Measurement of Absolute Concentration at the Subcellular Scale

ACS Nano ◽  
2020 ◽  
Vol 14 (6) ◽  
pp. 6414-6419
Author(s):  
Brittney L. Gorman ◽  
Melanie A. Brunet ◽  
Susan N. Pham ◽  
Mary L. Kraft
Author(s):  
John C. Russ ◽  
Nicholas C. Barbi

The rapid growth of interest in attaching energy-dispersive x-ray analysis systems to transmission electron microscopes has centered largely on microanalysis of biological specimens. These are frequently either embedded in plastic or supported by an organic film, which is of great importance as regards stability under the beam since it provides thermal and electrical conductivity from the specimen to the grid.Unfortunately, the supporting medium also produces continuum x-radiation or Bremsstrahlung, which is added to the x-ray spectrum from the sample. It is not difficult to separate the characteristic peaks from the elements in the specimen from the total continuum background, but sometimes it is also necessary to separate the continuum due to the sample from that due to the support. For instance, it is possible to compute relative elemental concentrations in the sample, without standards, based on the relative net characteristic elemental intensities without regard to background; but to calculate absolute concentration, it is necessary to use the background signal itself as a measure of the total excited specimen mass.


Author(s):  
P. Echlin ◽  
M. McKoon ◽  
E.S. Taylor ◽  
C.E. Thomas ◽  
K.L. Maloney ◽  
...  

Although sections of frozen salt solutions have been used as standards for x-ray microanalysis, such solutions are less useful when analysed in the bulk form. They are poor thermal and electrical conductors and severe phase separation occurs during the cooling process. Following a suggestion by Whitecross et al we have made up a series of salt solutions containing a small amount of graphite to improve the sample conductivity. In addition, we have incorporated a polymer to ensure the formation of microcrystalline ice and a consequent homogenity of salt dispersion within the frozen matrix. The mixtures have been used to standardize the analytical procedures applied to frozen hydrated bulk specimens based on the peak/background analytical method and to measure the absolute concentration of elements in developing roots.


Author(s):  
K. Barmak

Generally, processing of thin films involves several annealing steps in addition to the deposition step. During the annealing steps, diffusion, transformations and reactions take place. In this paper, examples of the use of TEM and AEM for ex situ and in situ studies of reactions and phase transformations in thin films will be presented.The ex situ studies were carried out on Nb/Al multilayer thin films annealed to different stages of reaction. Figure 1 shows a multilayer with dNb = 383 and dAl = 117 nm annealed at 750°C for 4 hours. As can be seen in the micrograph, there are four phases, Nb/Nb3-xAl/Nb2-xAl/NbAl3, present in the film at this stage of the reaction. The composition of each of the four regions marked 1-4 was obtained by EDX analysis. The absolute concentration in each region could not be determined due to the lack of thickness and geometry parameters that were required to make the necessary absorption and fluorescence corrections.


1995 ◽  
Vol 32 (4) ◽  
pp. 77-88 ◽  
Author(s):  
H. Horn ◽  
D. Uhlmann

Since 1975, different patterns of the growth of Fragilaria and Cyanobacteria in the mid or late summer have been observed in the Saidenbach Reservoir. In most of the years, there was a mutual exclusion of mass growths of these two groups. High yields of Fragilaria caused low yields of blue-greens and vice versa. In the first years, Fragilaria was always the first to achieve a mass growth, followed by the Cyanobacteria. Then, in the last years, the blue-greens were succeeded by Fragilaria. Only in some years, there was a large and simultaneous growth of both groups. It has been shown, that the supply of silicon and phosphorus immediately before controlled the pattern of succession in the midsummer period. Mostly, the Si:P ratio was the regulating factor, but there were also years in which the absolute concentration level of these two nutrients were either non-limiting high or below the minimum resource concentration necessary to compensate for the losses. In these cases, the Si:P ratio lost its controlling function. Other influencing factors are discussed, in particular the effect of turbulent mixing. The special nutrient conditions in the midsummer were not only determined by the external load but also by the Si depletion during the spring mass development of diatoms other than Fragilaria, which mostly is physically controlled. Therefore, the succession pattern in the midsummer is sensitive to the meteorological conditions to a large extent.


1970 ◽  
Vol 48 (5) ◽  
pp. 1067-1072 ◽  
Author(s):  
J. W. T. Dandy

Locomotory activity of brook trout was measured at 10 °C in water of three different oxygen concentrations. Activity was sensed with a thermistor probe in a Wheatstone bridge circuit. Voltage output of the bridge was integrated automatically and recorded.Exposure to 6.0 and 1.7 p.p.m. oxygen induced tin activity response within minutes which reached a peak during the first hour then slowly waned. The intensity of response was greater to lower than to higher oxygen levels. Not all fish responded to 8.0 p.p.m. The response threshold concentration was estimated to be 8.3 p.p.m. oxygen. The response appeared to be made to the absolute concentration rather than to the rate of oxygen decrease.Various difficulties in estimating thresholds for dissolved substances in a constant flow system are considered. It is suggested that the response threshold found here be applied only in the specific circumstances of this experiment.


1984 ◽  
Vol 219 (3) ◽  
pp. 1049-1052 ◽  
Author(s):  
P H Stothart

The apparent partial specific volume, phi, of a protein (or other macromolecule) and the absolute concentration, c, can be determined from the densimetry of solutions and diffusates in 1H2O, 2H2O and 1H2O/2H2O mixtures if the ratios of concentrations (relative concentrations) are known. The densimetry method allows a non-destructive check on total macromolecule concentrations without prior knowledge of specific u.v.-absorption coefficient. The method may be especially useful in small-angle neutron-scattering studies.


2019 ◽  
Vol 316 (3) ◽  
pp. R189-R198 ◽  
Author(s):  
Christopher L. Chapman ◽  
Blair D. Johnson ◽  
James R. Sackett ◽  
Mark D. Parker ◽  
Zachary J. Schlader

The purpose of this study was to test the hypothesis that consuming a soft drink (i.e., a high-fructose, caffeinated beverage) during and following exercise in the heat elevates biomarkers of acute kidney injury (AKI) in humans. Twelve healthy adults drank 2 liters of an assigned beverage during 4 h of exercise in the heat [35.1 (0.1)°C, 61 (5)% relative humidity] in counterbalanced soft drink and water trials, and ≥1 liter of the same beverage after leaving the laboratory. Stage 1 AKI (i.e., increased serum creatinine ≥0.30 mg/dl) was detected at postexercise in 75% of participants in the Soft Drink trial compared with 8% in Water trial ( P = 0.02). Furthermore, urinary neutrophil gelatinase-associated lipocalin (NGAL), a biomarker of AKI, was higher during an overnight collection period after the Soft Drink trial compared with Water in both absolute concentration [6 (4) ng/dl vs. 5 (4) ng/dl, P < 0.04] and after correcting for urine flow rate [6 (7) (ng/dl)/(ml/min) vs. 4 (4) (ng/dl)/(ml/min), P = 0.03]. Changes in serum uric acid from preexercise were greater in the Soft Drink trial than the Water trial at postexercise ( P < 0.01) and 24 h ( P = 0.05). There were greater increases from preexercise in serum copeptin, a stable marker of vasopressin, at postexercise in the Soft Drink trial ( P < 0.02) than the Water trial. These findings indicate that consuming a soft drink during and following exercise in the heat induces AKI, likely via vasopressin-mediated mechanisms.


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