scholarly journals Historical phenotypic data from seven decades of seed regeneration in a wheat ex situ collection

2019 ◽  
Vol 6 (1) ◽  
Author(s):  
Norman Philipp ◽  
Stephan Weise ◽  
Markus Oppermann ◽  
Andreas Börner ◽  
Jens Keilwagen ◽  
...  
Keyword(s):  
Ex Situ ◽  
Phenotypic Data ◽  
Seed Regeneration ◽  
Ex Situ Collection

scholarly journals Unbalanced historical phenotypic data from seed regeneration of a barley ex situ collection

2018 ◽  
Vol 5 (1) ◽  
Author(s):  
Maria Y. Gonzalez ◽  
Stephan Weise ◽  
Yusheng Zhao ◽  
Norman Philipp ◽  
Daniel Arend ◽  
...  
Keyword(s):  
Genetic Resources ◽  
Phenotypic Diversity ◽  
Spring Barley ◽  
Plant Genetic Resources ◽  
Crop Improvement ◽  
Model Organism ◽  
Ex Situ ◽  
Phenotypic Characterization ◽  
Phenotypic Data ◽  
Seed Regeneration

Abstract The scarce knowledge on phenotypic characterization restricts the usage of genetic diversity of plant genetic resources in research and breeding. We describe original and ready-to-use processed data for approximately 60% of ~22,000 barley accessions hosted at the Federal ex situ Genebank for Agricultural and Horticultural Plant Species. The dataset gathers records for three traits with agronomic relevance: flowering time, plant height and thousand grain weight. This information was collected for seven decades for winter and spring barley during the seed regeneration routine. The curated data represent a source for research on genetics and genomics of adaptive and yield related traits in cereals due to the importance of barley as model organism. This data could be used to predict the performance of non-phenotyped individuals in other collections through genomic prediction. Moreover, the dataset empowers the utilization of phenotypic diversity of genetic resources for crop improvement.

scholarly journals Genebank Phenomics: A Strategic Approach to Enhance Value and Utilization of Crop Germplasm

Plants ◽  
2020 ◽  
Vol 9 (7) ◽  
pp. 817 ◽  
Author(s):  
Giao N. Nguyen ◽  
Sally L. Norton
Keyword(s):  
High Throughput ◽  
Cost Effective ◽  
Screening Tools ◽  
Ex Situ ◽  
Phenotypic Data ◽  
Germplasm Collections ◽  
Digital Phenotyping ◽  
Seed Regeneration ◽  
Novel Alleles ◽  
Crop Germplasm

Genetically diverse plant germplasm stored in ex-situ genebanks are excellent resources for breeding new high yielding and sustainable crop varieties to ensure future food security. Novel alleles have been discovered through routine genebank activities such as seed regeneration and characterization, with subsequent utilization providing significant genetic gains and improvements for the selection of favorable traits, including yield, biotic, and abiotic resistance. Although some genebanks have implemented cost-effective genotyping technologies through advances in DNA technology, the adoption of modern phenotyping is lagging. The introduction of advanced phenotyping technologies in recent decades has provided genebank scientists with time and cost-effective screening tools to obtain valuable phenotypic data for more traits on large germplasm collections during routine activities. The utilization of these phenotyping tools, coupled with high-throughput genotyping, will accelerate the use of genetic resources and fast-track the development of more resilient food crops for the future. In this review, we highlight current digital phenotyping methods that can capture traits during annual seed regeneration to enrich genebank phenotypic datasets. Next, we describe strategies for the collection and use of phenotypic data of specific traits for downstream research using high-throughput phenotyping technology. Finally, we examine the challenges and future perspectives of genebank phenomics.

Unlocking historical phenotypic data from an ex situ collection to enhance the informed utilization of genetic resources of barley (Hordeum sp.)

2018 ◽  
Vol 131 (9) ◽  
pp. 2009-2019 ◽  
Author(s):  
Maria Y. González ◽  
Norman Philipp ◽  
Albert W. Schulthess ◽  
Stephan Weise ◽  
Yusheng Zhao ◽  
...  
Keyword(s):  
Genetic Resources ◽  
Ex Situ ◽  
Phenotypic Data ◽  
Ex Situ Collection

scholarly journals Diversity and Relationships among Neglected Apricot (Prunus armeniaca L.) Landraces Using Morphological Traits and SSR Markers: Implications for Agro-Biodiversity Conservation

Plants ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 1341
Author(s):  
Giandomenico Corrado ◽  
Marcello Forlani ◽  
Rosa Rao ◽  
Boris Basile
Keyword(s):  
Prunus Armeniaca ◽  
Ex Situ ◽  
Skin Colour ◽  
Maintenance Breeding ◽  
Prunus Armeniaca L ◽  
Ex Situ Collection ◽  
Loss Of Diversity ◽  
Ssrs Markers ◽  
On Farm ◽  
Simple Sequence

Apricot (Prunus armeniaca L.) is an economically important tree species globally cultivated in temperate areas. Italy has an ample number of traditional varieties, but numerous landraces are abandoned and at risk of extinction because of increasing urbanization, agricultural intensification, and varietal renewal. In this work, we investigated the morphological and genetic diversity present in an ex-situ collection of 28 neglected varieties belonging to the so-called “Vesuvian apricot”. Our aim was to understand the level of diversity and the possible link between the promotion of specific fruit types (e.g., by public policies) and the intraspecific variation in apricot. The combination of five continuous and seven categorical traits allowed us to phenotypically distinguish the varieties; while fruit quality-related attributes displayed high variation, both apricot size and skin colour were more uniform. The twelve fluorescent-based Simple Sequence Repeats (SSRs) markers identified cultivar-specific molecular profiles and revealed a high molecular diversity, which poorly correlated with that described by the morphological analysis. Our results highlighted the complementary information provided by the two sets of descriptors and that DNA markers are necessary to separate morphologically related apricot landraces. The observed morphological and genetic differences suggest a loss of diversity influenced by maintenance breeding of specific pomological traits (e.g., skin colour and size). Finally, our study provided evidence to recommend complementary strategies to avoid the loss of diversity in apricot. Actions should pivot on both the promotion of easily identified premium products and more inclusive biodiversity-centred on-farm strategies.

scholarly journals Pengelolaan Sumber Daya Genetik Tanaman Obat Spesifik Kalimantan Tengah

2016 ◽  
Vol 12 (1) ◽  
pp. 16 ◽  
Author(s):  
Amik Krismawati ◽  
M. Sabran
Keyword(s):  
Medicinal Plants ◽  
Ex Situ Conservation ◽  
Coastal Region ◽  
Ex Situ ◽  
Central Kalimantan ◽  
Acid Sulphate ◽  
Medicinal Crops ◽  
Continental Area ◽  
Ex Situ Collection

<p>Central Kalimantan Province covers an area of 15,38 million ha which consist of 61,140 ha coastal region, 1,53 million ha open waters, and 13,79 million ha as inland area. The continental area consisted of various type of lands e.g. acid sulphate, peats soil, and up land. In order to explore and conserve potential medicinal plants indigenous to Central Kalimantan, these exploration and characterization activities were conducted. Method of study: (1) exploration, (2) ex situ conservation, (3) characterization, and (4) documentation. The result of these activities are ex situ collection of 15 acessions of medicinal crops. This exploration obtained 15 accessions of medicinal plants which were characterized and conserved in ex situ condition.</p><p> </p><p><strong>Abstrak</strong></p><p>Kalimantan Tengah memiliki luas wilayah 15,38 juta ha yang terdiri dari 61,1 ribu ha daerah pantai, 1,53 juta ha daerah perairan umum, dan 17,79 juta ha daratan. Wilayah daratan yang luas ini terdiri atas berbagai tipologi lahan seperti lahan sulfat masam, gambut, dan lahan kering. Untuk menggali potensi dan melestarikan plasma nutfah tanaman obat spesifik Kalimantan Tengah telah dilaksanakan kegiatan eksplorasi dan karakterisasi. Dari kegiatan ini dapat disusun deskripsi berbagai jenis tanaman tersebut untuk inventarisasi, karakterisasi, dan koleksi tanaman obat secara ex situ dalam bentuk kebun pembibitan. Metode kegiatan meliputi (1) eksplorasi, (2) konservasi ex situ, (3) karakterisasi, dan (4) dokumentasi. Hasil kegiatan adalah koleksi secara ex situ tanaman obat sebanyak 15 aksesi.</p>

Morphological variability of prickly pear cultivars (Opuntia spp.) established in ex-situ collection in Tunisia

2019 ◽  
Vol 248 ◽  
pp. 163-175 ◽  
Author(s):  
Elhani Amani ◽  
Louati Marwa ◽  
Ben Salem Hichem ◽  
Salhi-Hannachi Amel ◽  
Baraket Ghada
Keyword(s):  
Morphological Variability ◽  
Prickly Pear ◽  
Ex Situ ◽  
Ex Situ Collection ◽  
Opuntia Spp

Searching an Ex Situ Collection of Wheat Genetic Resources

2000 ◽  
Vol 82 (4) ◽  
pp. 812-827 ◽  
Author(s):  
Douglas Gollin ◽  
Melinda Smale ◽  
Bent Skovmand
Keyword(s):  
Genetic Resources ◽  
Ex Situ ◽  
Ex Situ Collection

scholarly journals Genetic Diversity and Population Structure of Algerian Endemic Plant Species Avena macrostachya Bal. ex Cross. et Durieu

Agronomy ◽  
2020 ◽  
Vol 10 (12) ◽  
pp. 1984
Author(s):  
Paulina Bolc ◽  
Bogusław Łapiński ◽  
Wiesław Podyma ◽  
Maja Boczkowska
Keyword(s):  
Genetic Diversity ◽  
Population Structure ◽  
Ex Situ ◽  
Small Scale ◽  
Endemic Plant ◽  
Unknown Species ◽  
Close Relationship ◽  
Ex Situ Collection ◽  
Endemic Plant Species ◽  
Inexpensive Technique

Avena macrostachya is still a relatively unknown species. Using sequence-related amplified polymorphism (SRAP) markers, a simple and inexpensive technique, allowed us to conduct experiments on genetic differentiation and to study the population structure of this Algerian endemic oat. The results obtained showed lower than expected genetic diversity within the A. macrostachya species. The cause may be endemism of the species as well as genetic drift possible during collection, and maintenance of the accessions in gene bank and seed reproduction. No clear genetic structure was found in the examined collection, which indicates a close relationship between the populations collected in the Djurdjura National Park in Algeria. Considering the endemism of the species, its breeding potential and the small-scale ex situ collection, careful monitoring of natural sites and repeating of the collection mission are, therefore, absolutely crucial.

scholarly journals Phenotypic diversity assessment within a major ex situ collection of wild endemic coffees in Madagascar

2020 ◽  
Vol 126 (5) ◽  
pp. 849-863
Author(s):  
Aurore Rimlinger ◽  
Nathalie Raharimalala ◽  
Véronique Letort ◽  
Jean-Jacques Rakotomalala ◽  
Dominique Crouzillat ◽  
...  
Keyword(s):  
Phenotypic Diversity ◽  
Phylogenetic Signal ◽  
Natural Habitat ◽  
Ex Situ ◽  
Dry Matter Content ◽  
Natural Habitats ◽  
Leaf Mass ◽  
Content Ratio ◽  
Diversity Assessment ◽  
Ex Situ Collection

Abstract Background and Aims Like other clades, the Coffea genus is highly diversified on the island of Madagascar. The 66 endemic species have colonized various environments and consequently exhibit a wide diversity of morphological, functional and phenological features and reproductive strategies. The trends of interspecific trait variation, which stems from interactions between genetically defined species and their environment, still needed to be addressed for Malagasy coffee trees. Methods Data acquisition was done in the most comprehensive ex situ collection of Madagascan wild Coffea. The structure of endemic wild coffees maintained in an ex situ collection was explored in terms of morphological, phenological and functional traits. The environmental (natural habitat) effect was assessed on traits in species from distinct natural habitats. Phylogenetic signal (Pagel’s λ, Blomberg’s K) was used to quantify trait proximities among species according to their phylogenetic relatedness. Key Results Despite the lack of environmental difference in the ex situ collection, widely diverging phenotypes were observed. Phylogenetic signal was found to vary greatly across and even within trait categories. The highest values were exhibited by the ratio of internode mass to leaf mass, the length of the maturation phase and leaf dry matter content (ratio of dry leaf mass to fresh leaf mass). By contrast, traits weakly linked to phylogeny were either constrained by the original natural environment (leaf size) or under selective pressures (phenological traits). Conclusions This study gives insight into complex patterns of trait variability found in an ex situ collection, and underlines the opportunities offered by living ex situ collections for research characterizing phenotypic variation.

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