Spatial Presentation of Tissue-Specific Extracellular Matrix Components along Electrospun Scaffolds for Tissue Engineering the Bone–Ligament Interface

2020 ◽  
Vol 6 (9) ◽  
pp. 5145-5161
Author(s):  
Dinorath Olvera ◽  
Binulal N. Sathy ◽  
Daniel J. Kelly
Keyword(s):  
Tissue Engineering ◽  
Extracellular Matrix ◽  
Tissue Specific ◽  
Electrospun Scaffolds ◽  
Extracellular Matrix Components ◽  
Spatial Presentation ◽  
Matrix Components

scholarly journals A Strategy to Enhance Secretion of Extracellular Matrix Components by Stem Cells: Relevance to Tissue Engineering

2018 ◽  
Vol 24 (1-2) ◽  
pp. 145-156 ◽  
Author(s):  
Navaneethakrishnan Krishnamoorthy ◽  
Yuan‐Tsan Tseng ◽  
Poornima Gajendrarao ◽  
Padmini Sarathchandra ◽  
Ann McCormack ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Extracellular Matrix ◽  
Extracellular Matrix Components ◽  
Matrix Components

scholarly journals Comparison of two methods for prolong storage of decellularized mouse whole testis for tissue engineering application: An experimental study

2021 ◽  
Author(s):  
Nasrin Majidi Gharenaz ◽  
Mansoureh Movahedin ◽  
Zohreh Mazaheri
Keyword(s):  
Tissue Engineering ◽  
Experimental Study ◽  
Extracellular Matrix ◽  
Mouse Embryonic Fibroblast ◽  
Slow Freezing ◽  
Histological Evaluation ◽  
Biological Scaffolds ◽  
Extracellular Matrix Components ◽  
Matrix Components ◽  
Embryonic Fibroblast

Background: Biological scaffolds are derived by the decellularization of tissues or organs. Various biological scaffolds, such as scaffolds for the liver, lung, esophagus, dermis, and human testicles, have been produced. Their application in tissue engineering has created the need for cryopreservation processes to store these scaffolds. Objective: The aim was to compare the two methods for prolong storage testicular scaffolds. Materials and Methods: In this experimental study, 20 male NMRI mice (8 wk) were sacrificed and their testes were removed and treated with 0.5% sodium dodecyl sulfate followed by Triton X-100 0.5%. The efficiency of decellularization was determined by histology and DNA quantification. Testicular scaffolds were stored in phosphate-buffered saline solution at 4ºC or cryopreserved by programmed slow freezing followed by storage in liquid nitrogen. Masson’s trichrome staining, Alcian blue staining and immunohistochemistry, collagen assay, and glycosaminoglycan assay were done prior to and after six months of storage under each condition. Results: Hematoxylin-eosin staining showed no remnant cells after the completion of decellularization. DNA content analysis indicated that approximately 98% of the DNA was removed from the tissue (p = 0.02). Histological evaluation confirmed the preservation of extracellular matrix components in the fresh and frozen-thawed scaffolds. Extracellular matrix components were decreased by 4ºC-stored scaffolds. Cytotoxicity tests with mouse embryonic fibroblast showed that the scaffolds were biocompatible and did not have a harmful effect on the proliferation of mouse embryonic fibroblast cells. Conclusion: Our results demonstrated the superiority of the slow freezing method for prolong storage of testicular scaffolds. Key words: Cryopreservation, Testis, Scaffold, Mouse. 

A single-component hydrogel bioink for bioprinting of bioengineered 3D constructs for dermal tissue engineering

2018 ◽  
Vol 5 (6) ◽  
pp. 1100-1111 ◽  
Author(s):  
Rúben F. Pereira ◽  
Aureliana Sousa ◽  
Cristina C. Barrias ◽  
Paulo J. Bártolo ◽  
Pedro L. Granja
Keyword(s):  
Tissue Engineering ◽  
Extracellular Matrix ◽  
De Novo ◽  
Single Component ◽  
Biophysical Properties ◽  
Dermal Tissue ◽  
Extracellular Matrix Components ◽  
Matrix Components

Bioprinted dual-crosslinked 3D constructs with tunable biochemical and biophysical properties guide the de novo deposition of extracellular matrix components of dermal tissue.

scholarly journals In Vitro Generation of Novel Functionalized Biomaterials for Use in Oral and Dental Regenerative Medicine Applications

Materials ◽  
2020 ◽  
Vol 13 (7) ◽  
pp. 1692 ◽  
Author(s):  
Cristina Blanco-Elices ◽  
Enrique España-Guerrero ◽  
Miguel Mateu-Sanz ◽  
David Sánchez-Porras ◽  
Óscar García-García ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Extracellular Matrix ◽  
Regenerative Medicine ◽  
Oral Mucosa ◽  
Dental Tissues ◽  
Extracellular Matrix Components ◽  
Matrix Components ◽  
Time Required ◽  
Vitro Development

Recent advances in tissue engineering offer innovative clinical alternatives in dentistry and regenerative medicine. Tissue engineering combines human cells with compatible biomaterials to induce tissue regeneration. Shortening the fabrication time of biomaterials used in tissue engineering will contribute to treatment improvement, and biomaterial functionalization can be exploited to enhance scaffold properties. In this work, we have tested an alternative biofabrication method by directly including human oral mucosa tissue explants within the biomaterial for the generation of human bioengineered mouth and dental tissues for use in tissue engineering. To achieve this, acellular fibrin–agarose scaffolds (AFAS), non-functionalized fibrin-agarose oral mucosa stroma substitutes (n-FAOM), and novel functionalized fibrin-agarose oral mucosa stroma substitutes (F-FAOM) were developed and analyzed after 1, 2, and 3 weeks of in vitro development to determine extracellular matrix components as compared to native oral mucosa controls by using histochemistry and immunohistochemistry. Results demonstrate that functionalization speeds up the biofabrication method and contributes to improve the biomimetic characteristics of the scaffold in terms of extracellular matrix components and reduce the time required for in vitro tissue development.

scholarly journals Age related extracellular matrix and interstitial cell phenotype in pulmonary valves

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Shaohua Wu ◽  
Vikas Kumar ◽  
Peng Xiao ◽  
Mitchell Kuss ◽  
Jung Yul Lim ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Extracellular Matrix ◽  
Heart Valve ◽  
Cell Phenotype ◽  
Primary Concern ◽  
Ross Operation ◽  
Age Related ◽  
Cardiovascular Morbidity And Mortality ◽  
Extracellular Matrix Components ◽  
Matrix Components

AbstractHeart valve disease is a common manifestation of cardiovascular disease and is a significant cause of cardiovascular morbidity and mortality worldwide. The pulmonary valve (PV) is of primary concern because of its involvement in common congenital heart defects, and the PV is usually the site for prosthetic replacement following a Ross operation. Although effects of age on valve matrix components and mechanical properties for aortic and mitral valves have been studied, very little is known about the age-related alterations that occur in the PV. In this study, we isolated PV leaflets from porcine hearts in different age groups (~ 4–6 months, denoted as young versus ~ 2 years, denoted as adult) and studied the effects of age on PV leaflet thickness, extracellular matrix components, and mechanical properties. We also conducted proteomics and RNA sequencing to investigate the global changes of PV leaflets and passage zero PV interstitial cells in their protein and gene levels. We found that the size, thickness, elastic modulus, and ultimate stress in both the radial and circumferential directions and the collagen of PV leaflets increased from young to adult age, while the ultimate strain and amount of glycosaminoglycans decreased when age increased. Young and adult PV had both similar and distinct protein and gene expression patterns that are related to their inherent physiological properties. These findings are important for us to better understand the physiological microenvironments of PV leaflet and valve cells for correctively engineering age-specific heart valve tissues.

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