scholarly journals Ultrasensitive and Simultaneous Detection of Two Specific SARS-CoV-2 Antigens in Human Specimens Using Direct/Enrichment Dual-Mode Fluorescence Lateral Flow Immunoassay

2021 ◽  
Vol 13 (34) ◽  
pp. 40342-40353
Author(s):  
Chongwen Wang ◽  
Xiaodan Cheng ◽  
Liyan Liu ◽  
Xiaochang Zhang ◽  
Xingsheng Yang ◽  
...  
Keyword(s):  
Simultaneous Detection ◽  
Lateral Flow ◽  
Lateral Flow Immunoassay ◽  
Dual Mode

scholarly journals Sensitive and Simultaneous Detection of SARS-CoV-2-Specific IgM/IgG Using Lateral Flow Immunoassay Based on Dual-Mode Quantum Dot Nanobeads

2020 ◽  
Vol 92 (23) ◽  
pp. 15542-15549
Author(s):  
Chongwen Wang ◽  
Xingsheng Yang ◽  
Bing Gu ◽  
Haifeng Liu ◽  
Zihui Zhou ◽  
...  
Keyword(s):  
Quantum Dot ◽  
Simultaneous Detection ◽  
Lateral Flow ◽  
Lateral Flow Immunoassay ◽  
Dual Mode

scholarly journals The simultaneous detection of the squamous cell carcinoma antigen and cancer antigen 125 in the cervical cancer serum using nano-Ag polydopamine nanospheres in an SERS-based lateral flow immunoassay

RSC Advances ◽  
2020 ◽  
Vol 10 (49) ◽  
pp. 29156-29170
Author(s):  
Ji Xia ◽  
Yifan Liu ◽  
Menglin Ran ◽  
Wenbo Lu ◽  
Liyan Bi ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Simultaneous Detection ◽  
Lateral Flow ◽  
Lateral Flow Immunoassay ◽  
Squamous Cell Carcinoma Antigen ◽  
Cancer Antigen 125 ◽  
Cancer Antigen

Based on SERS-based lateral flow immunoassay, nano-Ag polydopamine nanospheres was used for detecting squamous cell carcinoma antigen and cancer antigen 125 simultaneously in cervical cancer serum.

scholarly journals Noncompetitive Chromogenic Lateral-Flow Immunoassay for Simultaneous Detection of Microcystins and Nodularin

Biosensors ◽  
2019 ◽  
Vol 9 (2) ◽  
pp. 79 ◽  
Author(s):  
Sultana Akter ◽  
Teemu Kustila ◽  
Janne Leivo ◽  
Gangatharan Muralitharan ◽  
Markus Vehniäinen ◽  
...  
Keyword(s):  
Water Quality ◽  
Simultaneous Detection ◽  
Antibody Fragment ◽  
Water Quality Monitoring ◽  
Lateral Flow ◽  
Cyanobacterial Blooms ◽  
World Health ◽  
Lateral Flow Immunoassay ◽  
Guideline Value ◽  
Health Organization

Cyanobacterial blooms cause local and global health issues by contaminating surface waters. Microcystins and nodularins are cyclic cyanobacterial peptide toxins comprising numerous natural variants. Most of them are potent hepatotoxins, tumor promoters, and at least microcystin-LR is possibly carcinogenic. In drinking water, the World Health Organization (WHO) recommended the provisional guideline value of 1 µg/L for microcystin-LR. For water used for recreational activity, the guidance values for microcystin concentration varies mostly between 4–25 µg/L in different countries. Current immunoassays or lateral flow strips for microcystin/nodularin are based on indirect competitive method, which are generally more prone to sample interference and sometimes hard to interpret compared to two-site immunoassays. Simple, sensitive, and easy to interpret user-friendly methods for first line screening of microcystin/nodularin near water sources are needed for assessment of water quality and safety. We describe the development of a two-site sandwich format lateral-flow assay for the rapid detection of microcystins and nodularin-R. A unique antibody fragment capable of broadly recognizing immunocomplexes consisting of a capture antibody bound to microcystins/nodularin-R was used to develop the simple lateral flow immunoassay. The assay can visually detect the major hepatotoxins (microcystin-LR, -dmLR, -RR, -dmRR, -YR, -LY, -LF -LW, and nodularin-R) at and below the concentration of 4 µg/L. The signal is directly proportional to the concentration of the respective toxin, and the use of alkaline phosphatase activity offers a cost efficient alternative by eliminating the need of toxin conjugates or other labeling system. The easy to interpret assay has the potential to serve as a microcystins/nodularin screening tool for those involved in water quality monitoring such as municipal authorities, researchers, as well as general public concerned of bathing water quality.

A bare-eye-based lateral flow immunoassay based on the use of gold nanoparticles for simultaneous detection of three pesticides

2014 ◽  
Vol 181 (13-14) ◽  
pp. 1565-1572 ◽  
Author(s):  
Limin Wang ◽  
Jia Cai ◽  
Yulong Wang ◽  
Qingkui Fang ◽  
Suyan Wang ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Simultaneous Detection ◽  
Lateral Flow ◽  
Lateral Flow Immunoassay

Gold nanoparticles-based lateral flow immunoassay with silver staining for simultaneous detection of fumonisin B1 and deoxynivalenol

Food Control ◽  
2015 ◽  
Vol 54 ◽  
pp. 347-352 ◽  
Author(s):  
Qing Yu ◽  
Heng Li ◽  
Chenglong Li ◽  
Suxia Zhang ◽  
Jianzhong Shen ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Silver Staining ◽  
Simultaneous Detection ◽  
Fumonisin B1 ◽  
Lateral Flow ◽  
Lateral Flow Immunoassay

scholarly journals Nucleic Acid Lateral Flow Immunoassay for the Detection of Pathogenic Bacteria from Food

2009 ◽  
Vol 27 (Special Issue 1) ◽  
pp. S350-S353 ◽  
Author(s):  
M. Blažková ◽  
M. Koets ◽  
J. H Wichers ◽  
A. van Amerongen ◽  
L. Fukal ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Pathogenic Bacteria ◽  
Simultaneous Detection ◽  
Lateral Flow ◽  
Lateral Flow Immunoassay ◽  
Carbon Particles ◽  
Microbiological Methods ◽  
Biological Principle ◽  
Primer Sets ◽  
Food Bacteria

Nucleic acid lateral flow immunoassay (NALFIA) is a method combining molecular biological principle of detection with immunochemical principle of visualisation. Following isolation of DNA from the sample, a duplex PCR with two primer sets, of which one was labelled with biotin and the other with digoxigenin or fluorescein, respectively, was performed. The PCR solution and carbon particles conjugated with avidin are directly added to the nitrocellulose membrane with two test lines of immobilised antibodies specific for digoxigenin and fluorescein. The appearance of a black line indicates the presence of specific amplicon. We would like to present the NALFIA for the simultaneous detection of L. monocytogenes in particular and the genus Listeria in general, in food. Bacteria from the genus Listeria frequently contaminate a large variety of foods. Occurrence of Listeria strains in food may indicate errors in good hygienic and manufacturing practice, only L. monocytogenes is a significant human and animal pathogen responsible for the serious illness listeriosis. Conventional microbiological methods for L. monocytogenes detection are laborious and take several days to achieve a confirmed identification.

Glycation ratio determination through simultaneous detection of human serum albumin and glycated albumin on an advanced lateral flow immunoassay sensor

Lab on a Chip ◽  
2020 ◽  
Vol 20 (4) ◽  
pp. 844-851 ◽  
Author(s):  
Hangil Ki ◽  
Jusung Oh ◽  
Gyeo-Re Han ◽  
Min-Gon Kim
Keyword(s):  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Simultaneous Determination ◽  
Glycated Albumin ◽  
Simultaneous Detection ◽  
Lateral Flow ◽  
Lateral Flow Immunoassay ◽  
Ratio Determination

We developed two advanced lateral flow immunoassays (LFIAs) for the simultaneous determination of total human serum albumin and glycated albumin concentrations with wide detection ranges and improved glycated albumin selectivity.

Development of a lateral flow immunoassay for the simultaneous detection of four dipyrone metabolites in milk

2019 ◽  
Vol 11 (23) ◽  
pp. 3041-3052 ◽  
Author(s):  
Yue Li ◽  
Liqiang Liu ◽  
Hua Kuang ◽  
Chuanlai Xu
Keyword(s):  
Simultaneous Detection ◽  
Lateral Flow ◽  
Lateral Flow Immunoassay ◽  
Inflammatory Drug ◽  
Anti Inflammatory

Dipyrone (DIP) is a commonly used non-steroidal anti-inflammatory drug with strong analgesic, antipyretic, and spasmolytic properties.

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