scholarly journals Aggregation-induced emission luminogens for image-guided surgery in non-human primates

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Danni Zhong ◽  
Weiyu Chen ◽  
Zhiming Xia ◽  
Rong Hu ◽  
Yuchen Qi ◽  
...  
Keyword(s):  
Animal Models ◽  
Biomedical Applications ◽  
Intraoperative Imaging ◽  
Surgical Excision ◽  
Surgical Operation ◽  
Pilot Studies ◽  
Aggregation Induced Emission ◽  
Guided Surgery ◽  
Tumor Accumulation ◽  
Human Primate

AbstractDuring the past two decades, aggregation-induced emission luminogens (AIEgens) have been intensively exploited for biological and biomedical applications. Although a series of investigations have been performed in non-primate animal models, there is few pilot studies in non-human primate animal models, strongly hindering the clinical translation of AIE luminogens (AIEgens). Herein, we present a systemic and multifaceted demonstration of an optical imaging-guided surgical operation via AIEgens from small animals (e.g., mice and rabbits) to rhesus macaque, the typical non-human primate animal model. Specifically, the folic conjugated-AIE luminogen (folic-AIEgen) generates strong and stable fluorescence for the detection and surgical excision of sentinel lymph nodes (SLNs). Moreover, with the superior tumor/normal tissue ratio and rapid tumor accumulation, folic-AIEgen successfully images and guides the precise resection of invisible cancerous metastases. Taken together, the presented strategies of folic-AIEgen based fluorescence intraoperative imaging and visualization-guided surgery show potential for clinical applications.

Animal Experiments and their Alternatives in Psychiatry

1988 ◽  
Vol 15 (4) ◽  
pp. 313-318
Author(s):  
Anthony Stevens
Keyword(s):  
Animal Models ◽  
Human Study ◽  
Animal Experiments ◽  
Experimental Production ◽  
Potential Utility ◽  
Pharmacological Agents ◽  
Wide Range ◽  
Influential Paper ◽  
Adequate Data ◽  
Human Primate

During the last twenty years, the most enthusiastic advocates of the use of animal models in the study of human psychiatric dysfunction have been Harlow and Suomi. In an influential paper, Induced Depression in Monkeys (1974), they argued that more extensive use of non-human primates “would have great potential utility since many manipulations and measurements presently prohibited in human study by ethical and practical considerations could be readily performed on non-human primate subjects in well-controlled experimental environments.” Harlow & Suomi concluded this paper with the following statement: “The results obtained to date on induced depression in monkeys show that proper and profound depressions can be produced relatively easily by a variety of techniques. These induced depressions either bear a close resemblance to human depression or have such similarity as to suggest that closely correlated human and animal depressive patterns may be achieved with refined techniques. The results to date also provide adequate data for the conduct of meaningful researches on the effects of pharmacological agents which either enhance, inhibit or preclude the experimental production of depression. Further, the existence of firm and fast monkey depression syndromes offers vast opportunities for testing a wide range of therapeutic techniques, either behavioural or biochemical.”

scholarly journals Translational Animal Models for Liver Cancer

2018 ◽  
Vol 2 ◽  
pp. 2 ◽  
Author(s):  
Michele Obeid ◽  
Ramzy C. Khabbaz ◽  
Kelly D. Garcia ◽  
Kyle M. Schachtschneider ◽  
Ron C. Gaba
Keyword(s):  
Hepatocellular Carcinoma ◽  
Cancer Research ◽  
Animal Models ◽  
Liver Cancer ◽  
Small Animal ◽  
Large Animal ◽  
Starting Point ◽  
Perfect Model ◽  
Small Animal Models ◽  
Human Primate

Animal models have become increasingly important in the study of hepatocellular carcinoma (HCC), as they serve as a critical bridge between laboratory-based discoveries and human clinical trials. Developing an ideal animal model for translational use is challenging, as the perfect model must be able to reproduce human disease genetically, anatomically, physiologically, and pathologically. This brief review provides an overview of the animal models currently available for translational liver cancer research, including rodent, rabbit, non-human primate, and pig models, with a focus on their respective benefits and shortcomings. While small animal models offer a solid starting point for investigation, large animal HCC models are becoming increasingly important for translation of preclinical results to clinical practice.

Abstract 2055: A Fingertip-Mounted Ultrasound Probe for Open-Chest Echocardiographic Imaging During Heart Surgery: Pilot Studies with a New Scanning Method

Circulation ◽  
2007 ◽  
Vol 116 (suppl_16) ◽  
Author(s):  
Ling Hui ◽  
Ronald W Schutz ◽  
Scott Corbett ◽  
Evan Dudik ◽  
Shelly Kinnune ◽  
...  
Keyword(s):  
Left Ventricle ◽  
Left Atrium ◽  
Heart Surgery ◽  
Intraoperative Imaging ◽  
Imaging Study ◽  
Ultrasound Probe ◽  
Pilot Studies ◽  
Scanning Method ◽  
Open Chest ◽  
Array Ultrasound

Background: We report a newly developed fingertip-mounted ultrasound probe (SonicEye®), which has been applied to an open-chest intraoperative imaging study. This probe is small, flexible, and quickly detachable. Its intuitive positioning makes it easy to acquire images in standard as well as unique views including views from the diaphragmatic surface of the left ventricle and behind the left atrium with a “heads up” that can be easily viewed by the surgeon while looking at the heart and the probe. Methods: In an open-chest study of two 12–14 kg piglets, the finger-mounted (11 mm radius, 4 – 6 MHz) and a conventional phased array ultrasound probe (Siemens 7V3C, 4 – 6 MHz) were used for scanning on a Siemens Cypress system. Images were recorded and analyzed for quality of image offline. Results: The fingertip-mounted ultrasound probe had the same performance as the conventional ultrasound probe as regards to image quality and Doppler performance. Furthermore, the probe could provide unique views from behind the left atrium and/or under the diaphragmatic surface of the left ventricle that even TEE does not match. Conclusions: The fingertip-mounted probe was able to capture high quality images during our study. This probe is easy to wear and provides high resolution unique images which should be useful for intraoperative evaluation of cardiac repairs.

scholarly journals A Simple Technique for Interventional Tool Placement Combining Fluoroscopy With Interventional Computed Tomography on a C-Arm System

2010 ◽  
Vol 67 (3) ◽  
pp. ons49-ons57 ◽  
Author(s):  
Brian E. Nett ◽  
Beverly Aagaard-Kienitz ◽  
Yurdal Serarslan ◽  
Mustafa K. Başkaya ◽  
Guang-Hong Chen
Keyword(s):  
Computed Tomography ◽  
Intraoperative Imaging ◽  
Cone Beam ◽  
Preoperative Imaging ◽  
Clinical Feature ◽  
Flat Panel ◽  
Guided Surgery ◽  
Surgical Tool ◽  
Contrast Enhanced ◽  
Needle Trajectory

Abstract BACKGROUND: Flat-panel cone-beam computed tomography (FP-CBCT) has recently been introduced as a clinical feature in neuroangiography radiographic C-arm systems. OBJECTIVE: To introduce a method of positioning a surgical tool such as a needle or ablation probe within a target specified by intraoperative FP-CBCT scanning. METHODS: Two human cadaver and 2 porcine cadaver heads were injected with a mixture of silicone and contrast agent to simulate a contrast-enhanced tumor. Preoperative imaging was performed using a standard 1.5-T magnetic resonance imaging scanner. Intraoperative imaging was used to define the needle trajectory on a GE Innova 4100 flat panel-based neuroangiography C-arm system. RESULTS: Using a combination of FP-CBCT and fluoroscopy, a needle was successfully positioned within each of the simulated contrast-enhanced tumors, as verified by subsequent FP-CBCT scans. CONCLUSIONS: This proof-of-concept study demonstrates the potential utility of combining FP-CBCT scanning with fluoroscopy to position surgical tools when stereotactic devices and image-guided surgery systems are not available. However, further work is required to fully characterize the precision and accuracy of the method in a variety of realistic surgical sites.

Improving the detection of osteosarcoma tumor margins and metastasis using diagnostic nanoparticles

2009 ◽  
Vol 27 (15_suppl) ◽  
pp. 10512-10512
Author(s):  
A. Dutour ◽  
A. Decouveleare ◽  
V. Josserand ◽  
J. Coll ◽  
F. Chotel ◽  
...  
Keyword(s):  
Near Infrared ◽  
Intraoperative Imaging ◽  
Malignant Lesion ◽  
Surgical Excision ◽  
Complete Removal ◽  
Preoperative Imaging ◽  
Tumor Control ◽  
Fluorescent Nanoparticles ◽  
Metastatic Osteosarcoma ◽  
Imaging Results

10512 Background: The aim of osteosarcoma resection is the complete removal of the primary malignant lesion with adequate margins taking into account tumor control and functional reconstruction. To date, no intraoperative method is available to assist surgeons in precisely delineating tumor extension in the adjacent normal tissues. Thus, preoperative imaging and extended surgical resection remains the gold standard. We developed fluorescent nanoparticles RAFT-cRGD4-ICG’ targeting αVβ3 integrins on tumor neovessels and demonstrated their capacity to reveal tumor and its margins when exposed to near infrared (NIR) light. Methods: We evaluated in an orthotopic metastatic osteosarcoma in rats the potential of RAFT-cRGD4-ICG’ detected intra operatively by NIR illumination to improve margin resection without compromising local tumor control. Controls consisted of pathological margin analysis of the resected tumor/metastasis comparing ICG’ fluorescence with conventional tissue coloration, and pre operative magnetic resonance imaging. Results: We showed a significantly lesser extent of healthy tissue resection after surgical excision when assessing tumor margin intra operatively using RAFT-cRGD4-ICG’ and NIR camera compared to preoperative imaging studies and post operative conventional pathology coloration (p<0.01). Importantly, intraoperative NIR illumination of lungs revealed more metastases than by intraoperative manual lung palpation (p<0.01). Conclusions: Our preclinical data confirm the potential of intraoperative imaging for improved primary tumor and lung metastasis excision. We are now generating clinical-grade RAFT-cRGD4-ICG’ to further evaluate this approach as a mean to improve functional outcome after surgical excision while maintaining tumor control in patients with relapsed/metastatic osteosarcoma. No significant financial relationships to disclose.

Logical Fallacies and Misinterpretations that Hinder Progress in Translational Addiction Neuroscience

2022 ◽  
Author(s):  
Justin Charles Strickland ◽  
William Stoops ◽  
Matthew &gt; Banks ◽  
Cassandra D. Gipson-Reichardt
Keyword(s):  
Animal Models ◽  
Theoretical Orientation ◽  
Behavioral Models ◽  
Preclinical Models ◽  
Case Examples ◽  
Affirming The Consequent ◽  
Logical Fallacies ◽  
Circular Explanation ◽  
The Brain ◽  
Human Primate

Substance use disorders (SUDs) are heterogenous and complex, making the development of translationally predictive rodent and non-human primate models to uncover their neurobehavioral underpinnings difficult. Neuroscience-focused outcomes have become highly prevalent, and with this, the notion that SUDs are disorders of the brain embraced as a dominant theoretical orientation to understand SUD etiology and treatment. These efforts, however, have led to few efficacious pharmacotherapies, and in some cases (as with cocaine or methamphetamine), no pharmacotherapies have translated from preclinical models for clinical use. In this review and theoretical commentary, we first describe the development of animal models of SUDs from a historical perspective. We then define and discuss three logical fallacies including 1) circular explanation, 2) affirming the consequent, and 3) reification that can apply to developed models. We then provide three case examples in which conceptual or logical issues exist in common methods (i.e., behavioral economic demand, escalation, and reinstatement). Alternative strategies to refocus behavioral models are suggested for the field in an attempt to better bridge the translational divide between animal models and the clinical condition of SUDs.

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