scholarly journals Combination Therapy with DETA/NO and Clopidogrel Inhibits Metastasis in Murine Mammary Gland Cancer Models via Improved Vasoprotection

2018 ◽  
Vol 15 (11) ◽  
pp. 5277-5290 ◽  
Author(s):  
Kseniia Porshneva ◽  
Diana Papiernik ◽  
Mateusz Psurski ◽  
Marcin Nowak ◽  
Rafał Matkowski ◽  
...  
Keyword(s):  
Mammary Gland ◽  
Combination Therapy ◽  
Cancer Models ◽  
Mammary Gland Cancer

scholarly journals Differential Impact of Calcitriol and Its Analogs on Tumor Stroma in Young and Aged Ovariectomized Mice Bearing 4T1 Mammary Gland Cancer

2020 ◽  
Vol 21 (17) ◽  
pp. 6359 ◽  
Author(s):  
Artur Anisiewicz ◽  
Agata Pawlik ◽  
Beata Filip-Psurska ◽  
Joanna Wietrzyk
Keyword(s):  
Mammary Gland ◽  
Tumor Stroma ◽  
Differential Impact ◽  
Anticancer Properties ◽  
Inflammatory Monocytes ◽  
Ovariectomized Mice ◽  
Expression Of Genes ◽  
Cancer Models ◽  
Mammary Gland Cancer ◽  
Young Mice

(1) Background: Vitamin D compounds (VDC) are extensively studied in the field of anticancer properties, including breast cancer. Previously, we showed that calcitriol and its analogs (PRI-2191 and PRI-2205) stimulate metastasis in 4T1 murine mammary gland cancer models in young mice, whereas the reverse effect was observed in aged ovariectomized (OVX) mice; (2) Methods: We determined the phenotype of monocytes/macrophages using FACS and examined the expression of selected genes and proteins by Real-Time PCR and ELISA; (3) Results: Activities of VDC are accompanied by an increase in the percentage of Ly6Clow anti-inflammatory monocytes in the spleen of young and a decrease in aged OVX mice. Treatment of young mice with VDC resulted in an increase of CCL2 plasma and tumor concentration and Arg1 in tumor. In later stage of tumor progression the expression of genes related to metastasis in lung tissue was decreased or increased, in old OVX or young mice, respectively; (4) Conclusions: Pro- or anti-metastatic effects of calcitriol and its analogs in young or aged OVX mice, respectively, can be attributed to the differences in the effects of VDC on the tumor microenvironment, as a consequence of differences in the immunity status of young and aged mice.

scholarly journals Temporal inhibition of mouse mammary gland cancer metastasis by CORM-A1 and DETA/NO combination therapy

Theranostics ◽  
2019 ◽  
Vol 9 (13) ◽  
pp. 3918-3939 ◽  
Author(s):  
Kseniia Porshneva ◽  
Diana Papiernik ◽  
Mateusz Psurski ◽  
Agnieszka Łupicka-Słowik ◽  
Rafał Matkowski ◽  
...  
Keyword(s):  
Mammary Gland ◽  
Combination Therapy ◽  
Cancer Metastasis ◽  
Mouse Mammary Gland ◽  
Mammary Gland Cancer ◽  
Temporal Inhibition

scholarly journals THE MORBIDITY OF MAMMARY GLAND CANCER OF FEMALE POPULATION OF THE SIBERIAN FEDERAL DISTRICT

2019 ◽  
Vol 61 (3) ◽  
pp. 123-128
Author(s):  
Irina N. Odintsova ◽  
L. F. Pisareva ◽  
O. A. Ananina ◽  
E. V. Panferova
Keyword(s):  
Breast Cancer ◽  
Mammary Gland ◽  
Birth Rate ◽  
Malignant Tumors ◽  
Divorce Rate ◽  
Federal District ◽  
Demographic Characteristics ◽  
Siberian Federal District ◽  
Female Population ◽  
Mammary Gland Cancer

The breast cancer is one of main localizations among malignant tumors in women of the Siberian Federal District. In the structure of morbidity it holds first place with such percentage as 20.4% and index of morbidity makes up to 51.2 per 100 000 of female population. The territories with increased and decreased risk are established. The features of prevalence of disease in a certain degree are conditioned by differences in demographic characteristics of populations. The indices of life-span, birth-rate in fertile age and divorce rate effect the level of morbidity of breast cancer in population.

scholarly journals Shp2 signaling suppresses senescence in Py MT ‐induced mammary gland cancer in mice

2015 ◽  
Vol 34 (11) ◽  
pp. 1493-1508 ◽  
Author(s):  
Linxiang Lan ◽  
Jane D Holland ◽  
Jingjing Qi ◽  
Stefanie Grosskopf ◽  
Regina Vogel ◽  
...  
Keyword(s):  
Mammary Gland ◽  
Mammary Gland Cancer

scholarly journals Calcitriol and Its Analogs Establish the Immunosuppressive Microenvironment That Drives Metastasis in 4T1 Mouse Mammary Gland Cancer

2018 ◽  
Vol 19 (7) ◽  
pp. 2116 ◽  
Author(s):  
Agata Pawlik ◽  
Artur Anisiewicz ◽  
Beata Filip-Psurska ◽  
Marcin Nowak ◽  
Eliza Turlej ◽  
...  
Keyword(s):  
Gene Expression ◽  
Mammary Gland ◽  
Lymph Nodes ◽  
Expression Analysis ◽  
Gene Expression Analysis ◽  
Cancer Metastasis ◽  
Transforming Growth Factor ◽  
Mouse Mammary Gland ◽  
Th2 Response ◽  
Mammary Gland Cancer

In our previous study, calcitriol and its analogs PRI-2191 and PRI-2205 stimulated 4T1 mouse mammary gland cancer metastasis. Therefore, we aimed to analyze the inflammatory response in 4T1-bearing mice treated with these compounds. Gene expression analysis of the splenocytes and regional lymph nodes demonstrated prevalence of the T helper lymphocytes (Th2) response with an increased activity of regulatory T (Treg) lymphocytes in mice treated with these compounds. We also observed an increased number of mature granulocytes and B lymphocytes and a decreased number of TCD4+, TCD4+CD25+, and TCD8+, as well as natural killer (NK) CD335+, cells in the blood of mice treated with calcitriol and its analogs. Among the splenocytes, we observed a significant decrease in NK CD335+ cells and an increase in TCD8+ cells. Calcitriol and its analogs decreased the levels of interleukin (IL)-1β and IL-10 and increased the level of interferon gamma (IFN-γ) in the plasma. In the tumor tissue, they caused an increase in the level of IL-10. Gene expression analysis of lung tissue demonstrated an increased level of osteopontin (Spp1) and transforming growth factor β (TGF-β) mRNA. The expression of Spp1 was also elevated in lymph nodes. Calcitriol and its analogs caused prevalence of tumor-conducive changes in the immune system of 4T1 tumor-bearing mice, despite the induction of some tumor-disadvantageous effects.

scholarly journals Repurposing combination therapy of Voacamine with Vincristine for down regulation of HIF-1α/FASN co-axis and PHD2 activation in ER+ mammary neoplasia

2020 ◽  
Author(s):  
Lakhveer Singh ◽  
Manjari Singh ◽  
Dinesh Kumar ◽  
Mohd. Nazam Ansari ◽  
Abdulaziz S. Saeedan ◽  
...  
Keyword(s):  
Mammary Gland ◽  
Fatty Acid ◽  
Combination Therapy ◽  
Fatty Acid Synthase ◽  
Hypoxia Inducible Factor ◽  
Prolyl Hydroxylase ◽  
Over Expression ◽  
Hypoxia Inducible Factor 1Α ◽  
Gland Carcinoma

Abstract Background The current study was attempted to inquest the role of combination therapy of Voacamine and Vincristine for the prevention of mammary gland carcinoma through prolyl hydroxylase-2 activation. The prolyl hydroxylase‐2 activation leads the downregulation of hypoxia‐inducible factor‐1α and fatty acid synthase. Over expression of hypoxia inducible factor-1α and fatty acid synthase is previously reported in solid tumor of mammary gland. Methods After screening a battery of natural compounds which were similar to vincristine, vocamine was selected as a possible prolyl hydroxylase-2 activator and justify its activity using 7, 12-Dimethylbenz[a]anthracene induced rat model. The combination therapy was evaluated for cardiac toxicity using hemodynamic profile. The angiogenic markers were evaluated using carmine staining. Monotherapy and combination therapy were also evaluated for liver and kidney toxicity through haematoxylin and eosin staining. The combination therapy also delineated the markers of oxidative stress favorably. Afterwards, the disruption of fatty acids was evaluated using gas chromatography. Results The immunoblotting analysis validated that combination therapy has a potential to switch on the prolyl hydroxylase-2 activity and thus initiate proteolytic degradation of hypoxia‐inducible factor‐1α and its consequence effects. The combination therapy also stimulated programmed cell death (apoptosis) in rapidly dividing cancer cells. Conclusion The present study explores the role of voacamine in activation of prolyl hydroxylase-2 which can decrease over expression of hypoxia‐inducible factor‐1α and fatty acid synthase in cells of mammary gland carcinoma.

scholarly journals Repurposing Combination Therapy of Voacamine with Vincristine for Down Regulation of HIF-1α/FASN Co-Axis and PHD2 Activation in ER+ Mammary Neoplasia

2020 ◽  
Author(s):  
Lakhveer Singh ◽  
Manjari Singh ◽  
Mohd. Nazam Ansari ◽  
Abdulaziz S. Saeedan ◽  
Gaurav Kaithwas
Keyword(s):  
Mammary Gland ◽  
Fatty Acid ◽  
Combination Therapy ◽  
Fatty Acid Synthase ◽  
Hypoxia Inducible Factor ◽  
Prolyl Hydroxylase ◽  
Over Expression ◽  
Hypoxia Inducible Factor 1Α ◽  
Gland Carcinoma

Abstract Background: The current study was attempted to inquest the role of combination therapy of Voacamine and Vincristine for the prevention of mammary gland carcinoma through prolyl hydroxylase‐2 activation. The prolyl hydroxylase‐2 activation leads the downregulation of hypoxia‐inducible factor‐1α and fatty acid synthase. Over expression of hypoxia inducible factor-1α and fatty acid synthase is previously reported in solid tumor of mammary gland. Methods: After screening a battery of natural compounds which were similar to vincristine, vocamine was selected as a possible prolyl hydroxylase‐2 activator and justify its activity using 7, 12-Dimethylbenz[a]anthracene induced rat model. The combination therapy was evaluated for cardiac toxicity using hemodynamic profile. The angiogenic markers were evaluated using carmine staining. Monotherapy and combination therapy were also evaluated for liver and kidney toxicity through haematoxylin and eosin staining. The combination therapy also delineated the markers of oxidative stress favorably. Afterwards, the disruption of fatty acids was evaluated using gas chromatography. Results: The immunoblotting analysis validated that combination therapy has a potential to switch on the prolyl hydroxylase‐2 activity and thus initiate proteolytic degradation of hypoxia‐inducible factor‐1α and its consequence effects. The combination therapy also stimulated programmed cell death (apoptosis) in rapidly dividing cancer cells.Conclusion: The present study explores the role of voacamine in activation of prolyl hydroxylase‐2 which can decrease over expression of hypoxia‐inducible factor‐1α and fatty acid synthase in cells of mammary gland carcinoma.

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