scholarly journals Noncompetitive Chromogenic Lateral-Flow Immunoassay for Simultaneous Detection of Microcystins and Nodularin

Biosensors ◽  
2019 ◽  
Vol 9 (2) ◽  
pp. 79 ◽  
Author(s):  
Sultana Akter ◽  
Teemu Kustila ◽  
Janne Leivo ◽  
Gangatharan Muralitharan ◽  
Markus Vehniäinen ◽  
...  
Keyword(s):  
Water Quality ◽  
Simultaneous Detection ◽  
Antibody Fragment ◽  
Water Quality Monitoring ◽  
Lateral Flow ◽  
Cyanobacterial Blooms ◽  
World Health ◽  
Lateral Flow Immunoassay ◽  
Guideline Value ◽  
Health Organization

Cyanobacterial blooms cause local and global health issues by contaminating surface waters. Microcystins and nodularins are cyclic cyanobacterial peptide toxins comprising numerous natural variants. Most of them are potent hepatotoxins, tumor promoters, and at least microcystin-LR is possibly carcinogenic. In drinking water, the World Health Organization (WHO) recommended the provisional guideline value of 1 µg/L for microcystin-LR. For water used for recreational activity, the guidance values for microcystin concentration varies mostly between 4–25 µg/L in different countries. Current immunoassays or lateral flow strips for microcystin/nodularin are based on indirect competitive method, which are generally more prone to sample interference and sometimes hard to interpret compared to two-site immunoassays. Simple, sensitive, and easy to interpret user-friendly methods for first line screening of microcystin/nodularin near water sources are needed for assessment of water quality and safety. We describe the development of a two-site sandwich format lateral-flow assay for the rapid detection of microcystins and nodularin-R. A unique antibody fragment capable of broadly recognizing immunocomplexes consisting of a capture antibody bound to microcystins/nodularin-R was used to develop the simple lateral flow immunoassay. The assay can visually detect the major hepatotoxins (microcystin-LR, -dmLR, -RR, -dmRR, -YR, -LY, -LF -LW, and nodularin-R) at and below the concentration of 4 µg/L. The signal is directly proportional to the concentration of the respective toxin, and the use of alkaline phosphatase activity offers a cost efficient alternative by eliminating the need of toxin conjugates or other labeling system. The easy to interpret assay has the potential to serve as a microcystins/nodularin screening tool for those involved in water quality monitoring such as municipal authorities, researchers, as well as general public concerned of bathing water quality.

scholarly journals Temporal and spatial variability of cyanobacterial toxins microcystins in three interconnected freshwater reservoirs

2010 ◽  
Vol 75 (9) ◽  
pp. 1303-1312 ◽  
Author(s):  
Luděk Bláha ◽  
Lucie Bláhová ◽  
Jiří Kohoutek ◽  
Ondřej Adamovský ◽  
Pavel Babica ◽  
...  
Keyword(s):  
Dry Weight ◽  
Cyanobacterial Blooms ◽  
World Health ◽  
Cyanobacterial Toxins ◽  
Toxic Cyanobacteria ◽  
Guideline Value ◽  
High Concentrations ◽  
Health Organization ◽  
Reported Data ◽  
Natural Dynamics

In spite of substantial research on health and the ecological risks associated with cyanobacterial toxins in the past decades, the understanding of the natural dynamics and variability of toxic cyanobacterial blooms is still limited. Herein, the results of long term monitoring 1998-1999 / 2001-2008 of three reservoirs (V?r, Brno and Nov? Ml?ny), where toxic blooms develop annually, are reported. These three reservoirs provide a unique model because they are interconnected by the Svratka River, which allows possible transfer of phytoplankton as well as toxins from one reservoir to another. The frequency of the occurrence and dominance of the major cyanobacterial taxa Microcystis aeruginosa did not change during the investigated period but substantial variability was observed in the composition of other phytoplankton. Although absolute concentrations of the studied toxins (microcystins) differed among the reservoirs, there were apparent parallel trends. For example, during certain years, the microcystin concentrations were systematically elevated in all three studied reservoirs. Furthermore, the concentration profiles in the three sites were also correlated (parallel trends) within individual seasons based on monthly sampling. Microcystin-LR, a variant for which the World Health Organization has recommended a guideline value, formed only about 30-50% of the total microcystins. This is of importance, especially in the V?r reservoir that serves as a drinking water supply. Maxima in the cell-bound microcystins (intracellular; expressed per dry weight biomass) generally preceded the maxima of total microcystins (expressed per volume of water sample). Overall, the maximum concentration in the biomass (all three reservoirs, period 1993-2005) was 6.1 mg g-1 dry weight and the median values were in the range 0.065-2.3 mg g-1 dry weight. These are generally high concentrations in comparison with both Czech Republic and worldwide reported data. The present data revealed substantial variability of both toxic cyanobacteria and their peptide toxins that should be reflected by detailed monitoring programs.

scholarly journals A Summer of Cyanobacterial Blooms in Belgian Waterbodies: Microcystin Quantification and Molecular Characterizations

Toxins ◽  
2022 ◽  
Vol 14 (1) ◽  
pp. 61
Author(s):  
Wannes Hugo R. Van Hassel ◽  
Mirjana Andjelkovic ◽  
Benoit Durieu ◽  
Viviana Almanza Marroquin ◽  
Julien Masquelier ◽  
...  
Keyword(s):  
Water Samples ◽  
Direct Sequencing ◽  
Amplicon Sequencing ◽  
Cyanobacterial Blooms ◽  
World Health ◽  
Limit Of Quantification ◽  
Guideline Value ◽  
16S Rna ◽  
Mcye Gene ◽  
Health Organization

In the context of increasing occurrences of toxic cyanobacterial blooms worldwide, their monitoring in Belgium is currently performed by regional environmental agencies (in two of three regions) using different protocols and is restricted to some selected recreational ponds and lakes. Therefore, a global assessment based on the comparison of existing datasets is not possible. For this study, 79 water samples from a monitoring of five lakes in Wallonia and occasional blooms in Flanders and Brussels, including a canal, were analyzed. A Liquid Chromatography with tandem mass spectrometry (LC-MS/MS) method allowed to detect and quantify eight microcystin congeners. The mcyE gene was detected using PCR, while dominant cyanobacterial species were identified using 16S RNA amplification and direct sequencing. The cyanobacterial diversity for two water samples was characterized with amplicon sequencing. Microcystins were detected above limit of quantification (LOQ) in 68 water samples, and the World Health Organization (WHO) recommended guideline value for microcystins in recreational water (24 µg L−1) was surpassed in 18 samples. The microcystin concentrations ranged from 0.11 µg L−1 to 2798.81 µg L−1 total microcystin. For 45 samples, the dominance of the genera Microcystis sp., Dolichospermum sp., Aphanizomenon sp., Cyanobium/Synechococcus sp., Planktothrix sp., Romeria sp., Cyanodictyon sp., and Phormidium sp. was shown. Moreover, the mcyE gene was detected in 75.71% of all the water samples.

scholarly journals Assessment of Household Water Quality from Selected Storage Vessels used in a South-Western Rural Community, Nigeria

2021 ◽  
pp. 31-44
Author(s):  
David Bamidele Olawade ◽  
Ojima Zechariah Wada ◽  
Omotayo Asogbon ◽  
Grace O. Owojori ◽  
Adesina Olufemi Adewole
Keyword(s):  
Water Quality ◽  
Drinking Water ◽  
Anthropogenic Activities ◽  
Water Quality Monitoring ◽  
World Health ◽  
Household Water ◽  
Quality Water ◽  
Health Organization ◽  
Plastic Containers ◽  
Clay Pots

Water is an important requirement for life but its contamination via natural and anthropogenic activities is of great concern. This study determined some physicochemical parameters of drinking water from the main source (borehole), and selected storage vessels in Abimbola, Ayedaade Local Government Area, Osun State, Nigeria. About 10 household water samples were collected randomly from the 70 households in the village. The major water storage vessels used by the villagers were clay pots. Only about one-fifth of the households used plastic containers. All the physico-chemical parameters assessed were within permissible limits of the World Health Organization and Standards Organization of Nigeria’s drinking water guidelines except for Lead and nitrite. Furthermore, water stored in clay pots had significantly higher levels of Nitrate (p=0.04), Nitrite (p=0.04), Sulphate (p=0.04), Lead (p=0.03), Iron (p=0.04), and Manganese (p=0.04) than those stored in plastic containers. Results suggest that the type of storage vessels used could influence the physicochemical quality of the water stored in them. Also, basic water quality monitoring needs to be conducted routinely to ascertain and maintain high quality water supply per time.

Drinking-Water Quality and Intestinal Infectious Diseases in a Colombian Department, 2010-2016

2020 ◽  
pp. 21-29
Author(s):  
Dora Cardona Rivas ◽  
Militza Yulain Cardona Guzmán ◽  
Olga Lucía Ocampo López
Keyword(s):  
Water Quality ◽  
Drinking Water ◽  
Infectious Diseases ◽  
Disease Burden ◽  
Social Protection ◽  
Drinking Water Quality ◽  
World Health ◽  
The Social ◽  
Etiological Agents ◽  
Health Organization

Objective: To characterize the burden of intestinal infectious diseases attributable to drinking-water quality in 27 municipalities in the central region of Colombia. Materials and methods: A time-trend ecological study. The drinking-water quality of the National Institute of Health and the Institute of Hydrology, Meteorology and Environmental Studies was identified. The disease burden was calculated based on the mortality registered in the National Department of Statistics and the records of morbidity attended by the Social Protection Integrated Information System. The etiological agents reported in morbidity records and the observation of environmental conditions in the municipalities of the study were included. The disease burden was determined according to the methodology recommended by the World Health Organization (WHO).

scholarly journals Assessment of drinking water contamination in food stalls of Jashore Municipality, Bangladesh

2021 ◽  
Vol 11 (8) ◽  
Author(s):  
Molla Rahman Shaibur ◽  
Mohammed Sadid Hossain ◽  
Shirina Khatun ◽  
F. K. Sayema Tanzia
Keyword(s):  
Water Quality ◽  
Drinking Water ◽  
World Health Organization ◽  
Fast Food ◽  
Pathogenic Bacteria ◽  
Permissible Limit ◽  
World Health ◽  
Drinking Water Contamination ◽  
The World ◽  
Health Organization

AbstractThis study aimed to determine the quality of drinking water supplied in different types of food stalls in Jashore Municipality, Bangladesh. A total of 35 water samples were collected from different tea stalls, street side fast food stalls, normal restaurants and well-furnished restaurants. The water quality was evaluated by determining the distinct physical, chemical and biological parameters. The results revealed that the water used in the food stalls and restaurants for drinking purpose was in desired quality in terms of turbidity, electrical conductivity, pH, total dissolved solids, nitrate (NO3−), sulfate (SO42−), phosphate (PO43−), chloride (Cl−), sodium (Na) and potassium (K) concentrations. The values were within the permissible limit proposed by the Bangladesh Bureau of Statistics and the World Health Organization. Concentrations of calcium (Ca) and magnesium (Mg) found in several samples were higher than the World Health Organization standard. Iron (Fe) concentrations were higher than the permissible limit of the World Health Organization. Only 46% exceeded the permissible limit of Bangladesh Bureau Statistics. The threatening result was that the samples were contaminated by fecal coliform, indicating that the people of Jashore Municipality may have a greater chance of being affected by pathogenic bacteria. The drinking water provided in the street side fast food stalls was biologically contaminated. The findings demonstrate that the drinking water used in food stalls and restaurants of Jashore Municipality did not meet up the potable drinking water quality standards and therefore was detrimental to public health.

scholarly journals Classification Maps for TDS Concentrations in the GIS Along Euphrates River, Iraq

2021 ◽  
Vol 232 (8) ◽  
Author(s):  
Ali Chabuk ◽  
Zahraa Ali Hammood ◽  
Nadhir Al-Ansari ◽  
Salwan Ali Abed ◽  
Jan Laue
Keyword(s):  
Water Quality ◽  
Drinking Water ◽  
World Health Organization ◽  
World Health ◽  
Euphrates River ◽  
Poor Water Quality ◽  
Quality Category ◽  
Health Organization ◽  
Dissolved Salts

AbstractIraq currently undergoing the problem of water shortage, although Iraq has two Rivers (Euphrates and Tigris) pass throughout most of its areas, and they have represented a major source of water supply. In the current research, to evaluate the quality of the Euphrates river in Iraq based on the values of total dissolved salts (TDS), the TDS concentrations were collected from sixteen sections along the river in the three succeeding years (2011, 2012, and 2013). The evaluation of the river was done depending on the classification of (W.H.O. (World Health Organization). (2003). Total Dissolved Salts in Drinking-water: Background document for development of W.H.O. Guidelines for Drinking-water Quality. World Health Organization, 20 Avenue Appia, 1211 Geneva 27, Switzerland). of rivers for drinking uses. Inverse Distance Weighting Technique (IDWT) as a tool in the GIS was employed to establish the maps of the river that using interpolation/prediction for the TDS concentrations to each selected year and the average values of TDS for these 3 years. Based on the five categories of rivers’ classification of the TDS concentrations according to the (W.H.O. (World Health Organization). (2003). Total Dissolved Salts in Drinking-water: Background document for development of W.H.O. Guidelines for Drinking-water Quality. World Health Organization, 20 Avenue Appia, 1211 Geneva 27, Switzerland), the Euphrates river was classified, and the maps of classification for the years 2011, 2012 and 2013 and the average values for 3 years were created. The average values for 3 years of TDS along the Euphrates river indicated that the sections from SC-1 to SC-4 as moderate-water-quality-Category-3, the sections from SC-5 to SC-10 as poor-water-quality-Category-4, while the sections between SC-11 to SC-16 as very poor-water-quality-Category-5. The interpolation maps showed that the Euphrates river in Iraq was ranged from moderate water quality (Category-3) to very poor water quality (Category-5).

Physico-chemical and Biological Properties of Groundwater Quality in Rural Settlement, Nadi, Fiji

2021 ◽  
Vol 18 (1) ◽  
pp. 1-6
Author(s):  
Roselyn Naidu ◽  
Lionel Joseph ◽  
Syed Sauban Ghani
Keyword(s):  
Water Quality ◽  
Drinking Water ◽  
Biological Properties ◽  
Drinking Water Quality ◽  
World Health ◽  
Wet Season ◽  
Potential Health ◽  
Drinking Water Contamination ◽  
Contamination Levels ◽  
Health Organization

The current study investigated drinking water quality of samples taken from Arolevu village, a locality situated in Nadi, Fiji. The groundwater samples were collected and subjected to a comprehensive physicochemical and biological analysis. The analysis for the drinking water sample was conducted seasonally, six times a year, that is, three for the dry season and three for the wet season. The results retrieved from the analysis were compared to its maximum contamination levels (MCLs) based on the health-based guidelines provided by the World Health Organization (WHO). The WHO standards were used as an attribute to determine the sources of contaminants likely to be present at the study site. A degradation trend in drinking water quality in the context of climate change may lead to potential health impacts. Hence, it is important to understand seasonal variations in drinking water quality. A proper understanding of the drinking water quality through seasonal water analysis for nitrate, nitrite, potassium, calcium, magnesium and chlorine content as well as its microbiological presence to reduce preventable risks such as using calculated amounts of fertilisers and upgrading the sewerage system to alleviate drinking water contamination is devised through this study.

scholarly journals Evaluation of Groundwater Quality in the Vicinity of Khurrianwala Industrial Zone, Pakistan

Water ◽  
2018 ◽  
Vol 10 (10) ◽  
pp. 1321 ◽  
Author(s):  
Muhammad Aleem ◽  
Cao Shun ◽  
Chao Li ◽  
Arslan Aslam ◽  
Wu Yang ◽  
...  
Keyword(s):  
Water Quality ◽  
Groundwater Quality ◽  
Anthropogenic Activities ◽  
World Health ◽  
Total Hardness ◽  
Industrial Zone ◽  
High Concentration ◽  
Physico Chemical ◽  
Health Organization ◽  
National Environmental

The industrial augmentation and unguided anthropogenic activities contaminate water sources in most parts of the world especially in developing countries like Pakistan. High concentration of pollutants in groundwater affects human, soil, and crop health badly. The present study was conducted to investigate groundwater quality for drinking and irrigation purposes in an industrial zone of Pakistan. A GIS tool was used to investigate the spatial distribution of different physico-chemical parameters. In this study, the average results exceeding World Health Organization (WHO) and National Environmental Quality Standards (NEQS) were found for pH 7.84, total dissolved solids (TDS) 1492 mg/L, phosphate 0.51 mg/L, dissolved oxygen (DO) 9.92% saturation, F-coli 6.48 colonies/100 mL, Na+ 366 mg/L, HCO3− 771 mg/L, sulfate 251 mg/L, chlorides 427 mg/L, total hardness (as CaCO3) 292 mg/L, electrical conductivity (EC) 2408 μS/cm, iron (Fe) 0.48 mg/L, chrome (Cr) 0.50 mg/L, arsenic (As) 0.04 mg/L, total phosphorus (TP) 0.17 mg/L, sodium adsorption ratio (SAR) 9.76 (in meq/L), residual sodium carbonate (RSC) 9.28 meq/L, % ion balance 14.4 (in meq/L), percentage sodium ion (% Na+) concentration 58.9 meq/L, and water quality index (WQI) 69.0. The trend of cations and anions were (in meq/L) Na > Mg > Ca > K and HCO3 > Cl > CO3 > SO4 respectively. Although the results of the present study showed poor conditions of the groundwater for drinking as WQI but and irrigation purposes as SAR, it needs to improve some more conditions for the provision of safe drinking water and irrigation water quality.

scholarly journals Effects of Water Quality and Post-Harvest Handling on Microbiological Contamination of Lettuce at Urban and Peri-Urban Locations of Ouagadougou, Burkina Faso

Foods ◽  
2018 ◽  
Vol 7 (12) ◽  
pp. 206 ◽  
Author(s):  
Juliane Dao ◽  
Kathrin Stenchly ◽  
Oumar Traoré ◽  
Philip Amoah ◽  
Andreas Buerkert
Keyword(s):  
Water Quality ◽  
Irrigation Water ◽  
World Health ◽  
Vegetable Production ◽  
Salmonella Spp ◽  
Total Coliforms ◽  
E Coli ◽  
Post Harvest ◽  
Health Organization ◽  
Production Areas

Vegetable production in urban gardens of Ouagadougou contributes to food security, but water for irrigation is often of low quality. This is particularly acute if irrigation water is taken from wastewater polluted channels. This study aimed at (i) verifying to what degree irrigation water quality is correlated with contamination of lettuce with Escherichia coli, total coliforms, and Salmonella spp., and (ii) assessing effects of post-harvest handling on pathogen development during the trade chain. We tested pathogen removal efficiency on lettuce by applying post-harvest washing. Irrigation water of production areas in Ouagadougou (n = 10) showed a mean E. coli load of 2.1 × 105 CFU 100 mL−1. In 60% of the cases, irrigation water did not meet the standards of the World Health Organization (WHO) for safe irrigation water, and in 30% of the cases, irrigation water was contaminated with Salmonella spp. Loads of total coliforms on lettuce leaves ranged from 2.9 × 103 CFU g−1 to 1.3 × 106 CFU g−1, while E. coli averaged 1.1 × 102 CFU g−1. Results on post-harvest handling revealed that microbial loads increased along the trade chain. Overall, half of all lettuce samples (n = 60) were tested positively for Salmonella spp. The experiment showed that appropriate post-harvest handling could prevent the increase of total coliforms.

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