Structure−Function Correlation of Chloroquine and Analogues as Transgene Expression Enhancers in Nonviral Gene Delivery

2006 ◽  
Vol 49 (22) ◽  
pp. 6522-6531 ◽  
Author(s):  
Jianjun Cheng ◽  
Ryan Zeidan ◽  
Swaroop Mishra ◽  
Aijie Liu ◽  
Suzie H. Pun ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Structure Function ◽  
Transgene Expression ◽  
Nonviral Gene Delivery ◽  
Function Correlation

Reduction-Sensitive Lipopolyamines as a Novel Nonviral Gene Delivery System for Modulated Release of DNA with Improved Transgene Expression

2000 ◽  
Vol 43 (23) ◽  
pp. 4377-4387 ◽  
Author(s):  
Gerardo Byk ◽  
Barbara Wetzer ◽  
Marc Frederic ◽  
Catherine Dubertret ◽  
Bruno Pitard ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Delivery System ◽  
Transgene Expression ◽  
Nonviral Gene Delivery ◽  
Gene Delivery System

Comparative Investigation of Polymeric and Nanoparticle Vehicles for Transgene Delivery

Nano LIFE ◽  
2016 ◽  
Vol 06 (02) ◽  
pp. 1641001 ◽  
Author(s):  
James Ramos ◽  
Kaushal Rege
Keyword(s):  
Gene Delivery ◽  
Transgene Expression ◽  
Comparative Investigation ◽  
Nonviral Gene Delivery ◽  
Luciferase Expression ◽  
Hydrodynamic Diameter ◽  
Nucleic Acid Delivery ◽  
Polymeric Systems ◽  
Transgene Delivery

Effective design of nanoparticle systems can have a significant impact on therapeutic delivery. Physicochemical factors including size, shape and surface chemistry of nanoparticles can play a significant role in determining the efficacy of drug and gene delivery to cells. Polymeric as well as inorganic nanoparticle systems have been investigated as vehicles for nonviral gene delivery of transgenes. However, a head-to-head comparison of these different systems is largely lacking. In this study, we compare three related delivery systems, polymer, polymer-templated gold nanospheres and polymer-coated gold nanorods, for their respective in vitro transgene expression efficacies. Significant differences were seen in the hydrodynamic diameter and zeta potential for each of these different vehicles. Nevertheless, transgene (luciferase) expression efficacies and cytotoxicities were found to be similar for these three vehicles under different conditions. Our results indicate that polymeric systems can be used for synthesis or modification of nanoparticle systems with negligible variations in their transgene expression efficacies. This can have significant implications for generating polymer-nanoparticle theranostic systems that maintain therapeutic (e.g., nucleic acid) delivery efficacies, while also possessing additional functionalities, including

scholarly journals Glucose-Responsive Gene Delivery at Physiological pH through Tertiary-Amine Stabilized Boronate-PVA Particles Synthesized by One-Pot Reaction

Pharmaceutics ◽  
2021 ◽  
Vol 13 (1) ◽  
pp. 62
Author(s):  
Mangesh Morey ◽  
Akshay Srivastava ◽  
Abhay Pandit
Keyword(s):  
Gene Delivery ◽  
Fold Increase ◽  
Nonviral Gene Delivery ◽  
Luciferase Reporter ◽  
Hydrodynamic Diameter ◽  
Luciferase Reporter Gene ◽  
One Pot ◽  
Gaussia Luciferase ◽  
Nih3t3 Cells ◽  
The Stability

We report a physiologically stable and cytocompatible glucose-responsive nonviral gene delivery system made up of boronate functionalized polymeric material. Herein, we utilize boronate cis-diol interactions to develop a glucose-responsive submicron particle (SMP) system. The stability of the boronate interaction at a physiological pH was achieved by copolymerization of dimethyl aminoethyl methacrylate (DMAEMA) with acrylamidophenylboronic acid (AAPBA) and the formation of a complex with polyvinylalcohol (PVA) which is governed by cis-diol interactions. The shift in hydrodynamic diameter of SMPs was observed and correlated with increasing glucose concentrations at a physiological pH. Optimal transfection was observed for a 5 µg dose of the gaussia luciferase reporter gene in NIH3T3 cells without any adverse effect on cellular viability. The destabilization of the AAPBA–PVA complex by interacting with glucose allowed the release of encapsulated bovine serum albumin (BSA) in a glucose-responsive manner. In total, 95% of BSA was released from SMPs at a 50 mM glucose concentration after 72 h. A two-fold increase in transfection was observed in 50 mM glucose compared to that of 10 mM glucose.

Effect of Polymer Ionization on the Interaction with DNA in Nonviral Gene Delivery Systems

2003 ◽  
Vol 4 (3) ◽  
pp. 683-690 ◽  
Author(s):  
Uracha Rungsardthong ◽  
Touraj Ehtezazi ◽  
Lindsey Bailey ◽  
Steven P. Armes ◽  
Martin C. Garnett ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Delivery Systems ◽  
Nonviral Gene Delivery

scholarly journals The structure–function correlation analysed by OCT and full field ERG in typical and pericentral subtypes of retinitis pigmentosa

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Ching-Wen Huang ◽  
Jung-Je Yang ◽  
Chang-Hao Yang ◽  
Chung-May Yang ◽  
Fung-Rong Hu ◽  
...  
Keyword(s):  
Retinitis Pigmentosa ◽  
Structure Function ◽  
Subgroup Analysis ◽  
Primary Analysis ◽  
Cross Sectional ◽  
Zone Length ◽  
Full Field ◽  
Multiple Parameters ◽  
Typical Type ◽  
Function Correlation

AbstractTo investigate the structure–function correlation analysed by full-field electroretinography (ffERG) and optical coherence tomography (OCT) in typical and pericentral subtypes of retinitis pigmentosa (RP). A retrospective, cross-sectional, observational study of right eyes was conducted. The primary analysis used ffERG data to compare the RP subtypes. The subgroup analysis was used to correlate the structure, analysed by OCT, and function, determined by ffERG. Linear regressions explored the relationship between best-corrected visual acuity (BCVA) and multiple parameters. A total of 188 eyes were included. Amplitudes of responses of rod, rod-cone, cone, and 30 Hz flicker of typical type were lower than those of pericentral and other types. In the subgroup analysis, 41 and 21 eyes of the typical and pericentral types were studied, respectively. The correlation between the estimated preserved photoreceptor area and all ffERG amplitude parameters were significant in the typical type, but not in pericentral type. Old age, decreased intact ellipsoid zone length, typical type, and thin central retinal thickness were negatively correlated with BCVA. Typical type RP developed more extensive degeneration and poorer BCVA compared to others. Strong structure–function correlation was found in typical type while not in pericentral type. OCT may be a useful tool for monitoring RP status in typical type, providing useful parameters for the prediction of BCVA.

Photodegradable Neutral-Cationic Brush Block Copolymers for Nonviral Gene Delivery

2014 ◽  
Vol 9 (8) ◽  
pp. 2148-2155 ◽  
Author(s):  
Xianglong Hu ◽  
Yang Li ◽  
Tao Liu ◽  
Guoying Zhang ◽  
Shiyong Liu
Keyword(s):  
Block Copolymers ◽  
Gene Delivery ◽  
Nonviral Gene Delivery

scholarly journals A versatile system for receptor-mediated gene delivery permits increased entry of DNA into target cells, enhanced delivery to the nucleus and elevated rates of transgene expression

Gene Therapy ◽  
2000 ◽  
Vol 7 (15) ◽  
pp. 1337-1343 ◽  
Author(s):  
KD Fisher ◽  
K Ulbrich ◽  
V Subr ◽  
CM Ward ◽  
V Mautner ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Transgene Expression ◽  
Target Cells

scholarly journals An efficient nonviral gene-delivery vector based on hyperbranched cationic glycogen derivatives

2014 ◽  
pp. 419 ◽  
Author(s):  
Liqun Yang ◽  
Xuan Liang ◽  
Xianyue Ren ◽  
Zhenzhen Liu ◽  
Yingliang Liu ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Nonviral Gene Delivery ◽  
Gene Delivery Vector ◽  
Delivery Vector
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