The Marriage of Protein and Lanthanide: Unveiling a Time-Resolved Fluorescence Sensor Array Regulated by pH toward High-Throughput Assay of Metal Ions in Biofluids

2019 ◽  
Vol 91 (17) ◽  
pp. 11170-11177 ◽  
Author(s):  
Zi-Yang Lin ◽  
Zhi-bei Qu ◽  
Zi-Han Chen ◽  
Xin-Yue Han ◽  
Ling-Xue Deng ◽  
...  
Keyword(s):  
Metal Ions ◽  
High Throughput ◽  
Sensor Array ◽  
Fluorescence Sensor ◽  
Time Resolved Fluorescence ◽  
Time Resolved ◽  
High Throughput Assay

A high-throughput assay for HIV-1 integrase 3′-processing activity using time-resolved fluorescence

2012 ◽  
Vol 184 (1-2) ◽  
pp. 34-40 ◽  
Author(s):  
Ying-Shan Han ◽  
Peter Quashie ◽  
Thibault Mesplede ◽  
Hongtao Xu ◽  
Kevork Mekhssian ◽  
...  
Keyword(s):  
High Throughput ◽  
Time Resolved Fluorescence ◽  
Time Resolved ◽  
Processing Activity ◽  
High Throughput Assay ◽  
Hiv 1

A High-Throughput, Time-Resolved Fluorescence Approach Increases γ-H2AX Detection

2021 ◽  
Author(s):  
Felicite Noubissi ◽  
Amber McBride ◽  
Hannah Leppert ◽  
Larry Millet ◽  
Xiaofei Wang ◽  
...  
Keyword(s):  
Dna Damage ◽  
Fluorescence Detection ◽  
High Throughput ◽  
Strand Break ◽  
Carcinoma Cells ◽  
Time Resolved Fluorescence ◽  
Time Resolved ◽  
Dna Double Strand Break ◽  
User Friendly ◽  
High Throughput Assay

Abstract Phosphorylation of the histone protein H2AX to form γ-H2AX foci directly represents DNA double-strand break formation. Traditional γ-H2AX detection involves counting individual foci within individual nuclei. Here, we present the development of a sensitive high-throughput assay to quantify γ-H2AX using dissociation-enhanced lanthanide fluorescence immunoassay and time-resolved fluorescence. For comparison, standard fluorescence detection was employed and analyzed either by bulk fluorescent measurements or by direct foci counting using BioTek Spot Count algorithm and Gen 5 software. Etoposide induced DNA damage in A549 carcinoma cells was compared across all test platforms. Time resolved fluorescence detection of europium as a chelated complex enabled quantitative measurement of γ-H2AX foci with nanomolar resolution. Comparative bulk fluorescent signals achieved only micromolar sensitivity. Lanthanide based immunodetection of γ-H2AX offers superior detection and a user-friendly workflow. These high throughput approaches can potentially improve screening of compounds that either enhance DNA damage or protect against its deleterious effects.

scholarly journals Fluorescent chemosensors for metal ions based on 3-(2-benzoxazol-5-yl)alanine skeleton

2010 ◽  
Vol 8 (3) ◽  
pp. 674-686 ◽  
Author(s):  
Magda Milewska ◽  
Katarzyna Guzow ◽  
Wiesław Wiczk
Keyword(s):  
Steady State ◽  
Fluorescence Spectroscopy ◽  
Metal Ions ◽  
Ground State ◽  
Transition Metal Ions ◽  
Fluorescent Chemosensors ◽  
Time Resolved Fluorescence ◽  
Absorption Bands ◽  
Time Resolved ◽  
Ratiometric Sensors

AbstractThe ability of new chelate ligands, benzoxazol-5-yl-alanine derivatives substituted in position 2 by heteroaromatic substituent, to form complexes with selected metal ions in acetonitrile are studied by means of absorption and steady-state and time-resolved fluorescence spectroscopy. Among the ligands studied, only azaaromatic derivatives form stable complexes with transition metal ions in the ground state. Their absorption bands are bathochromically shifted enabling to use those ligands as ratiometric sensors. The fluorescence of each ligand is quenched by metal ions, however, in the presence of Cd(II) and Zn(II) ions a new red shifted emission band is observed.

Identification of proteins and bacteria based on a metal ion–gold nanocluster sensor array

2018 ◽  
Vol 10 (32) ◽  
pp. 3939-3944 ◽  
Author(s):  
Yayan Wu ◽  
Bing Wang ◽  
Kai Wang ◽  
Peng Yan
Keyword(s):  
Metal Ions ◽  
Metal Ion ◽  
Sensor Array ◽  
Gold Nanoclusters ◽  
Fluorescence Sensor ◽  
Gold Nanocluster

We report a simple fluorescence sensor array based on metal ions–gold nanoclusters (AuNCs) for the identification of proteins. The proposed method can also be used for bacteria sensing.

Spectroscopic evidence for hydration and dehydration of lipid bilayers upon interaction with metal ions: a new physical insight

2018 ◽  
Vol 20 (21) ◽  
pp. 14796-14807 ◽  
Author(s):  
Soumya Kanti De ◽  
Nishu Kanwa ◽  
Mirajuddin Ahamed ◽  
Anjan Chakraborty
Keyword(s):  
Steady State ◽  
Metal Ions ◽  
Lipid Bilayers ◽  
Phospholipid Bilayers ◽  
Time Resolved Fluorescence ◽  
Time Resolved ◽  
Membrane Probe ◽  
Physical Insight ◽  
Chain Lengths ◽  
Hydration And Dehydration

In this manuscript, we investigate the interactions of different metal ions with zwitterionic phospholipid bilayers of different chain lengths using the well-known membrane probe PRODAN and steady state and time resolved fluorescence spectroscopy.

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