Erste Erfahrungen mit dem kontinuierlichen Echtzeit- Glukosemonitoring-System (Guardian REAL-time CGMS) bei einer Katze mit Diabetes mellitus

2009 ◽  
Vol 151 (1) ◽  
pp. 27-30 ◽  
Author(s):  
S. Moretti ◽  
E. Zini ◽  
F. Tschuor ◽  
C.E. Reusch
Keyword(s):  
Diabetes Mellitus ◽  
Real Time

Correlation of microvascular abnormalities and endothelial dysfunction in Type-1 Diabetes Mellitus (T1DM): A real-time intravital microscopy study

2009 ◽  
Vol 42 (4) ◽  
pp. 285-295 ◽  
Author(s):  
Anthony T.W. Cheung ◽  
M. Meighan (Smith) Tomic ◽  
Peter C.Y. Chen ◽  
Eric Miguelino ◽  
Chin-Shang Li ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Type 1 Diabetes ◽  
Endothelial Dysfunction ◽  
Type 1 Diabetes Mellitus ◽  
Real Time ◽  
Intravital Microscopy ◽  
Microscopy Study

scholarly journals Quantitation of Gene Expression by Real-Time PCR Disproves a "Retroviral Hypothesis" for Childhood-Onset Diabetes Mellitus

1999 ◽  
Vol 46 (1) ◽  
pp. 57-60 ◽  
Author(s):  
Ina Knerr ◽  
Reinald Repp ◽  
Jörg Dötsch ◽  
Nils Gratzki ◽  
Jörg Hänze ◽  
...  
Keyword(s):  
Gene Expression ◽  
Diabetes Mellitus ◽  
Real Time ◽  
Real Time Pcr ◽  
Childhood Onset ◽  
Onset Diabetes

Correlation between Real-Time Microvascular Abnormalities and Whole Blood Viscosity (WBV) in Type-2 Diabetes Mellitus (T2DM) Patients.

Blood ◽  
2004 ◽  
Vol 104 (11) ◽  
pp. 3909-3909
Author(s):  
Anthony T. Cheung ◽  
Sahana Ramanujam ◽  
Michelle A. Barbosa ◽  
Violet K. Asfour ◽  
Maria V. Medina ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Early Detection ◽  
Real Time ◽  
Disease Severity ◽  
Time Window ◽  
Computer Assisted ◽  
Whole Blood Viscosity ◽  
Shear Rates ◽  
Control Subjects

Abstract Novel technologies were used to study the microvascular abnormalities in Type-2 diabetes mellitus (T2DM) patients and to correlate these real-time pathological complications with whole blood viscosity (WBV) over a wide range of shear rates (1–1,000s−1). Computer-assisted intravital microscopy (CAIM) was used to non-invasively and objectively quantify the microvascular abnormalities in the conjunctival microcirculation in T2DM patients (n=12; age range = 45 to 68) and healthy non-diabetic control subjects, with the medical history of each subject blinded to the investigators. Fifteen recognizable microvascular abnormalities existed in T2DM patients and not in the control subjects, though not all the abnormalities were found in each patient. A severity index (SI) -- the arithmetic sum of microvascular abnormalities in each patient quantified via CAIM -- was computed to give a score to correlate with medical history, WBV and shear rates. T2DM SI (6.9±1.7) differed significantly (P<0.01) from control SI (0.6±0.7) and correlated significantly with disease severity, but not with the duration of the disease since diagnosis. The results, together with results from a previous study, lend support to the hypothesis that diabetic complications may have occurred in the pre-diabetic period; indicative of the existence of a “time window” before the onset of clinically detectable hyperglycemia. To adequately assess WBV to correlate with T2DM SI, a computer-assisted scanning capillary viscometer, the Rheolog™, was used to generate a viscosity profile for each patient or control subject over a range of shear rates (1 to 1,000s−1) using one 3-ml citrated venous blood sample from each subject. Based on the viscosity profiles generated, WBV of T2DM patients at various shear rates differed significantly from control values. For example, averaged WBV of T2DM patients at a shear rate of 300s−1 (4.01±0.33cp) differed significantly (P<0.02) from averaged WBV of control subjects (3.34±0.05cp). WBV correlated with SI of T2DM patients, disease severity and medical records. This correlation may have unique clinical implications - it can be used to study shear-stress induced pathogenesis on endothelial dysfunction in various vascular diseases and in early detection of T2DM in carriers (e.g. siblings of T2DM patients) before clinical diagnosis of T2DM during the pre-diabetic “time window” of early detection. Follow-up studies on the correlation between real-time microvascular abnormalities (SI), disease severity, WBV and shear rates in Alzheimer’s Disease patients and in early detection of T2DM are in progress.

scholarly journals The rs1893217 (T/C) polymorphism in PTPN2 gene is not associated with type 1 diabetes mellitus in subjects from Southern Brazil

2014 ◽  
Vol 58 (4) ◽  
pp. 382-388 ◽  
Author(s):  
Jakeline Rheinheimer ◽  
Fernanda dos Santos de Oliveira ◽  
Luís Henrique Canani ◽  
Daisy Crispim
Keyword(s):  
Diabetes Mellitus ◽  
Type 1 Diabetes ◽  
Type 1 Diabetes Mellitus ◽  
Real Time ◽  
Real Time Pcr ◽  
Caucasian Population ◽  
Southern Brazil ◽  
Genotype Frequencies ◽  
Additive Inheritance

Objective: To evaluate the association of the PTPN2 rs1893217 polymorphism with T1DM and/or its clinical and laboratory characteristics in a Caucasian population from Southern Brazil. Subjects and methods: Four hundred and eighty six patients with T1DM and 484 non-diabetic subjects were included in the study. Genotyping of the PTPN2 rs1893217 was performed by real-time PCR. Results: Genotype frequencies did not differ between T1DM patients and non-diabetic subjects (P = 0.265). The C allele was observed in 14.5% of the T1DM sample and 12.2% of the non-diabetic group (P = 0.152). Moreover, the frequencies of this variant did not differ statistically between T1DM patients and non-diabetic subjects when assuming recessive, dominant, or additive inheritance models. The clinical and laboratory characteristics of T1DM patients did not differ significantly among the three genotypes of the rs1893217 polymorphism, either. Conclusion: The PTPN2 rs1893217 polymorphism is not significantly associated with T1DM in Caucasian subjects from Southern Brazil.

scholarly journals Fuyuan Xingnao Decoction Promotes Angiogenesis Through the Rab1/AT1R Pathway in Diabetes Mellitus Complicated With Cerebral Infarction

2021 ◽  
Vol 12 ◽  
Author(s):  
Dong Deng ◽  
Yao Qu ◽  
Lihua Sun ◽  
Liyang Jia ◽  
Jianhong Bu ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Cerebral Cortex ◽  
Cerebral Infarction ◽  
Real Time ◽  
Control Group ◽  
Ang Ii ◽  
Cerebral Microvessels ◽  
Expression Levels

Fuyuan Xingnao decoction (FYXN), a traditional Chinese formula comprised of seven herbs, has been utilized to treat diabetes mellitus complicated with cerebral infarction (DMCI) for years. Yet, its protective and regulatory mechanism is poorly understood. The aim of the study is to investigate the effects of FYXN on DMCI in vitro and in vivo, as well as its mechanism in angiogenesis. For in vivo experiments, FYXN was administered to DMCI rats with streptozotocin (STZ) injection-induced diabetes. Then middle cerebral artery occlusion (MCAO) was conducted and the cerebral cortex sections of the rats were obtained. The ultrastructure of cerebral microvessels and new vessel density of ischemic penumbra were evaluated by the transmission electron microscopy (TEM) assay and immunohistochemistry, respectively. Protein and mRNA expression levels of Rab1/AT1R in cortex were assayed by Western blotting and real-time fluorescence quantitative real-time polymerase chain reaction (RT-qPCR). In vitro, FYXN serum was produced in rats on the fourth day 2 h after the last FYXN administration. Green fluorescence was observed after transfection with lentivirus packaged Rab1-WT or siRNA for 24 h. The activity of brain microvascular endothelial cells (BMECs) treated with sera from these rats was tested by MTT assay and Transwell assays, respectively. The expression of AT1R on the cell membrane and endoplasmic reticulum of BMECs was evaluated by immunofluorescence staining. Protein expression levels of signaling molecules in the Rab1/AT1R pathways were also detected. Results showed that in vivo, FYXN treatment significantly intensified CD31 staining in the cortical areas and enhanced the mRNA and protein levels of AT1R, Ang II, Rab1a, Rab1b and VEGF expression in ischemic cerebral cortex tissues. In vitro, the expression levels of AT1R, Ang II, Rab1a, Rab1b and VEGF in the cerebral infarction model group were significantly higher than those in the control group, with further increases after administration of FYXN drug serum. FYXN promoted the proliferation and migration of BMECs by activating the Rab1/AT1R signaling pathway. In conclusion, FYXN exerts a protective effect against DMCI by promoting angiogenesis via the Rab1/AT1R pathway, which provides strong evidence for the therapeutic effect of FYXN on DMCI.

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