On-Line Combination of High Performance Liquid Chromatography with Comprehensive Two-Dimensional Gas Chromatography-Triple Quadrupole Mass Spectrometry: A Proof of Principle Study

2015 ◽  
Vol 87 (3) ◽  
pp. 1911-1918 ◽  
Author(s):  
Mariosimone Zoccali ◽  
Peter Quinto Tranchida ◽  
Luigi Mondello
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Triple Quadrupole ◽  
Two Dimensional ◽  
Quadrupole Mass ◽  
On Line ◽  
Proof Of Principle

scholarly journals In-Depth Qualitative Analysis of Lime Essential Oils Using the Off-Line Combination of Normal Phase High Performance Liquid Chromatography and Comprehensive Two-Dimensional Gas Chromatography-Quadrupole Mass Spectrometry

Foods ◽  
2019 ◽  
Vol 8 (11) ◽  
pp. 580 ◽  
Author(s):  
Mariosimone Zoccali ◽  
Barbara Giocastro ◽  
Ivana L. Bonaccorsi ◽  
Alessandra Trozzi ◽  
Peter Q. Tranchida ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Essential Oil ◽  
Qualitative Analysis ◽  
High Performance ◽  
Normal Phase ◽  
Two Dimensional ◽  
Quadrupole Mass

The present research is focused on the in-depth qualitative analysis of three types of lime essential oil (EO), viz., Key (A and B) and Persian, using the off-line combination of normal phase high performance liquid chromatography (NP-HPLC) and comprehensive two-dimensional gas chromatography–quadrupole mass spectrometry (GC × GC-QMS). The first analytical dimension (NP-HPLC) was exploited for the isolation of the hydrocarbon constituents from the oxygenated ones. Each fraction was then reduced in volume and analyzed using (cryogenic modulation) GC × GC-QMS. Peak assignment was carried out through the combined use of mass spectral database and linear retention index matching processes. The powerful four-dimensional technology enabled the separation and identification of a very high number (153) of lime essential oil volatile compounds.

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