scholarly journals Intratumoral generation of photothermal gold nanoparticles through a vectorized biomineralization of ionic gold

2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Aaron S. Schwartz-Duval ◽  
Christian J. Konopka ◽  
Parikshit Moitra ◽  
Enrique A. Daza ◽  
Indrajit Srivastava ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Polyethylene Glycol ◽  
Incubation Time ◽  
Biomedical Applications ◽  
Gold Clusters ◽  
Chloroauric Acid ◽  
Delivery Vector ◽  
Biomedical Potential ◽  
Ionic Gold

Abstract Various cancer cells have been demonstrated to have the capacity to form plasmonic gold nanoparticles when chloroauric acid is introduced to their cellular microenvironment. But their biomedical applications are limited, particularly considering the millimolar concentrations and longer incubation period of ionic gold. Here, we describe a simplistic method of intracellular biomineralization to produce plasmonic gold nanoparticles at micromolar concentrations within 30 min of application utilizing polyethylene glycol as delivery vector for ionic gold. We have characterized this process for intracellular gold nanoparticle formation, which progressively accumulates proteins as the ionic gold clusters migrate to the nucleus. This nano-vectorized application of ionic gold emphasizes its potential biomedical opportunities while reducing the quantity of ionic gold and required incubation time. To demonstrate its biomedical potential, we further induce in-situ biosynthesis of gold nanoparticles within MCF7 tumor mouse xenografts which is followed by its photothermal remediation.

Seed-mediated synthesis and PEG coating of gold nanoparticles for controlling morphology and sizes

MRS Advances ◽  
2020 ◽  
Vol 5 (63) ◽  
pp. 3353-3360
Author(s):  
Susana Helena Arellano Ramírez ◽  
Perla García Casillas ◽  
Christian Chapa González
Keyword(s):  
Gold Nanoparticles ◽  
Polyethylene Glycol ◽  
Room Temperature ◽  
Colloidal Stability ◽  
Infrared Spectrometry ◽  
Ammonium Bromide ◽  
Chloroauric Acid ◽  
Vis Spectroscopy ◽  
Seed Mediated ◽  
Peg Coating

AbstractA significant area of research is biomedical applications of nanoparticles which involves efforts to control the physicochemical properties through simple and scalable processes. Gold nanoparticles have received considerable attention due to their unique properties that they exhibit based on their morphology. Gold nanospheres (AuNSs) and nanorods (AuNRs) were prepared with a seed-mediated method followed of polyethylene glycol (PEG)-coating. The seeds were prepared with 0.1 M cetyltrimethyl-ammonium bromide (CTAB), 0.005 M chloroauric acid (HAuCl4), and 0.01 M sodium borohydride (NaBH4) solution. Gold nanoparticles with spherical morphology was achieved by growth by aggregation at room temperature, while to achieve the rod morphology 0.1 M silver nitrate (AgNO3) and 0.1 M ascorbic acid solution were added. The gold nanoparticles obtained by the seed-mediated synthesis have spherical or rod shapes, depending on the experimental conditions, and a uniform particle size. Surface functionalization was developed using polyethylene glycol. Morphology, and size distribution of AuNPs were evaluated by Field Emission Scanning Electron Microscopy. The average size of AuNSs, and AuNRs was 7.85nm and 7.96 x 31.47nm respectively. Fourier transform infrared spectrometry was performed to corroborate the presence of PEG in the AuNPs surface. Additionally, suspensions of AuNSs and AuNRs were evaluated by UV-Vis spectroscopy. Gold nanoparticles were stored for several days at room temperature and it was observed that the colloidal stability increased once gold nanoparticles were coated with PEG due to the shield formed in the surface of the NPs and the increase in size which were 9.65±1.90 nm of diameter for AuNSs and for AuNRs were 29.03±5.88 and 8.39±1.02 nm for length and transverse axis, respectively.

A facile method to in situ fabricate three dimensional gold nanoparticle micropatterns in a cell-resistant hydrogel

2016 ◽  
Vol 15 (2) ◽  
pp. 181-186
Author(s):  
Ming-Hao Yao ◽  
Jie Yang ◽  
Dong-Hui Zhao ◽  
Rui-Xue Xia ◽  
Rui-Mei Jin ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Polyethylene Glycol ◽  
Gold Nanoparticle ◽  
Three Dimensional ◽  
Photochemical Synthesis ◽  
A Cell

A facile method for in situ fabrication of three-dimensional gold nanoparticles micropatterns throughout a polyethylene glycol hydrogel substrate has been developed by combining photochemical synthesis of gold nanoparticles with photolithography technology.

Hybrid Microgels Containing Gold Nanoparticles

e-Polymers ◽  
2006 ◽  
Vol 6 (1) ◽  
Author(s):  
Andrij Pich ◽  
Arpita Karak ◽  
Yan Lu ◽  
Anup K. Ghosh ◽  
Hans-Juergen P. Adler
Keyword(s):  
Gold Nanoparticles ◽  
Sodium Citrate ◽  
Colloidal Stability ◽  
Nano Particles ◽  
Composite Particles ◽  
Temperature Sensitive ◽  
Chloroauric Acid ◽  
Gold Nano Particles ◽  
Hybrid Microgels

AbstractIn present paper we report on a preparation of hybrid microgels filled with gold nano-particles (AuNPs). Temperature-sensitive VCL/AAEM microgels have been used as containers for deposition of Au by in situ formation of gold nano-particles. Synthesis of AuNPs has been performed by two methods: a) reduction of chloroauric acid with sodium borhydride and b) reduction of chloroauric acid by sodium citrate under ultrasonic agitation. In both cases AuNPs were deposited directly into microgels leading to formation of composite particles which exhibit temperature-sensitive properties, tunable gold contents and high colloidal stability. The influence of Au load on microgel size, morphology, swellingdeswelling behaviour and stability is discussed.

Development of novel bioelectrocatalytic platform based on in situ generated gold nanoparticles for biomedical applications

2012 ◽  
Vol 1418 ◽  
Author(s):  
Prem C. Pandey ◽  
Dheeraj S. Chauhan
Keyword(s):  
Hydrogen Peroxide ◽  
Gold Nanoparticles ◽  
Biomedical Applications ◽  
Potassium Ferricyanide ◽  
Organically Modified Silicates ◽  
Organically Modified ◽  
Electrocatalytic Determination ◽  
Aunp Surface

ABSTRACTGold nanoparticles (AuNp) formed using alkoxysilane precursors are utilized in the development of thin organically modified silicates (ormosil) films. The resulting films are optically transparent thereby retaining the optical properties of AuNp. Surface morphology shows that the in situ generated AuNp retained their nanogeometry in the ormosil films. An application of the AuNp encapsulated ormosils is shown in electrocatalytic determination of hydrogen peroxide. For this purpose, potassium ferricyanide is chosen as electron transfer mediator and is encapsulated in the films. Results show that the presence of AuNp in the ormosil matrix dramatically improves the electrochemical behavior of potassium ferricyanide. The ormosil films are utilized for electrocatalytic determination of hydrogen peroxide. In order to investigate the biocompatibility of the ormosil film, horseradish peroxidase (HRP) is incorporated resulting in improvement in oxidation and reduction of peroxide.

scholarly journals Synthesis and Optical Properties of Colloidal Gold Nanoparticles for Biomedical Applications

2011 ◽  
Vol 21 (1) ◽  
pp. 63 ◽  
Author(s):  
Nghiem Thi Ha Lien ◽  
Vu Xuan Hoa ◽  
Vu Thi Thuy Duong ◽  
Nguyen Van Tinh ◽  
Tran Hong Nhung
Keyword(s):  
Gold Nanoparticles ◽  
Colloidal Gold ◽  
Biomedical Applications ◽  
Strong Dependence ◽  
Trisodium Citrate ◽  
Visible Range ◽  
Chloroauric Acid ◽  
Stabilizing Agents ◽  
Colloidal Gold Nanoparticles ◽  
The Stability

The gold nanoparticle solutions are well known as an extremely sensitive biomedical analytical tool due to the strong dependence of their absorption in the visible range on the environment. In this work, the colloidal nano golds were synthesized from metal precursor chloroauric acid (HAuCl4) using trisodium citrate dehydrate (C6H5O7Na3) as reducing and stabilizing agents. The optical characterization - absorption of colloidal gold solution have been investigated under the different synthetic conditions such as: reducing agent concentration, concentration of auric ion, pH, duration of reaction and aging time. The results show that the absorption and the stability of the colloidal gold nanoparticles depend robustly on the synthetic conditions and pH of environment.

In situ plasma-assisted synthesis of polydopamine-functionalized gold nanoparticles for biomedical applications

2020 ◽  
Vol 22 (19) ◽  
pp. 6588-6599
Author(s):  
Linh Nhat Nguyen ◽  
Neha Kaushik ◽  
Pradeep Lamichhane ◽  
Sohail Mumtaz ◽  
Ramhari Paneru ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Redox Reaction ◽  
Biomedical Applications ◽  
Functionalized Gold Nanoparticles

The green fabrication of polydopamine-functionalized gold nanoparticles by using plasma–liquid interaction to trigger a redox reaction and their biomedical applications.

Polymer Conjugated Gold Nanoparticles in Biomedical Applications

2018 ◽  
Vol 25 (12) ◽  
pp. 1433-1445 ◽  
Author(s):  
Stanley Anniebell ◽  
Subash C.B. Gopinath
Keyword(s):  
Gold Nanoparticles ◽  
Polyethylene Glycol ◽  
Gold Nanoparticle ◽  
Biomedical Applications ◽  
Molecular Level ◽  
Therapeutic Agents ◽  
Research Interest ◽  
Side Reactions ◽  
Low Levels ◽  
Specific Reactions

Background: Research interest on the properties of polymer conjugated gold nanoparticle (GNP) in biomedicine is rapidly rising because of the extensive evidences for their unique properties. In the field of biomedicine, GNPs have been widely used because of their inertness and low levels of cytotoxicity. Therefore, when exposed to cells, they are less prone to exert damaging effects. GNPs are capable of being functionalized as desired and are ideal as they do not encourage undesired side reactions that might counter react with the intention of the functionalization. Biofouling is an occurrence that takes place at cellular and biological molecular level, binds non-specifically on the detection surface and forms a wrong output. This undesired incidence can be avoided by conjugating the surface of biomolecules with polymers. Densely packed repeating chains of polymers such as polyethylene glycol are capable of decreasing non-specific reactions. Applications of polymer conjugated GNPs in the field of biomedicine are as biosensors, delivery and therapeutic agents. Conclusion: Therefore, the properties and applications of polymer conjugated GNPs are studied widely as overviewed here.

scholarly journals Evaluation of Gold Nanoparticles in Terms of Their Use in Biomedical Applications

2019 ◽  
Vol 7 (10) ◽  
pp. 1694
Author(s):  
Burcu Biterge-Süt ◽  
Elif Canpolat
Keyword(s):  
Antimicrobial Activity ◽  
Gold Nanoparticles ◽  
Physical Properties ◽  
Biomedical Applications ◽  
Salmonella Enteritidis ◽  
Biological Properties ◽  
Disk Diffusion ◽  
Diffusion Method ◽  
Chloroauric Acid ◽  
Disk Diffusion Method

Recently nanotechnology has become an integral part of modern biomedical applications. Accordingly, nanoparticles are considered as promising components for the development of innovative tags, probes, biosensors and carrier molecules for drug delivery. Spherical colloidal gold nanoparticles (AuNPs) are prime candidates to be utilized for these purposes due to their useful physical properties. However, in order for the gold nanoparticles to be used in nanomedicine, their biological properties should be extensively studied as well. Therefore, in this paper we chemically synthesized gold nanoparticles and studied their physical and biological characteristics to determine their potential use in medicine. Gold nanoparticles were synthesized by the reduction of chloroauric acid (HAuCl4) solution with sodium citrate. The physical properties of the AuNPs were determined by UV–vis spectrophotometer and Zetasizer readings. The antimicrobial activity of the newly synthesized gold nanoparticles on Escherichia coli, Salmonella infantis, Salmonella kentucky, Salmonella typhimurium, Salmonella enteritidis, Pseudomonas aeruginosa, Listeria monocytogenes, Staphylococcus aureus, Staphylococcus epidermidis, Enterococcus faecalis, Bacillus subtilis and Candida albicans were investigated via disk diffusion method. We found that the AuNPs were monodisperse, stable and not prone to aggregation with an average size of 22.12 nm and an emission band at 522 nm. The disk diffusion tests revealed that the gold nanoparticles did not have a significant growth inhibitory effect on the pathogens tested. In conclusion, here we showed the successful synthesis of gold nanoparticles by a safe and non-toxic method. Furthermore, our evaluation of the antimicrobial activity of these nanoparticles suggests that these molecules could be considered as biologically safe molecules for future medical applications.

Thermal gravimetric analysis of in-situ crosslinked nanocellulose whiskers • poly(methyl vinyl ether-co-maleic acid) • polyethylene glycol

TAPPI Journal ◽  
2011 ◽  
Vol 10 (4) ◽  
pp. 29-33
Author(s):  
LEE A. GOETZ ◽  
AJI P. MATHEW ◽  
KRISTIINA OKSMAN ◽  
ARTHUR J. RAGAUSKAS
Keyword(s):  
Thermal Stability ◽  
Polyethylene Glycol ◽  
Vinyl Ether ◽  
Thermal Gravimetric Analysis ◽  
Maleic Acid ◽  
Gravimetric Analysis ◽  
Thermal Gravimetric ◽  
Methyl Vinyl ◽  
Methyl Vinyl Ether

The thermal stability and decomposition of in-situ crosslinked nanocellulose whiskers – poly(methyl vinyl ether-co-maleic acid) – polyethylene glycol formulations (PMVEMA-PEG), (25%, 50%, and 75% whiskers) – were investigated using thermal gravimetric analysis (TGA) methods. The thermal degradation behavior of the films varied according to the percent cellulose whiskers in each formulation. The presence of cellulose whiskers increased the thermal stability of the PMVEMA-PEG matrix.

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