scholarly journals Plasmalogen Biosynthesis by Anaerobic Bacteria: Identification of a Two-Gene Operon Responsible for Plasmalogen Production in Clostridium perfringens

2020 ◽  
Author(s):  
David R. Jackson ◽  
Chelsi D. Cassilly ◽  
Damian R. Plichta ◽  
Hera Vlamakis ◽  
Hualan Liu ◽  
...  
Keyword(s):  
Clostridium Perfringens ◽  
Anaerobic Bacteria ◽  
Bacteria Identification

scholarly journals Clostridium botulinum and Clostridium perfringens Occurrence in Kazakh Honey Samples

Toxins ◽  
2019 ◽  
Vol 11 (8) ◽  
pp. 472 ◽  
Author(s):  
Balgabay Maikanov ◽  
Raikhan Mustafina ◽  
Laura Auteleyeva ◽  
Jan Wiśniewski ◽  
Krzysztof Anusz ◽  
...  
Keyword(s):  
Sequence Analysis ◽  
Multiplex Pcr ◽  
Clostridium Perfringens ◽  
Clostridium Botulinum ◽  
Anaerobic Bacteria ◽  
The Other ◽  
Pcr Analysis ◽  
Rdna Genes ◽  
Toxin Sequence ◽  
East Kazakhstan

The aim of this study was to assess occurrence of Clostridium botulinum and Clostridium perfringens in honey samples from Kazakhstan. Analyses were carried out using a set of PCR methods for identification of anaerobic bacteria, and detection of toxin genes of C. botulinum and C. perfringens. Among 197 samples, C. botulinum was noticed in only one (0.5%). The isolated strain of this pathogen showed the presence of the bont/A and ntnh genes. C. perfringens strains were isolated from 18 (9%) samples, and mPCR (multiplex PCR) analysis led to them all being classified as toxin type A with the ability to produce α toxin. Sequence analysis of 16S rDNA genes showed occurrence in 4 samples of other anaerobes related to C. botulinum, which were C. sporogenes and C. beijerinckii strains. C. botulinum prevalence in honey samples from Kazakhstan in comparison to the prevalence in samples collected from the other regions seems to be less. The highest prevalence of Clostridium sp. was noticed in the East Kazakhstan province. Our study is the first survey on BoNT-producing clostridia and C. perfringens prevalence in Kazakh honey.

THE BIOLOGICAL PURIFICATION OF VACCINE EMULSIONS BY PENICILLIN

1946 ◽  
Vol 24e (6) ◽  
pp. 149-154 ◽  
Author(s):  
J. Edouard Morin ◽  
Hector Turcotte
Keyword(s):  
Clostridium Perfringens ◽  
Anaerobic Bacteria ◽  
Vaccine Virus ◽  
Smallpox Vaccine ◽  
New Method ◽  
Present Communication ◽  
Gram Negative ◽  
Biological Purification ◽  
Pathogenic Microbes ◽  
Gram Positive Cocci

The present communication furnishes a new method for the purification of smallpox vaccine emulsion by penicillin. Penicillin, when added in definite quantities, possesses an undeniable antibiotic power on most of the Gram-positive cocci and on the vegetative forms of certain anaerobic bacteria such as Clostridium perfringens and C. fallax found in the vaccine. By maintaining a threshold of penicillin (56 units per cc.), the spores of the above mentioned anaerobic microbes cannot grow. When the vaccine emulsion freed from penicillin is inoculated, new strains without any pathogenic properties can be subcultured. Penicillin has no effect on the vaccine virus. By this technique, the vaccine can be freed, in a few days, of its principal pathogenic microbes except from Gram-negative bacilli. In the emulsion thus treated, the periodic control of the penicillin titre is as necessary as the repeated tests made to establish the potency of the vaccine.

scholarly journals The Comparison of Antibacterial Activity Between Cresophene and Garlic on Isolated of Bacteria in The Necrotic Deciduous Tooth

2020 ◽  
Vol 4 (4) ◽  
pp. 287
Author(s):  
Nurliana ◽  
I Bramanti ◽  
ISR Sudarso ◽  
MSH Wahyunigsih ◽  
T Wibawa
Keyword(s):  
Antibacterial Activity ◽  
Serratia Marcescens ◽  
Clostridium Perfringens ◽  
Anaerobic Bacteria ◽  
Inhibition Zone ◽  
Garlic Extract ◽  
In Vitro Test ◽  
Quantitative Measurements ◽  
Prevotella Bivia

Background : This study aims to compare the ability of two medicaments between cresophene and garlic on antibacterial activity of isolate deciduous necrotic teeth.Methods: In vitro test to see the antibacterial activity was carried out on the aerobic and anaerobic bacteria taken straight from the necrotic tooth of pediatric patients (aged 4-7 years old) that treated for endodontics in Pediatric Dental Clinic of RSGMUGM Prof Soedomo. Bacterial identification using the CLSI Standardized Method 2012 and Biochemical identification by Thermo Scientific Rapid System 2010 guideline. In this identification, was founded three species: Prevotella bivia, Serratia marcescens and Clostridium innocum. This test was carried out quantitative measurements to see the inhibition zone of bacterial growth. Whether cresophene has antibacterial potential test results from using an analytical descriptive test that was tabulated.Study group treated with cresophene in a well of 0.7 cm at a dose of 75 μL, garlic extract at a dose of 75 μL divided into five groups with concentrations of 20%, 40%, 60%, 80%, and garlic filtrate. Comparison of the cresophene and garlic effect conducted in vitro by looking at the inhibition zone of the bacterial growth.Results: In all groups, there are inhibitory zones. Cresophene against bacteria Prevotella bivia (54mm), Clostridium innocum (30 mm), Clostridium perfringens (26 mm), Serratia macescens (26 mm), S.mutans (54 mm), while garlic extract against bacteria Prevotella bivia, Clostridium innocum, Clostridium perfringens, S.mutansdonot show againt inhibitory, Serratia macescens (10mm, in concentration of 80 %) and garlic filtrate against bacteria Prevotella bivia (51 mm), Clostridium innocum (28 mm), Clostridium perfringens (46 mm), Serratia marcescens (31 mm), S.mutans (49 mm).Conclusion: Cresophene has a greater inhibition than garlic extract, but it is lower than garlic filtrate.International Journal of Human and Health Sciences Vol. 04 No. 04 October’20 Page : 287-290

scholarly journals COMPARISON OF ANTIBACTERIAL ACTIVITY OF HONEY AGAINST AEROBIC AND ANAEROBIC BACTERIA

2021 ◽  
pp. 41-43
Author(s):  
S. DHANARAJ ◽  
S. S. M. UMAMAGESWARI ◽  
M. MALAVIKA ◽  
G. BHUVANESHWARI
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Clostridium Perfringens ◽  
Anaerobic Bacteria ◽  
The Other ◽  
E Coli ◽  
Diffusion Technique ◽  
Aerobic And Anaerobic

Objective: To compare the antibacterial activity of honey against aerobic and anaerobic bacteria. Methods: Honey is extracted from the honey comb by trained persons. Antimicrobial activity of honey is performed by Agar Cup Diffusion technique for 3 bacteria Staphylococcus aureus, E. coli and Clostridium perfringens. Results: By performing the technique with proper guidance, it is observed that the Staphylococcus aureus specimen shows sensitivity to honey whereas the other two specimens Escherichia coli and clostridium perfringens doesn’t show any sensitivity to honey. Conclusion: Due to its vast antibacterial activity of honey, it can be used along with other antibiotics to increase its efficiency.

scholarly journals COMPARISON OF ANTIBACTERIAL ACTIVITY OF GARLIC AGAINST AEROBIC AND ANAEROBIC BACTERIA

2021 ◽  
pp. 44-46
Author(s):  
K. G. DHANUSH ◽  
S. S. M. UMAMAGESWARI ◽  
M. MALAVIKA ◽  
G. BHUVANESHWARI
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Clostridium Perfringens ◽  
Anaerobic Bacteria ◽  
The Other ◽  
E Coli ◽  
Diffusion Technique ◽  
Aerobic And Anaerobic

Objective: To compare the antibacterial activity of garlic against aerobic and anaerobic bacteria. Methods: Antimicrobial activity of garlic is performed by Agar cup diffusion technique for 3 bacteria Staphylococcus aureus, E. coli and clostridium perfringens. Results: By performing the technique with proper guidance, it is observed that the Staphylococcus aureus specimen shows sensitivity to garlic whereas the other two specimens Escherichia coli and clostridium perfringens doesn’t show any sensitivity to garlic. Conclusion: Due to its vast antibacterial activity of garlic, it can be used along with other antibiotics to increase its efficiency.

scholarly journals Reduction of bilirubin ditaurate by the intestinal bacterium Clostridium perfringens.

2012 ◽  
Vol 59 (2) ◽  
Author(s):  
Renata Koníčková ◽  
Alena Jirásková ◽  
Jaroslav Zelenka ◽  
Ladislav Lešetický ◽  
Martin Štícha ◽  
...  
Keyword(s):  
Clostridium Perfringens ◽  
Anaerobic Bacteria ◽  
Bile Pigment ◽  
Intestinal Bacterium ◽  
Anaerobic Conditions ◽  
Human Gut ◽  
Lower Vertebrates ◽  
Rf Values ◽  
Hydrolysis Of

Bilirubin is degraded in the human gut by microflora into urobilinoids. In our study we investigated whether the bilirubin-reducing strain of Clostridium perfringens can reduce bilirubin ditaurate (BDT), a bile pigment of some lower vertebrates, without hydrolysis of the taurine moiety. C. perfringes was incubated under anaerobic conditions with BDT; reduction products were quantified by spectrophotometry and separated by TLC. Based on Rf values of BDT reduction products and synthetic urobilinogen ditaurate, three novel taurine-conjugated urobilinoids were identified. It is likely that bilirubin-reducing enzyme(s) serve for the effective disposal of electrons produced by fermentolytic processes in these anaerobic bacteria.

scholarly journals ANTIBACTERIAL EFFECTIVENESS OF PROSOPIS JULIFLORA AGAINST AEROBIC AND ANAEROBIC BACTERIA

2020 ◽  
pp. 110-113
Author(s):  
AISHWARYA V. ◽  
BHUVANESHWARI G. ◽  
J. R. LILLY GRACE ◽  
ROSY VENNILA
Keyword(s):  
Staphylococcus Aureus ◽  
Clostridium Perfringens ◽  
Extraction Method ◽  
Leaf Extract ◽  
Anaerobic Bacteria ◽  
Agar Plate ◽  
Prosopis Juliflora ◽  
Water Extraction ◽  
Diffusion Method ◽  
Aerobic And Anaerobic

Objective: The aim of this study is to analyse the antibacterial effectiveness of the leaf extract of Prosopis juliflora against aerobic and anaerobic bacteria and the objectives include maintaining the culture of Clostridium perfringens ATCC 3624, Staphylococcus aureus ATCC 25923, E. coli ATCC 25922 and to obtain an extract from the leaves of Prosopis juliflora by water extraction method. Agar cup diffusion testing is carried out to test the effectiveness of the extracted herb against the isolated strains of the above-mentioned bacteria. Methods: Cross-sectional study was conducted for 6 mo in the Clinical Microbiology Laboratory at SMCH. Extract from the leaves of Prosopis juliflora was collected by a water extraction method. Isolation of Clostridium perfringens (from RCMB), Staphylococcus aureus and Escherichia coli were done, following which the agar cup diffusion method was performed in which the collected extracts were placed as discs in the agar plate which had been inoculated with the strains of the isolated bacteria. The agar plate was then incubated anaerobically. Agar cup diffusion method is considered as a primary method to determine the antimicrobial effectiveness of the bacteria. Results: The leaf extract of Prosopis juliflora was found to be effective against both aerobic and anaerobic bacterial strains. Conclusion: Based on the work, the effectiveness of Prosopis juliflora was determined and the mechanism of susceptibility was identified. With future research works in the field, many such natural herbal extracts can be proven to be effective against many pathogenic microorganisms.

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