Polysorbate 20 Degradation in Biopharmaceutical Formulations: Quantification of Free Fatty Acids, Characterization of Particulates, and Insights into the Degradation Mechanism

2015 ◽  
Vol 12 (11) ◽  
pp. 3805-3815 ◽  
Author(s):  
Anthony Tomlinson ◽  
Barthélemy Demeule ◽  
Baiwei Lin ◽  
Sandeep Yadav
Keyword(s):  
Fatty Acids ◽  
Free Fatty Acids ◽  
Degradation Mechanism ◽  
Polysorbate 20

scholarly journals TLC immunostaining characterization of Clostridium botulinum type A neurotoxin binding to gangliosides and free fatty acids

FEBS Letters ◽  
1986 ◽  
Vol 201 (2) ◽  
pp. 229-232 ◽  
Author(s):  
Kotaro Takamizawa ◽  
Masao Iwamori ◽  
Shunji Kozaki ◽  
Genji Sakaguchi ◽  
Ryuichiro Tanaka ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Free Fatty Acids ◽  
Clostridium Botulinum ◽  
Type A ◽  
Botulinum Type

Characterization of Collagen Fiber/BaSO4 Hybrid Materials and Their Applications in Free Fatty Acids Adsorption

2016 ◽  
Vol 10 (4) ◽  
pp. 302-307
Author(s):  
Hu Zhou ◽  
Jie Zhou ◽  
Yizhi Xiong ◽  
Jianxian Zeng ◽  
Zhihua Zhou ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Free Fatty Acids ◽  
Hybrid Materials ◽  
Collagen Fiber

scholarly journals Analysis of fatty acids and triacylglycerides by Pd nanoparticle-assisted laser desorption/ionization mass spectrometry

2015 ◽  
Vol 7 (9) ◽  
pp. 3701-3707 ◽  
Author(s):  
Yuliya E. Silina ◽  
Claudia Fink-Straube ◽  
Heiko Hayen ◽  
Dietrich A. Volmer
Keyword(s):  
Mass Spectrometry ◽  
Fatty Acids ◽  
Free Fatty Acids ◽  
Laser Desorption ◽  
Ionization Mass ◽  
Laser Desorption Ionization ◽  
Pd Nanoparticle ◽  
Rapid Technique ◽  
Desorption Ionization

In this study, we propose a simple and rapid technique for characterization of free fatty acids and triacylglycerides (TAG) based on palladium nanoparticular (Pd-NP) surface-assisted laser desorption/ionization (SALDI) mass spectrometry (MS).

Biochemical characterization of polyunsaturated fatty acid synthesis in Schizochytrium: Release of the products as free fatty acids

2009 ◽  
Vol 47 (6) ◽  
pp. 472-478 ◽  
Author(s):  
James G. Metz ◽  
Jerry Kuner ◽  
Bradley Rosenzweig ◽  
James C. Lippmeier ◽  
Paul Roessler ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Free Fatty Acids ◽  
Polyunsaturated Fatty Acid ◽  
Fatty Acid Synthesis ◽  
Biochemical Characterization ◽  
Acid Synthesis

scholarly journals Secreted Enzymatic Activities of Wild-Type andpilD-Deficient Legionella pneumophila

2000 ◽  
Vol 68 (4) ◽  
pp. 1855-1863 ◽  
Author(s):  
Virginia Aragon ◽  
Sherry Kurtz ◽  
Antje Flieger ◽  
Birgid Neumeister ◽  
Nicholas P. Cianciotto
Keyword(s):  
Fatty Acids ◽  
Free Fatty Acids ◽  
Legionella Pneumophila ◽  
Wild Type ◽  
Intracellular Infection ◽  
Type Iv ◽  
Nitrophenyl Phosphate ◽  
Pilus Biogenesis ◽  
Legionnaires Disease

ABSTRACT Legionella pneumophila, the agent of Legionnaires' disease, is an intracellular pathogen of protozoa and macrophages. Previously, we had determined that the Legionella pilD gene is involved in type IV pilus biogenesis, type II protein secretion, intracellular infection, and virulence. Since the loss of pili and a protease do not account for the infection defect exhibited by apilD-deficient strain, we sought to define other secreted proteins absent in the mutant. Based upon the release ofp-nitrophenol (pNP) from p-nitrophenyl phosphate, acid phosphatase activity was detected in wild-type but not in pilD mutant supernatants. Mutant supernatants also did not release either pNP from p-nitrophenyl caprylate and palmitate or free fatty acid from 1-monopalmitoylglycerol, suggesting that they lack a lipase-like activity. However, since wild-type samples failed to release free fatty acids from 1,2-dipalmitoylglycerol or to cleave a triglyceride derivative, this secreted activity should be viewed as an esterase-monoacylglycerol lipase. The mutant supernatants were defective for both release of free fatty acids from phosphatidylcholine and degradation of RNA, indicating that PilD-negative bacteria lack a secreted phospholipase A (PLA) and nuclease. Finally, wild-type but not mutant supernatants liberated pNP from p-nitrophenylphosphorylcholine (pNPPC). Characterization of a new set of mutants defective for pNPPC-hydrolysis indicated that this wild-type activity is due to a novel enzyme, as opposed to a PLC or another known enzyme. Some, but not all, of these mutants were greatly impaired for intracellular infection, suggesting that a second regulator or processor of the pNPPC hydrolase is critical for L. pneumophila virulence.

Synthesis and Characterization of Different Transition Metal-Alginate Based Heterogeneous Catalyst for Esterification Reaction

2016 ◽  
Vol 709 ◽  
pp. 57-60
Author(s):  
Fei Ling Pua ◽  
Kah Thong Looi ◽  
Shamala Gowri Krishnan ◽  
Sharifah Nabihah
Keyword(s):  
Fatty Acids ◽  
Oleic Acid ◽  
Transition Metal ◽  
Free Fatty Acids ◽  
Heterogeneous Catalyst ◽  
Heterogeneous Catalysts ◽  
Biodiesel Production ◽  
Esterification Reaction ◽  
Homogeneous Catalyst

In recent years, attention has been drawn to produce heterogeneous catalyst to replace homogeneous catalyst in biodiesel industry. This study was focused on the synthesis of three different types of alginate based heterogeneous catalyst (Ferric-alginate, Copper-alginate, and Nickel alginate) and the effect of the catalyst on esterification of oleic acid. Morphology and elemental analysis was conducted to investigate the properties of the catalyst. The new heterogeneous catalysts were used to catalyze the esterification of oleic acid at reaction temperature of 60°C and 2 hours reaction time. Fe-alginate has achieved the highest free fatty acids (FFAs) conversation rate of 82.03%. The results and findings proved that transition metal-alginate heterogeneous catalyst has the potential and ability to esterify the free fatty acids prior biodiesel production from high free fatty acids feedstock.

Characterization of Wax Esters, Triglycerides, and Free Fatty Acids of Follicular Casts

1986 ◽  
Vol 86 (6) ◽  
pp. 700-705 ◽  
Author(s):  
Katrina M Nordstrom ◽  
John N Labows ◽  
Kenneth J McGinley ◽  
James J Leyden
Keyword(s):  
Fatty Acids ◽  
Free Fatty Acids ◽  
Wax Esters

Hydrolytic polysorbate 20 degradation – sensitive detection of free fatty acids in biopharmaceuticals via UPLC-QDa analytics with isolator column

2021 ◽  
pp. 122717
Author(s):  
Dirk-H. Evers ◽  
Stefan Carle ◽  
Daniel Lakatos ◽  
Frank Hämmerling ◽  
Patrick Garidel ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Free Fatty Acids ◽  
Sensitive Detection ◽  
Polysorbate 20
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