Development and characterization of an improved silicic acid-KOH arrestant column for routine quantitative isolation of free fatty acids

1980 ◽  
Vol 57 (12) ◽  
pp. 414-416 ◽  
Author(s):  
A. H. Woo ◽  
R. C. Lindsay
Keyword(s):  
Fatty Acids ◽  
Free Fatty Acids ◽  
Silicic Acid

Improved 63Ni radiochemical assay of free fatty acids in plasma.

1987 ◽  
Vol 33 (1) ◽  
pp. 176-179 ◽  
Author(s):  
H Barash ◽  
S Akov
Keyword(s):  
Fatty Acids ◽  
Free Fatty Acids ◽  
Silicic Acid ◽  
Organic Phase ◽  
Sodium Sulfate ◽  
Anhydrous Sodium Sulfate ◽  
Lipid Phosphorus ◽  
Radiochemical Method ◽  
Centrifugation Step

Abstract We have modified the 63Ni radiochemical method of Ho (Anal Biochem 1970;36:105) for determination of free fatty acids (FFA) in plasma. Extracting 1 or 0.1 mL of plasma with Dole's mixture (J Biol Chem 1960;235:2595) and washing the "heptane" layer with two volumes of isopropanol/water/dilute (0.5 mol/L) H2SO4 (25/25/1, by vol) removes about 90% of the lipid phosphorus from the "heptane" layer without removing any FFA and is more convenient than treatment with silicic acid. The following modifications decrease background radioactivity and improve separation of the organic phase from the water phase containing the uncomplexed 63Ni: (a) use glassware instead of plastic test tubes; (b) evaporate the organic phase to dryness before adding the 63Ni (this removes the isopropanol, which interferes with the 63Ni assay); and (c) add anhydrous sodium sulfate before the final centrifugation step.

scholarly journals TLC immunostaining characterization of Clostridium botulinum type A neurotoxin binding to gangliosides and free fatty acids

FEBS Letters ◽  
1986 ◽  
Vol 201 (2) ◽  
pp. 229-232 ◽  
Author(s):  
Kotaro Takamizawa ◽  
Masao Iwamori ◽  
Shunji Kozaki ◽  
Genji Sakaguchi ◽  
Ryuichiro Tanaka ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Free Fatty Acids ◽  
Clostridium Botulinum ◽  
Type A ◽  
Botulinum Type

Characterization of Collagen Fiber/BaSO4 Hybrid Materials and Their Applications in Free Fatty Acids Adsorption

2016 ◽  
Vol 10 (4) ◽  
pp. 302-307
Author(s):  
Hu Zhou ◽  
Jie Zhou ◽  
Yizhi Xiong ◽  
Jianxian Zeng ◽  
Zhihua Zhou ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Free Fatty Acids ◽  
Hybrid Materials ◽  
Collagen Fiber

scholarly journals Analysis of fatty acids and triacylglycerides by Pd nanoparticle-assisted laser desorption/ionization mass spectrometry

2015 ◽  
Vol 7 (9) ◽  
pp. 3701-3707 ◽  
Author(s):  
Yuliya E. Silina ◽  
Claudia Fink-Straube ◽  
Heiko Hayen ◽  
Dietrich A. Volmer
Keyword(s):  
Mass Spectrometry ◽  
Fatty Acids ◽  
Free Fatty Acids ◽  
Laser Desorption ◽  
Ionization Mass ◽  
Laser Desorption Ionization ◽  
Pd Nanoparticle ◽  
Rapid Technique ◽  
Desorption Ionization

In this study, we propose a simple and rapid technique for characterization of free fatty acids and triacylglycerides (TAG) based on palladium nanoparticular (Pd-NP) surface-assisted laser desorption/ionization (SALDI) mass spectrometry (MS).

Biochemical characterization of polyunsaturated fatty acid synthesis in Schizochytrium: Release of the products as free fatty acids

2009 ◽  
Vol 47 (6) ◽  
pp. 472-478 ◽  
Author(s):  
James G. Metz ◽  
Jerry Kuner ◽  
Bradley Rosenzweig ◽  
James C. Lippmeier ◽  
Paul Roessler ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Free Fatty Acids ◽  
Polyunsaturated Fatty Acid ◽  
Fatty Acid Synthesis ◽  
Biochemical Characterization ◽  
Acid Synthesis

scholarly journals Secreted Enzymatic Activities of Wild-Type andpilD-Deficient Legionella pneumophila

2000 ◽  
Vol 68 (4) ◽  
pp. 1855-1863 ◽  
Author(s):  
Virginia Aragon ◽  
Sherry Kurtz ◽  
Antje Flieger ◽  
Birgid Neumeister ◽  
Nicholas P. Cianciotto
Keyword(s):  
Fatty Acids ◽  
Free Fatty Acids ◽  
Legionella Pneumophila ◽  
Wild Type ◽  
Intracellular Infection ◽  
Type Iv ◽  
Nitrophenyl Phosphate ◽  
Pilus Biogenesis ◽  
Legionnaires Disease

ABSTRACT Legionella pneumophila, the agent of Legionnaires' disease, is an intracellular pathogen of protozoa and macrophages. Previously, we had determined that the Legionella pilD gene is involved in type IV pilus biogenesis, type II protein secretion, intracellular infection, and virulence. Since the loss of pili and a protease do not account for the infection defect exhibited by apilD-deficient strain, we sought to define other secreted proteins absent in the mutant. Based upon the release ofp-nitrophenol (pNP) from p-nitrophenyl phosphate, acid phosphatase activity was detected in wild-type but not in pilD mutant supernatants. Mutant supernatants also did not release either pNP from p-nitrophenyl caprylate and palmitate or free fatty acid from 1-monopalmitoylglycerol, suggesting that they lack a lipase-like activity. However, since wild-type samples failed to release free fatty acids from 1,2-dipalmitoylglycerol or to cleave a triglyceride derivative, this secreted activity should be viewed as an esterase-monoacylglycerol lipase. The mutant supernatants were defective for both release of free fatty acids from phosphatidylcholine and degradation of RNA, indicating that PilD-negative bacteria lack a secreted phospholipase A (PLA) and nuclease. Finally, wild-type but not mutant supernatants liberated pNP from p-nitrophenylphosphorylcholine (pNPPC). Characterization of a new set of mutants defective for pNPPC-hydrolysis indicated that this wild-type activity is due to a novel enzyme, as opposed to a PLC or another known enzyme. Some, but not all, of these mutants were greatly impaired for intracellular infection, suggesting that a second regulator or processor of the pNPPC hydrolase is critical for L. pneumophila virulence.

Synthesis and Characterization of Different Transition Metal-Alginate Based Heterogeneous Catalyst for Esterification Reaction

2016 ◽  
Vol 709 ◽  
pp. 57-60
Author(s):  
Fei Ling Pua ◽  
Kah Thong Looi ◽  
Shamala Gowri Krishnan ◽  
Sharifah Nabihah
Keyword(s):  
Fatty Acids ◽  
Oleic Acid ◽  
Transition Metal ◽  
Free Fatty Acids ◽  
Heterogeneous Catalyst ◽  
Heterogeneous Catalysts ◽  
Biodiesel Production ◽  
Esterification Reaction ◽  
Homogeneous Catalyst

In recent years, attention has been drawn to produce heterogeneous catalyst to replace homogeneous catalyst in biodiesel industry. This study was focused on the synthesis of three different types of alginate based heterogeneous catalyst (Ferric-alginate, Copper-alginate, and Nickel alginate) and the effect of the catalyst on esterification of oleic acid. Morphology and elemental analysis was conducted to investigate the properties of the catalyst. The new heterogeneous catalysts were used to catalyze the esterification of oleic acid at reaction temperature of 60°C and 2 hours reaction time. Fe-alginate has achieved the highest free fatty acids (FFAs) conversation rate of 82.03%. The results and findings proved that transition metal-alginate heterogeneous catalyst has the potential and ability to esterify the free fatty acids prior biodiesel production from high free fatty acids feedstock.

Characterization of Wax Esters, Triglycerides, and Free Fatty Acids of Follicular Casts

1986 ◽  
Vol 86 (6) ◽  
pp. 700-705 ◽  
Author(s):  
Katrina M Nordstrom ◽  
John N Labows ◽  
Kenneth J McGinley ◽  
James J Leyden
Keyword(s):  
Fatty Acids ◽  
Free Fatty Acids ◽  
Wax Esters
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