Publisher Correction: Microbial network of the carbonate precipitation process induced by microbial consortia and the potential application to crack healing in concrete

Chain elongation of volatile fatty acids for medium chain fatty acids production (e.g. caproate) is an attractive approach to treat wastewater anaerobically and recover resource simultaneously. Undefined microbial consortia can be tailored to achieve chain elongation process with selective enrichment from anaerobic digestion sludge, which has advantages over pure culture approach for cost-efficient application. Whilst the metabolic pathway of the dominant caproate producer, Clostridium kluyveri, has been annotated, the role of other coexisting abundant microbiomes remained unclear. To this end, an ethanol-acetate fermentation inoculated with fresh digestion sludge at optimal conditions was conducted. Also, physiological study, thermodynamics and 16 S rRNA gene sequencing to elucidate the biological process by linking the system performance and dominant microbiomes were integrated. Results revealed a possible synergistic network in which C. kluyveri and three co-dominant species, Desulfovibrio vulgaris, Fusobacterium varium and Acetoanaerobium sticklandii coexisted. D. vulgaris and A. sticklandii (F. varium) were likely to boost the carboxylates chain elongation by stimulating ethanol oxidation and butyrate production through a syntrophic partnership with hydrogen (H2) serving as an electron messenger. This study unveils a synergistic microbial network to boost caproate production in mixed culture carboxylates chain elongation.

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A sample cell for the study of enzyme-induced carbonate precipitation at the grain-scale and its implications for biocementation

Scientific Reports ◽

10.1038/s41598-021-92235-7 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Jennifer Zehner ◽

Anja Røyne ◽

Pawel Sikorski

Keyword(s):

Real Time ◽

Laser Scanning Microscopy ◽

In Situ Monitoring ◽

Confocal Laser ◽

Precipitation Process ◽

Successful Implementation ◽

Detailed Knowledge ◽

Carbonate Precipitation ◽

Sample Cell

AbstractBiocementation is commonly based on microbial-induced carbonate precipitation (MICP) or enzyme-induced carbonate precipitation (EICP), where biomineralization of $$\text {CaCO}_{3}$$ CaCO 3 in a granular medium is used to produce a sustainable, consolidated porous material. The successful implementation of biocementation in large-scale applications requires detailed knowledge about the micro-scale processes of $$\text {CaCO}_{3}$$ CaCO 3 precipitation and grain consolidation. For this purpose, we present a microscopy sample cell that enables real time and in situ observations of the precipitation of $$\text {CaCO}_{3}$$ CaCO 3 in the presence of sand grains and calcite seeds. In this study, the sample cell is used in combination with confocal laser scanning microscopy (CLSM) which allows the monitoring in situ of local pH during the reaction. The sample cell can be disassembled at the end of the experiment, so that the precipitated crystals can be characterized with Raman microspectroscopy and scanning electron microscopy (SEM) without disturbing the sample. The combination of the real time and in situ monitoring of the precipitation process with the possibility to characterize the precipitated crystals without further sample processing, offers a powerful tool for knowledge-based improvements of biocementation.

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Novel Fe3O4/HNT@rGO composite via a facile co-precipitation method for the removal of contaminants from aqueous system

RSC Advances ◽

10.1039/c6ra01279e ◽

2016 ◽

Vol 6 (54) ◽

pp. 49228-49235 ◽

Cited By ~ 10

Author(s):

Chengwei Gao ◽

Baojun Li ◽

Ning Chen ◽

Jie Ding ◽

Qiang Cai ◽

...

Keyword(s):

Heat Treatment ◽

Water Treatment ◽

Potential Application ◽

High Performance ◽

Aqueous System ◽

Precipitation Method ◽

Precipitation Process ◽

System P ◽

Removal Of Contaminants ◽

Co Precipitation

Fe3O4/HNT@rGO composite (FHGC) was fabricated via a facile co-precipitation process, followed by heat treatment. For RhB and As5+removal, the high performance and easy separation of FHGC highlight its potential application in water treatment.

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Potential application of developed methanogenic microbial consortia for coal biogasification

International Journal of Coal Geology ◽

10.1016/j.coal.2018.02.013 ◽

2018 ◽

Vol 188 ◽

pp. 165-180 ◽

Cited By ~ 9

Author(s):

John Fuertez ◽

Gloria Córdoba ◽

John D. McLennan ◽

D. Jack Adams ◽

Taylor D. Sparks

Keyword(s):

Potential Application ◽

Microbial Consortia

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Calcite seed-assisted microbial induced carbonate precipitation (MICP) and its potential in biocementation

10.1101/2020.07.17.206516 ◽

2020 ◽

Author(s):

Jennifer Zehner ◽

Anja Røyne ◽

Pawel Sikorski

Keyword(s):

Calcium Carbonate ◽

Crystallization Process ◽

Biological Process ◽

Precipitation Process ◽

Carbonate Precipitation ◽

Amorphous Calcium Carbonate ◽

Calcium Carbonate Precipitation ◽

Mineral Precipitation ◽

Solid Materials ◽

Significant Difference

Microbial-induced calcium carbonate precipitation (MICP) is a biological process inducing biomineralization of CaCO3. This can be used to form a solid, concrete-like material. To be able to use MICP successfully for producing solid materials, it is important to understand the formation process of the material in detail. It is well known, that crystallization surfaces can influence the precipitation process. Therefore, we present in this contribution a systematic study investigating the influence of calcite seeds on the MICP processes. We focus on the pH changes during the crystallization process measured with absorption spectroscopy and on the optical density (OD) signal to analyze the precipitation process. Furthermore, optical microscopy was used to visualize the precipitation processes in the sample and connect them to changes in pH and OD. We show that there is a significant difference in the pH evolution between samples with and without calcite seeds present and that the shape of the pH evolution and the changes in OD can give detailed information about the mineral precipitation and transformations. In the presented experiments we show that amorphous calcium carbonate (ACC) can also precipitate in the presence of initial calcite seeds, which can have consequences for consolidated MICP materials.

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Crack Healing in Cementitious Mortars Using Enzyme-Induced Carbonate Precipitation: Quantification Based on Fracture Response

Journal of Materials in Civil Engineering ◽

10.1061/(asce)mt.1943-5533.0002218 ◽

2018 ◽

Vol 30 (4) ◽

pp. 04018035 ◽

Cited By ~ 12

Author(s):

Akash Dakhane ◽

Sumanta Das ◽

Hannah Hansen ◽

Sean O’Donnell ◽

Farouq Hanoon ◽

...

Keyword(s):

Carbonate Precipitation ◽

Crack Healing

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Effect of Temperature on Calcium Carbonate Precipitation in Biomimetic Calcium Chloride Solution

Advances in Science and Technology ◽

10.4028/www.scientific.net/ast.107.76 ◽

2021 ◽

Vol 107 ◽

pp. 76-81

Author(s):

Intan Nurfarzana Mohd Razib Fatheen ◽

Che Husain Syuhani ◽

Hamzah Fazlena ◽

Najwa Mohd Rodhi Miradatul ◽

Veny Harumi

Keyword(s):

Calcium Carbonate ◽

Calcium Chloride ◽

Chloride Solution ◽

Calcium Chloride Solution ◽

Effect Of Temperature ◽

Precipitation Process ◽

Carbonate Precipitation ◽

Calcium Carbonate Precipitation ◽

Calcium Carbonate Precipitate ◽

Temperature Range

In the present work, the effect of temperature on calcium carbonate precipitation in the biomimetic calcium chloride solution was investigated. A spontaneous calcium carbonate precipitate was formed in the biomimetic calcium chloride solution as a result of the carbon dioxide hydration process. The reaction was conducted at different temperature range vary from 30°C to 100°C. The mass of the calcium carbonate precipitate and the pH solution was measured in the study. The finding indicated that an increment of the temperature has led to the fast pH reduction of the solutions to 7.0. However, the process has retarded the calcium carbonate precipitation process. The optimum temperature for higher calcium carbonate precipitation has occurred at the temperature range of 47.5°C – 65°C which gave the highest calcium carbonate precipitate at 0.121g. The addition of Tris buffer into the calcium chloride solution in this study did not gave an inhibition effect on the calcium carbonate precipitate. Based on the results, an operating condition at 47.5°C – 65°C was recommended to be used in mineral carbonization of CO2 using the biomimetic calcium chloride solution.

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