Characterization of storage proteins extracted from Avena sativa seed protein bodies

1982 ◽  
Vol 30 (1) ◽  
pp. 32-36 ◽  
Author(s):  
Jean Claude Pernollet ◽  
Su Il Kim ◽  
Jacques Mosse
Keyword(s):  
Avena Sativa ◽  
Seed Protein ◽  
Storage Proteins ◽  
Protein Bodies

scholarly journals Purification and initial characterization of ubiquitin from the higher plant, Avena sativa.

1985 ◽  
Vol 260 (22) ◽  
pp. 12015-12021 ◽  
Author(s):  
R D Vierstra ◽  
S M Langan ◽  
A L Haas
Keyword(s):  
Avena Sativa ◽  
Higher Plant ◽  
Initial Characterization

scholarly journals A Single Seed Protein Extraction Protocol for Characterizing Brassica Seed Storage Proteins

Agronomy ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 107
Author(s):  
Mahmudur Rahman ◽  
Lei Liu ◽  
Bronwyn J. Barkla
Keyword(s):  
Seed Protein ◽  
Storage Proteins ◽  
Protein Extraction ◽  
Seed Storage ◽  
Seed Storage Proteins ◽  
Protein Yield ◽  
Tissue Type ◽  
Single Seed ◽  
Material Optimization ◽  
Hazardous Chemical

Rapeseed oil-extracted expeller cake mostly contains protein. Various approaches have been used to isolate, detect and measure proteins in rapeseeds, with a particular focus on seed storage proteins (SSPs). To maximize the protein yield and minimize hazardous chemical use, isolation costs and the loss of seed material, optimization of the extraction method is pivotal. For some studies, it is also necessary to minimize or avoid seed-to-seed cross-contamination for phenotyping and single-tissue type analysis to know the exact amount of any bioactive component in a single seed, rather than a mixture of multiple seeds. However, a simple and robust method for single rapeseed seed protein extraction (SRPE) is unavailable. To establish a strategy for optimizing SRPE for downstream gel-based protein analysis, yielding the highest amount of SSPs in the most economical and rapid way, a variety of different approaches were tested, including variations to the seed pulverization steps, changes to the compositions of solvents and reagents and adjustments to the protein recovery steps. Following SRPE, 1D-SDS-PAGE was used to assess the quality and amount of proteins extracted. A standardized SRPE procedure was developed and then tested for yield and reproducibility. The highest protein yield and quality were obtained using a ball grinder with stainless steel beads in Safe-Lock microcentrifuge tubes with methanol as the solvent, providing a highly efficient, economic and effective method. The usefulness of this SRPE was validated by applying the procedure to extract protein from different Brassica oilseeds and for screening an ethyl methane sulfonate (EMS) mutant population of Brassica rapa R-0-18. The outcomes provide useful methodology for identifying and characterizing the SSPs in the SRPE.

scholarly journals Characterization of Storage Proteins in Wild (Glycine soja) and Cultivated (Glycine max) Soybean Seeds Using Proteomic Analysis

2006 ◽  
Vol 54 (8) ◽  
pp. 3114-3120 ◽  
Author(s):  
Savithiry S. Natarajan ◽  
Chenping Xu ◽  
Hanhong Bae ◽  
Thomas J. Caperna ◽  
Wesley M. Garrett
Keyword(s):  
Glycine Max ◽  
Proteomic Analysis ◽  
Storage Proteins ◽  
Glycine Soja ◽  
Soybean Seeds

Characterization of two integral membrane proteins located in the protein bodies of pumpkin seeds

1995 ◽  
Vol 28 (6) ◽  
pp. 1089-1101 ◽  
Author(s):  
Kaori Inoue ◽  
Yuka Takeuchi ◽  
Mikio Nishimura ◽  
Ikuko Hara-Nishimura
Keyword(s):  
Membrane Proteins ◽  
Integral Membrane Proteins ◽  
Protein Bodies ◽  
Pumpkin Seeds

Synthesization and characterization of lotus seed protein‐based curcumin microcapsules with enhanced solubility, stability, and sustained release

2021 ◽  
Author(s):  
Ya Su ◽  
Ying Chen ◽  
Li Zhang ◽  
Benu Adhikari ◽  
Baoguo Xu ◽  
...  
Keyword(s):  
Sustained Release ◽  
Seed Protein ◽  
Lotus Seed ◽  
Enhanced Solubility

scholarly journals Characterization of Radish (Raphanus sativus) Storage Proteins

1984 ◽  
Vol 74 (3) ◽  
pp. 487-493 ◽  
Author(s):  
Monique Laroche ◽  
Lorette Aspart ◽  
Michel Delseny ◽  
Paul Penon
Keyword(s):  
Raphanus Sativus ◽  
Storage Proteins

scholarly journals Comparative characterization of starch biopolymers extracted from cereals using two different techniques

2020 ◽  
pp. 21-26
Author(s):  
Marisa Rivera-Arredondo ◽  
Mario Alberto Rodríguez-Ángeles ◽  
Verónica de Jesús Morales-Félix ◽  
Marina Gaytán-Ruelas
Keyword(s):  
Oryza Sativa ◽  
Avena Sativa ◽  
Chemical Characteristics ◽  
Crystalline Region ◽  
Environmental Legislation ◽  
Ecological Awareness ◽  
Comparative Characterization ◽  
Naoh Treatment

In the field of research and consequent elaboration of biopolymers it has been growing in the last decade, either due to the hardening of the environmental legislation of each country or due to ecological awareness, in any case the term biopolymer is quite broad, these being used as material premium in medicines, food supplements and of course in the production of bioplastics. Since the topic of interest is the comparative characterization of biopolymers using starch extracted from two different cereals, oats and rice, using the alkaline technique with NaOH treatment and the milling technique, assuming that the starch extracted from the Oryza sativa cereal presents chemical characteristics, geometric and mechanical superior in both techniques used unlike the polymers made with Avena sativa starch. These results suggest that the starch quality of each cereal is relevant for the production of biopolymers [1]. The commercially significant properties of starch, such as its mechanical strength and flexibility, depend on the strength and character of the crystalline region, which depends on the ratio of amylose and amylopectin.

Sign in / Sign up

Export Citation Format

Share Document