Ultrasmall Magnetically Engineered Ag2Se Quantum Dots for Instant Efficient Labeling and Whole-Body High-Resolution Multimodal Real-Time Tracking of Cell-Derived Microvesicles

2016 ◽  
Vol 138 (6) ◽  
pp. 1893-1903 ◽  
Author(s):  
Jing-Ya Zhao ◽  
Gang Chen ◽  
Yi-Ping Gu ◽  
Ran Cui ◽  
Zhi-Ling Zhang ◽  
...  
Materials ◽  
2019 ◽  
Vol 12 (13) ◽  
pp. 2191 ◽  
Author(s):  
Yu Wang ◽  
Nan Xu ◽  
Yongkai He ◽  
Jingyun Wang ◽  
Dan Wang ◽  
...  

Fluorescence imaging offers a new approach to visualize real-time details on a cellular level in vitro and in vivo without radioactive damage. Poor light stability of organic fluorescent dyes makes long-term imaging difficult. Due to their outstanding optical properties and unique structural features, graphene quantum dots (GQDs) are promising in the field of imaging for real-time tracking in vivo. At present, GQDs are mainly loaded on the surface of nanoparticles. In this study, we developed an efficient and convenient one-pot method to load GQDs into nanoparticles, leading to longer metabolic processes in blood and increased delivery of GQDs to tumors. Optical-magneto ferroferric oxide@polypyrrole (Fe3O4@PPy) core-shell nanoparticles were chosen for their potential use in cancer therapy. The in vivo results demonstrated that by loading GQDs, it was possible to monitor the distribution and metabolism of nanoparticles. This study provided new insights into the application of GQDs in long-term in vivo real-time tracking.


2007 ◽  
Vol 67 (3) ◽  
pp. 1138-1144 ◽  
Author(s):  
Hiroshi Tada ◽  
Hideo Higuchi ◽  
Tomonobu M. Wanatabe ◽  
Noriaki Ohuchi

2021 ◽  
Author(s):  
Wysterlânya Kyury Pereira Barros ◽  
Marcelo Fernandes

This work proposes an implementation in Field Programmable GateArray (FPGA) of the Otsu’s method applied to real-time trackingof worms called Caenorhabditis elegans. Real-time tracking is necessaryto measure changes in the worm’s behavior in response totreatment with Ribonucleic Acid (RNA) interference. Otsu’s methodis a global thresholding algorithm used to define an optimal thresholdbetween two classes. However, this technique in real-time applicationsassociated with the processing of high-resolution videoshas a high computational cost because of the massive amount ofdata generated. Otsu’s algorithm needs to identify the worms ineach frame captured by a high-resolution camera in a real-timeanalysis of the worm’s behavior. Thus, this work proposes a highperformanceimplementation of Otsu’s algorithm in FPGA. Theresults show it was possible to achieve a speedup up to 5 timeshigher than similar works in the literature.


RSC Advances ◽  
2017 ◽  
Vol 7 (85) ◽  
pp. 53822-53829 ◽  
Author(s):  
Ashwin Kumar Narasimhan ◽  
Swathi Lakshmi B ◽  
Tuhin Subhra Santra ◽  
M. S. Ramachandra Rao ◽  
Ganapathy Krishnamurthi

Synthesis of graphene quantom dots for single live cell imaging andin vivofluorescence imaging.


Author(s):  
Kenneth Krieg ◽  
Richard Qi ◽  
Douglas Thomson ◽  
Greg Bridges

Abstract A contact probing system for surface imaging and real-time signal measurement of deep sub-micron integrated circuits is discussed. The probe fits on a standard probe-station and utilizes a conductive atomic force microscope tip to rapidly measure the surface topography and acquire real-time highfrequency signals from features as small as 0.18 micron. The micromachined probe structure minimizes parasitic coupling and the probe achieves a bandwidth greater than 3 GHz, with a capacitive loading of less than 120 fF. High-resolution images of submicron structures and waveforms acquired from high-speed devices are presented.


2006 ◽  
Author(s):  
Tian He ◽  
Lin Gu ◽  
Liqian Luo ◽  
Ting Yan ◽  
John A. Stankovic ◽  
...  

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