scholarly journals Exploiting the Intron-splicing Mechanism of Insect Cells to Produce Viral Vectors Harboring Toxic Genes for Suicide Gene Therapy

2012 ◽  
Vol 1 ◽  
pp. e57 ◽  
Author(s):  
Haifeng Chen
Keyword(s):  
Gene Therapy ◽  
Viral Vectors ◽  
Insect Cells ◽  
Suicide Gene ◽  
Suicide Gene Therapy ◽  
Intron Splicing

scholarly journals The war against cancer: Suicide gene therapy

2016 ◽  
Vol 2 (3) ◽  
pp. 139
Author(s):  
Muzeyyen Izmirli ◽  
Dilara Sonmez ◽  
Bulent Gogebakan
Keyword(s):  
Gene Therapy ◽  
Cytochrome P450 ◽  
Side Effects ◽  
Cancer Cells ◽  
Thymidine Kinase ◽  
Viral Vectors ◽  
Suicide Gene ◽  
Suicide Gene Therapy ◽  
Kinase Gene ◽  
Cancer Society

<p>The National Cancer Institute and the American Cancer Society announced that 1.6 million new cancer cases are projected to occur in the USA in 2016. One of the most innovative approaches against cancer is suicide gene therapy, in which suicide-inducing transgenes are introduced into cancer cells. When cancer treatments target the total elimination of tumor cells, there will be no side effects for normal cells. Cancer tissues are targeted through various targeted transport methods, followed by tissue-specific enzymes converting a systemically suitable prodrug into an active drug in the tumor. Suicidal genes are delivered by transporters, such as viral and non-viral vectors, into cancer cells. Suicide gene therapeutic strategies currently pursued are herpes simplex virus thymidine kinase gene with prodrug ganciclovir, cytosine deaminase gene, carboxyl esterase/irinotecan, varicella zoster virus thymidine kinase/6-methoxypurine arabinonucleoside, nitroreductase Nfsb/5-(aziridin-1-yl)-2,4-dinitrobenzamide, carboxypeptidase G2/4-[(2-chloroethyl)(2- mesyloxyethyl)amino]benzoyl-L-glutamic acid, cytochrome p450-isofosfamide, and cytochrome p450-cyclophosphamide. The goal of this review is to summarize the different suicide gene systems and gene delivery vectors addressed to cancer cells, with a particular emphasis on recently developed systems. Finally, we briefly describe the advantageous clinical applications and potential side effects of suicide gene therapy. </p>

Gene delivery to carcinoma cells via novel non-viral vectors: Nanoparticle tracking analysis and suicide gene therapy

2014 ◽  
Vol 60 ◽  
pp. 72-79 ◽  
Author(s):  
Senait Gebremedhin ◽  
Aruna Singh ◽  
Stephen Koons ◽  
William Bernt ◽  
Krystyna Konopka ◽  
...  
Keyword(s):  
Gene Therapy ◽  
Gene Delivery ◽  
Viral Vectors ◽  
Suicide Gene ◽  
Suicide Gene Therapy ◽  
Carcinoma Cells ◽  
Nanoparticle Tracking Analysis

Suicide gene therapy may be effective in the treatment of malignant glioma

2021 ◽  
Author(s):  
Moataz Dowaidar
Keyword(s):  
Stem Cells ◽  
Gene Therapy ◽  
Stem Cell ◽  
Malignant Glioma ◽  
Viral Vectors ◽  
Glioma Cells ◽  
Suppressor Cells ◽  
Suicide Gene ◽  
Suicide Gene Therapy ◽  
Survival Ratio

Suicide gene therapy has a long history, and its effectiveness is now under investigation. The bystander death effect therapeutic strategy proved successful in treating malignant glioma; nevertheless, non-replicating viral vectors may not be able to cover a large invading region of glioma cells. Tumor-trophic stem cell migration and viral replication capacities have the potential to overcome the refractory mechanisms of malignant glioma. Surprisingly, the time to response (partial or total response) was delayed for more than 6 months after the initial Toca 511 treatment, reflecting a stronger antitumor immune response. Immunosuppressive cells such myeloid-derived suppressor cells and tumor-associated macrophages were found to be lower in human glioblastoma tissues after treatment. These mechanisms may have a long-term impact on people with malignant glioma. NSCs and MSCs, for example, might be employed to cover glioma cells' more broad invading areas. However, we must investigate which brain stem cells are the most suited. More study is needed to confirm the migratory potential and survival ratio of the implanted stem cells in the brain utilizing comparative analysis. Suicide gene therapy using stem cell migratory capability and/or viral replicating capability for malignant glioma may recapture the spotlight.

scholarly journals Using intron splicing trick for preferential gene expression in transduced cells: an approach for suicide gene therapy

2015 ◽  
Vol 23 (1) ◽  
pp. 7-12 ◽  
Author(s):  
F Pourzadegan ◽  
L Shariati ◽  
R Taghizadeh ◽  
H Khanahmad ◽  
Z Mohammadi ◽  
...  
Keyword(s):  
Gene Expression ◽  
Gene Therapy ◽  
Suicide Gene ◽  
Suicide Gene Therapy ◽  
Intron Splicing

Nanoparticle‐Mediated Suicide Gene Therapy for Triple Negative Breast Cancer Treatment

2020 ◽  
Vol 3 (8) ◽  
pp. 2000007
Author(s):  
Lucia Salvioni ◽  
Stefania Zuppone ◽  
Francesco Andreata ◽  
Matteo Monieri ◽  
Serena Mazzucchelli ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Gene Therapy ◽  
Cancer Treatment ◽  
Triple Negative Breast Cancer ◽  
Triple Negative ◽  
Suicide Gene ◽  
Breast Cancer Treatment ◽  
Suicide Gene Therapy

scholarly journals Development of iRGD-Modified Peptide Carriers for Suicide Gene Therapy of Uterine Leiomyoma

Pharmaceutics ◽  
2021 ◽  
Vol 13 (2) ◽  
pp. 202
Author(s):  
Anna Egorova ◽  
Sofia Shtykalova ◽  
Alexander Selutin ◽  
Natalia Shved ◽  
Marianna Maretina ◽  
...  
Keyword(s):  
Gene Therapy ◽  
Uterine Leiomyoma ◽  
Transfection Efficiency ◽  
Chemical Properties ◽  
Suicide Gene ◽  
Suicide Gene Therapy ◽  
Benign Tumors ◽  
Αvβ3 Integrin ◽  
Peptide Carrier ◽  
Plasmid Delivery

Uterine leiomyoma (UL) is one of the most common benign tumors in women that often leads to many reproductive complications. Suicide genetherapy was suggested as a promising approach for UL treatment. In the present study, we describe iRGD ligand-conjugated cysteine-rich peptide carrier RGD1-R6 for targeted DNA delivery to αvβ3 integrin-expressing primary UL cells. The physico-chemical properties, cytotoxicity, transfection efficiency and specificity of DNA/RGD1-R6 polyplexes were investigated. TheHSV-1thymidine kinase encoding plasmid delivery to PANC-1pancreatic carcinoma cells and primary UL cells resulted in significant suicide gene therapy effects. Subsequent ganciclovir treatment decreased cells proliferative activity, induced of apoptosis and promoted cells death.The obtained results allow us to concludethatthe developed RGD1-R6 carrier can be considered a promising candidate for suicide gene therapy of uterine leiomyoma.

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