High Throughput Screening to Modify Surface Properties and Obtain High Performance Membranes

2009 ◽  
pp. 209-218
Author(s):  
Mingyan Zhou ◽  
James E. Kilduff ◽  
Georges Belfort
Keyword(s):  
Surface Properties ◽  
High Throughput ◽  
High Throughput Screening ◽  
High Performance ◽  
Modify Surface

scholarly journals A Micromethod for Polyphenol High-Throughput Screening Saves 90 Percent Reagents and Sample Volume

Antioxidants ◽  
2019 ◽  
Vol 9 (1) ◽  
pp. 11
Author(s):  
Franz Tatzber ◽  
Willibald Wonisch ◽  
Sonja Lackner ◽  
Meinrad Lindschinger ◽  
Werner Pursch ◽  
...  
Keyword(s):  
High Throughput ◽  
High Throughput Screening ◽  
High Performance ◽  
Reliable Method ◽  
Animal Health ◽  
Sample Volume ◽  
Total Polyphenol ◽  
Ample Evidence ◽  
Natural Substances ◽  
Layer Chromatography

There is ample evidence that polyphenols are important natural substances with pronounced antioxidative properties. This study aimed to develop a fast and reliable method to determine total polyphenol content (TPC) in foodstuffs and human samples. The microtitration format offers the advantage of low sample volumes in the microlitre range, facilitating high-throughput screening with 40 samples simultaneously. We accordingly adjusted the so-called Folin–Ciocalteu method to a microtitre format (polyphenols microtitre—PPm) with 90% reduction of reagents. The assay was standardized with gallic acid in the range between 0.1 and 3 mM, using a 20 µL sample volume. The intra-assay coefficient of variation (CV) was less than 5%, and inter-assay CV was in the range of 10%. Wavelength was measured at 766 nm after two hours of incubation. This micromethod correlates significantly with both the classical Folin–Ciocalteu method and High-Performance Thin-Layer Chromatography (HPTLC) (r2 = 0.9829). We further observed a significant correlation between PPm and total antioxidants (r2 = 0.918). The highest polyphenol concentrations were obtained for red, blue, and black fruits, vegetables, and juices. Extracts of red grapes could be harvested almost sugar free and might serve as a basis for polyphenol supplementation. Beer, flour, and bread contained polyphenol concentrations sufficient to meet the minimal daily requirement. We conclude that PPm is a sensitive and reliable method that detects polyphenols even in samples diluted 10-fold. The literature strongly recommends further investigations on the effects of polyphenol uptake on human and animal health.

Discovery of High Performance Thermoelectric Chalcogenides Through First-Principles High-Throughput Screening

2021 ◽  
Author(s):  
Tao Fan ◽  
Artem R. Oganov
Keyword(s):  
Transport Properties ◽  
High Throughput ◽  
Thermoelectric Materials ◽  
First Principles ◽  
High Throughput Screening ◽  
High Performance ◽  
Energy Conversion Efficiency ◽  
High Energy ◽  
Energy And Environment ◽  
High Energy Conversion Efficiency

Searching for thermoelectric materials with high energy conversion efficiency is important to solve the energy and environment issues of our society. In this work, we studied the thermoelectric-related transport properties...

scholarly journals Development of a Rapid Fluorescence-Based High-Throughput Screening Assay to Identify Novel Kynurenine 3-Monooxygenase Inhibitor Scaffolds

2018 ◽  
Vol 23 (6) ◽  
pp. 554-560 ◽  
Author(s):  
K. R. Jacobs ◽  
G. J. Guillemin ◽  
D. B. Lovejoy
Keyword(s):  
High Performance Liquid Chromatography ◽  
High Throughput ◽  
High Throughput Screening ◽  
High Performance ◽  
Excellent Performance ◽  
Statistical Parameters ◽  
Screening Assay ◽  
High Throughput Screening Assay ◽  
Z Value ◽  
Inhibitor Scaffolds

Kynurenine 3-monooxygenase (KMO) is a well-validated therapeutic target for the treatment of neurodegenerative diseases, including Alzheimer’s disease (AD) and Huntington’s disease (HD). This work reports a facile fluorescence-based KMO assay optimized for high-throughput screening (HTS) that achieves a throughput approximately 20-fold higher than the fastest KMO assay currently reported. The screen was run with excellent performance (average Z′ value of 0.80) from 110,000 compounds across 341 plates and exceeded all statistical parameters used to describe a robust HTS assay. A subset of molecules was selected for validation by ultra-high-performance liquid chromatography, resulting in the confirmation of a novel hit with an IC50 comparable to that of the well-described KMO inhibitor Ro-61-8048. A medicinal chemistry program is currently underway to further develop our novel KMO inhibitor scaffolds.

scholarly journals Development of a Novel Ectonucleotidase Assay Suitable for High-Throughput Screening

2012 ◽  
Vol 17 (7) ◽  
pp. 993-998 ◽  
Author(s):  
Kris F. Sachsenmeier ◽  
Carl Hay ◽  
Erin Brand ◽  
Lori Clarke ◽  
Kim Rosenthal ◽  
...  
Keyword(s):  
Malachite Green ◽  
High Throughput ◽  
High Throughput Screening ◽  
High Performance ◽  
Adenosine Monophosphate ◽  
Screening Assay ◽  
Highly Sensitive ◽  
Development And Validation ◽  
Multiple Samples

5′-Ectonucleotidase (NT5E) catalyzes the conversion of adenosine monophosphate to adenosine and free phosphate. The role of this ectonucleotidase and its production of adenosine are linked with immune function, angiogenesis, and cancer. NT5E activity is typically assayed either by chromatographic quantification of substrates and products using high-performance liquid chromatography (HPLC) or by quantification of free phosphate using malachite green. These methods are not suitable for robust screening assays of NT5E activity. HPLC is not readily suitable for the rapid and efficient assay of multiple samples and malachite green is highly sensitive to the phosphate-containing buffers common in various media and sample buffers. Here the development and validation of a novel high-throughput ectonucleotidase screening assay are described, which makes use of a luciferase-based assay reagent, the Promega CellTiter-Glo kit, to measure the catabolism of AMP by NT5E. This multiwell plate-based assay facilitates the screening of potential ectonucleotidase antagonists and is unaffected by the presence of contaminating phosphate molecules present in screening samples.

scholarly journals Current High-Throughput Approaches of Screening Modulatory Effects of Xenobiotics on Cytochrome P450 (CYP) Enzymes

2018 ◽  
Vol 7 (4) ◽  
pp. 29 ◽  
Author(s):  
Yee Ung ◽  
Chin Ong ◽  
Yan Pan
Keyword(s):  
Cytochrome P450 ◽  
High Throughput ◽  
Enzyme Activities ◽  
High Throughput Screening ◽  
High Performance ◽  
Screening Methods ◽  
Drug Metabolizing Enzyme ◽  
Enzyme Superfamily ◽  
Wide Range ◽  
Metabolizing Enzyme

Cytochrome P450 (CYP) is a critical drug-metabolizing enzyme superfamily. Modulation of CYP enzyme activities has the potential to cause drug–drug/herb interactions. Drug–drug/herb interactions can lead to serious adverse drug reactions (ADRs) or drug failures. Therefore, there is a need to examine the modulatory effects of new drug entities or herbal preparations on a wide range of CYP isoforms. The classic method of quantifying CYP enzyme activities is based on high-performance liquid chromatography (HPLC), which is time- and reagent-consuming. In the past two decades, high-throughput screening methods including fluorescence-based, luminescence-based, and mass-spectrometry-based assays have been developed and widely applied to estimate CYP enzyme activities. In general, these methods are faster and use lower volume of reagents than HPLC. However, each high-throughput method has its own limitations. Investigators may make a selection of these methods based on the available equipment in the laboratory, budget, and enzyme sources supplied. Furthermore, the current high-throughput systems should look into developing a reliable automation mechanism to accomplish ultra-high-throughput screening in the near future.

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