Real-Time Monitoring of NDM-1 Activity in Live Bacterial Cells by Isothermal Titration Calorimetry: A New Approach To Measure Inhibition of Antibiotic-Resistant Bacteria

2018 ◽  
Vol 4 (12) ◽  
pp. 1671-1678 ◽  
Author(s):  
Yue-Juan Zhang ◽  
Wen-Ming Wang ◽  
Peter Oelschlaeger ◽  
Cheng Chen ◽  
Jin-E Lei ◽  
...  
Keyword(s):  
Real Time ◽  
Isothermal Titration Calorimetry ◽  
Titration Calorimetry ◽  
Resistant Bacteria ◽  
Bacterial Cells ◽  
Real Time Monitoring ◽  
Antibiotic Resistant Bacteria ◽  
Antibiotic Resistant ◽  
New Approach ◽  
Live Bacterial Cells

Real-time monitoring and inhibition of the activity of carbapenemase in live bacterial cells: application to screening of β-lactamase inhibitors

2020 ◽  
Vol 44 (46) ◽  
pp. 20334-20340
Author(s):  
Han Gao ◽  
Ying Ge ◽  
Min-Hao Jiang ◽  
Cheng Chen ◽  
Le-Yun Sun ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Real Time ◽  
Bacterial Cells ◽  
Real Time Monitoring ◽  
Live Bacterial Cells

Antibiotic resistance mediated by β-lactamases including metallo-β-lactamases (MβLs) has become an emerging threat.

Synergy of action of antimicrobial peptides PG-1 and ChBac3.4 with antiseptics against antibiotic-resistant bacteria

2018 ◽  
Vol 18 (4) ◽  
pp. 47-57
Author(s):  
Maria Sergeyevna Zharkova ◽  
Ekaterina S. Umnyakova ◽  
Anna G. Afinogenova ◽  
Gennady E. Afinogenov ◽  
Aleksandr A. Kolobov ◽  
...  
Keyword(s):  
Antimicrobial Peptides ◽  
Sodium Hypochlorite ◽  
Hemolytic Activity ◽  
Etidronic Acid ◽  
Resistant Bacteria ◽  
Bacterial Cells ◽  
Antibiotic Resistant Bacteria ◽  
Antibiotic Resistant ◽  
Bacterial Membranes ◽  
Combined Action

We investigated the combined effects of antimicrobial peptides PG-1 and ChBac3.4 with antiseptics (sodium hypochlorite, dioxidine, prontosan, poviargolum, and etidronic acid) to identify combinations that display synergistic antimicrobial activity against antibiotic-resistant bacteria. We used the checker-board titration method to calculate fractional inhibitory concentration indices, and based on the indices the type of combined action was determined. The combined effect on the metabolic activity of bacteria was evaluated using the fluorescent marker resazurin, and the effect on the permeability of bacterial membranes for chromogenic markers was studied spectrophotometrically. The combined hemolytic activity of the combinations was investigated. Sodium hypochlorite was shown to be antagonistic with both antimicrobial peptides. With other antiseptics, combined action was characterized by additivity or synergy. Synergy was most pronounced with the preparation of highly dispersed silver poviargolum. Antiseptics accelerate the development of the antimicrobial effect of antimicrobial peptides but do not significantly affect the dynamics of the membranolytic action of antimicrobial peptides on bacterial cells. Synergy of hemolytic activity is rare. Thus, the combined use of antimicrobial peptides and antiseptics is promising for combating antibiotic-resistant bacteria and can be used to reduce the toxic effects of these compounds.

Real-time monitoring of D-Ala-D-Ala dipeptidase activity of VanX in living bacteria by isothermal titration calorimetry

2019 ◽  
Vol 578 ◽  
pp. 29-35 ◽  
Author(s):  
Miao Lv ◽  
Yue-Juan Zhang ◽  
Fan Zhou ◽  
Ying Ge ◽  
Mu-Han Zhao ◽  
...  
Keyword(s):  
Real Time ◽  
Isothermal Titration Calorimetry ◽  
Titration Calorimetry ◽  
Real Time Monitoring ◽  
Dipeptidase Activity ◽  
Living Bacteria

scholarly journals Real-Time Monitoring of Membrane-Protein Reconstitution by Isothermal Titration Calorimetry

2013 ◽  
Vol 86 (1) ◽  
pp. 920-927 ◽  
Author(s):  
Nadin Jahnke ◽  
Oxana O. Krylova ◽  
Torben Hoomann ◽  
Carolyn Vargas ◽  
Sebastian Fiedler ◽  
...  
Keyword(s):  
Membrane Protein ◽  
Real Time ◽  
Isothermal Titration Calorimetry ◽  
Titration Calorimetry ◽  
Real Time Monitoring ◽  
Membrane Protein Reconstitution ◽  
Protein Reconstitution

scholarly journals Dielectrophoresis assisted rapid, selective and single cell detection of antibiotic resistant bacteria with G-FETs

2019 ◽  
Author(s):  
Narendra Kumar ◽  
Wenjian Wang ◽  
Juan C. Ortiz-Marquez ◽  
Matthew Catalano ◽  
Mason Gray ◽  
...  
Keyword(s):  
Single Cell ◽  
Pathogenic Bacteria ◽  
Field Effect Transistors ◽  
Bacterial Species ◽  
Resistant Bacteria ◽  
Cell Detection ◽  
Bacterial Cells ◽  
Antibiotic Resistant Bacteria ◽  
Antibiotic Resistant ◽  
Single Cell Detection

AbstractThe rapid increase in antibiotic resistant pathogenic bacteria has become a global threat, which besides the development of new drugs, requires rapid, cheap, scalable, and accurate diagnostics. Label free biosensors relying on electrochemical, mechanical, and mass based detection of whole bacterial cells have attempted to meet these requirements. However, the trade-off between selectivity and sensitivity of such sensors remains a key challenge. In particular, point-of-care diagnostics that are able to reduce and/or prevent unneeded antibiotic prescriptions require highly specific probes with sensitive and accurate transducers that can be miniaturized and multiplexed, and that are easy to operate and cheap. Towards achieving this goal, we present a number of advances in the use of graphene field effect transistors (G-FET) including the first use of peptide probes to electrically detect antibiotic resistant bacteria in a highly specific manner. In addition, we dramatically reduce the needed concentration for detection by employing dielectrophoresis for the first time in a G-FET, allowing us to monitor changes in the Dirac point due to individual bacterial cells. Specifically, we realized rapid binding of bacterial cells to a G-FET by electrical field guiding to the device to realize an overall 3 order of magnitude decrease in cell-concentration enabling a single-cell detection limit, and 9-fold reduction in needed time to 5 minutes. Utilizing our new biosensor and procedures, we demonstrate the first selective, electrical detection of the pathogenic bacterial species Staphylococcus aureus and antibiotic resistant Acinetobacter baumannii on a single platform.

scholarly journals Resistencia a antibióticos: esquivando balas mágicas

2019 ◽  
Author(s):  
Álvaro San Millán
Keyword(s):  
Public Health ◽  
Antibiotic Resistance ◽  
Clinical Practice ◽  
Real Time ◽  
Cause Of Death ◽  
Biological System ◽  
System Innovation ◽  
Resistant Bacteria ◽  
Antibiotic Resistant Bacteria ◽  
Antibiotic Resistant

Antibiotic-resistant bacteria currently represent one of the main public health problems and recent predictions indicate that they will soon become the world’s leading cause of death. The ill-fated journey from the introduction of antibiotics into clinical practice to the current threat of a post-antibiotic era has run its course in just a few decades. Thus, the evolution of antibiotic resistance is probably the most spectacular example of evolution of a biological system innovation that we have had the opportunity to observe in real time. This text discusses some of the evolutionary and molecular keys that have allowed bacteria to go down this path.

scholarly journals Exogenous Citrulline and Glutamine Contribute to Reverse the Resistance of Salmonella to Apramycin

2021 ◽  
Vol 12 ◽  
Author(s):  
Yan Yong ◽  
Yanhong Zhou ◽  
Kexin Liu ◽  
Guochang Liu ◽  
Liqin Wu ◽  
...  
Keyword(s):  
Bacterial Resistance ◽  
Tricarboxylic Acid Cycle ◽  
Animal Health ◽  
Resistant Bacteria ◽  
Drug Resistant ◽  
Bacterial Cells ◽  
Antibiotic Resistant Bacteria ◽  
Antibiotic Resistant ◽  
Drug Resistant Bacteria

Antibiotic resistance is an increasing concern for human and animal health worldwide. Recently, the concept of reverting bacterial resistance by changing the metabolic state of antibiotic-resistant bacteria has emerged. In this study, we investigated the reversal of Apramycin resistance in Salmonella. First, non-targeted metabonomics were used to identify key differential metabolites of drug-resistant bacteria. Then, the reversal effect of exogenous substances was verified in vivo and in vitro. Finally, the underlying mechanism was studied. The results showed that the metabolites citrulline and glutamine were significantly reduced in Apramycin-resistant Salmonella. When citrulline and glutamine were added to the culture medium of drug-resistant Salmonella, the killing effect of Apramycin was restored markedly. Mechanistic studies showed that citrulline and glutamine promoted the Tricarboxylic acid cycle, produced more NADH in the bacteria, and increased the proton-motive force, thus promoting Apramycin entry into the bacterial cells, and killing the drug-resistant bacteria. This study provides a useful method to manage infections by antibiotic-resistant bacteria.

Sign in / Sign up

Export Citation Format

Share Document