scholarly journals A naturally monomeric infrared fluorescent protein for protein labeling in vivo

2015 ◽  
Vol 12 (8) ◽  
pp. 763-765 ◽  
Author(s):  
Dan Yu ◽  
Michelle A Baird ◽  
John R Allen ◽  
Elizabeth S Howe ◽  
Matthew P Klassen ◽  
...  
Keyword(s):  
Fluorescent Protein ◽  
Protein Labeling ◽  
Infrared Fluorescent Protein

scholarly journals Quantitative assessment of near-infrared fluorescent proteins

2021 ◽  
Author(s):  
Kiryl Piatkevich ◽  
Hanbin Zhang ◽  
Stavrini Papadaki ◽  
Xiaoting Sun ◽  
Luxia Yao ◽  
...  
Keyword(s):  
Mammalian Cells ◽  
Quantitative Assessment ◽  
Near Infrared ◽  
Fluorescent Protein ◽  
Fluorescent Proteins ◽  
Oligomeric State ◽  
C Elegans ◽  
The Right ◽  
Infrared Fluorescent Protein

Abstract Recent progress in fluorescent protein development has generated a large diversity of near-infrared fluorescent proteins, which are rapidly becoming popular probes for a variety of imaging applications. To assist end-users with a selection of the right near-infrared fluorescent protein for a given application, we will conduct a quantitative assessment of intracellular brightness, photostability, and oligomeric state of 19 near-infrared fluorescent proteins in cultured mammalian cells. The top-performing proteins will be further validated for in vivo imaging of neurons in C. elegans, zebrafish, and mice. We will also assess the applicability of the selected NIR FPs for expansion microscopy and two-photon imaging.

scholarly journals Photoacoustic imaging of the near-infrared fluorescent protein iRFP in vivo

2012 ◽  
Author(s):  
Arie Krumholz ◽  
Grigory S. Filonov ◽  
Jun Xia ◽  
Junjie Yao ◽  
Vladislav V. Verkhusha ◽  
...  
Keyword(s):  
Near Infrared ◽  
Fluorescent Protein ◽  
Photoacoustic Imaging ◽  
Infrared Fluorescent Protein

scholarly journals Bright and stable near-infrared fluorescent protein for in vivo imaging

2011 ◽  
Vol 29 (8) ◽  
pp. 757-761 ◽  
Author(s):  
Grigory S Filonov ◽  
Kiryl D Piatkevich ◽  
Li-Min Ting ◽  
Jinghang Zhang ◽  
Kami Kim ◽  
...  
Keyword(s):  
In Vivo Imaging ◽  
Near Infrared ◽  
Fluorescent Protein ◽  
Infrared Fluorescent Protein

scholarly journals Non-invasive in vivo imaging of UCP1 expression in live mice via near-infrared fluorescent protein iRFP720

PLoS ONE ◽  
2019 ◽  
Vol 14 (11) ◽  
pp. e0225213 ◽  
Author(s):  
Aya Fukuda ◽  
Shiho Honda ◽  
Norie Fujioka ◽  
Yuya Sekiguchi ◽  
Seiya Mizuno ◽  
...  
Keyword(s):  
In Vivo Imaging ◽  
Near Infrared ◽  
Fluorescent Protein ◽  
Non Invasive ◽  
Ucp1 Expression ◽  
Infrared Fluorescent Protein

scholarly journals iRFP (near-infrared fluorescent protein) imaging of subcutaneous and deep tissue tumours in mice highlights differences between imaging platforms

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
C. Hall ◽  
Y. von Grabowiecki ◽  
S. P. Pearce ◽  
C. Dive ◽  
S. Bagley ◽  
...  
Keyword(s):  
Cancer Biology ◽  
Near Infrared ◽  
Fluorescent Protein ◽  
Dynamic Range ◽  
Fluorescent Proteins ◽  
Biological Tissues ◽  
The Body ◽  
Nsg Mice ◽  
Infrared Fluorescent Protein

Abstract Background In vivo imaging using fluorescence is used in cancer biology for the detection, measurement and monitoring of tumours. This can be achieved with the expression of fluorescent proteins such as iRFP, which emits light at a wavelength less attenuated in biological tissues compared to light emitted by other fluorescent proteins such as GFP or RFP. Imaging platforms capable of detecting fluorescent tumours in small animals have been developed but studies comparing the performance of these platforms are scarce. Results Through access to three platforms from Xenogen, Bruker and Li-Cor, we compared their ability to detect iRFP-expressing subcutaneous tumours as well as tumours localised deeper within the body of female NSG mice. Each platform was paired with proprietary software for image analyse, but the output depends on subjective decisions from the user. To more objectively compare platforms, we developed an ‘in house’ software-based approach which results in lower measured variability between mice. Conclusions Our comparisons showed that all three platforms allowed for reliable detection and monitoring of subcutaneous iRFP tumour growth. The biggest differences between platforms became apparent when imaging deeper tumours with the Li-Cor platform detecting most tumours and showing the highest dynamic range.

An individually coated near-infrared fluorescent protein as a safe and robust nanoprobe for in vivo imaging

Nanoscale ◽  
2013 ◽  
Vol 5 (21) ◽  
pp. 10345 ◽  
Author(s):  
Yu Yang ◽  
Kun Xiang ◽  
Yi-Xin Yang ◽  
Yan-Wen Wang ◽  
Xin Zhang ◽  
...  
Keyword(s):  
In Vivo Imaging ◽  
Near Infrared ◽  
Fluorescent Protein ◽  
Infrared Fluorescent Protein

scholarly journals Near-infrared fluorescent protein and bioluminescence-based probes for high-resolution in vivo optical imaging

2020 ◽  
Vol 1 (5) ◽  
pp. 967-987 ◽  
Author(s):  
Dhermendra K. Tiwari ◽  
Manisha Tiwari ◽  
Takashi Jin
Keyword(s):  
High Resolution ◽  
Optical Imaging ◽  
Recent Progress ◽  
Near Infrared ◽  
Fluorescent Protein ◽  
Imaging Techniques ◽  
Infrared Fluorescent Protein ◽  
In Vivo Optical Imaging

This review presents the recent progress on NIR fluorescent protein and bioluminescence-based probes with high-resolution in vivo imaging techniques.

scholarly journals Rationally designed fluorogenic protease reporter visualizes spatiotemporal dynamics of apoptosis in vivo

2015 ◽  
Vol 112 (11) ◽  
pp. 3338-3343 ◽  
Author(s):  
Tsz-Leung To ◽  
Beverly J. Piggott ◽  
Kalpana Makhijani ◽  
Dan Yu ◽  
Yuh Nung Jan ◽  
...  
Keyword(s):  
In Vivo Imaging ◽  
Fluorescent Protein ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Biological Applications ◽  
Protease Activation ◽  
Embryonic Morphogenesis ◽  
Infrared Fluorescent Protein ◽  
Spatiotemporal Coordination

Fluorescence resonance energy transfer-based reporters have been widely used in imaging cell signaling; however, their in vivo application has been handicapped because of poor signal. Although fluorogenic reporters overcome this problem, no such reporter of proteases has been demonstrated for in vivo imaging. Now we have redesigned an infrared fluorescent protein so that its chromophore incorporation is regulated by protease activity. Upon protease activation, the infrared fluorogenic protease reporter becomes fluorescent with no requirement of exogenous cofactor. To demonstrate biological applications, we have designed an infrared fluorogenic executioner-caspase reporter, which reveals spatiotemporal coordination between cell apoptosis and embryonic morphogenesis, as well as dynamics of apoptosis during tumorigenesis in Drosophila. The designed scaffold may be used to engineer reporters of other proteases with specific cleavage sequence.

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