scholarly journals Erratum: The Arabidopsis Spontaneous Cell Death1 gene, encoding a ζ-carotene desaturase essential for carotenoid biosynthesis, is involved in chloroplast development, photoprotection and retrograde signalling

Cell Research ◽  
2007 ◽  
Vol 17 (6) ◽  
pp. 575-575 ◽  
Author(s):  
Haili Dong ◽  
Yan Deng ◽  
Jinye Mu ◽  
Qingtao Lu ◽  
Yiqin Wang ◽  
...  
Keyword(s):  
Chloroplast Development ◽  
Carotenoid Biosynthesis ◽  
Gene Encoding ◽  
Retrograde Signalling

scholarly journals Genome communication in plants mediated by organelle–n­ucleus-located proteins

2020 ◽  
Vol 375 (1801) ◽  
pp. 20190397 ◽  
Author(s):  
Karin Krupinska ◽  
Nicolás E. Blanco ◽  
Svenja Oetke ◽  
Michela Zottini
Keyword(s):  
Chloroplast Development ◽  
Cell Cycle Control ◽  
Plastid Transformation ◽  
Light Responses ◽  
Dual Targeting ◽  
Dual Localization ◽  
Retrograde Signalling ◽  
Eukaryotic Proteins ◽  
Plastid Proteins ◽  
Associated Proteins

An increasing number of eukaryotic proteins have been shown to have a dual localization in the DNA-containing organelles, mitochondria and plastids, and/or the nucleus. Regulation of dual targeting and relocation of proteins from organelles to the nucleus offer the most direct means for communication between organelles as well as organelles and nucleus. Most of the mitochondrial proteins of animals have functions in DNA repair and gene expression by modelling of nucleoid architecture and/or chromatin. In plants, such proteins can affect replication and early development. Most plastid proteins with a confirmed or predicted second location in the nucleus are associated with the prokaryotic core RNA polymerase and are required for chloroplast development and light responses. Few plastid–nucleus-located proteins are involved in pathogen defence and cell cycle control. For three proteins, it has been clearly shown that they are first targeted to the organelle and then relocated to the nucleus, i.e. the nucleoid-associated proteins HEMERA and Whirly1 and the stroma-located defence protein NRIP1. Relocation to the nucleus can be experimentally demonstrated by plastid transformation leading to the synthesis of proteins with a tag that enables their detection in the nucleus or by fusions with fluoroproteins in different experimental set-ups. This article is part of the theme issue ‘Retrograde signalling from endosymbiotic organelles’.

scholarly journals Controlled Transcription of Regulator Gene carS by Tet-on or by a Strong Promoter Confirms Its Role as a Repressor of Carotenoid Biosynthesis in Fusarium fujikuroi

2020 ◽  
Vol 9 (1) ◽  
pp. 71
Author(s):  
Julia Marente ◽  
Javier Avalos ◽  
M. Carmen Limón
Keyword(s):  
Wild Strain ◽  
Ring Finger ◽  
Carotenoid Biosynthesis ◽  
Negative Regulator ◽  
Fusarium Fujikuroi ◽  
Structural Genes ◽  
Gene Encoding ◽  
In The Wild ◽  
Set Up

Carotenoid biosynthesis is a frequent trait in fungi. In the ascomycete Fusarium fujikuroi, the synthesis of the carboxylic xanthophyll neurosporaxanthin (NX) is stimulated by light. However, the mutants of the carS gene, encoding a protein of the RING finger family, accumulate large NX amounts regardless of illumination, indicating the role of CarS as a negative regulator. To confirm CarS function, we used the Tet-on system to control carS expression in this fungus. The system was first set up with a reporter mluc gene, which showed a positive correlation between the inducer doxycycline and luminescence. Once the system was improved, the carS gene was expressed using Tet-on in the wild strain and in a carS mutant. In both cases, increased carS transcription provoked a downregulation of the structural genes of the pathway and albino phenotypes even under light. Similarly, when the carS gene was constitutively overexpressed under the control of a gpdA promoter, total downregulation of the NX pathway was observed. The results confirmed the role of CarS as a repressor of carotenogenesis in F. fujikuroi and revealed that its expression must be regulated in the wild strain to allow appropriate NX biosynthesis in response to illumination.

Chloroplast Development at Low Temperatures Requires a Homolog of DIM1, a Yeast Gene Encoding the 18S rRNA Dimethylase

1998 ◽  
Vol 10 (5) ◽  
pp. 699
Author(s):  
James G. Tokuhisa ◽  
Perumal Vijayan ◽  
Kenneth A. Feldmann ◽  
John A. Browse
Keyword(s):  
Low Temperatures ◽  
18S Rrna ◽  
Chloroplast Development ◽  
Yeast Gene ◽  
Gene Encoding

scholarly journals Chloroplast Development at Low Temperatures Requires a Homolog of DIM1, a Yeast Gene Encoding the 18S rRNA Dimethylase

1998 ◽  
Vol 10 (5) ◽  
pp. 699-711 ◽  
Author(s):  
James G. Tokuhisa ◽  
Perumal Vijayan ◽  
Kenneth A. Feldmann ◽  
John A. Browse
Keyword(s):  
Low Temperatures ◽  
18S Rrna ◽  
Chloroplast Development ◽  
Yeast Gene ◽  
Gene Encoding

scholarly journals Markerless Gene Deletion with Cytosine Deaminase in Thermus thermophilus Strain HB27

2015 ◽  
Vol 82 (4) ◽  
pp. 1249-1255 ◽  
Author(s):  
Lei Wang ◽  
Jana Hoffmann ◽  
Hildegard Watzlawick ◽  
Josef Altenbuchner
Keyword(s):  
Gene Deletion ◽  
Thermus Thermophilus ◽  
Cytosine Deaminase ◽  
Carotenoid Biosynthesis ◽  
Kanamycin Resistance ◽  
Origin Of Replication ◽  
Resistance Marker ◽  
Gene Encoding ◽  
Content Type

ABSTRACTWe developed a counterselectable deletion system forThermus thermophilusHB27 based on cytosine deaminase (encoded bycodA) fromThermaerobacter marianensisDSM 12885 and the sensitivity ofT. thermophilusHB27 to the antimetabolite 5-fluorocytosine (5-FC). The deletion vector comprises the pUC18 origin of replication, a thermostable kanamycin resistance marker functional inT. thermophilusHB27, andcodAunder the control of a constitutive putative trehalose promoter fromT. thermophilusHB27. The functionality of the system was demonstrated by deletion of thebglTgene, encoding a β-glycosidase, and three carotenoid biosynthesis genes,CYP175A1,crtY, andcrtI, from the genome ofT. thermophilusHB27.

scholarly journals Role for Vitamin B12 in Light Induction of Gene Expression in the Bacterium Myxococcus xanthus

2002 ◽  
Vol 184 (8) ◽  
pp. 2215-2224 ◽  
Author(s):  
María Cervantes ◽  
Francisco J. Murillo
Keyword(s):  
Myxococcus Xanthus ◽  
Carotenoid Biosynthesis ◽  
Inducible Promoter ◽  
Binding Domain ◽  
Gene Encoding ◽  
The Family ◽  
Mutant Strains ◽  
Carotenoid Genes ◽  
Carotenoid Synthesis ◽  
Novel Protein

ABSTRACT A light-inducible promoter (PB) drives the carB operon (carotenoid genes) of the bacterium Myxococcus xanthus. A gene encoding a regulator of carotenoid biosynthesis was identified by studying mutant strains carrying a transcriptional fusion to PB and deletions in three candidate genes. Our results prove that the identified gene, named carA, codes for a repressor of the PB promoter in the dark. They also show that the carA gene product does not participate in the light activation of two other promoters connected with carotenoid synthesis or its regulation in M. xanthus. CarA is a novel protein consisting of a DNA-binding domain of the family of MerR helix-turn-helix transcriptional regulators, directly joined to a cobalamin-binding domain. In support of this, we report here that the presence of vitamin B12 or some other cobalamin derivatives is absolutely required for activation of the PB promoter by light.

scholarly journals Genome Mining Reveals Two Missing CrtP and AldH Enzymes in the C30 Carotenoid Biosynthesis Pathway in Planococcus faecalis AJ003T

Molecules ◽  
2020 ◽  
Vol 25 (24) ◽  
pp. 5892
Author(s):  
Jun Ho Lee ◽  
Jin Won Kim ◽  
Pyung Cheon Lee
Keyword(s):  
Escherichia Coli ◽  
Biosynthetic Pathway ◽  
Genome Mining ◽  
Carotenoid Biosynthesis ◽  
Biosynthesis Pathway ◽  
Gene Encoding ◽  
A Genome ◽  
Genes Encoding ◽  
Carotenoid Biosynthesis Pathway ◽  
Carotenoid Pathway

Planococcus faecalis AJ003T produces glycosyl-4,4′-diaponeurosporen-4′-ol-4-oic acid as its main carotenoid. Five carotenoid pathway genes were presumed to be present in the genome of P. faecalis AJ003T; however, 4,4-diaponeurosporene oxidase (CrtP) was non-functional, and a gene encoding aldehyde dehydrogenase (AldH) was not identified. In the present study, a genome mining approach identified two missing enzymes, CrtP2 and AldH2454, in the glycosyl-4,4′-diaponeurosporen-4′-ol-4-oic acid biosynthetic pathway. Moreover, CrtP2 and AldH enzymes were functional in heterologous Escherichia coli and generated two carotenoid aldehydes (4,4′-diapolycopene-dial and 4,4′-diaponeurosporene-4-al) and two carotenoid carboxylic acids (4,4′-diaponeurosporenoic acid and 4,4′-diapolycopenoic acid). Furthermore, the genes encoding CrtP2 and AldH2454 were located at a distance the carotenoid gene cluster of P. faecalis.

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