Effects of Medium- and Long-Chain Triacylglycerols on Lipid Metabolism and Gut Microbiota Composition in C57BL/6J Mice

The main aim of the present study was to investigate the effects of dietary trans-10, cis-12-conjugated linoleic acid (t10c12-CLA) on intestinal microbiota composition and SCFA production. C57BL/6 mice (n 8 per group) were fed a standard diet either supplemented with t10c12-CLA (0·5 %, w/w) (intervention) or with no supplementation (control), daily for 8 weeks. Metabolic markers (serum glucose, leptin, insulin and TAG, and liver TAG) were assessed by ELISA commercial kits, tissue long-chain fatty acids and caecal SCFA by GC, and microbial composition by 16S rRNA pyrosequencing. Dietary t10c12-CLA significantly decreased visceral fat mass (P< 0·001), but did not affect body weight (intervention), when compared with no supplementation (control). Additionally, lipid mass and composition were affected by t10c12-CLA intake. Caecal acetate, propionate and isobutyrate concentrations were higher (P< 0·05) in the t10c12-CLA-supplemented group than in the control group. The analysis of the microbiota composition following 8 weeks of t10c12-CLA supplementation revealed lower proportions of Firmicutes (P= 0·003) and higher proportions of Bacteroidetes (P= 0·027) compared with no supplementation. Furthermore, t10c12-CLA supplementation for 8 weeks significantly altered the gut microbiota composition, harbouring higher proportions of Bacteroidetes, including Porphyromonadaceae bacteria previously linked with negative effects on lipid metabolism and induction of hepatic steatosis. These results indicate that the mechanism of dietary t10c12-CLA on lipid metabolism in mice may be, at least, partially mediated by alterations in gut microbiota composition and functionality.

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Ganoderic acid A from Ganoderma lucidum ameliorates lipid metabolism and alters gut microbiota composition in hyperlipidemic mice fed a high-fat diet

Food & Function ◽

10.1039/d0fo00436g ◽

2020 ◽

Vol 11 (8) ◽

pp. 6818-6833

Author(s):

Wei-Ling Guo ◽

Jian-Bin Guo ◽

Bin-Yu Liu ◽

Jin-Qiang Lu ◽

Min Chen ◽

...

Keyword(s):

Lipid Metabolism ◽

Gut Microbiota ◽

High Fat Diet ◽

Ganoderma Lucidum ◽

Metabolite Profile ◽

Microbiota Composition ◽

High Fat ◽

Ganoderic Acid ◽

Gut Microbiota Composition

Ganoderic acid A from Ganoderma lucidum has the potential to prevent hyperlipidemia, modulates the composition of gut microbiota in hyperlipidemic mice, and significantly attenuates the liver metabolite profile in hyperlipidemic mice.

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Antibiotic affects the gut microbiota composition and expression of genes related to lipid metabolism and myofiber types in skeletal muscle of piglets

BMC Veterinary Research ◽

10.1186/s12917-020-02592-0 ◽

2020 ◽

Vol 16 (1) ◽

Author(s):

Honglin Yan ◽

Bing Yu ◽

Jeroen Degroote ◽

Thomas Spranghers ◽

Noémie Van Noten ◽

...

Keyword(s):

Skeletal Muscle ◽

Lipid Metabolism ◽

Gut Microbiota ◽

Antibiotic Administration ◽

Antibiotic Exposure ◽

Microbiota Composition ◽

Muscle Properties ◽

Expression Of Genes ◽

Imf Content ◽

Gut Microbiota Composition

Abstract Background Early-life antibiotic administration is known to affect gut microbiota and host adiposity, but the effects of antibiotic exposure on skeletal muscle properties remain unknown. The present study evaluated the changes in skeletal muscle properties including myofiber characteristics and composition, as well as intramuscular fat (IMF) content in skeletal muscle of piglets when exposed to a tylosin-containing diet. Results A total of 18 piglets (28 days of age) were randomly allocated into two groups: control basal diet (Control) and Control + 100 mg tylosin phosphate/kg of feed (Antibiotic). The trial lasted for 39 days. High-throughput amplicon sequencing revealed that no significant difference in initial gut microbiota composition was existed between Control and Antibiotic groups. Antibiotic administration increased body weight and growth rate and decreased feed to gain ratio of pigs (P < 0.05). The carcass lean and fat volumes of pigs were increased by the tylosin administration (P < 0.05). Antibiotic treatment increased myofiber density and the expression of genes related to type I and type IIb myofibers in longissimus muscle (P < 0.05). The IMF content in longissimus muscle was increased by antibiotic exposure (P < 0.05). Antibiotic administration increased expression of genes related to fatty acid uptake and de novo synthesis, and decreased expression of genes related to triglyceride hydrolysis (P < 0.05). Tylosin administration affected taxonomic distribution and beta diversity of the caecal and colonic microbiota of piglets. Conclusion These results confirm that the growth performance, myofiber composition and muscle lipid metabolism are affected by antibiotic administration, which may be associated with an altered gut microbiota, suggesting that the gut microbiota could be served as a potential target for modulating skeletal muscle properties of host.

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Polyphenolic-enriched peach peels extract regulates lipid metabolism and improves the gut microbiota composition in high fat diet-fed mice

Journal of Functional Foods ◽

10.1016/j.jff.2020.104082 ◽

2020 ◽

Vol 72 ◽

pp. 104082

Author(s):

Juan Kan ◽

Cuicui Chen ◽

Tongbin Huo ◽

Wangjin Xie ◽

Yaoyao Hui ◽

...

Keyword(s):

Lipid Metabolism ◽

Gut Microbiota ◽

High Fat Diet ◽

Microbiota Composition ◽

High Fat ◽

Gut Microbiota Composition

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Hematopoietic Npc1 mutation shifts gut microbiota composition in Ldlr−/− mice on a high-fat, high-cholesterol diet

Scientific Reports ◽

10.1038/s41598-019-51525-x ◽

2019 ◽

Vol 9 (1) ◽

Author(s):

Tom Houben ◽

John Penders ◽

Yvonne Oligschlaeger ◽

Inês A. Magro dos Reis ◽

Marc-Jan Bonder ◽

...

Keyword(s):

Lipid Metabolism ◽

Gut Microbiota ◽

High Cholesterol Diet ◽

Microbiota Composition ◽

High Cholesterol ◽

High Fat ◽

Microbial Composition ◽

Host Genetics ◽

Gut Microbiota Composition

Abstract While the link between diet-induced changes in gut microbiota and lipid metabolism in metabolic syndrome (MetS) has been established, the contribution of host genetics is rather unexplored. As several findings suggested a role for the lysosomal lipid transporter Niemann-Pick type C1 (NPC1) in macrophages during MetS, we here explored whether a hematopoietic Npc1 mutation, induced via bone marrow transplantation, influences gut microbiota composition in low-density lipoprotein receptor knockout (Ldlr−/−) mice fed a high-fat, high-cholesterol (HFC) diet for 12 weeks. Ldlr−/− mice fed a HFC diet mimic a human plasma lipoprotein profile and show features of MetS, providing a model to explore the role of host genetics on gut microbiota under MetS conditions. Fecal samples were used to profile the microbial composition by 16 s ribosomal RNA gene sequencing. The hematopoietic Npc1 mutation shifted the gut microbiota composition and increased microbial richness and diversity. Variations in plasma lipid levels correlated with microbial diversity and richness as well as with several bacterial genera. This study suggests that host genetic influences on lipid metabolism affect the gut microbiome under MetS conditions. Future research investigating the role of host genetics on gut microbiota might therefore lead to identification of diagnostic and therapeutic targets for MetS.

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Effect of Grifola frondosa 95% ethanol extract on lipid metabolism and gut microbiota composition in high-fat diet-fed rats

Food & Function ◽

10.1039/c8fo01116h ◽

2018 ◽

Vol 9 (12) ◽

pp. 6268-6278 ◽

Cited By ~ 12

Author(s):

Yu-Yang Pan ◽

Feng Zeng ◽

Wei-Ling Guo ◽

Tian-Tian Li ◽

Rui-Bo Jia ◽

...

Keyword(s):

Lipid Metabolism ◽

Gut Microbiota ◽

High Fat Diet ◽

Ethanol Extract ◽

Grifola Frondosa ◽

Microbiota Composition ◽

High Fat ◽

Gut Microbiota Composition

This study aimed to investigate the effects of 95% ethanol extract of G. frondosa (GF95) on lipid metabolism and gut microbiota composition in high-fat diet (HFD) fed rats.

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