Gas Bubbles and Their Influence on Microorganisms

1998 ◽  
Vol 51 (1) ◽  
pp. 113-120 ◽  
Author(s):  
Jeffrey J. Chalmers
Keyword(s):  
Cell Culture ◽  
Insect Cell ◽  
Culture Medium ◽  
Cell Damage ◽  
Gas Bubbles ◽  
Insect Cell Culture ◽  
Review Article ◽  
Cell Culture Medium

A review of the interactions of microorganisms and bubbles is presented. Particular emphasis is given to the damage that results when gas bubbles are introduced into animal and insect cell culture medium. The mechanism of this damage is presented as well as a discussion of the protective additives that have been found to reduce cell damage from gas bubbles. This review article has 43 references.

scholarly journals Analysis of a Novel Insect Cell Culture Medium-Based Growth Medium for Bartonella Species

2008 ◽  
Vol 74 (16) ◽  
pp. 5224-5227 ◽  
Author(s):  
Tanja Riess ◽  
Florian Dietrich ◽  
Katja V. Schmidt ◽  
Patrick O. Kaiser ◽  
Heinz Schwarz ◽  
...  
Keyword(s):  
Cell Culture ◽  
Insect Cell ◽  
Culture Medium ◽  
Expression Patterns ◽  
Insect Cell Culture ◽  
Host Cells ◽  
Cell Culture Medium ◽  
Bacterial Protein ◽  
Bartonella Species ◽  
Slow Growing

ABSTRACT Human- and animal-pathogenic Bartonella species are fastidious and slow-growing bacteria difficult to isolate and cultivate. We describe a novel, easy-to-prepare liquid medium for the fast and reliable growth of several Bartonella spp. that does not affect bacterial protein expression patterns or interactions with host cells.

Insect Cell Culture Medium Selection and Optimisation Based on Monitoring and Economical Considerations

2005 ◽  
pp. 103-106
Author(s):  
M. Lecina ◽  
A. Soley ◽  
P. Passamani ◽  
A. Casablancas ◽  
J. De Gràcia ◽  
...  
Keyword(s):  
Cell Culture ◽  
Insect Cell ◽  
Culture Medium ◽  
Insect Cell Culture ◽  
Cell Culture Medium ◽  
Medium Selection

Adaptation of an insect cell line (Agallia constricta) in a mammalian cell culture medium

In Vitro ◽  
1973 ◽  
Vol 8 (5) ◽  
pp. 375-378 ◽  
Author(s):  
Arthur H. Intosh ◽  
K. Maramorosch ◽  
C. Rechtoris
Keyword(s):  
Cell Culture ◽  
Cell Line ◽  
Mammalian Cell ◽  
Insect Cell ◽  
Culture Medium ◽  
Mammalian Cell Culture ◽  
Insect Cell Line ◽  
Cell Culture Medium

scholarly journals Bicarbonate Plays a Critical Role in the Generation of Cytotoxicity during SIN-1 Decomposition in Culture Medium

2012 ◽  
Vol 2012 ◽  
pp. 1-15 ◽  
Author(s):  
Kyo Shirai ◽  
Tatsumi Okada ◽  
Kanako Konishi ◽  
Hiroshi Murata ◽  
Soichiro Akashi ◽  
...  
Keyword(s):  
Cell Culture ◽  
Culture Medium ◽  
Nitrosative Stress ◽  
Cell Damage ◽  
Critical Role ◽  
Cell Culture Medium ◽  
Cytotoxic Effects ◽  
Related Cell ◽  
Cytotoxic Substance ◽  
Over Time

3-Morpholinosydnonimine (SIN-1) is used as a donor of peroxynitrite (ONOO−) in various studies. We demonstrated, however, that, the cell-culture medium remains cytotoxic to PC12 cells even after almost complete SIN-1 decomposition, suggesting that reaction product(s) in the medium, rather thanONOO−, exert cytotoxic effects. Here, we clarified that significant cytotoxicity persists after SIN-1 decomposes in bicarbonate, a component of the culture medium, but not in NaOH. Cytotoxic SIN-1-decomposed bicarbonate, which lacks both oxidizing and nitrosating activities, degrades to innocuous state over time. The extent of SIN-1 cytotoxicity, irrespective of its fresh or decomposed state, appears to depend on the total number of initial SIN-1 molecules per cell, rather than its concentration, and involves oxidative/nitrosative stress-related cell damage. These results suggest that, despite its low abundance, the bicarbonate-dependent cytotoxic substance that accumulates in the medium during SIN-1 breakdown is the cytotoxic entity of SIN-1.

3-iodothyronamine (T1AM) is taken up and rapidly metabolized in cell culture medium only at low concentration

2020 ◽  
Author(s):  
Federica Saponaro ◽  
Marco Borsò ◽  
Sara Verlotta ◽  
Lavinia Bandini ◽  
Alessandro Saba ◽  
...  
Keyword(s):  
Cell Culture ◽  
Culture Medium ◽  
Cell Culture Medium ◽  
Low Concentration

Effect of Atmospheric-Pressure Helium Plasma Jet on Cell Culture Medium

2013 ◽  
Vol 133 (5) ◽  
pp. 278-285
Author(s):  
Norimitsu Takamura ◽  
Douyan Wang ◽  
Takao Satoh ◽  
Takao Namihira ◽  
Hisato Saitoh ◽  
...  
Keyword(s):  
Cell Culture ◽  
Atmospheric Pressure ◽  
Culture Medium ◽  
Plasma Jet ◽  
Helium Plasma ◽  
Cell Culture Medium
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