Point-of-Care Pathogen Testing Using Photonic Crystals and Machine Vision for Diagnosis of Urinary Tract Infections

Nano Letters ◽  
2021 ◽  
Author(s):  
Haoran Liu ◽  
Zhihao Li ◽  
Ruichen Shen ◽  
Zhiheng Li ◽  
Yanbing Yang ◽  
...  
Keyword(s):  
Urinary Tract ◽  
Photonic Crystals ◽  
Machine Vision ◽  
Urinary Tract Infections ◽  
Point Of Care ◽  
Pathogen Testing ◽  
Tract Infections

scholarly journals A Rapid, point-of-care antibiotic susceptibility test for urinary tract infections

2020 ◽  
Vol 20 ◽  
pp. S77-S78
Author(s):  
V.M.Ambrosi Grappelli ◽  
S. Pastore ◽  
I. Amato ◽  
C.Fede Spicchiale ◽  
M. Carilli ◽  
...  
Keyword(s):  
Urinary Tract ◽  
Urinary Tract Infections ◽  
Antibiotic Susceptibility ◽  
Point Of Care ◽  
Susceptibility Test ◽  
Antibiotic Susceptibility Test ◽  
Tract Infections

scholarly journals Sensitivity of C-Reactive Protein and Procalcitonin Measured by Point-of-Care Tests to Diagnose Urinary Tract Infections in Nursing Home Residents: A Cross-Sectional Study

2020 ◽  
Author(s):  
S D Kuil ◽  
S Hidad ◽  
J C Fischer ◽  
J Harting ◽  
C M P M Hertogh ◽  
...  
Keyword(s):  
Nursing Home ◽  
Urinary Tract ◽  
Sample Size ◽  
Urinary Tract Infections ◽  
Point Of Care ◽  
Nursing Home Residents ◽  
C Reactive Protein ◽  
Reactive Protein ◽  
Tract Infections ◽  
Point Of Care Tests

Abstract Background Diagnosing urinary tract infections (UTIs) in nursing home residents is complex, as specific urinary symptoms are often absent and asymptomatic bacteriuria (ASB) is prevalent. The aim of this study was to assess the sensitivity of blood C-reactive protein (CRP) and procalcitonin (PCT), measured by point-of-care tests (PoCTs), to diagnose UTIs in this setting. Methods Elderly residents (≥65 years old) with a suspected UTI were recruited from psychogeriatric, somatic, or rehabilitation wards across 13 participating nursing homes. CRP and PCT were tested simultaneously in the same study participants. To assess the tests’ sensitivities, a stringent definition of “true” UTI was used that included the presence of symptoms, urinary leucocytes, a positive urine culture, and symptom resolution during antibiotic treatment covering isolated uropathogen(s). The original sample size was 440 suspected UTI episodes, in order to detect a clinically relevant sensitivity of at least 65% when calculated using the matched analysis approach to compare both PoCTs. Results After enrollment of 302 episodes (68.6% of the planned sample size), an unplanned and funder-mandated interim analysis was done, resulting in premature discontinuation of the study for futility. For 247 of 266 eligible episodes, all mandatory items required for the true UTI definition (92.9%) were available. In total, 49 episodes fulfilled our stringent UTI definition (19.8%). The sensitivities of CRP (cut-off, 6.5 mg/L) and PCT (cut-off, 0.025 ng/mL) were 52.3% (95% confidence interval [CI], 36.7–67.5%) and 37.0% (95% CI, 23.2–52.5%), respectively. Conclusions Our results indicate that CRP and PCT are not suitable tests for distinguishing UTI and ASB in nursing home residents. Clinical Trials Registration Netherlands Trial Registry NL6293.

A new device for the prompt diagnosis of urinary tract infections

2014 ◽  
Vol 0 (0) ◽  
Author(s):  
Alyexandra Arienzo ◽  
Francesca Losito ◽  
Giorgia Bottini ◽  
Francesca Romana Priolisi ◽  
Alberto Mari ◽  
...  
Keyword(s):  
Urinary Tract ◽  
Urinary Tract Infections ◽  
Point Of Care ◽  
Bacterial Species ◽  
Microbiological Analysis ◽  
New Device ◽  
Biological Survey ◽  
Precise Diagnosis ◽  
Appropriate Therapy ◽  
Tract Infections

AbstractUrinary tract infections (UTIs) are among the most common infectious diseases. Results obtained from conventional microbiological analysis of urine and antibiotic susceptibility testing are available only after a few days, delaying precise diagnosis and appropriate therapy. Micro Biological Survey (MBS) srl (a spin-off of Roma Tre University, Rome, Italy) has developed and patented an automated colorimetric test for rapid bacterial counting. In a preliminary validation study it was demonstrated that the results obtained with the MBS method are equivalent to the results obtained with conventional culture-based microbiological analysis.In this study, sterile urine samples were artificially contaminated with bacterial species that are most frequently responsible for UTIs. The MBS method was used to evaluate the presence of bacteria and their sensitivity to some of the most commonly used antibiotics in UTIs.The MBS method was able to detect in a few hours the presence or absence of bacteria at clinically significant concentrations (>10The results obtained demonstrate that the MBS point-of-care testing (POCT) device could be developed into a valuable aid for the management of UTIs, possibly addressing more precise diagnosis and appropriate therapy.

scholarly journals “A false sense of confidence” The perceived role of inflammatory point-of-care testing in managing urinary tract infections in Dutch nursing homes: a qualitative study

2020 ◽  
Author(s):  
Sacha Danielle Kuil ◽  
Caroline Schneeberger ◽  
Frank van Leth ◽  
Menno de Jong ◽  
Janneke Harting
Keyword(s):  
Urinary Tract ◽  
Nursing Homes ◽  
Urinary Tract Infections ◽  
Point Of Care ◽  
Inflammatory Marker ◽  
Test Results ◽  
Point Of Care Testing ◽  
False Sense ◽  
Tract Infections ◽  
Specific Symptoms

Abstract Background Diagnosing urinary tract infections (UTI) in nursing home residents is complex, due to frequent non-specific symptomatology and asymptomatic bacteriuria. The objective of this study was to explore health care professionals’ perceptions of the proposed use of inflammatory marker Point-Of-Care Testing (POCT) in this respect. Methods We conducted a qualitative inquiry (2018 - 2019) alongside the multicenter PROGRESS study (NL6293), which assessed the sensitivity of C-reactive protein and procalcitonin POCT in UTI. We used semi-structured face-to-face interviews. The participants were physicians (n=12) and nurses (n=6) from 13 nursing homes in the Netherlands. Most respondents were not familiar with inflammatory marker POCT, while some used POCT for respiratory tract infections. Both the interview guide and the analysis of the interview transcripts were based on the Consolidated Framework for Implementation Research. Results All respondents acknowledged that sufficiently sensitive POCT could decrease diagnostic uncertainty to some extent in residents presenting with non-specific symptoms. They primarily thought that negative test results would rule out UTI and justify withholding antibiotic treatment. Secondly, they described how positive test results could rule in UTI and justify antimicrobial treatment. However, most respondents also expected new diagnostic uncertainties to arise. Firstly, in case of negative test results, they were not sure how to deal with residents’ persisting non-specific symptoms. Secondly, in case of positive test results, they feared overlooking infections other than UTI. These new uncertainties could lead to inappropriate antibiotics use. Therefore, POCT was thought to create a false sense of confidence. Conclusions Our study suggests that inflammatory marker POCT will only improve UTI management in nursing homes to some extent. To realize the expected added value, any implementation of POCT requires thorough guidance to ensure appropriate use. Developing UTI markers with high negative and positive predictive values may offer greater potential to improve UTI management in nursing homes.

scholarly journals Point-of-care tests for urinary tract infections: protocol for a systematic review and meta-analysis of diagnostic test accuracy

BMJ Open ◽  
2020 ◽  
Vol 10 (6) ◽  
pp. e033424
Author(s):  
David Fraile Navarro ◽  
Frank Sullivan ◽  
Amaya Azcoaga-Lorenzo ◽  
Virginia Hernandez Santiago
Keyword(s):  
Systematic Review ◽  
Urinary Tract ◽  
Diagnostic Accuracy ◽  
Urinary Tract Infections ◽  
Point Of Care ◽  
Meta Analysis ◽  
Data Availability ◽  
Antibiotic Prescribing ◽  
Tract Infections ◽  
Point Of Care Tests

IntroductionUrinary tract infections (UTIs) are the second most common type of infection worldwide, accounting for a large number of primary care consultations and antibiotic prescribing. Current diagnosis is based on an empirical approach, relying on symptoms and occasional use of urine dipsticks. The diagnostic reference standard is still urine culture, although it is not routinely recommended for uncomplicated UTIs in the community, due to time to diagnosis (48 hours). Faster point-of-care tests have been developed, but their diagnostic accuracy has not been compared. Our objective is to systematically review and meta-analyse the diagnostic accuracy of currently available point-of-care tests for UTIs.Methods and analysisStudies evaluating the diagnostic accuracy of point-of-care tests for UTIs will be included. PubMed, Web of Science, Embase and Cochrane Database of Systematic Reviews were searched from inception to 1 June 2019. Data extraction and risk-of-bias assessment will be assessed using the Quality Assessment of Diagnostic Accuracy Studies tool. Meta-analysis will be performed depending on data availability and heterogeneity.Ethics and disseminationThis is a systematic review protocol and therefore formal ethical approval is not required, as no primary, identifiable, personal data will be collected. Patients or the public were not involved in the design of our research. However, the findings from this review will be shared with key stakeholders, including patient groups, clinicians and guideline developers, and will also be presented and national and international conferences.PROSPERO registration numberCRD42018112019.

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