scholarly journals Dissecting genetic diversity and genomic background of Petunia cultivars with contrasting growth habits

2020 ◽  
Vol 7 (1) ◽  
Author(s):  
Yufang Guo ◽  
Ryan M. Warner
Keyword(s):  
Genetic Diversity ◽  
Wild Species ◽  
Growth Habit ◽  
Morphological Variability ◽  
Flower Color ◽  
Genetic Origin ◽  
Breeding Programs ◽  
Cultivar Development ◽  
Growth Habits ◽  
Insight Into

Abstract The cultivated petunia (Petunia ×hybrida) is derived from the progenitor species P. axillaris and P. integrifolia. The hybridization dates back only to the 1830s, though intensive breeding efforts have yielded cultivars exhibiting incredible diversity for many traits, including growth habit, flower color, and flower size. Until now, little is known about the genetic diversity and genomic background of modern cultivars. Here we selected a panel of 13 cultivars with contrasting growth habits and three wild species (the progenitors and P. exserta) to estimate the genomic contribution from the ancestral species and to study whether the variation of the genetic origin could be associated with different breeding programs or morphological variability. Transcriptome sequencing identified 1,164,566 SNPs representing 98.4% (32,451) of the transcripts that cover 99.2% (of 52,697,361 bp) of the P. axillaris transcriptome. Cultivars with an upright growth habit had more homozygous alleles and more P. axillaris-derived alleles than trailing cultivars, while mounded cultivars had intermediate heterozygosity. Unlike previous studies, we found the proportions of alleles derived from each progenitor species varied across cultivars but overall were not biased toward one progenitor species, suggesting diverse selection during cultivar development. For trailing cultivars, alleles potentially introgressed from other wild species (“out” alleles) were enriched. The “out” alleles were clustered in particular regions of chromosomes, suggesting that these regions may be hotspots of introgression. Transcripts in these regions were enriched with gene ontology terms associated with growth habit. This study provides novel insight into the contributions of progenitor species to the genomic background of modern petunia cultivars and identifies genome regions that may harbor genes conferring the trailing growth habit for further exploration.

scholarly journals Genetic costs of domestication and improvement

2017 ◽  
Author(s):  
Brook T. Moyers ◽  
Peter L. Morrell ◽  
John K. McKay
Keyword(s):  
Wild Species ◽  
Genetic Variants ◽  
Deleterious Mutations ◽  
Evolutionary Processes ◽  
Breeding Programs ◽  
Plant Genomes ◽  
Genetic Gains ◽  
Number Frequency ◽  
The Cost ◽  
Insight Into

ABSTRACTThe ‘cost of domestication’ hypothesis posits that the process of domesticating wild species can result in an increase in the number, frequency, and/or proportion of deleterious genetic variants that are fixed or segregating in the genomes of domesticated species. This cost may limit the efficacy of selection and thus reduce genetic gains in breeding programs for these species. Understanding when and how deleterious mutations accumulate can also provide insight into fundamental questions about the interplay of demography and selection. Here we describe the evolutionary processes that may contribute to deleterious variation accrued during domestication and improvement, and review the available evidence for ‘the cost of domestication’ in animal and plant genomes. We identify gaps and explore opportunities in this emerging field, and finally offer suggestions for researchers and breeders interested in understanding or avoiding the consequences of an increased number or frequency of deleterious variants in domesticated species.

scholarly journals Short Communication: Genetic diversity of lemon (Citrus spp.) from Ternate Island (Indonesia) based on morphological and molecular characters

2020 ◽  
Vol 21 (5) ◽  
Author(s):  
Abdulrasyid Tolangara ◽  
ALOYSIUS DURAN COREBIMA ◽  
ABDU MAS’UD ◽  
SUNDARI
Keyword(s):  
Genetic Diversity ◽  
Morphological Character ◽  
Short Communication ◽  
Morphological Characters ◽  
Breeding Programs ◽  
Morphometric Characters ◽  
Insight Into ◽  
Citrus Spp ◽  
Molecular Characters ◽  
Selection Of

Abstract. Tolangara A, Corebima AD, Mas'ud A, Sundari. 2020. Short Communication: Genetic diversity of lemon (Citrus spp.) from Ternate Island (Indonesia) based on morphological and molecular characters. Biodiversitas 21: 1908-1913. One effort to build the image of Indonesian lemon can be by utilizing local genetic resources of Citrus spp. One of the growth centra lemon regions in North Maluku is Ternate Island. In Ternate island, cui (C. macrocarpa) is dominantly cultivated besides that there are Suanggi (C. histryx), nipis (C. aurantiifolia), and lemon (C sinensis). The genetic diversity of these local lemons in North Maluku, especially in Ternate, has not been scientifically reported. Therefore, this study aimed to analyze the genetic diversity of local lemons found on the island of Ternate based on morphological character and RAPD molecular analyses. A total of 12 accessions of local lemons in Ternate island to studied for morphological and molecular characters. Observation of morphological and morphometric characters of the lemons on 117 characters of vegetative and generative organs in lemons. Observation of molecular characters using three primers, i.e.: OPA1, OPA 2, and OPA 3 in the RAPD technique. The morphological character and molecular character using UPGMA analysis showed similar results. Ternate Island has moderate diversity of lemon species (75% based on morphological characters and 73% based on RAPD markers). Cluster analysis indicates that cui (C. macrocarpa) has the most distinctive autapomorphic character compared to the other local lemon varieties. Nipis (C.auratiifolia) and Suanggi (C. hystrix) shared 75% synapomorphic character similarity. This genetic diversity information provides a novel insight into the lemon commodity breeding programs which can be encouraged through the selection of superior local lemons based on morphological and molecular markers.

scholarly journals Molecular diversity in Lactucaspeciesusing Isozymes and RAPD Markers

2021 ◽  
Author(s):  
Reda H. Helmy Sammour ◽  
A-Z. A. Mustafa
Keyword(s):  
Genetic Diversity ◽  
Genetic Variation ◽  
Wild Species ◽  
Molecular Basis ◽  
Rapd Markers ◽  
Molecular Diversity ◽  
Breeding Programs ◽  
Primitive Form ◽  
In The Wild ◽  
Cultivated Species

Abstract Understanding of the molecular basis of genetic diversity in Lactucaaccessions is substantial for the management, improvementand efficient uses of Lactuca accessions. Therefore, this workaimed to evaluate molecular diversity among twenty-six accessions of Lactuca species usingisozymes and RAPD analyses. The polymorphic percentages were 87.09%and 100% in isozymes and RAPD analyses respectively, indicating a high genetic variation within and among Lactuca species. The number of alleles were higher in the wild species compared to the cultivated species, reflecting a reduction in the richness of alleles in the cultivated species due to domestication that caused a reduction in genetic diversity to meet the demand for high crop productivity.Isozymes and RAPD clustering dendrogrames: (1) separated,L. sativa accessions in more than one cluster confirming their polyphyletic origin; (2)collected the accessions of L. vimineain one cluster revealed its homogeneity; and (3) divided the accessions of L.saligna in two clusters varied in the number of alleles, particularly “A” form. The corresponding analysis associated the accessions of the wild species based on the alleles “B”of the tested isozymes and the cultivated species on alleles “A” and “C”, suggesting that: (1) allele “B” might be the primitive form of these loci that can tolerate the environmental stresses which prevails in the habitats of the wild species, and (2) “A” and “C” could be the derived forms. These results are of great interest for the management of Lactuca germplasm and for future breeding programs of lettuce.

Worldwide Genetic Diversity of the Wild Species Saccharum spontaneum and Level of Diversity Captured within Sugarcane Breeding Programs

Crop Science ◽  
2018 ◽  
Vol 58 (1) ◽  
pp. 218-229 ◽  
Author(s):  
Karen Aitken ◽  
Jingchuan Li ◽  
George Piperidis ◽  
Cai Qing ◽  
Fan Yuanhong ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Wild Species ◽  
Saccharum Spontaneum ◽  
Breeding Programs ◽  
Sugarcane Breeding

scholarly journals 283 BREAKUP OF LINKAGES FOR DIAGNOSTIC TRAITS IN THREE-SPECIES CONGRUITY BACKCROSS (CBC) PHASEOLUS HYBRIDS

HortScience ◽  
1994 ◽  
Vol 29 (5) ◽  
pp. 470e-470 ◽  
Author(s):  
Neil O. Anderson ◽  
Peter D. Ascher
Keyword(s):  
Growth Habit ◽  
Flower Color ◽  
Seed Morphology ◽  
Leaf Length ◽  
Hybrid Breakdown ◽  
Self Compatibility ◽  
Length Width ◽  
Growth Habits ◽  
Species Hybrids ◽  
Diagnostic Traits

Advanced, two-species CBC individuals were used to create the first-ever, three-species hybrids between P. acutifolius, P. coccineus and P. vulgaris. M6 (2 species) × H15 (3 species) is the only three-species hybrid to date that segregates for diagnostic traits. Three generations of M6 (F2, F3, F4,) were used to create the series. Hybrid breakdown was most severe with M6 F2 × H15, producing 100% cripples that died before anthesis. In M6 F3 × H15 hybrids, segregation for stigma position, flower color, germination type, growth habit, leaf length/width ratios, and seed morphology commenced in the F1 hybrid generation. F, phenotypes, with P. coccineus flowers & seeds and P. acutifolius leaves & growth habit, had severe hybrid breakdown with weak self compatibility; purple seed coats, with or without black circundatus markings, and new flower colors were also produced. F1's with P. vulgaris growth habits were self-fertile and ceased segregating after the F2.

scholarly journals Morphological Diversity and Bioactive Compounds in Wall Rocket (Diplotaxis erucoides (L.) DC.)

Agronomy ◽  
2020 ◽  
Vol 10 (2) ◽  
pp. 306 ◽  
Author(s):  
Carla Guijarro-Real ◽  
Jaime Prohens ◽  
Adrián Rodríguez-Burruezo ◽  
Ana Fita
Keyword(s):  
Growth Habit ◽  
Total Phenolics ◽  
Morphological Variability ◽  
Principal Component ◽  
Morphological Diversity ◽  
Breeding Programs ◽  
New Information ◽  
Diplotaxis Erucoides ◽  
Positive Correlations ◽  
Selection Of

Wall rocket is a wild vegetable with interest to become a crop. However, the information regarding morphological variability in the species is scarce, despite the interest it has received for breeding programs. In addition, evaluating the phytochemical composition can also be useful for developing materials of a high quality. In this study, forty-four populations were evaluated for selected morphoagronomic traits and contents in ascorbic acid (AA), total phenolics (TP), and nitrates (NO3−). Wall rocket plants had, on average, an intermediate growth habit and a good response to transplant. Moderate variability, mainly for size-related traits, was found, with low to moderate heritability estimates (H2 < 0.35). A Principal Component Analysis revealed that some materials may be selected for differenced traits. On the other hand, wall rocket materials had, on average, high contents in AA (53 mg 100 g−1) and TP (116 mg CAE 100 g−1) but also accumulated high levels of NO3− (891 mg 100 g−1). Significant positive correlations were found for AA and TP, which could be exploited for increasing the antioxidant activity and properties of the final product. We provide new information on the variation of wall rocket for traits of morphological and phytochemical interest, which together with other traits, such as the profile of glucosinolates, can be useful for the selection of materials in future breeding programs.

scholarly journals Molecular Diversity in Lactuca Species Using Isozymes and RAPD Markers

2021 ◽  
Author(s):  
Reda H. Helmy Sammour ◽  
A-Z. A. Mustafa ◽  
M. El-Esawi
Keyword(s):  
Genetic Diversity ◽  
Wild Species ◽  
Rapd Markers ◽  
Molecular Diversity ◽  
Crop Productivity ◽  
Breeding Programs ◽  
Primitive Form ◽  
Lactuca Species ◽  
In The Wild ◽  
Cultivated Species

Abstract Background: understanding molecular diversity in Lactuca species is substantial for the management, improvement and efficient uses of their accessions. Therefore, this work aimed to evaluate molecular diversity among and within Lactuca species. Methods and Results: the molecular diversity was assessed using isozymes and RAPD analyses that gave 87.09% and 100% polymorphic percentages respectively, indicating a high genetic variation within and among Lactuca species. The number of alleles were higher in the wild species compared to the cultivated species, reflecting a reduction in the richness of alleles in the cultivated species due to domestication that caused a reduction in genetic diversity to meet the demand for high crop productivity. Isozymes and RAPD clustering dendrogrames: (1) separated, L. sativa accessions in more than one cluster confirming their polyphyletic origin; (2) collected the accessions of L. viminea in one cluster revealed its homogeneity; and (3) divided the accessions of L. saligna in two clusters varied in the number of alleles, particularly “A” form. The corresponding analysis associated the accessions of the wild species based on “B” form of the alleles of the tested isozymes, and the cultivated species on the forms “A” and “C”, suggesting that: (1) allele “B” might be the primitive form of these loci that can tolerate the environmental stresses which prevails in the habitats of the wild species, and (2) forms “A” and “C” could be the derived forms. Conclusions: These results are of great interest for the management of Lactuca germplasm and for future breeding programs of lettuce.

Scanning Transmission Electron Microscopy of Polyethylene Crystals

1980 ◽  
Vol 38 ◽  
pp. 242-245
Author(s):  
F. Khoury ◽  
L. H. Bolz
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Crystallization Temperature ◽  
Scanning Transmission Electron Microscopy ◽  
Growth Habit ◽  
Lateral Growth ◽  
Scanning Transmission Electron ◽  
Transmission Electron ◽  
Scanning Transmission ◽  
Growth Habits

The lateral growth habits and non-planar conformations of polyethylene crystals grown from dilute solutions (<0.1% wt./vol.) are known to vary depending on the crystallization temperature.1-3 With the notable exception of a study by Keith2, most previous studies have been limited to crystals grown at <95°C. The trend in the change of the lateral growth habit of the crystals with increasing crystallization temperature (other factors remaining equal, i.e. polymer mol. wt. and concentration, solvent) is illustrated in Fig.l. The lateral growth faces in the lozenge shaped type of crystal (Fig.la) which is formed at lower temperatures are {110}. Crystals formed at higher temperatures exhibit 'truncated' profiles (Figs. lb,c) and are bound laterally by (110) and (200} growth faces. In addition, the shape of the latter crystals is all the more truncated (Fig.lc), and hence all the more elongated parallel to the b-axis, the higher the crystallization temperature.

scholarly journals Transcriptomic and chemical analyses to identify candidate genes involved in color variation of sainfoin flowers

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Yu Qiao ◽  
Qiming Cheng ◽  
Yutong Zhang ◽  
Wei Yan ◽  
Fengyan Yi ◽  
...  
Keyword(s):  
Regulatory Networks ◽  
Flower Color ◽  
Color Variation ◽  
Anthocyanin Synthesis ◽  
Flower Formation ◽  
Factors Affecting ◽  
Phenylpropanoid Biosynthesis ◽  
Molecular Information ◽  
Illumina Hiseq4000 ◽  
Insight Into

Abstract Background Sainfoin (Onobrychis viciifolia Scop) is not only a high-quality legume forage, but also a nectar-producing plant. Therefore, the flower color of sainfoin is an important agronomic trait, but the factors affecting its flower phenotype are still unclear. To gain insights into the regulatory networks associated with metabolic pathways of coloration compounds (flavonoids or anthocyanins) and identify the key genes, we conducted a comprehensive analysis of the phenotype, metabolome and transcriptome of WF and AF of sainfoin. Results Delphinidin, petunidin and malvidin derivatives were the main anthocyanin compounds in the AF of sainfoin. These substances were not detected in the WF of sainfoin. The transcriptomes of WF and AF in sainfoin at the S1 and S3 stages were obtained using the Illumina HiSeq4000 platform. Overall, 10,166 (4273 upregulated and 5893 downregulated) and 15,334 (8174 upregulated and 7160 downregulated) DEGs were identified in flowers at S1 and S3 stages, respectively (WF-VS-AF). KEGG pathway annotations showed that 6396 unigenes were annotated to 120 pathways and contained 866 DEGs at S1 stages, and 6396 unigenes were annotated to 131 pathways and included 1546 DEGs at the S3 stage. Nine DEGs belonging to the “flavonoid biosynthesis”and “phenylpropanoid biosynthesis” pathways involved in flower color formation were identified and verified by RT-qPCR analyses. Among these DEGs, 4CL3, FLS, ANS, CHS, DFR and CHI2 exhibited downregulated expression, and F3H exhibited upregulated expression in the WF compared to the AF, resulting in a decrease in anthocyanin synthesis and the formation of WF in sainfoin. Conclusions This study is the first to use transcriptome technology to study the mechanism of white flower formation in sainfoin. Our transcriptome data will be a great enrichment of the genetic information for sainfoin. In addition, the data presented herein will provide valuable molecular information for genetic breeding and provide insight into the future study of flower color polymorphisms in sainfoin.

scholarly journals Morphogenesis and evolution mechanisms of bacterially-induced struvite

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Tian-Lei Zhao ◽  
Han Li ◽  
Hao-Fan Jiang ◽  
Qi-Zhi Yao ◽  
Ying Huang ◽  
...  
Keyword(s):  
Time Course ◽  
Carboxyl Groups ◽  
Precipitation Process ◽  
Phosphate Content ◽  
Biomimetic Mineralization ◽  
Struvite Precipitation ◽  
Growth Habits ◽  
Two Stages ◽  
Relevant Work ◽  
Insight Into

AbstractBacteria are able to induce struvite precipitation, and modify struvite morphology, leading to the mineral with various growth habits. However, the relevant work involving the morphogenesis is limited, thereby obstructing our understanding of bacterially mediated struvite mineralization. Here, an actinomycete Microbacterium marinum sp. nov. H207 was chosen to study its effect on struvite morphology. A combination of bacterial mineralization and biomimetic mineralization techniques was adopted. The bacterial mineralization results showed that strain H207 could induce the formation of struvite with grouping structure (i.e., a small coffin-like crystal grown on a large trapezoid-like substrate crystal), and the overgrowth structure gradually disappeared, while the substrate crystal further evolved into coffin-like, and quadrangular tabular morphology with time. The biomimetic experiments with different organic components confirmed that the soluble macromolecules rich in electronegative carboxyl groups secreted by strain H207 dominate the formation of the struvite grouping. The time-course biomimetic experiments with supernatant testified that the increase in pH and NH4+ content promoted the evolution of crystal habits. Moreover, the evolution process of substrate crystal can be divided into two stages. At the first stage, the crystal grew along the crystallographic b axis. At the later stage, coupled dissolution–precipitation process occurred, and the crystals grew along the corners (i.e., [110] and [1-10] directions). In the case of dissolution, it was also found that the (00-1) face of substrate crystal preferentially dissolved, which results from the low initial phosphate content and high PO43− density on this face. As a result, present work can provide a deeper insight into bio-struvite mineralization.

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