pp. 218-218
Author(s):  
E.F. Nozella ◽  
S.L.S. Cabral Filho ◽  
I.C.S. Bueno ◽  
P.B. Godoy ◽  
C. Longo ◽  
...  
Keyword(s):  
Polyethylene Glycol ◽  
Arid Regions ◽  
Animal Feed ◽  
Gas Production ◽  
Production Technique ◽  
Nutritional Factors ◽  
High Affinity ◽  
Nutritional Compounds ◽  
Phenolics Compounds

Brazil has arid regions where livestock production is limited by forage source. However, some native herbaceous browses have a dry tolerance and had been used as animal feed. Some of those plants have anti nutritional compounds such as tannins that can interfere on intake and digestibility. Tannins have a high affinity to proteins and could make these molecules unavailable for animal. Compounds as polyethylene glycol (PEG) has been used on tannin studies, because it has more affinity with tannins than proteins. Based on that, it is possible to evaluate the nutritive potential of tanniniferous plants, using PEG in gas based techniques for assessing anti nutritional factors in tanniniferous plants for ruminants. The aim of this work was to investigate the effects of different treatments (oven-, shade- and sun-drying and treatment with urea) on phenolics compounds and on the biological activity of tannins using the in vitro gas method with the addition of polyethylene glycol (PEG).

Tannin bioassay using semi-automated and manual gas production techniques for Brazilian browses

2003 ◽  
Vol 2003 ◽  
pp. 191-191
Author(s):  
S. L. S. Cabral Filho ◽  
I. C. S. Bueno ◽  
E. F. Nozella ◽  
A. L. Abdalla ◽  
D. M. S. S. Vitti
Keyword(s):  
Polyethylene Glycol ◽  
Animal Feed ◽  
Rumen Fermentation ◽  
Gas Production ◽  
Nutritional Factors ◽  
Production Techniques ◽  
Bioassay Technique ◽  
Nutritional Compounds

Native herbaceous browses at Northeast of Brazil have a dry tolerance and have been used as animal feed. Some of those plants have anti nutritional compounds such as tannins that can interfere on their intake and digestibility. Polyethylene glycol (PEG) has been used in gas-based techniques for assessing anti nutritional factors in tanniniferous plants for ruminants. The objective of this work was to compare the tannin bioassay technique using the semi-automated (Reading Pressure Technique - RPT) and manual (syringe) gas production techniques to evaluate the tannin effect upon in vitro rumen fermentation, using PEG as an inhibitor of tannin effects.

Tannin contents and in vitro digestibility of Brazilian browses

2002 ◽  
Vol 2002 ◽  
pp. 133-133
Author(s):  
Eduardo F. Nozella ◽  
Sergio L. S. Cabral Filho ◽  
Ives C. S. Bueno ◽  
Adibe L. Abdalla ◽  
Dorinha M. S. S. Vitti
Keyword(s):  
Arid Regions ◽  
Animal Feed ◽  
Livestock Production ◽  
Rumen Fermentation ◽  
In Vitro Digestibility ◽  
Herbaceous Legumes ◽  
High Affinity ◽  
Nutritional Compounds

Brazil has arid regions where livestock production is limited by forage source. However, some native herbaceous legumes have a dry tolerance and had been used as animal feed. Some of those plants have anti nutritional compounds such as tannins that and can interfere on intake and digestibility of these plants. Tannins have a high affinity with proteins and could make these molecules unavailable for animal. Compounds as polyvinylpolypyrrolidone (PVPP) have been used on tannin studies, because it has more affinity with tannins than proteins. Based on that, it is possible to evaluate the nutritive potential of tanniniferous plants, using PVPP as an inhibitor of tannin effects. The aim of this work was to evaluate the effect of tannins on in vitro rumen fermentation.

Bioassay for measuring tannin effects based on gas production technique. 1. Binding compounds

2005 ◽  
Vol 2005 ◽  
pp. 215-215
Author(s):  
I.C.S. Bueno ◽  
P.B. Godoy ◽  
S.L.S. Cabral Filho ◽  
R.S. Dias ◽  
C. Longo ◽  
...  
Keyword(s):  
Chemical Analysis ◽  
Biological Effects ◽  
Biological Response ◽  
Gas Production ◽  
Production Technique ◽  
Nutritional Factors ◽  
Lack Of Information ◽  
Tropical Legumes ◽  
The Tropics

Anti nutritional factors in tropical legumes are very common and the chemical analysis for tannins has become an important tool to evaluate alternative ruminant feeds in the tropics. However, frequently results from tannin chemical analysis are not in agreement with biological response when animals are fed those feeds. There is a lack of information concerning the biological effects (activity or reactivity) of tannins on ruminants. Usually the effects of tannins are tested in vitro by adding compounds with capacity of biding tannins. The aim of this work was to compare two binding compounds to evaluate the effect of tannins of tanniniferous feeds for ruminants.

Evaluation of artichoke (Cynara scolymus) by-product using the semi-automatic In vitro gas production technique

2007 ◽  
Vol 2007 ◽  
pp. 208-208 ◽  
Author(s):  
Sobhy Mohamed Abdallah Sallam ◽  
Ives Claudio da Silva Bueno ◽  
Patricia Barboza Godoy ◽  
Eduardo Fernando Nozella ◽  
Adibe Luiz Abdalla ◽  
...  
Keyword(s):  
Animal Feed ◽  
Gas Production ◽  
Production Technique ◽  
Cynara Scolymus ◽  
Major Constraint ◽  
In Vitro Gas Production ◽  
Wide Range ◽  
Animal Feeding ◽  
By Products

The major constraint limiting the development of livestock production in many developing countries is inadequacy of animal feed; although there are a wide range of valuable by-products and residues eligible to complement it. To estimate whether a material is appropriate for animal feeding, in vitro gas production (GP) technique have been used to evaluate the potential of different classes of feedstuffs to supply nutrients to ruminants. Thus, the fermentation characteristics of artichoke by-product (Cynara scolymus), alfalfa and Tifton 85 (Cynodon sp) hay were evaluated by in vitro GP technique.

Estimation of fermentable energy by the automatic in vitro gas production technique for a selection of feedstuffs incubated alone and in combination

1998 ◽  
Vol 1998 ◽  
pp. 69-69
Author(s):  
S. Fakhri ◽  
A. R. Moss ◽  
D.I. Givens ◽  
E. Owen
Keyword(s):  
Organic Matter ◽  
Incubation Period ◽  
Gas Production ◽  
Production Technique ◽  
In Vitro Gas Production ◽  
Fe Content ◽  
The Individual ◽  
Gas Volume ◽  
Selection Of

The gas production (GP) technique has previously been used to estimate the gas volume (fermentable energy (FE)) of compound feed ingredients for ruminants (Newbold et al., 1996). It was shown that the FE content of feed mixtures was represented by the combination of the total gas from the incubation of the individual feeds. However this additivity might not be consistent throughout the incubation period. The objectives were to test whether 1. other GP parameters give better estimates of FE for simple mixtures and are they additive; 2. whether organic matter apparently degraded in the rumen (OMADR) explain differences in GP; and 3. to find out if there are any other better measures than OMADR for estimating FE.

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