Tannin contents and in vitro digestibility of Brazilian browses

2002 ◽  
Vol 2002 ◽  
pp. 133-133
Author(s):  
Eduardo F. Nozella ◽  
Sergio L. S. Cabral Filho ◽  
Ives C. S. Bueno ◽  
Adibe L. Abdalla ◽  
Dorinha M. S. S. Vitti
Keyword(s):  
Arid Regions ◽  
Animal Feed ◽  
Livestock Production ◽  
Rumen Fermentation ◽  
In Vitro Digestibility ◽  
Herbaceous Legumes ◽  
High Affinity ◽  
Nutritional Compounds

Brazil has arid regions where livestock production is limited by forage source. However, some native herbaceous legumes have a dry tolerance and had been used as animal feed. Some of those plants have anti nutritional compounds such as tannins that and can interfere on intake and digestibility of these plants. Tannins have a high affinity with proteins and could make these molecules unavailable for animal. Compounds as polyvinylpolypyrrolidone (PVPP) have been used on tannin studies, because it has more affinity with tannins than proteins. Based on that, it is possible to evaluate the nutritive potential of tanniniferous plants, using PVPP as an inhibitor of tannin effects. The aim of this work was to evaluate the effect of tannins on in vitro rumen fermentation.

Tannin bioassay using semi-automated gas production technique

2005 ◽  
Vol 2005 ◽  
pp. 218-218
Author(s):  
E.F. Nozella ◽  
S.L.S. Cabral Filho ◽  
I.C.S. Bueno ◽  
P.B. Godoy ◽  
C. Longo ◽  
...  
Keyword(s):  
Polyethylene Glycol ◽  
Arid Regions ◽  
Animal Feed ◽  
Gas Production ◽  
Production Technique ◽  
Nutritional Factors ◽  
High Affinity ◽  
Nutritional Compounds ◽  
Phenolics Compounds

Brazil has arid regions where livestock production is limited by forage source. However, some native herbaceous browses have a dry tolerance and had been used as animal feed. Some of those plants have anti nutritional compounds such as tannins that can interfere on intake and digestibility. Tannins have a high affinity to proteins and could make these molecules unavailable for animal. Compounds as polyethylene glycol (PEG) has been used on tannin studies, because it has more affinity with tannins than proteins. Based on that, it is possible to evaluate the nutritive potential of tanniniferous plants, using PEG in gas based techniques for assessing anti nutritional factors in tanniniferous plants for ruminants. The aim of this work was to investigate the effects of different treatments (oven-, shade- and sun-drying and treatment with urea) on phenolics compounds and on the biological activity of tannins using the in vitro gas method with the addition of polyethylene glycol (PEG).

Tannin bioassay using semi-automated and manual gas production techniques for Brazilian browses

2003 ◽  
Vol 2003 ◽  
pp. 191-191
Author(s):  
S. L. S. Cabral Filho ◽  
I. C. S. Bueno ◽  
E. F. Nozella ◽  
A. L. Abdalla ◽  
D. M. S. S. Vitti
Keyword(s):  
Polyethylene Glycol ◽  
Animal Feed ◽  
Rumen Fermentation ◽  
Gas Production ◽  
Nutritional Factors ◽  
Production Techniques ◽  
Bioassay Technique ◽  
Nutritional Compounds

Native herbaceous browses at Northeast of Brazil have a dry tolerance and have been used as animal feed. Some of those plants have anti nutritional compounds such as tannins that can interfere on their intake and digestibility. Polyethylene glycol (PEG) has been used in gas-based techniques for assessing anti nutritional factors in tanniniferous plants for ruminants. The objective of this work was to compare the tannin bioassay technique using the semi-automated (Reading Pressure Technique - RPT) and manual (syringe) gas production techniques to evaluate the tannin effect upon in vitro rumen fermentation, using PEG as an inhibitor of tannin effects.

scholarly journals Evaluation of Agro-Industrial by Products as Potential Local Feed for Ruminant Animals: Chemical Composition, Fiber Fractions and In Vitro Rumen Fermentation

2019 ◽  
Vol 20 (3) ◽  
pp. 155
Author(s):  
Said Mirza Pratama ◽  
Sitti Wajizah ◽  
Anuraga Jayanegara ◽  
Samadi Samadi
Keyword(s):  
Chemical Composition ◽  
Animal Feed ◽  
Rumen Fermentation ◽  
Coffee Pulp ◽  
Fiber Fractions ◽  
Ruminant Animals ◽  
Coconut Meal ◽  
By Products ◽  
In Vitro Rumen Fermentation

Animals are considered as source of protein should be improved their productivity with the minimum cost production. Agro-industrial by products have been used as animal feed to reduce feed cost. The purpose of this study is to evaluate agro-industrial by product in Aceh as potential local feed for ruminant animals based on chemical composition, fiber fraction and in vitro rumen fermentation. There were eight sources of agro-industrial by products (sago residues, coconut meal, soybean-ketchup by product, coffee pulp, cacao pod, sago tree, corncob, and rice brand) which were collected from 3 different locations in Aceh.  All agro-industrial by product samples were dried at 600C for 24 h and ground to pass a 1 mm sieve. Grounded samples were analyzed to determine chemical composition, fiber fractions and in vitro rumen fermentation. Incubation was conducted at temperature 390C for 48 h in water bath with three replicates. Data for in vitro rumen fermentation were statically calculated by using SPSS differences between treatments were stated (P≤0.05) by using Duncan Multiple Range Test (DMRT). The results indicated that agro industrial by product from coconut meal, ketchup residues, coffee by product, cacao by product, and rice brand can be used as source of protein and industrial by product from sago by product, sago tree and corncob can be used as source of energy. Neutral detergent  insoluble  CP (NDICP) and Acid detergent insoluble CP (ADICP)  were relatively high for sago by product, sago tree and rice brand but relatively low for cacao by product and corncob. The value of incubated pH for most feed samples was in the normal range. In vitro dry matter digestibility (IVDMD) and in vitro organic matter  digestibility (IVOMD) were significantly difference (P≤0.05) each agro-industrial by products with the highest for sago and the lowest for coffee by product. In conclusion, agro-industrial by products had a potential feed for ruminant animals both as protein and energy sources. However, feed ingredients with high fiber content and low degradability, further treatments such as physical, chemical and biological treatments were required to improve the feed quality. 

scholarly journals Protein enrichment of cactus pear (Opuntia ficus - indica Mill) using Saccharomyces cerevisiae in solid-state fermentation

2005 ◽  
Vol 48 (spe) ◽  
pp. 161-168 ◽  
Author(s):  
Lúcia de Fátima Araújo ◽  
Ariosvaldo Nunes Medeiros ◽  
Américo Perazzo Neto ◽  
Líbia de Sousa Conrado Oliveira ◽  
Flávio Luiz Honorato da Silva
Keyword(s):  
Dry Matter ◽  
Solid Medium ◽  
Animal Feed ◽  
In Vitro Digestibility ◽  
Cactus Pear ◽  
Protein Supplements ◽  
Dry Matter Digestibility ◽  
Matter Production ◽  
Gross Energy

The microbial protein bioconversion of cactus pear by yeast in solid medium was studied. Three cultivation variables used were: inoculum's concentrations (5, 10 and 15 %), substrate layer thickness (2, 4 and 6 cm) and temperature (30, 34 and 38 ºC). The rate of dry matter production and total protein were determined. Results obtained were variance analysis, gross energy and in vitro dry matter digestibility. The maximum protein amount achieved for the conditions studied in the present work was higher than 26 %, which was compatible or greater than those of conventional concentrates of protein supplements used for animal feed. The protein concentrate of cactus pear had a higher in vitro digestibility index (95.8 %) and did not show any changes in the gross energy value when compared to that of the cactus pear in natura.

scholarly journals Development of a Novel Enzymatic Pretreatment for Improving the Digestibility of Protein in Feather Meal

2019 ◽  
Vol 1 (4) ◽  
pp. 475-484 ◽  
Author(s):  
Pfeuti ◽  
Osborne ◽  
Shoveller ◽  
Ignatz ◽  
Bureau
Keyword(s):  
Nutritional Value ◽  
Animal Feed ◽  
In Vitro Digestibility ◽  
Optimal Conditions ◽  
Feather Meal ◽  
Alkaline Serine Protease ◽  
Enzymatic Pretreatment ◽  
Full Factorial ◽  
Degradable Protein

This study describes the process of developing an enzymatic pretreatment to improvethe nutritional value of feather meal (FeM). In a first experiment, a full factorial design was used toexamine the effects of various incubation conditions on the solubilization of nitrogen in FeM. Weincubated FeM for 3 h with various levels of a commercial alkaline serine protease (Savinase®16L), sodium sulphite (Na2SO3), and digestion buffer. A Savinase® 16L level of 3% (%FeM v/w),Na2SO3 level of 3% (%FeM w/w), and digestion buffer level of 500% (%FeM w/w) were identifiedas the optimal conditions. Under these optimal conditions, 45% of the nitrogen in FeM wassolubilized. In a second experiment, we evaluated the effect of more economically sustainableincubation conditions on the in vitro digestibility of protein (pepsin-HCl digestibility andmultistep protein evaluation) in FeM. Two FeMs were incubated with 0.5% Savinase® 16L (%FeMv/w), 2% Na2SO3 (%FeM w/w), and 200% buffer (%FeM w/w) for 24 h. The pretreatment improvedpepsin-HCl digestibility by 7%–16% and the total tract degradable protein content by 14%–50%.Accordingly, this novel pretreatment could be applied in the animal feed industry to improve thenutritional value of FeM.

Effects of the level of concentrate in the diet of the donor animal on the in vitro digestibility of hay

1998 ◽  
Vol 1998 ◽  
pp. 177-177
Author(s):  
O. Bochi-Brum ◽  
M.D. Carro ◽  
C. Valdés ◽  
J.S. González ◽  
S. López
Keyword(s):  
Microbial Activity ◽  
Rumen Fermentation ◽  
In Vitro Digestibility ◽  
Donor Animal ◽  
Uncontrolled Variation

The inoculum represents one of the greatest sources of uncontrolled variation in digestibility estimates via in vitro rumen fermentation methods (Marten and Barnes 1980). Microbial activity of rumen inoculum may be influenced by animal and diet factors. The objective of this work was to evaluate the effects of the level of inclusion of concentrate in the diet of the donor animal on the measurement of the in vitro digestibility of hay.

Comparison of rumen fluid from South African game species and from sheep to digest tanniniferous browse

2001 ◽  
Vol 52 (4) ◽  
pp. 453 ◽  
Author(s):  
R. J. Jones ◽  
J. H. F. Meyer ◽  
F. M. Bechaz ◽  
M. A. Stoltz ◽  
B. Palmer ◽  
...  
Keyword(s):  
Adverse Effects ◽  
South African ◽  
Dry Matter ◽  
Animal Species ◽  
Animal Feed ◽  
Rumen Fluid ◽  
Microbial Populations ◽  
In Vitro Digestibility ◽  
Rumen Bacteria

The hypothesis that South African game animals that use browse containing condensed tannins (CT) could have rumen microbial populations better able to ferment tanniniferous forages was explored. Rumen fluid (RF) from a range of browsing ruminants was used to ferment tanniniferous forages in vitro and the results were compared with the values obtained with rumen fluid from sheep. Leaves of the shrub legumes Leucaena leucocephala, L. pallida, L. trichandra, Calliandra calothyrsus,Gliricidia sepium, and Acacia boliviana were used as substrates. In vitro digestibility of dry matter (IVDMD) and nitrogen (IVND) was measured in the presence or absence of polyethylene glycol (PEG) 4000 to assess the adverse effects of the CT in the feeds. Rumen fluid from giraffe, greater kudu, eland, duiker, impala, nyala, goat, and gnu was compared with that from sheep. Ability to metabolise or block the adverse effects of CT was expected to result in only a small or no response to PEG in terms of IVDMD and IVND with rumen fluid from browsers, and therefore to result in a significant animal feed and animal PEG interaction. There were no significant (P > 0.05) animal feed interactions. For every animal species there was a response to PEG in terms of IVND, and this response was linearly related to the CT in the feed as measured by PEG binding using 14C-labelled PEG. Rumen fluid from browsers had a lower pH (5.78 0.060 v. 6.68 0.052) and a higher dry matter content (1473 102 mg v. 415 4.3 mg/100 mL RF) than that of grazers (sheep and gnu). However, when measured at 2 pH levels (6.7 and 5.8), IVDMD and IVND of the feeds were not improved at the lower pH with rumen fluid from the browsers. Rather, digestibility was depressed at the lower pH with rumen fluid from all animal species. We conclude from these in vitro studies that rumen microbial populations from browsers are not able to ferment tanniniferous forages better than rumen microbial populations from grazers. It seems likely that these browsers do not have rumen bacteria capable of degrading the CT in these shrub legumes. Production on such tanniniferous feeds may well depend on tannin binding with proline-rich saliva rather than on metabolism of or tolerance to CT by rumen bacteria.

scholarly journals ADDITION OF TOFU WASTE TO CONCENTRAT FEED ON RUMINANT ANIMAL VALUE

2019 ◽  
Vol 1 (2) ◽  
Author(s):  
Engkus Ainul Yakin ◽  
Sri Sukaryani ◽  
Catur Suci Purwati ◽  
Desi Lestari
Keyword(s):  
Crude Protein ◽  
Dry Matter ◽  
Nutritional Value ◽  
Animal Feed ◽  
Crude Fiber ◽  
In Vitro Digestibility ◽  
Dry Matter Content ◽  
Ruminant Animal ◽  
Significant Difference

The purpose of this study was to determine the effect of the addition of tofu waste in concentrate feed on the nutritional value of ruminant animal feed. Concentrate feed used in this study uses concentrated feed for sheep. Tofu waste was obtained from the place of making tofu in Girimarto sub-district, Wonogiri Regency. The research method used was concentrate feed for sheep given the addition of tofu waste. The study used 3 treatments namely T0 = Concentrate without the addition of tofu waste (control), T1 = Concentrate with 10% tofu waste addition, and T2 = Concentrate with 20% tofu waste addition. The results of the study were analyzed in the laboratory to determine the nutritional value of sheep feed. The study was designed with a completely randomized design. Observation parameters of nutritional value of feed include dry matter, crude protein, crude fiber and in vitro digestibility. The results showed that dry matter content was 78.54 ± 0.23% and crude protein was 16.63 ± 0.46% with the addition of tofu waste until 20% showed a significant difference, whereas crude fiber was 20.37 ± 0.48 % showed no significant difference, while the dry matter digestibility and organic matter digestibility  showed no significant difference. The conclusion of this study was the addition of tofu waste in the concentrate had an effect on the dry matter and crude protein, but not significantly different on crude fiber. The addition of tofu waste to the concentrate did not affect the digestibility of dry matter or the digestibility of organic matter.Key words : Animal feed, concentrate,  in vitro digestibility, tofu waste

scholarly journals Nutritional valorisation of cane (Arundo donax) by treatment with sodium hydroxide

2021 ◽  
Vol 51 (4) ◽  
pp. 437-443
Author(s):  
S.M.P. Teixeira ◽  
C.S.A.M. Maduro Dias ◽  
C.F.M. Vouzela ◽  
J.S. Madruga ◽  
A.E.S. Borba
Keyword(s):  
Sodium Hydroxide ◽  
Dry Matter ◽  
Nutritional Value ◽  
Animal Feed ◽  
Arundo Donax ◽  
In Vitro Digestibility ◽  
Acid Detergent Fibre ◽  
Naoh Solution ◽  
Air Circulation

This study evaluated the effects of sodium hydroxide (NaOH) treatment on the nutritional value of Arundo donax (A. donax). Its ultimate goal was to develop an environmentally friendly animal feed alternative that could be produced sustainably while combatting the spread of A. donax. Plants were collected and dried at 60 °C in an oven with controlled air circulation to determine the dry matter (DM) content. The dry plant material was then sprinkled with an NaOH solution of 2%, 4%, 6%, or 8% and placed in leak-proof containers for four weeks. The chemical and in vitro digestibility properties of treated and untreated A. donax samples were analysed in triplicate. The treatment led to significant decreases in neutral detergent fibre (NDF) from 81.06% DM to 69.39% DM, acid detergent fibre (ADF) from 46.99% DM to 43.19% DM, and ether extract (EE) from 2.06% DM to 1.39% DM, in the untreated samples compared with those treated with 8% NaOH, respectively. In addition, DM digestibility increased from 24.61% to 33.78%, ash content from 11.75% DM to 19.92% DM, and ADL from 7.43% M to 15.38% DM. Thus, treatment of A. donax with an 8% solution of NaOH concentration improved its nutritional value.

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