scholarly journals A Rapid, point-of-care antibiotic susceptibility test for urinary tract infections

2020 ◽  
Vol 20 ◽  
pp. S77-S78
Author(s):  
V.M.Ambrosi Grappelli ◽  
S. Pastore ◽  
I. Amato ◽  
C.Fede Spicchiale ◽  
M. Carilli ◽  
...  
Keyword(s):  
Urinary Tract ◽  
Urinary Tract Infections ◽  
Antibiotic Susceptibility ◽  
Point Of Care ◽  
Susceptibility Test ◽  
Antibiotic Susceptibility Test ◽  
Tract Infections

scholarly journals A rapid, point-of-care antibiotic susceptibility test for urinary tract infections

2020 ◽  
Vol 19 ◽  
pp. e1290
Author(s):  
V.M. Ambrosi Grappelli ◽  
E. Finazzi Agrò ◽  
I. Amato ◽  
S. Pastore ◽  
M. Carilli ◽  
...  
Keyword(s):  
Urinary Tract ◽  
Urinary Tract Infections ◽  
Antibiotic Susceptibility ◽  
Point Of Care ◽  
Susceptibility Test ◽  
Antibiotic Susceptibility Test ◽  
Tract Infections

scholarly journals Impact of selective reporting of antibiotic susceptibility test results in urinary tract infections in the outpatient setting: a protocol for a pragmatic, prospective quasi-experimental trial

BMJ Open ◽  
2018 ◽  
Vol 8 (11) ◽  
pp. e025810 ◽  
Author(s):  
Francesca Binda ◽  
Sébastien Fougnot ◽  
Patrice De Monchy ◽  
Anne Fagot-Campagna ◽  
Céline Pulcini ◽  
...  
Keyword(s):  
Urinary Tract ◽  
Urinary Tract Infections ◽  
Broad Spectrum ◽  
Antibiotic Susceptibility ◽  
Outpatient Setting ◽  
Susceptibility Test ◽  
Selective Reporting ◽  
Broad Spectrum Antibiotics ◽  
Antibiotic Susceptibility Test ◽  
Tract Infections

IntroductionAntibiotic resistance is a serious and increasing worldwide threat to global public health. One of antibiotic stewardship programmes’ objectives are to reduce inappropriate broad-spectrum antibiotics’ prescription. Selective reporting of antibiotic susceptibility test (AST) results, which consists of reporting to prescribers only few (n=5-6) antibiotics, preferring first-line and narrow-spectrum agents, is one possible strategy advised in recommendations. However, selective reporting of AST has never been evaluated using an experimental design.Methods and analysisThis study is a pragmatic, prospective, multicentre, controlled (selective reporting vs usual complete reporting of AST), before-after (year 2019 vs 2017) study. Selective reporting of AST is scheduled to be implemented from September 2018 in the ATOUTBIO group of 21 laboratories for all Escherichia coli identified in urine cultures in adult outpatients, and to be compared with the usual complete AST performed in the EVOLAB group of 20 laboratories. The main objective is to assess the impact of selective reporting of AST for E. coli-positive urine cultures in the outpatient setting on the prescription of broad-spectrum antibiotics frequently used for urinary tract infections (amoxicillin-clavulanate, third-generation cephalosporins and fluoroquinolones). The primary end point is the after (2019)–before (2017) difference in prescription rates for the previously mentioned antibiotics/classes that will be compared between the two laboratory groups, using linear regression models. Secondary objectives are to evaluate the feasibility of selective reporting of AST implementation by French laboratories and their acceptability by organising focus groups and individual semi-structured interviews with general practitioners and laboratory professionals.Ethics and disseminationThis protocol was approved by French national ethics committees (Comité d’expertise pour les recherches, les études et les évaluations dans le domaine de la santé (TPS 29064) and Commission Nationale de l’Informatique et des Libertés (Décision DR-2018–141)). Findings of this study will be widely disseminated through conference presentations, reports, factsheets and academic publications and generalisation will be further discussed.Trial registration numberNTC03612297.

scholarly journals Bacterial Infection Diagnosis and Antibiotic Prescription in 3 h as an Answer to Antibiotic Resistance: The Case of Urinary Tract Infections

Antibiotics ◽  
2021 ◽  
Vol 10 (10) ◽  
pp. 1168
Author(s):  
Eleonora Nicolai ◽  
Massimo Pieri ◽  
Enrico Gratton ◽  
Guido Motolese ◽  
Sergio Bernardini
Keyword(s):  
Urinary Tract ◽  
Urinary Tract Infections ◽  
Antimicrobial Susceptibility ◽  
Antibiotic Susceptibility ◽  
Clinical Laboratory ◽  
Point Of Care ◽  
Urine Samples ◽  
Clinical Approach ◽  
Antibiotic Susceptibility Test ◽  
Tract Infections

Current methods for the diagnosis of urinary tract infections with antimicrobial susceptibility testing take 2–3 days and require a clinical laboratory. The lack of a rapid, point-of-care antibiotic susceptibility test (AST) has contributed to the misuse of antibiotics when treating urinary tract infections (UTIs) and consequently to the rise of multi-drug-resistant organisms. The current clinical approach has led to reduced treatment options and increased costs of diagnosis and therapy. To address this issue, novel diagnostics are needed for the timely determination of antimicrobial susceptibility. We present a rapid, point-of-care, phenotypic AST device that can report the antibiotic susceptibility/resistance of a uropathogen to a panel of antibiotics in as few as 3 h by utilizing fluorescent-labelling chemistry and a highly sensitive particle-counting instrument. We analysed 744 urine samples from the outpatients and inpatients of two Italian hospitals. The 130 UTI-positive patient urine samples we found were measured using a panel of six common UTI antibiotics plus a growth control. By comparing our results to hospital laboratory urine cultures, we obtained an overall sensitivity = 81%, a specificity = 83%, an SPV (sensitivity predicted value) = 95%, and an RPV (resistance predicted value) = 54%. According to our preliminary data, the sensitivity predicted value for a single antibiotic agent was 95%, thus allowing (in the vast majority of cases) an early (within 3 h) recognition of an effective agent for a single patient.

scholarly journals Antibiotic susceptibility profile and extended spectrum β-lactamases production by uropathogenic Escherichia coli from tertiary care hospital of rural settings

2018 ◽  
Vol 6 (12) ◽  
pp. 4022
Author(s):  
Mustafa Sofiur Rahman ◽  
Ritu Garg ◽  
Varsha A. Singh ◽  
Dipankar Biswas
Keyword(s):  
Escherichia Coli ◽  
Drug Resistance ◽  
Urinary Tract ◽  
Urinary Tract Infections ◽  
Antibiotic Susceptibility ◽  
Uropathogenic Escherichia Coli ◽  
Diffusion Method ◽  
Care Hospital ◽  
Antibiotic Susceptibility Test ◽  
Tract Infections

Background: Escherichia coli are the most common cause of urinary tract infections in community as well as hospital settings. Emergence of drug resistance in Escherichia coli due to various mechanisms makes the treatment options very limited. This study was undertaken to detect ESBLs in uropathogenic Escherichia coli isolates and to determine their antimicrobial susceptibility pattern in rural setting.Methods: A prospective study was done on 502 E. coli isolates from clinically suspected cases of urinary tract infections (UTI) patients of all age groups. All samples were inoculated on Cysteine Lactose Electrolyte Deficient Agar (CLED). Organisms grown in pure culture were identified by standard biochemical tests. Antibiotic susceptibility test was done by the Kirby Bauer Disc diffusion method on Muller Hinton agar. ESBL detection was done as per CLSI guidelines.Results: Of the 502 isolates of Escherichia coli, nitrofurantoin (82%) was found be most sensitive antimicrobial followed by amikacin (73%), gentamycin (71%) and imipenem (64%). Common empirically used antibiotics like fluroquinolones and Cotrimoxazole drugs showed alarming rate of resistance. 60% isolates were found to be multidrug resistant. ESBL production was detected in 31% isolates. ESBL producing strains were found to be more drug resistant than non ESBL producing strains.Conclusions: So, drug resistance due to production of ESBLs in Escherichia coli is a serious threat for clinicians. Strict infection control measures and early detection of beta lactamase producing isolates are the need of the hour to contain the emergence of this type of resistance.

scholarly journals Prevalence and Antibiograms of Uropathogens from the Suspected Cases of Urinary Tract Infections in Bharatpur Hospital, Nepal

2019 ◽  
Vol 15 (4) ◽  
pp. 260-266
Author(s):  
Sanjib Adhikari ◽  
Sujan Khadka ◽  
Sanjeep Sapkota ◽  
Jid Chani Rana ◽  
Santosh Khanal ◽  
...  
Keyword(s):  
Urinary Tract ◽  
Antibiotic Susceptibility ◽  
Susceptibility Test ◽  
Effective Control ◽  
Diffusion Method ◽  
Gram Positive ◽  
Gram Negative ◽  
E Coli ◽  
Antibiotic Susceptibility Test ◽  
Tract Infections

Background: This study was conducted to determine the prevalence and antibiotic susceptibility pattern of the uropathogens among the patients attending Bharatpur Hospital. Methods: A laboratory-based cross-sectional study was carried out among the patients attending Bharatpur Hospital from December 2017 to February 2018. Aseptically collected clean catch mid-stream urine samples from 200 clinically suspected patients were cultured and processed for the identification of the uropathogens in the laboratory using standard microbiological procedures. Antibiotic susceptibility test was performed for all the isolates against commonly used antibiotics using the Kirby-Bauer disc diffusion method according to Clinical and Laboratory Standards Institute guidelines 2017. Results: Out of 200 samples collected, 59(29.5%) of the samples showed the presence of pathogens causing urinary tract infection (UTI). Among them, 43(72.9%) were Gram-negative and 16(27.1%) were Gram-positive bacteria. UTI was found to be the most prevalent in females compared to the males and in the age group of 21-30 years. E. coli (72.0%) and Staphylococcus epidermidis (50.0%) were the most predominant Gram-negative and Gram-positive isolates respectively. The isolates were resistant to cefpodoxime (54.2%) and least resistant to gentamicin (10.2%). Twenty (33.9%) isolates were found to be multi-drug resistant (MDR). Conclusions: Higher frequency of antibiotic resistance among UTI patients alerts for continuous surveillance to assure effective control of this infection. Awareness of good hygienic practice especially in females and prudent use of antibiotics in case of infection can be sug-gested. Keywords: antibiotic susceptibility test; E. coli; MDR; urine.

scholarly journals Predictors of Urinary Tract Infections in Children and Antibiotic Susceptibility Pattern in the Buea Health District, South West Region, Cameroon

2020 ◽  
Vol 2020 ◽  
pp. 1-11
Author(s):  
Che Pantalius Nji ◽  
Jules Clément Nguedia Assob ◽  
Jane-Francis Tatah Kihla Akoachere
Keyword(s):  
Risk Factors ◽  
Urinary Tract ◽  
Urinary Tract Infections ◽  
Antibiotic Susceptibility ◽  
Trichomonas Vaginalis ◽  
Pediatric Population ◽  
Health Facilities ◽  
Bivariate Analysis ◽  
Cross Sectional ◽  
Tract Infections

Urinary tract infections (UTI) are among the most common pediatric infections and if not promptly diagnosed and treated, it could cause long term complications. Worldwide and in Cameroon, little attention has been paid to this growing problem in the pediatric population. Identification of risk factors will contribute significantly to prevention. A cross-sectional case-control study was carried out in children ≤ 15 years to identify the risk factors of UTI, etiologic agents, and their antibiotic susceptibility. Samples (urine) were collected from in and outpatients with symptoms of UTI attending two health facilities in Buea. Controls were age- and sex-matched children in the community and those visiting these health facilities for unrelated reasons. Samples were analyzed by microscopy, culture, and antibiotic susceptibility of bacteria isolates tested by the disc diffusion technique. Questionnaires were administered to collect sociodemographic, clinical characteristics and data on risk factors. Odds ratios and bivariate and multivariate analyses were used to assess the relationship between predictors (symptoms and risk factors) and UTI. P < 0.05 was considered significant. A total of 405 participants (200 cases and 205 controls) were investigated. UTI prevalence was 12% in cases. From the UTI cases, bacteria was the major cause of infection, with E. coli (39.4%) predominating. Parasitic organisms, Trichomonas vaginalis (0.5%) and Schistosoma spp (0.5%), and yeast (6%) were also detected. Urinary urgency ( F = 4.98 , P = 0.027 ) and back pain ( F = 12.37 , P = 0.001 ) were associated to UTI following bivariate analysis. These parameters could be used to predict UTI in the pediatric population in the study area. Third generation cephalosporins: ceftriaxone (90.1%) and cefadroxil (85.4%) were the most effective and thus recommended for treatment.

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