scholarly journals Steady-state cross-correlations for live two-colour super-resolution localization data sets

2015 ◽  
Vol 6 (1) ◽  
Author(s):  
Matthew B. Stone ◽  
Sarah L. Veatch

Abstract Cross-correlation of super-resolution images gathered from point localizations allows for robust quantification of protein co-distributions in chemically fixed cells. Here this is extended to dynamic systems through an analysis that quantifies the steady-state cross-correlation between spectrally distinguishable probes. This methodology is used to quantify the co-distribution of several mobile membrane proteins in both vesicles and live cells, including Lyn kinase and the B-cell receptor during antigen stimulation.

2021 ◽  
Vol 504 (2) ◽  
pp. 2911-2923
Author(s):  
Arka Banerjee ◽  
Tom Abel

ABSTRACT Cross-correlations between data sets are used in many different contexts in cosmological analyses. Recently, k-nearest neighbour cumulative distribution functions (kNN-CDF) were shown to be sensitive probes of cosmological (auto) clustering. In this paper, we extend the framework of NN measurements to describe joint distributions of, and correlations between, two data sets. We describe the measurement of joint kNN-CDFs, and show that these measurements are sensitive to all possible connected N-point functions that can be defined in terms of the two data sets. We describe how the cross-correlations can be isolated by combining measurements of the joint kNN-CDFs and those measured from individual data sets. We demonstrate the application of these measurements in the context of Gaussian density fields, as well as for fully non-linear cosmological data sets. Using a Fisher analysis, we show that measurements of the halo-matter cross-correlations, as measured through NN measurements are more sensitive to the underlying cosmological parameters, compared to traditional two-point cross-correlation measurements over the same range of scales. Finally, we demonstrate how the NN cross-correlations can robustly detect cross-correlations between sparse samples – the same regime where the two-point cross-correlation measurements are dominated by noise.


1995 ◽  
Vol 31 (2) ◽  
pp. 193-204 ◽  
Author(s):  
Koen Grijspeerdt ◽  
Peter Vanrolleghem ◽  
Willy Verstraete

A comparative study of several recently proposed one-dimensional sedimentation models has been made. This has been achieved by fitting these models to steady-state and dynamic concentration profiles obtained in a down-scaled secondary decanter. The models were evaluated with several a posteriori model selection criteria. Since the purpose of the modelling task is to do on-line simulations, the calculation time was used as one of the selection criteria. Finally, the practical identifiability of the models for the available data sets was also investigated. It could be concluded that the model of Takács et al. (1991) gave the most reliable results.


2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Andrea de Almeida Brito ◽  
Heráclio Alves de Araújo ◽  
Gilney Figueira Zebende

AbstractDue to the importance of generating energy sustainably, with the Sun being a large solar power plant for the Earth, we study the cross-correlations between the main meteorological variables (global solar radiation, air temperature, and relative air humidity) from a global cross-correlation perspective to efficiently capture solar energy. This is done initially between pairs of these variables, with the Detrended Cross-Correlation Coefficient, ρDCCA, and subsequently with the recently developed Multiple Detrended Cross-Correlation Coefficient, $${\boldsymbol{DM}}{{\boldsymbol{C}}}_{{\bf{x}}}^{{\bf{2}}}$$DMCx2. We use the hourly data from three meteorological stations of the Brazilian Institute of Meteorology located in the state of Bahia (Brazil). Initially, with the original data, we set up a color map for each variable to show the time dynamics. After, ρDCCA was calculated, thus obtaining a positive value between the global solar radiation and air temperature, and a negative value between the global solar radiation and air relative humidity, for all time scales. Finally, for the first time, was applied $${\boldsymbol{DM}}{{\boldsymbol{C}}}_{{\bf{x}}}^{{\bf{2}}}$$DMCx2 to analyze cross-correlations between three meteorological variables at the same time. On taking the global radiation as the dependent variable, and assuming that $${\boldsymbol{DM}}{{\boldsymbol{C}}}_{{\bf{x}}}^{{\bf{2}}}={\bf{1}}$$DMCx2=1 (which varies from 0 to 1) is the ideal value for the capture of solar energy, our analysis finds some patterns (differences) involving these meteorological stations with a high intensity of annual solar radiation.


Nanophotonics ◽  
2020 ◽  
Vol 9 (9) ◽  
pp. 2847-2859
Author(s):  
Soojung Kim ◽  
Hyerin Song ◽  
Heesang Ahn ◽  
Seung Won Jun ◽  
Seungchul Kim ◽  
...  

AbstractAnalysing dynamics of a single biomolecule using high-resolution imaging techniques has been had significant attentions to understand complex biological system. Among the many approaches, vertical nanopillar arrays in contact with the inside of cells have been reported as a one of useful imaging applications since an observation volume can be confined down to few-tens nanometre theoretically. However, the nanopillars experimentally are not able to obtain super-resolution imaging because their evanescent waves generate a high optical loss and a low signal-to-noise ratio. Also, conventional nanopillars have a limitation to yield 3D information because they do not concern field localization in z-axis. Here, we developed novel hybrid nanopillar arrays (HNPs) that consist of SiO2 nanopillars terminated with gold nanodisks, allowing extreme light localization. The electromagnetic field profiles of HNPs are obtained through simulations and imaging resolution of cell membrane and biomolecules in living cells are tested using one-photon and 3D multiphoton fluorescence microscopy, respectively. Consequently, HNPs present approximately 25 times enhanced intensity compared to controls and obtained an axial and lateral resolution of 110 and 210 nm of the intensities of fluorophores conjugated with biomolecules transported in living cells. These structures can be a great platform to analyse complex intracellular environment.


Author(s):  
P. J. Bryanston-Cross ◽  
J. J. Camus

A simple technique has been developed which samples the dynamic image plane information of a schlieren system using a digital correlator. Measurements have been made in the passages and in the wakes of transonic turbine blades in a linear cascade. The wind tunnel runs continuously and has independently variable Reynolds and Mach number. As expected, strongly correlated vortices were found in the wake and trailing edge region at 50 KHz. Although these are strongly coherent we show that there is only limited cross-correlation from wake to wake over a Mach no. range M = 0.5 to 1.25 and variation of Reynolds number from 3 × 105 to 106. The trailing edge fluctuation cross correlations were extended both upstream and downstream and preliminary measurements indicate that this technique can be used to obtain information on wake velocity. The vortex frequency has also been measured over the same Mach number range for two different cascades. The results have been compared with high speed schlieren photographs.


Sign in / Sign up

Export Citation Format

Share Document