scholarly journals Spatiotemporal observation of light propagation in a three-dimensional scattering medium

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Tomoyoshi Inoue ◽  
Yuasa Junpei ◽  
Seiya Itoh ◽  
Tatsuya Okuda ◽  
Akinori Funahashi ◽  
...  
Keyword(s):  
Optical Imaging ◽  
Temporal Resolution ◽  
Light Pulse ◽  
Pulse Propagation ◽  
Imaging Techniques ◽  
Three Dimensional ◽  
Optical Scattering ◽  
Scattering Medium ◽  
Ultrashort Light Pulse ◽  
Ultrafast Optical

AbstractSpatiotemporal information about light pulse propagation obtained with femtosecond temporal resolution plays an important role in understanding transient phenomena and light–matter interactions. Although ultrafast optical imaging techniques have been developed, it is still difficult to capture light pulse propagation spatiotemporally. Furthermore, imaging through a three-dimensional (3-D) scattering medium is a longstanding challenge due to the optical scattering caused by the interaction between light pulse and a 3-D scattering medium. Here, we propose a technique for ultrafast optical imaging of light pulses propagating inside a 3D scattering medium. We record an image of the light pulse propagation using the ultrashort light pulse even when the interaction between light pulse and a 3-D scattering medium causes the optical scattering. We demonstrated our proposed technique by recording converging, refracted, and diffracted propagating light for 59 ps with femtosecond temporal resolution.

Mathematical simulation of ultrashort light pulse propagation in a highly scattering medium

2010 ◽  
Vol 2 (1) ◽  
pp. 22-32 ◽  
Author(s):  
V. S. Kuznetsov ◽  
O. V. Nikolaeva ◽  
L. P. Bass ◽  
A. V. Bykov ◽  
A. V. Priezzhev
Keyword(s):  
Mathematical Simulation ◽  
Light Pulse ◽  
Pulse Propagation ◽  
Scattering Medium ◽  
Ultrashort Light Pulse ◽  
Highly Scattering Medium

Moving picture recording and reconstruction of visible ultrashort light pulse propagation in diffusive medium

2009 ◽  
Author(s):  
Yasuhiro Awatsuji ◽  
Tetsuya Takimoto ◽  
Shinichi Nakajima ◽  
Masahiro Makino ◽  
Kazuya Tosa ◽  
...  
Keyword(s):  
Light Pulse ◽  
Pulse Propagation ◽  
Ultrashort Light Pulse ◽  
Diffusive Medium

scholarly journals Neuroanatomical phenotyping of the mouse brain with three-dimensional autofluorescence imaging

2012 ◽  
Vol 44 (15) ◽  
pp. 778-785 ◽  
Author(s):  
Jacqueline A. Gleave ◽  
Michael D. Wong ◽  
Jun Dazai ◽  
Maliha Altaf ◽  
R. Mark Henkelman ◽  
...  
Keyword(s):  
Optical Imaging ◽  
Mouse Brain ◽  
Imaging Techniques ◽  
Three Dimensional ◽  
Brain Structures ◽  
Small Scale ◽  
Specimen Preparation ◽  
Normal Brain ◽  
Autofluorescence Imaging ◽  
The Brain

The structural organization of the brain is important for normal brain function and is critical to understand in order to evaluate changes that occur during disease processes. Three-dimensional (3D) imaging of the mouse brain is necessary to appreciate the spatial context of structures within the brain. In addition, the small scale of many brain structures necessitates resolution at the ∼10 μm scale. 3D optical imaging techniques, such as optical projection tomography (OPT), have the ability to image intact large specimens (1 cm3) with ∼5 μm resolution. In this work we assessed the potential of autofluorescence optical imaging methods, and specifically OPT, for phenotyping the mouse brain. We found that both specimen size and fixation methods affected the quality of the OPT image. Based on these findings we developed a specimen preparation method to improve the images. Using this method we assessed the potential of optical imaging for phenotyping. Phenotypic differences between wild-type male and female mice were quantified using computer-automated methods. We found that optical imaging of the endogenous autofluorescence in the mouse brain allows for 3D characterization of neuroanatomy and detailed analysis of brain phenotypes. This will be a powerful tool for understanding mouse models of disease and development and is a technology that fits easily within the workflow of biology and neuroscience labs.

scholarly journals Optical imaging and modulation of neurovascular responses

2018 ◽  
Vol 38 (12) ◽  
pp. 2057-2072 ◽  
Author(s):  
Kazuto Masamoto ◽  
Alberto Vazquez
Keyword(s):  
Optical Imaging ◽  
Vascular Function ◽  
Imaging Techniques ◽  
Three Dimensional ◽  
Brain Regions ◽  
Transgenic Animal ◽  
Optical Methods ◽  
Vascular Networks ◽  
Temporal Properties ◽  
Dimensional Reconstruction

The cerebral microvasculature consists of pial vascular networks, parenchymal descending arterioles, ascending venules and parenchymal capillaries. This vascular compartmentalization is vital to precisely deliver blood to balance continuously varying neural demands in multiple brain regions. Optical imaging techniques have facilitated the investigation of dynamic spatial and temporal properties of microvascular functions in real time. Their combination with transgenic animal models encoding specific genetic targets have further strengthened the importance of optical methods for neurovascular research by allowing for the modulation and monitoring of neuro vascular function. Image analysis methods with three-dimensional reconstruction are also helping to understand the complexity of microscopic observations. Here, we review the compartmentalized cerebral microvascular responses to global perturbations as well as regional changes in response to neural activity to highlight the differences in vascular action sites. In addition, microvascular responses elicited by optical modulation of different cell-type targets are summarized with emphasis on variable spatiotemporal dynamics of microvascular responses. Finally, long-term changes in microvascular compartmentalization are discussed to help understand potential relationships between CBF disturbances and the development of neurodegenerative diseases and cognitive decline.

scholarly journals Multi-color imaging of sub-mitochondrial structures in living cells using structured illumination microscopy

Nanophotonics ◽  
2018 ◽  
Vol 7 (5) ◽  
pp. 935-947 ◽  
Author(s):  
Ida S. Opstad ◽  
Deanna L. Wolfson ◽  
Cristina I. Øie ◽  
Balpreet S. Ahluwalia
Keyword(s):  
Optical Imaging ◽  
Mitochondrial Dynamics ◽  
Imaging Techniques ◽  
Three Dimensional ◽  
Time Lapse ◽  
Living Cells ◽  
Intermembrane Space ◽  
Structured Illumination ◽  
Structured Illumination Microscopy ◽  
Microscopy Techniques

AbstractThe dimensions of mitochondria are close to the diffraction limit of conventional light microscopy techniques, making the complex internal structures of mitochondria unresolvable. In recent years, new fluorescence-based optical imaging techniques have emerged, which allow for optical imaging below the conventional limit, enabling super-resolution (SR). Possibly the most promising SR and diffraction-limited microscopy techniques for live-cell imaging are structured illumination microscopy (SIM) and deconvolution microscopy (DV), respectively. Both SIM and DV are widefield techniques and therefore provide fast-imaging speed as compared to scanning based microscopy techniques. We have exploited the capabilities of three-dimensional (3D) SIM and 3D DV to investigate different sub-mitochondrial structures in living cells: the outer membrane, the intermembrane space, and the matrix. Using different mitochondrial probes, each of these sub-structures was first investigated individually and then in combination. We describe the challenges associated with simultaneous labeling and SR imaging and the optimized labeling protocol and imaging conditions to obtain simultaneous three-color SR imaging of multiple mitochondrial regions in living cells. To investigate both mitochondrial dynamics and structural details in the same cell, the combined usage of DV for long-term time-lapse imaging and 3D SIM for detailed, selected time point analysis was a useful strategy.

scholarly journals Single-shot stereo-polarimetric compressed ultrafast photography for light-speed observation of high-dimensional optical transients with picosecond resolution

2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Jinyang Liang ◽  
Peng Wang ◽  
Liren Zhu ◽  
Lihong V. Wang
Keyword(s):  
Optical Imaging ◽  
Linear Polarization ◽  
Early Stage ◽  
Imaging Techniques ◽  
Video Recording ◽  
Three Dimensional ◽  
High Dimensional ◽  
Time Of Arrival ◽  
Single Shot ◽  
Light Speed

Abstract Simultaneous and efficient ultrafast recording of multiple photon tags contributes to high-dimensional optical imaging and characterization in numerous fields. Existing high-dimensional optical imaging techniques that record space and polarization cannot detect the photon’s time of arrival owing to the limited speeds of the state-of-the-art electronic sensors. Here, we overcome this long-standing limitation by implementing stereo-polarimetric compressed ultrafast photography (SP-CUP) to record light-speed high-dimensional events in a single exposure. Synergizing compressed sensing and streak imaging with stereoscopy and polarimetry, SP-CUP enables video-recording of five photon tags (x, y, z: space; t: time of arrival; and ψ: angle of linear polarization) at 100 billion frames per second with a picosecond temporal resolution. We applied SP-CUP to the spatiotemporal characterization of linear polarization dynamics in early-stage plasma emission from laser-induced breakdown. This system also allowed three-dimensional ultrafast imaging of the linear polarization properties of a single ultrashort laser pulse propagating in a scattering medium.

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