scholarly journals Analysis of a Novel Insect Cell Culture Medium-Based Growth Medium for Bartonella Species

2008 ◽  
Vol 74 (16) ◽  
pp. 5224-5227 ◽  
Author(s):  
Tanja Riess ◽  
Florian Dietrich ◽  
Katja V. Schmidt ◽  
Patrick O. Kaiser ◽  
Heinz Schwarz ◽  
...  
Keyword(s):  
Cell Culture ◽  
Insect Cell ◽  
Culture Medium ◽  
Expression Patterns ◽  
Insect Cell Culture ◽  
Host Cells ◽  
Cell Culture Medium ◽  
Bacterial Protein ◽  
Bartonella Species ◽  
Slow Growing

ABSTRACT Human- and animal-pathogenic Bartonella species are fastidious and slow-growing bacteria difficult to isolate and cultivate. We describe a novel, easy-to-prepare liquid medium for the fast and reliable growth of several Bartonella spp. that does not affect bacterial protein expression patterns or interactions with host cells.

Gas Bubbles and Their Influence on Microorganisms

1998 ◽  
Vol 51 (1) ◽  
pp. 113-120 ◽  
Author(s):  
Jeffrey J. Chalmers
Keyword(s):  
Cell Culture ◽  
Insect Cell ◽  
Culture Medium ◽  
Cell Damage ◽  
Gas Bubbles ◽  
Insect Cell Culture ◽  
Review Article ◽  
Cell Culture Medium

A review of the interactions of microorganisms and bubbles is presented. Particular emphasis is given to the damage that results when gas bubbles are introduced into animal and insect cell culture medium. The mechanism of this damage is presented as well as a discussion of the protective additives that have been found to reduce cell damage from gas bubbles. This review article has 43 references.

Insect Cell Culture Medium Selection and Optimisation Based on Monitoring and Economical Considerations

2005 ◽  
pp. 103-106
Author(s):  
M. Lecina ◽  
A. Soley ◽  
P. Passamani ◽  
A. Casablancas ◽  
J. De Gràcia ◽  
...  
Keyword(s):  
Cell Culture ◽  
Insect Cell ◽  
Culture Medium ◽  
Insect Cell Culture ◽  
Cell Culture Medium ◽  
Medium Selection

Adaptation of an insect cell line (Agallia constricta) in a mammalian cell culture medium

In Vitro ◽  
1973 ◽  
Vol 8 (5) ◽  
pp. 375-378 ◽  
Author(s):  
Arthur H. Intosh ◽  
K. Maramorosch ◽  
C. Rechtoris
Keyword(s):  
Cell Culture ◽  
Cell Line ◽  
Mammalian Cell ◽  
Insect Cell ◽  
Culture Medium ◽  
Mammalian Cell Culture ◽  
Insect Cell Line ◽  
Cell Culture Medium

scholarly journals Long-Term Culture of Giardia lamblia in Cell Culture Medium Requires Intimate Association with Viable Mammalian Cells

2019 ◽  
Vol 87 (11) ◽  
Author(s):  
Theodore E. Nash
Keyword(s):  
Cell Culture ◽  
Cell Line ◽  
Cell Lines ◽  
Giardia Lamblia ◽  
Mammalian Cells ◽  
Culture Medium ◽  
Host Cells ◽  
Cell Culture Medium ◽  
Heterologous Cell

ABSTRACT Giardia lamblia is usually cultured axenically in TYI-S-33, a complex medium which does not permit survival and growth of mammalian cells. Likewise, medium commonly used to maintain and grow mammalian cells does not support healthy trophozoite survival for more than a few hours. The inability to coculture trophozoites and epithelial cells under optimal conditions limits studies of their interactions as well as interpretation of results. Trophozoites of the WB isolate but not the GS isolate were repeatedly adapted to grow stably in long-term cocultures with Caco2, Cos7, and mouse tumor rectal (RIT) cell lines using hybridoma-screened Dulbecco’s modified Eagle’s medium and 10% fetal calf serum. Giardia did not grow in spent cell culture medium or when separated by a permeable membrane using transwell methodology. Giardia chronically cocultured with specific cell lines became adapted (conditioned). These Giardia cocultures grew better than nonconditioned trophozoites, and the cell lines differed in their ability to support trophozoite growth in the order of RIT > Cos7 > Caco2. Trophozoites conditioned on one cell line and then grown in the presence of a heterologous cell line changed their growth rate to that seen in conditioned Giardia from the heterologous cell line. Trophozoite survival required intimate contact with cells, suggesting that trophozoites obtain an essential nutrient or growth factor from mammalian cells. This may explain why Giardia trophozoites adhere to the small intestinal epithelium during human and animal infections. This coculture system will be useful to understand the complex interactions between the host cells and parasite.

3-iodothyronamine (T1AM) is taken up and rapidly metabolized in cell culture medium only at low concentration

2020 ◽  
Author(s):  
Federica Saponaro ◽  
Marco Borsò ◽  
Sara Verlotta ◽  
Lavinia Bandini ◽  
Alessandro Saba ◽  
...  
Keyword(s):  
Cell Culture ◽  
Culture Medium ◽  
Cell Culture Medium ◽  
Low Concentration

Effect of Atmospheric-Pressure Helium Plasma Jet on Cell Culture Medium

2013 ◽  
Vol 133 (5) ◽  
pp. 278-285
Author(s):  
Norimitsu Takamura ◽  
Douyan Wang ◽  
Takao Satoh ◽  
Takao Namihira ◽  
Hisato Saitoh ◽  
...  
Keyword(s):  
Cell Culture ◽  
Atmospheric Pressure ◽  
Culture Medium ◽  
Plasma Jet ◽  
Helium Plasma ◽  
Cell Culture Medium
Sign in / Sign up

Export Citation Format

Share Document