scholarly journals Oxygenated graphene quantum dots (GQDs) synthesized using laser ablation for long-term real-time tracking and imaging

RSC Advances ◽  
2017 ◽  
Vol 7 (85) ◽  
pp. 53822-53829 ◽  
Author(s):  
Ashwin Kumar Narasimhan ◽  
Swathi Lakshmi B ◽  
Tuhin Subhra Santra ◽  
M. S. Ramachandra Rao ◽  
Ganapathy Krishnamurthi
Keyword(s):  
Quantum Dots ◽  
Laser Ablation ◽  
Real Time ◽  
Fluorescence Imaging ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Graphene Quantum Dots ◽  
Real Time Tracking

Synthesis of graphene quantom dots for single live cell imaging andin vivofluorescence imaging.

scholarly journals Loading Graphene Quantum Dots into Optical-Magneto Nanoparticles for Real-Time Tracking In Vivo

Materials ◽  
2019 ◽  
Vol 12 (13) ◽  
pp. 2191 ◽  
Author(s):  
Yu Wang ◽  
Nan Xu ◽  
Yongkai He ◽  
Jingyun Wang ◽  
Dan Wang ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Real Time ◽  
Fluorescent Dyes ◽  
Graphene Quantum Dots ◽  
Shell Nanoparticles ◽  
One Pot ◽  
Real Time Tracking

Fluorescence imaging offers a new approach to visualize real-time details on a cellular level in vitro and in vivo without radioactive damage. Poor light stability of organic fluorescent dyes makes long-term imaging difficult. Due to their outstanding optical properties and unique structural features, graphene quantum dots (GQDs) are promising in the field of imaging for real-time tracking in vivo. At present, GQDs are mainly loaded on the surface of nanoparticles. In this study, we developed an efficient and convenient one-pot method to load GQDs into nanoparticles, leading to longer metabolic processes in blood and increased delivery of GQDs to tumors. Optical-magneto ferroferric oxide@polypyrrole (Fe3O4@PPy) core-shell nanoparticles were chosen for their potential use in cancer therapy. The in vivo results demonstrated that by loading GQDs, it was possible to monitor the distribution and metabolism of nanoparticles. This study provided new insights into the application of GQDs in long-term in vivo real-time tracking.

In vivo Real-time Tracking of Single Quantum Dots Conjugated with Monoclonal Anti-HER2 Antibody in Tumors of Mice

2007 ◽  
Vol 67 (3) ◽  
pp. 1138-1144 ◽  
Author(s):  
Hiroshi Tada ◽  
Hideo Higuchi ◽  
Tomonobu M. Wanatabe ◽  
Noriaki Ohuchi
Keyword(s):  
Quantum Dots ◽  
Real Time ◽  
Single Quantum ◽  
Single Quantum Dots ◽  
Real Time Tracking

Imaging Takes a Quantum Leap

Physiology ◽  
2004 ◽  
Vol 19 (6) ◽  
pp. 322-325 ◽  
Author(s):  
Diane S. Lidke ◽  
Donna J. Arndt-Jovin
Keyword(s):  
Quantum Dots ◽  
Surface Chemistry ◽  
Fluorescence Imaging ◽  
Emission Spectra ◽  
Quantum Leap ◽  
In Vivo Fluorescence Imaging ◽  
Biomolecular Conjugation ◽  
Narrow Emission

Semiconducting nanocrystals, or quantum dots (QDs), have emerged as a new tool in physiological imaging, combining high brilliance, photostability, broad excitation but very narrow emission spectra, and surface chemistry compatible with biomolecular conjugation. In this review, we demonstrate the power of QDs in diverse applications, including long-term in vivo fluorescence imaging.

scholarly journals The Role of Amino Acids in Neurotransmission and Fluorescent Tools for Their Detection

2020 ◽  
Vol 21 (17) ◽  
pp. 6197
Author(s):  
Rochelin Dalangin ◽  
Anna Kim ◽  
Robert E. Campbell
Keyword(s):  
Amino Acids ◽  
Quantum Dots ◽  
Amino Acid ◽  
Controlled Release ◽  
Fluorescence Imaging ◽  
Small Molecule ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Synthetic Dyes

Neurotransmission between neurons, which can occur over the span of a few milliseconds, relies on the controlled release of small molecule neurotransmitters, many of which are amino acids. Fluorescence imaging provides the necessary speed to follow these events and has emerged as a powerful technique for investigating neurotransmission. In this review, we highlight some of the roles of the 20 canonical amino acids, GABA and β-alanine in neurotransmission. We also discuss available fluorescence-based probes for amino acids that have been shown to be compatible for live cell imaging, namely those based on synthetic dyes, nanostructures (quantum dots and nanotubes), and genetically encoded components. We aim to provide tool developers with information that may guide future engineering efforts and tool users with information regarding existing indicators to facilitate studies of amino acid dynamics.

scholarly journals Live‐Cell Imaging: Time‐Gated FRET Nanoprobes for Autofluorescence‐Free Long‐Term In Vivo Imaging of Developing Zebrafish (Adv. Mater. 39/2020)

2020 ◽  
Vol 32 (39) ◽  
pp. 2070291 ◽  
Author(s):  
Marcelina Cardoso Dos Santos ◽  
Ingrid Colin ◽  
Gabriel Ribeiro Dos Santos ◽  
Kimihiro Susumu ◽  
Michaël Demarque ◽  
...  
Keyword(s):  
In Vivo Imaging ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell ◽  
Imaging Time

Preparation of highly fluorescent sulfur doped graphene quantum dots for live cell imaging

2018 ◽  
Vol 197 ◽  
pp. 147-152 ◽  
Author(s):  
Kaixiang Jin ◽  
Hui Gao ◽  
Luhao Lai ◽  
Yuqian Pang ◽  
Shiyuan Zheng ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Graphene Quantum Dots ◽  
Live Cell ◽  
Doped Graphene

New Approaches to Analyse Axon-Oligodendrocyte Communication in vivo

e-Neuroforum ◽  
2017 ◽  
Vol 23 (4) ◽  
Author(s):  
Tim Czopka ◽  
Franziska Auer
Keyword(s):  
Signal Transduction ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Genetic Manipulation ◽  
Model Organism ◽  
Myelinated Axon ◽  
Research Area ◽  
Open Questions

AbstractA major challenge for understanding our nervous system is to elucidate how its constituting cells coordinate each other to form and maintain a functional organ. The interaction between neurons and oligodendrocytes represents a unique cellular entity. Oligodendrocytes myelinate axons by tightly ensheathing them. Myelination regulates speed of signal transduction, thus communication between neurons, and supports long-term axonal health. Despite their importance, we still have large gaps in our understanding of the mechanisms underlying myelinated axon formation, remodelling and repair. Zebrafish represent an increasingly popular model organism, particularly due to their suitability for live cell imaging and genetic manipulation. Here, we provide an overview about this research area, describe how zebrafish have helped understanding mechanisms of myelination, and discuss how zebrafish may help addressing open questions related to the control of axon-oligodendrocyte interactions.

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