scholarly journals Unexpected diversity among small-scale sample replicates of defined plant root compartments

2021 ◽  
Author(s):  
Sally Attia ◽  
Jakob Russel ◽  
Martin S. Mortensen ◽  
Jonas S. Madsen ◽  
Søren J. Sørensen

AbstractCommunity assembly processes determine patterns of species distribution and abundance which are central to the ecology of microbiomes. When studying plant root microbiome assembly, it is typical to sample at the whole plant root system scale. However, sampling at these relatively large spatial scales may hinder the observability of intermediate processes. To study the relative importance of these processes, we employed millimetre-scale sampling of the cell elongation zone of individual roots. Both the rhizosphere and rhizoplane microbiomes were examined in fibrous and taproot model systems, represented by wheat and faba bean, respectively. Like others, we found that the plant root microbiome assembly is mainly driven by plant selection. However, based on variability between replicate millimetre-scale samples and comparisons with randomized null models, we infer that either priority effects during early root colonization or variable selection among replicate plant roots also determines root microbiome assembly.

2007 ◽  
Vol 158 (8) ◽  
pp. 235-242 ◽  
Author(s):  
Hans Rudolf Heinimann

The term «precision forestry» was first introduced and discussed at a conference in 2001. The aims of this paper are to explore the scientific roots of the precision concept, define «precision forestry», and sketch the challenges that the implementation of this new concept may present to practitioners, educators, and researchers. The term «precision» does not mean accuracy on a small scale, but instead refers to the concurrent coordination and control of processes at spatial scales between 1 m and 100 km. Precision strives for an automatic control of processes. Precision land use differs from precision engineering by the requirements of gathering,storing and managing spatio-temporal variability of site and vegetation parameters. Practitioners will be facing the challenge of designing holistic, standardized business processes that are valid for whole networks of firms,and that follow available standards (e.g., SCOR, WoodX). There is a need to educate and train forestry professionals in the areas of business process re-engineering, computer supported management of business transactions,methods of remote sensing, sensor technology and control theory. Researchers will face the challenge of integrating plant physiology, soil physics and production sciences and solving the supply chain coordination problem (SCCP).


EcoHealth ◽  
2021 ◽  
Author(s):  
Felipe A. Hernández ◽  
Amanda N. Carr ◽  
Michael P. Milleson ◽  
Hunter R. Merrill ◽  
Michael L. Avery ◽  
...  

AbstractWe investigated the landscape epidemiology of a globally distributed mammal, the wild pig (Sus scrofa), in Florida (U.S.), where it is considered an invasive species and reservoir to pathogens that impact the health of people, domestic animals, and wildlife. Specifically, we tested the hypothesis that two commonly cited factors in disease transmission, connectivity among populations and abundant resources, would increase the likelihood of exposure to both pseudorabies virus (PrV) and Brucella spp. (bacterial agent of brucellosis) in wild pigs across the Kissimmee Valley of Florida. Using DNA from 348 wild pigs and sera from 320 individuals at 24 sites, we employed population genetic techniques to infer individual dispersal, and an Akaike information criterion framework to compare candidate logistic regression models that incorporated both dispersal and land cover composition. Our findings suggested that recent dispersal conferred higher odds of exposure to PrV, but not Brucella spp., among wild pigs throughout the Kissimmee Valley region. Odds of exposure also increased in association with agriculture and open canopy pine, prairie, and scrub habitats, likely because of highly localized resources within those land cover types. Because the effect of open canopy on PrV exposure reversed when agricultural cover was available, we suggest that small-scale resource distribution may be more important than overall resource abundance. Our results underscore the importance of studying and managing disease dynamics through multiple processes and spatial scales, particularly for non-native pathogens that threaten wildlife conservation, economy, and public health.


2016 ◽  
Vol 15 (1) ◽  
pp. 96
Author(s):  
E. Iglesias-Rodríguez ◽  
M. E. Cruz ◽  
J. Bravo-Castillero ◽  
R. Guinovart-Díaz ◽  
R. Rodríguez-Ramos ◽  
...  

Heterogeneous media with multiple spatial scales are finding increased importance in engineering. An example might be a large scale, otherwise homogeneous medium filled with dispersed small-scale particles that form aggregate structures at an intermediate scale. The objective in this paper is to formulate the strong-form Fourier heat conduction equation for such media using the method of reiterated homogenization. The phases are assumed to have a perfect thermal contact at the interface. The ratio of two successive length scales of the medium is a constant small parameter ε. The method is an up-scaling procedure that writes the temperature field as an asymptotic multiple-scale expansion in powers of the small parameter ε . The technique leads to two pairs of local and homogenized equations, linked by effective coefficients. In this manner the medium behavior at the smallest scales is seen to affect the macroscale behavior, which is the main interest in engineering. To facilitate the physical understanding of the formulation, an analytical solution is obtained for the heat conduction equation in a functionally graded material (FGM). The approach presented here may serve as a basis for future efforts to numerically compute effective properties of heterogeneous media with multiple spatial scales.


2021 ◽  
Vol 10 (3) ◽  
Author(s):  
Mathilde Nordgaard ◽  
Rasmus Møller Rosenbek Mortensen ◽  
Nikolaj Kaae Kirk ◽  
Ramses Gallegos‐Monterrosa ◽  
Ákos T. Kovács

2018 ◽  
Author(s):  
Gonzalo Duró ◽  
Alessandra Crosato ◽  
Maarten G. Kleinhans ◽  
Wim S. J. Uijttewaal

Abstract. Diverse methods are currently available to measure river bank erosion at broad-ranging temporal and spatial scales. Yet, no technique provides low-cost and high-resolution to survey small-scale bank processes along a river reach. We investigate the capabilities of Structure-from-Motion photogrammetry applied with imagery from an Unmanned Aerial Vehicle (UAV) to describe the evolution of riverbank profiles in middle-size rivers. The bank erosion cycle is used as a reference to assess the applicability of different techniques. We surveyed 1.2 km of a restored bank of the Meuse River eight times within a year, combining different photograph perspectives and overlaps to identify an efficient UAV flight to monitor banks. The accuracy of the Digital Surface Models (DSMs) was evaluated compared with RTK GPS points and an Airborne Laser Scanning (ALS) of the whole reach. An oblique perspective with eight photo overlaps was sufficient to achieve the highest relative precision to observation distance of ~1:1400, with 10 cm error range. A complementary nadiral view increased coverage behind bank toe vegetation. The DSM and ALS had comparable accuracies except on banks, where the latter overestimates elevations. Sequential DSMs captured signatures of the erosion cycle such as mass failures, slump-block deposition, and bank undermining. Although this technique requires low water levels and banks without dense vegetation, it is a low-cost method to survey reach-scale riverbanks in sufficient resolution to quantify bank retreat and identify morphological features of the bank failure and erosion processes.


Lab on a Chip ◽  
2021 ◽  
Author(s):  
Michel Moussus ◽  
Matthias Meier

High resolution live imaging promises new insights into the cellular and molecular dynamics of the plant root system in response to external cues. Microfluidic platforms are valuable analytical tools that...


2018 ◽  
Vol 2 (6) ◽  
pp. 1870051 ◽  
Author(s):  
Jayde A. Aufrecht ◽  
Collin M. Timm ◽  
Amber Bible ◽  
Jennifer L. Morrell-Falvey ◽  
Dale A. Pelletier ◽  
...  

2018 ◽  
Vol 22 (10) ◽  
pp. 5427-5444 ◽  
Author(s):  
Benjamin Mary ◽  
Luca Peruzzo ◽  
Jacopo Boaga ◽  
Myriam Schmutz ◽  
Yuxin Wu ◽  
...  

Abstract. The investigation of plant roots is inherently difficult and often neglected. Being out of sight, roots are often out of mind. Nevertheless, roots play a key role in the exchange of mass and energy between soil and the atmosphere, in addition to the many practical applications in agriculture. In this paper, we propose a method for roots imaging based on the joint use of two electrical noninvasive methods: electrical resistivity tomography (ERT) and mise-à-la-masse (MALM). The approach is based on the key assumption that the plant root system acts as an electrically conductive body, so that injecting electrical current into the plant stem will ultimately result in the injection of current into the subsoil through the root system, and particularly through the root terminations via hair roots. Evidence from field data, showing that voltage distribution is very different whether current is injected into the tree stem or in the ground, strongly supports this hypothesis. The proposed procedure involves a stepwise inversion of both ERT and MALM data that ultimately leads to the identification of electrical resistivity (ER) distribution and of the current injection root distribution in the three-dimensional soil space. This, in turn, is a proxy to the active (hair) root density in the ground. We tested the proposed procedure on synthetic data and, more importantly, on field data collected in a vineyard, where the estimated depth of the root zone proved to be in agreement with literature on similar crops. The proposed noninvasive approach is a step forward towards a better quantification of root structure and functioning.


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