Controlled Cross-Linking with Glucose Oxidase for the Enhancement of Gelling Potential of Pork Myofibrillar Protein

2016 ◽  
Vol 64 (50) ◽  
pp. 9523-9531 ◽  
Author(s):  
Xu Wang ◽  
Youling L. Xiong ◽  
Hiroaki Sato ◽  
Yoshiyuki Kumazawa
Keyword(s):  
Glucose Oxidase ◽  
Myofibrillar Protein ◽  
Cross Linking

Optimal Study of Co-Immobilized GOD/CAT on Cross-Linked Chitosan Microsphere Containing L-Lysine

2011 ◽  
Vol 236-238 ◽  
pp. 926-929 ◽  
Author(s):  
Xiao Hua Zhou ◽  
Ying Li Wang ◽  
Xia Li Su
Keyword(s):  
Enzyme Activity ◽  
Glucose Oxidase ◽  
Half Life ◽  
Immobilized Enzyme ◽  
Activity Ratio ◽  
Cross Linking ◽  
Chitosan Microsphere ◽  
Activity Recovery ◽  
The Cross ◽  
Optimized Conditions

Glucose oxidase (GOD) and catalase (CAT) were co-immobilized on the cross-linked chitosan microsphere containing L-Lysine (CCL) by the method of absorption-crosslink, with cross-linking agent of glutaraldehyde. The optimized conditions of the co-immobilization are as follows: activity ratio of GOD and CAT is 0.78:1, concentration of glutaraldehyde is 0.17mmol/L, and the immobilizing process last for 3 hours. The enzyme activity of co-immobilized enzyme is 15.3U/mg and activity recovery is 50.1%. The half life is 231 days, 2.4 times of immobilized GOD.

Research of Electrochemistry Measuring System Based on Saliva Glucose

2011 ◽  
Vol 301-303 ◽  
pp. 1139-1144
Author(s):  
Li Ying Jiang ◽  
Li Jie Ren ◽  
Qing Hua Chen ◽  
Guang Zhao Cui
Keyword(s):  
Glucose Oxidase ◽  
Chemical Sensors ◽  
Linear Relation ◽  
Glucose Solution ◽  
Solution Concentration ◽  
Measuring System ◽  
Cross Linking ◽  
Glucose Content ◽  
Relationship Of ◽  
The Relationship

According to the relationship of glucose content in diabetes saliva and blood, the biosensor for detecting saliva glucose was introduced based on electrochemical detecting principle, glucose oxidase was immobilized onto the surface of electrode by glutaraldehyde cross-linking. The measuring system was designed by using potentiostat for amperometric chemical sensors. The characters of the microsystem has been demonstrated with the detection of standard glucose solution concentration of saliva parameter. Compared with the data obtained from the instrument CHI660A, it showed that has a good linear relation in the linear range of 0-2200µmol/L, with the correlation coefficient of 0.9531.

scholarly journals 3D Hydrogen Titanate Nanotubes on Ti Foil: A Carrier for Enzymatic Glucose Biosensor

Sensors ◽  
2020 ◽  
Vol 20 (4) ◽  
pp. 1024 ◽  
Author(s):  
Lulu Ma ◽  
Zhao Yue ◽  
Guona Huo ◽  
Shasha Zhang ◽  
Baolin Zhu ◽  
...  
Keyword(s):  
Glucose Oxidase ◽  
Linear Response ◽  
High Selectivity ◽  
3D Structure ◽  
Glucose Biosensor ◽  
Titanate Nanotubes ◽  
Cross Linking ◽  
In Situ Fabrication ◽  
Hydrogen Titanate

Glucose oxidase (GOx) based biosensors are commercialized and marketed for the high selectivity of GOx. Incorporation nanomaterials with GOx can increase the sensitivity performance. In this work, an enzyme glucose biosensor based on nanotubes was fabricated. By using Ti foil as a carrier, hydrogen titanate nanotubes (HTNTs), which present fine 3D structure with vast pores, were fabricated in-situ by the hydrothermal treatment. The multilayer nanotubes are open-ended with a diameter of 10 nm. Then glucose oxidase (GOx) was loaded on the nanotubes by cross-linking to form an electrode of the amperometric glucose biosensor (GOx/HTNTs/Ti electrode). The fabricated GOx/HTNTs/Ti electrode had a linear response to 1–10 mM glucose, and the response time was 1.5 s. The sensitivity of the biosensor was 1.541 μA·mM-1·cm-2, and the detection limit (S/N = 3) was 59 μM. Obtained results indicate that the in-situ fabrication and unique 3D structure of GOx/HTNTs/Ti electrode are beneficial for its sensitivity.

Co-Immobilization of Glucose Oxidase-Catalase: Optimization of Immobilization Parameters to Improve the Immobilization Yield

2011 ◽  
Vol 7 (2) ◽  
Author(s):  
Sandip B Bankar ◽  
Mahesh V Bule ◽  
Rekha S Singhal ◽  
Laxmi A Ananthanarayan
Keyword(s):  
Glucose Oxidase ◽  
Response Surface ◽  
Glass Bead ◽  
Cross Linking ◽  
Optimization Strategy ◽  
Composite Optimization ◽  
The Cross ◽  
Glass Surfaces ◽  
Central Composite ◽  
Potential Use

Co-immobilization of glucose oxidase (EC 1.1.3.4) and catalase (EC 1. 11.1.6) on non-porus glass surfaces using ?-aminopropyltriethoxysilane and polyethyleneimine as an activator and gluteraldehyde as cross linking agent has been carried out for its potential use. Polyethyleneimine was found to be a superior immobilization activator than ?-aminopropyltriethoxysilane. In present study, the effects of rough and smooth beads and optimization of the ratio of enzyme concentrations, activator material and concentration of the cross linking agent were investigated using response surface technology. With optimized concentration of glucose oxidase to catalase ratio (0.97), polyethyleneimine (150 mg/l) and gluteraldehyde (15 ml), the effect of glass bead concentration for maximum immobilization yield was investigated. Central composite optimization strategy with 16 experiments increased immobilization yield from 82.63% to 92.74%. It was observed that 0.3 ml of beads per 120 U of glucose oxidase were necessary for higher immobilization yield.

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